Abstract：
:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typically appear as a single event, and therefore, induced folding is extremely difficult to characterize experimentally. In this perspective, the interaction between the disordered C-terminal domain of the measles virus nucleoprotein NTAIL and the folded X domain of the viral phosphoprotein (XD) is particularly interesting because the inherent complexity of the observed kinetics allows characterization of the binding and folding steps individually. Here we present a detailed structural description of the folding and binding events occurring in the recognition between NTAIL and XD. This result was achieved by measuring the effect of single-amino acid substitutions in NTAIL on the reaction mechanism. Analysis of the experimental data allowed us (i) to identify the key residues involved in the initial recognition between the two molecules and (ii) to depict the general features of the folding pathway of NTAIL. Furthermore, an analysis of the changes in stability obtained for the whole set of variants highlights how the sequence of this IDP has not been selected during evolution to fold efficiently. This feature might be a consequence of the weakly funneled nature of the energy landscape of IDPs in their unbound state and represents a plausible explanation of their highly dynamic nature even in the bound state, typically defined as "fuzziness".
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bonetti D,Troilo F,Toto A,Brunori M,Longhi S,Gianni Sdoi
10.1021/acs.biochem.7b00350subject
Has Abstractpub_date
2017-07-25 00:00:00pages
3780-3786issue
29eissn
0006-2960issn
1520-4995journal_volume
56pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Vibrational circular dichroism (VCD) and Fourier transform IR (FTIR) were measured for a series of short alanine-based peptides having the general formula Ac-(AAKAA)n-GY-NH2 (n = 1-4) from 5 to 50 degrees C in D2O and at room temperature in both TFE and H2O. In both of these latter solvents, the dominant structural fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971460g
更新日期：1997-12-09 00:00:00
abstract：:A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state k...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00581a013
更新日期：1979-07-10 00:00:00
abstract：:We have used [(1)H-(15)N]-HSQC NMR to investigate the structural changes that occur in both serpin and proteinase in forming the kinetically trapped covalent protein-protein complex that is the basis for serpin inhibition of serine proteinases. By alternately using (15)N-alanine specifically-labeled alpha(1)-proteinas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010100x
更新日期：2001-05-29 00:00:00
abstract：:Three modified hammerhead ribozyme/substrate complexes have been prepared in which individual uridine O2-carbonyls have been eliminated. The modified complexes were chemically synthesized with the substitution of a single 2-pyridone (2P) base analogue for residues U4, U7, and U16.1. Steady-state kinetic analyses indic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982977a
更新日期：1999-06-15 00:00:00
abstract：:Phosphinothricyl-alanyl-alanine (Pt tripeptide (Ptt), bialaphos) is a metabolite produced by Streptomyces viridochromogenes and Streptomyces hygroscopicus. It contains the unique phosphinoamino acid phosphinothricin (Pt), which after cleavage from Ptt is active as an inhibitor of glutamine synthetase. We have isolated...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9719410
更新日期：1998-02-10 00:00:00
abstract：:Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00337a032
更新日期：1985-07-30 00:00:00
abstract：:(1) The rate of ATP synthesis during NADH-driven aerobic respiration has been measured in plasma membrane vesicles from Paracoccus denitrificans as a function of the concentration of the substrates, ADP and inorganic phosphate (Pi). In both cases, the response of the reaction to changes in the degree of saturation of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00498a013
更新日期：1990-11-20 00:00:00
abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972303k
更新日期：1998-02-24 00:00:00
abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0114372
更新日期：2001-11-27 00:00:00
abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010525j
更新日期：2001-09-11 00:00:00
abstract：:A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700596s
更新日期：2007-07-17 00:00:00
abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a026
更新日期：1988-07-12 00:00:00
abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00380a018
更新日期：1987-03-24 00:00:00
abstract：:There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062177x
更新日期：2007-02-20 00:00:00
abstract：:The nitrite anion is known to oxidize and degrade hemoglobin (Hb). Recent literature reports suggest a nitrite reductase activity for Hb, converting nitrite into nitric oxide. Surprisingly, no structural information about Hb-nitrite interactions has been reported. We have determined the crystal structure of the ferric...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801015c
更新日期：2008-08-12 00:00:00
abstract：:The ratio of the second-order rate constants for reduction of dihydroxyacetone phosphate (DHAP) and of the neutral truncated substrate glycolaldehyde (GLY) by glycerol 3-phosphate dehydrogenase (NAD (+), GPDH) saturated with NADH is (1.0 x 10 (6) M (-1) s (-1))/(8.7 x 10 (-3) M (-1) s (-1)) = 1.1 x 10 (8), which was u...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8001743
更新日期：2008-04-22 00:00:00
abstract：:The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9009743
更新日期：2009-11-03 00:00:00
abstract：:Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00626a025
更新日期：1977-04-05 00:00:00
abstract：:It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0006767
更新日期：2000-09-12 00:00:00
abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982401l
更新日期：1999-03-23 00:00:00
abstract：:The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a002
更新日期：1984-06-05 00:00:00
abstract：:We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951314f
更新日期：1996-03-05 00:00:00
abstract：:We have used site-directed mutagenesis to alter the [Fe-S] cluster composition of Escherichia coli dimethyl sulfoxide (DMSO) reductase (DmsABC). The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [Cammack, R., & Weiner, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00098a003
更新日期：1991-08-27 00:00:00
abstract：:Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：
更新日期：2001-08-28 00:00:00
abstract：:Characterization of the complete gene sequence encoding the alpha-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl hydrolase family 27 [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. This family, together with the family 36 alpha-galactosidases, forms g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0008074
更新日期：2000-08-15 00:00:00
abstract：:Galactosyltransferase (UDPgalactose:glycoprotein galactosyltransferase EC 2.4.1.22) was isolated from swine mesentary lymhromatography on Sepharose 4B colums containing covalently bound p-aminophenyl-beta-D-N-acetylglucosamine. The homogenous enzyme showed a single band on disc gel electrophoresis and had a specific a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00668a009
更新日期：1976-11-16 00:00:00
abstract：:Lytic polysaccharide monooxygenases (LPMOs) are ubiquitous oxidoreductases, facilitating the degradation of polymeric carbohydrates in biomass. Cellobiose dehydrogenase (CDH) is a biologically relevant electron donor in this process, with the electrons resulting from cellobiose oxidation being shuttled from the CDH de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01178
更新日期：2019-03-05 00:00:00
abstract：:Several strains of Sphingobium chlorophenolicum have been isolated from soil that was heavily contaminated with pentachlorophenol (PCP), a toxic pesticide introduced in the 1930s. S. chlorophenolicum appears to have assembled a poorly functioning pathway for degradation of PCP by patching enzymes recruited via two ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300261p
更新日期：2012-05-08 00:00:00
abstract：:Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue wit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960482k
更新日期：1996-06-18 00:00:00
abstract：:Prolyl hydroxylase is a glycoprotein containing two nonidentical subunits, alpha and beta. The alpha subunit of prolyl hydroxylase isolated from 13-day-old chick embryos contains a single high mannose oligosaccharide having seven mannosyl residues. Two forms of alpha subunit have been shown to exist in enzyme purified...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00342a041
更新日期：1985-10-08 00:00:00
