Abstract：
:Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the deoxyoligonucleotides D1 and 5'-d(AAATATTTAAAATTA-(T)10]-3' (D3). The Raman spectra of the antiparallel-stranded (aps) duplex are reminiscent of the spectra of poly[d(A)].poly[d(T)] and a B-form structure similar to that adopted by the homopolymer duplex is assigned to the antiparallel double helix. The spectra of the parallel-stranded (ps) and antiparallel-stranded duplexes differ significantly due to changes in helical organization, i.e., base pairing, base stacking, and backbone conformation. Large changes observed in the carbonyl stretching region (1600-1700 cm-1) implicate the involvement of the C(2) carbonyl of thymine in base pairing. The interaction of adenine with the C(2) carbonyl of thymine is consistent wtih formation of reverse Watson-Crick base pairing in parallel-stranded DNA. Phosphate-furanose vibrations similar to those observed for B-form DNA of heterogenous sequence and high A,T content are observed at 843 and 1092 cm-1 in the spectra of the parallel-stranded duplex. The 843-cm-1 band is due to the presence of a sizable population of furanose rings in the C2'-endo conformation. Significant changes observed in the regions from 1150 to 1250 cm-1 and from 1340 to 1400 cm-1 in the spectra of the parallel-stranded duplex are attributed to variations in backbone torsional and glycosidic angles and base stacking.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Otto C,Thomas GA,Rippe K,Jovin TM,Peticolas WLdoi
10.1021/bi00226a012subject
Has Abstractpub_date
1991-03-26 00:00:00pages
3062-9issue
12eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Several 3-halo-3-(1-haloalkyl)-1(3H)-isobenzofuranones, 3-(1-haloalkylidene)-1(3H)-isobenzofuranones, and 3-bromomethyl-1H-2-benzopyran-1-ones containing masked halo ketone functional groups were synthesized and tested as inhibitors of several serine proteases including human leukocyte (HL) elastase and cathepsin G. W...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a006
更新日期：1985-04-09 00:00:00
abstract：:We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952547+
更新日期：1996-04-16 00:00:00
abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9725069
更新日期：1998-05-12 00:00:00
abstract：:Mitochondria act as a focal point for upstream apoptosis signals by releasing cytochrome c into the cytosol, leading to the activation of caspases and subsequent cell death. Members of the Bcl-2 protein family regulate this phenomenon by heterodimerization via the BH3 domain of proapoptotic members opposing their pro-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0110286
更新日期：2002-07-09 00:00:00
abstract：:Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701311c
更新日期：2007-11-20 00:00:00
abstract：:Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051267j
更新日期：2005-10-25 00:00:00
abstract：:Depletion of functional Ca2+ from photosystem (PS) II membranes impairs O2 evolution. Redox properties of the Mn cluster as probed by thermoluminescence were modified differently in Ca(2+)-depleted PSII depending on the procedure for Ca2+ extraction. Ca2+ depletion by low-pH treatment gave rise to an abnormally modifi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00148a028
更新日期：1992-08-25 00:00:00
abstract：:The S100 proteins comprise a group of EF-hand proteins that undergo a calcium-induced conformational change allowing them to interact with other proteins and produce a biological response. A unique feature of these proteins is the fact that they can form both homo- and heterodimers independent of calcium binding. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802086a
更新日期：2009-03-10 00:00:00
abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981517e
更新日期：1998-10-27 00:00:00
abstract：:Determining the sequence of bond cleavages, and consequently the nature of intermediates, in enzyme-catalyzed reactions is a major goal of mechanistic enzymology. When significant primary isotope effects on V/K are observed for two different bond cleavages, both bonds may be broken in the same transition state or they...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00238a013
更新日期：1991-06-18 00:00:00
abstract：:Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, named protein B1 (170 000) and protein B2 (87 000). We purified and characterized five monoclonal antibodies against B1 and three against B2 from hybridomas obtained by fusion of spleen cells from immunized mice and the myeloma cell ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00352a018
更新日期：1986-02-25 00:00:00
abstract：:Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that phosphorylates and inactivates eEF-2 and that can regulate the rate of protein synthesis at the elongation stage. Here we report that a slight decrease in pH, within the range observed in vivo, leads to a dramatic activation of eE...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026494p
更新日期：2002-11-12 00:00:00
