Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. Copurification of HPr and alpha 1-6 glucan.

abstract：:The solution structure of the complex formed between an oligodeoxynucleotide containing a two-base bulge (5'-CCATCGTCTACCTTTGGTAGGATGG) and SCA-alpha2, a designed spirocyclic helical molecule, has been elucidated. SCA-alpha2, a close mimic of the metabolite, NCSi-gb, of the DNA bulge-specific enediyne antibiotic neoca...

journal_title：Biochemistry

authors： Zhang N,Lin Y,Xiao Z,Jones GB,Goldberg IH

更新日期：2007-04-24 00:00:00

abstract：:11Beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) catalyzes the conversion of 11-dehydrocorticosterone to its active form corticosterone in rodents (or cortisone to cortisol in humans). The reductive reaction of the 11-keto to 11-hydroxyl is the pivotal switch in the activation of glucocorticoids. An excess of ...

journal_title：Biochemistry

authors： Zhang J,Osslund TD,Plant MH,Clogston CL,Nybo RE,Xiong F,Delaney JM,Jordan SR

更新日期：2005-05-10 00:00:00

abstract：:Raman spectra of phycocyanobilin, phycocyanin, and allophycocyanin were obtained at resonance with their visible and near-UV transitions. These spectra were empirically assigned with the help of 14N- and 15N-isotopic substitutions and comparisons with resonance Raman spectra of phycoerythrin. These results confirm the...

journal_title：Biochemistry

authors： Szalontai B,Gombos Z,Csizmadia V,Bagyinka C,Lutz M

更新日期：1994-10-04 00:00:00

abstract：:The formation of ternary complexes involving serine proteases, borate, and an alcohol has important implications for understanding the physiological actions of borate and for the development of tight binding inhibitors for this class of enzymes. Recent studies of a related enzyme, gamma-glutamyl transpeptidase, which ...

journal_title：Biochemistry

authors： London RE,Gabel SA

更新日期：2002-05-14 00:00:00

abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

journal_title：Biochemistry

authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

更新日期：2005-04-26 00:00:00

abstract：:Uncontrolled proteolysis due to cathepsin G (cat G) may cause severe pathological disorders. Cat G is inhibited by alpha 1-antichymotrypsin (ACT) and alpha 1-proteinase inhibitor (alpha 1PI), two members of the serpin superfamily of proteins. To see whether these two inhibitors play a physiological proteolysis-prevent...

journal_title：Biochemistry

authors： Duranton J,Adam C,Bieth JG

更新日期：1998-08-11 00:00:00

abstract：:Flavocytochrome b2 from Saccharomyces cerevisiae couples L-lactate dehydrogenation to cytochrome c reduction. At 25 degrees C, 0.10 M ionic strength, and saturating L-lactate concentration, the turnover rate is 207 s-1 [per cytochrome c reduced; Miles, C. S., Rouviere, N., Lederer, F., Mathews, F. S., Reid, G. A., Bla...

journal_title：Biochemistry

authors： Daff S,Sharp RE,Short DM,Bell C,White P,Manson FD,Reid GA,Chapman SK

更新日期：1996-05-21 00:00:00

abstract：:In the ATP-dependent carboxylation of biotin that is catalyzed by most biotin-dependent carboxylases, a fundamental mechanistic question is whether the ATP activates bicarbonate (via the formation of carboxyphosphate as an intermediate) or whether the ATP activates biotin (via the formation of O-phosphobiotin). We hav...

journal_title：Biochemistry

authors： Ogita T,Knowles JR

更新日期：1988-10-18 00:00:00

abstract：:The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...

journal_title：Biochemistry

authors： Kelly SJ,Butler LG

更新日期：1977-03-22 00:00:00

abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

journal_title：Biochemistry

authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

更新日期：2012-01-17 00:00:00

abstract：:Separation of the gradient-purified gastric microsome into two membrane subfractions of distinct enzymatic and phospholipid composition has been achieved by mild SDS (0.033% w/v) treatment followed by sucrose gradient centrifugation of the pig and rabbit gastric microsomes. While the high-density membranes had all of ...

journal_title：Biochemistry

authors： Ray TK,Das PK,Nandi J,Banerjee A,Bandopadhyay S

更新日期：1988-12-13 00:00:00

abstract：:We used two chemical modifiers, tetranitromethane (TNM) and acetic anhydride (Ac(2)O), which specifically target accessible tyrosine and lysine residues, respectively, to modify recombinant Syrian hamster PrP(90-231) [rSHaPrP(90-231)] and SHaPrP 27-30, the proteinase K-resistant core of PrP(Sc) isolated from brain of ...

journal_title：Biochemistry

authors： Gong B,Ramos A,Vázquez-Fernández E,Silva CJ,Alonso J,Liu Z,Requena JR

更新日期：2011-06-07 00:00:00

abstract：:We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz...

journal_title：Biochemistry

authors： Kitagawa H,Nakada H,Kurosaka A,Hiraiwa N,Numata Y,Fukui S,Funakoshi I,Kawasaki T,Yamashina I,Shimada I

更新日期：1989-10-31 00:00:00

abstract：:The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...

journal_title：Biochemistry

authors： Jucker FM,Phillips RM,McCallum SA,Pardi A

更新日期：2003-03-11 00:00:00

abstract：:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native ...