abstract：:Recent studies have indicated that CYP3A4 exhibits non-Michaelis-Menten kinetics for numerous substrates. Both homo- and heterotropic activation have been reported, and kinetic models have suggested multiple substrates within the active site. We provide some of the first physicochemical data supporting the hypothesis ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051689t
更新日期：2005-11-01 00:00:00
abstract：:We have studied the properties and structures of a series of Escherichia coli RNA polymerase ternary complexes formed during the initial steps of RNA chain initiation and elongation. Five different templates were used that contained the bacteriophage T7 A1 promoter or the E. coli Tac or the lac UV5 promoter, as well a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a045
更新日期：1989-09-19 00:00:00
abstract：:The Plasmodium falciparum malarial parasite genome appears to encode one and only one phosphatidylinositol 3'-kinase (PI3K), and sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as a possible druggable target and potentially a key target of artemisinin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00416
更新日期：2017-08-22 00:00:00
abstract：:We studied the urea-induced unfolding transition of staphylococcal nuclease (SNase) and its five proline mutants (P47A, P47T, P117G, P47T/P117G, and P47A/P117G) [corrected] by peptide and aromatic circular dichroism and aromatic absorption spectroscopy at equilibrium and the refolding-unfolding kinetics of the protein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963174v
更新日期：1997-05-27 00:00:00
abstract：:We have used nucleobase substitution and kinetic analysis to test the hypothesis that hammerhead catalysis occurs by a general acid-base mechanism, in which nucleobases are directly involved in deprotonation of the attacking 2'-hydroxyl group and protonation of the 5'-oxygen that serves as the leaving group in the cle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047941z
更新日期：2005-05-31 00:00:00
abstract：:Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra- and extracellular Cs+, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a003
更新日期：1988-05-17 00:00:00
abstract：:Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated. Proteoliposomes containing purified MelB were adsorbed to a solid supported lipid membrane, activated by a substrate concentration jump, and transient currents were measured. When ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0489053
更新日期：2004-10-05 00:00:00
abstract：:The catalytically competent active-site structure of a true acylenzyme reaction intermediate of TEM-1 beta-lactamase formed with the kinetically specific spin-labeled substrate 6-N-(2,2,5,5-tetramethyl-1-oxypyrrolinyl-3-carboxyl)-penicillanic acid isolated under cryoenzymologic conditions has been determined by angle-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0021075
更新日期：2001-02-27 00:00:00
abstract：:Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0618262
更新日期：2007-05-01 00:00:00
abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00469a004
更新日期：1990-05-01 00:00:00
abstract：:The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00156a003
更新日期：1992-10-20 00:00:00
abstract：:A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been carried out using NMR spectroscopy. Using a randomly 15N labeled sample, a number of heteronuclear three- and two-dimensional NMR experiments have been performed, which have enabled a complete analysis of short-, mediu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00471a023
更新日期：1990-05-15 00:00:00
abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00364a007
更新日期：1986-08-12 00:00:00
abstract：:Retinol (vitamin A alcohol) is a hydrophobic compound and distributes in vivo mainly between binding proteins and cellular membranes. To better clarify the nature of the interactions of retinol with these phases which have a high affinity for it, the thermodynamic parameters of these interactions were studied. The tem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00468a014
更新日期：1990-04-24 00:00:00
abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701533j
更新日期：2008-01-22 00:00:00
abstract：:DNA-protein cross-links (DPCs) in nucleosome core particles (NCPs), the fundamental building block of chromatin, arise during times of cellular oxidative stress. These lesions are expected to be detrimental to the cell due to interference with processes like chromatin remodeling, transcription, DNA replication, and ep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700475b
更新日期：2007-09-25 00:00:00
abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00223a035
更新日期：1991-03-05 00:00:00
abstract：:The l-lyxonate dehydratase (LyxD) in vitro enzymatic activity and in vivo metabolic function were assigned to members of an isofunctional family within the mandelate racemase (MR) subgroup of the enolase superfamily. This study combined in vitro and in vivo data to confirm that the dehydration of l-lyxonate is the bio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5004298
更新日期：2014-05-27 00:00:00