journal_title：Biochemistry

authors： Naito Y,Lowenstein JM

更新日期：1981-09-01 00:00:00

abstract：:The BCL-2 gene encodes a 25 kDa membrane protein that plays critical roles in the control of apoptosis. The regulation of BCL-2 gene expression is highly complex and occurs both transcriptionally and posttranscriptionally. In particular, the 5' upstream region of BCL-2 contains a number of elements that control its ex...

journal_title：Biochemistry

authors： Shahid R,Bugaut A,Balasubramanian S

更新日期：2010-09-28 00:00:00

abstract：:The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha...

journal_title：Biochemistry

authors： Rusnak F,Faraci WS,Walsh CT

更新日期：1989-08-22 00:00:00

abstract：:Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...

journal_title：Biochemistry

authors： Khopde S,Biswas EE,Biswas SB

更新日期：2002-12-17 00:00:00

abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...

journal_title：Biochemistry

authors： Askerka M,Vinyard DJ,Brudvig GW,Batista VS

更新日期：2015-09-29 00:00:00

abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

journal_title：Biochemistry

authors： Fede A,Labhardt A,Bannwarth W,Leupin W

更新日期：1991-12-03 00:00:00

abstract：:(E)-4-(4-Chlorophenyl)-2-oxo-3-butenoic acid (CPB) was found to irreversibly inactivate brewers' yeast pyruvate decarboxylase (PDC, EC 4.1.1.1) in a biphasic, sigmoidal manner, as is found for the kinetic behavior of substrate. An expression was derived for two-site irreversible inhibition of allosteric enzymes, and t...

journal_title：Biochemistry

authors： Kuo DJ,Jordan F

更新日期：1983-08-02 00:00:00

abstract：:Bean-deleterious Pseudomonas A214 produced the extracellular yellow-green, fluorescent siderophore [microbial iron(III) transport agent] pseudobactin A214 under iron-limiting conditions. Pseudobactin A214 has a molecular formula of C46H64N13O22 and a molecular mass of 1151 g/mol. Pseudobactin A214 contained an N-block...

journal_title：Biochemistry

authors： Buyer JS,Wright JM,Leong J

更新日期：1986-09-23 00:00:00

abstract：:Most RNA molecules that are endowed with catalytic activity function in the form of ribonucleoproteins within cells. These complexes are frequently large, poorly defined, and difficult to study. As a model system to study biological catalysis by ribonucleoproteins, we have modified the hairpin ribozyme by inserting an...

journal_title：Biochemistry

authors： Sargueil B,Pecchia DB,Burke JM

更新日期：1995-06-13 00:00:00

abstract：:4-Oxalocrotonate tautomerase (4-OT) and YwhB, a 4-OT homologue found in Bacillus subtilis, exhibit a low level hydratase activity that converts trans-3-haloacrylates to acetaldehyde, presumably through a malonate semialdehyde intermediate. The mechanism for the initial transformation of the 3-haloacrylate to malonate ...

journal_title：Biochemistry

authors： Wang SC,Johnson WH Jr,Czerwinski RM,Whitman CP

更新日期：2004-01-27 00:00:00

abstract：:The electric organ of the ray, Torpedo ocellata, can serve as a source for both the acetylcholine (ACh) receptor and its ionic channel. The two entities were identified by their specific binding of [3H]ACh and [3H]perhydrohistrionicotoxin ([3H]H12-HTX), respectively. Binding of [3H]H12-HTX was inhibited by certain dru...

journal_title：Biochemistry

authors： Eldefrawi ME,Eldefrawi AT,Mansour NA,Daly JW,Witkop B,Albuquerque EX

更新日期：1978-12-12 00:00:00

abstract：:Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. Amino acid sequence alignments of the proteins from various sources suggested that several unusual di...

journal_title：Biochemistry

authors： Firestine SM,Davisson VJ

更新日期：1994-10-04 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:Human Monocyte Chemotactic Protein (MCP)-2 has originally been isolated from stimulated osteosarcoma cells as a chemokine coproduced with MCP-1 and MCP-3. Here, a 5'-end extended MCP-2 cDNA was cloned from a human testis cDNA library. It encoded a 76 residue MCP-2 protein, but differed from the reported bone marrow-de...

journal_title：Biochemistry

authors： Van Coillie E,Proost P,Van Aelst I,Struyf S,Polfliet M,De Meester I,Harvey DJ,Van Damme J,Opdenakker G

更新日期：1998-09-08 00:00:00

abstract：:Enterokinase has been isolated from the contents of bovine duodena and purified 1200-fold in 41% yield. The isolation procedure employed DEAE chromatography, affinity chromatography on immobilized p-aminobenzamidine, and gel filtration. The resultant pure enzyme exhibits a single band on sodium dodecyl sulfate gel ele...

journal_title：Biochemistry

authors： Anderson LE,Walsh KA,Neurath H

更新日期：1977-07-26 00:00:00

abstract：:The DNA strand cleavage reaction catalyzed by endonuclease III from Escherichia coli (endo III) on the 3'-side of aldehyde abasic sites proceeds by a syn beta-elimination involving abstraction of the 2'-pro-S proton and formation of a trans alpha,beta-unsaturated aldose product; we previously reported the same stereoc...

journal_title：Biochemistry

authors： Mazumder A,Gerlt JA,Absalon MJ,Stubbe J,Cunningham RP,Withka J,Bolton PH

更新日期：1991-01-29 00:00:00