NH3 Binding to the S2 State of the O2-Evolving Complex of Photosystem II: Analogue to H2O Binding during the S2 → S3 Transition.

abstract：:Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide are deduced by using high-resolution 1H NMR techniques. The nonexchangeable and imino proton reson...

journal_title：Biochemistry

authors： Lee M,Chang DK,Hartley JA,Pon RT,Krowicki K,Lown JW

更新日期：1988-01-12 00:00:00

abstract：:We have determined the fidelity of in vitro DNA synthesis catalyzed at high temperature by the DNA polymerase from the thermophilic bacterium Thermus aquaticus. Using a DNA substrate that contains a 3'-OH terminal mismatch, we demonstrate that the purified polymerase lacks detectable exonucleolytic proofreading activi...

journal_title：Biochemistry

authors： Tindall KR,Kunkel TA

更新日期：1988-08-09 00:00:00

abstract：:Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...

journal_title：Biochemistry

authors： Takigami T,Takeuchi F,Nakagawa M,Hase T,Tsubaki M

更新日期：2003-07-15 00:00:00

abstract：:Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...

journal_title：Biochemistry

authors： Colon W,Kelly JW

更新日期：1992-09-15 00:00:00

abstract：:An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...

journal_title：Biochemistry

authors： Cabezas A,Pinto RM,Fraiz F,Canales J,González-Santiago S,Cameselle JC

更新日期：2001-11-13 00:00:00

abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...

journal_title：Biochemistry

authors： Smith PA,Jackson V,Chalkley R

更新日期：1984-03-27 00:00:00

abstract：:Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...

journal_title：Biochemistry

authors： Yan Q,Murphy-Ullrich JE,Song Y

更新日期：2011-02-01 00:00:00

abstract：:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...

journal_title：Biochemistry

authors： Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JM

更新日期：1993-12-14 00:00:00

abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...

journal_title：Biochemistry

authors： Nicely NI,Parsonage D,Paige C,Newton GL,Fahey RC,Leonardi R,Jackowski S,Mallett TC,Claiborne A

更新日期：2007-03-20 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) kinase is activated by a variety of mutations in human cancers. R776H is one such recurrent mutation (R752H in another numbering system) in the αC-β4 loop of the tyrosine kinase domain that activates EGFR in the absence of the activating EGF ligand. However, the mechanistic ...

journal_title：Biochemistry

authors： Ruan Z,Kannan N

更新日期：2015-07-14 00:00:00

abstract：:In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52-58]. Recently, we found that subcloned PC12h cells accumulated another un...

journal_title：Biochemistry

authors： Ariga T,Yu RK,Scarsdale JN,Suzuki M,Kuroda Y,Kitagawa H,Miyatake T

更新日期：1988-07-12 00:00:00

abstract：:The activation of coagulation factor X by tissue factor (TF) and coagulation factor VIIa (VIIa) on a phospholipid surface is thought to be the key step in the initiation of blood coagulation. In this reaction, the product, fXa, is transiently and reversibly bound to the TF-VIIa enzyme complex. This in effect leads to ...

journal_title：Biochemistry

authors： Hathcock J,Rusinova E,Vaananen H,Nemerson Y

更新日期：2007-05-22 00:00:00

abstract：:Phosphatidylinositol-specific phospholipase C (PLC) enzymes catalyze the hydrolysis of phosphatidylinositol 4,5 bisphosphate in a two step reaction that involves a cyclic intermediate. The PLCbetafamily are activated by both the alpha and betagamma subunits of heterotrimeric G proteins. To determine which catalytic st...

journal_title：Biochemistry

authors： Feng J,Roberts MF,Drin G,Scarlata S

更新日期：2005-02-22 00:00:00

abstract：:The reaction of Pseudomonas fluorescens kynureninase with L-kynurenine and L-alanine has been examined using rapid-scanning stopped-flow spectrophotometry. Mixing kynureninase with 0.5 mM L-kynurenine results in formation of a quinonoid intermediate, with lambdamax = 494 nm, within the dead time (ca. 2 ms) of the stop...

journal_title：Biochemistry

authors： Phillips RS,Sundararaju B,Koushik SV

更新日期：1998-06-16 00:00:00

abstract：:Protein amyloid fibrils are originally identified as pathological entities in a variety of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Recent studies have revealed that amyloid fibrils also serve as functional protein assemblies to fulfill a wide range of biological functions. Decip...

journal_title：Biochemistry

authors： Li D,Liu C

更新日期：2020-02-11 00:00:00

abstract：:Despite much work, subcellular neurons of Caenorhabditis elegans have not been studied at nanometer resolution with millisecond time resolution. Nor has there been an effective way to immobilize C. elegans. Here we show that, without using anesthetic or paralyzing agents, fluorescence imaging with one-nanometer accura...

journal_title：Biochemistry

authors： Kural C,Nonet ML,Selvin PR

更新日期：2009-06-09 00:00:00

abstract：:Phospholipase A2 from Naja naja naja venom (Indian cobra) undergoes a concentration-dependent aggregation, and at an assay concentration of 1 microgram mL-1, it exists as a monomer. However, there is some evidence that the enzyme is actually active as a dimer or higher order aggregate. Previous attempts to determine t...

journal_title：Biochemistry

authors： Hazlett TL,Dennis EA

更新日期：1985-10-22 00:00:00

abstract：:Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myoc...

journal_title：Biochemistry

authors： Su X,Han X,Mancuso DJ,Abendschein DR,Gross RW

更新日期：2005-04-05 00:00:00

abstract：:Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...

journal_title：Biochemistry

authors： Momb J,Thomas PW,Breece RM,Tierney DL,Fast W

更新日期：2006-11-07 00:00:00

abstract：:The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...

journal_title：Biochemistry

authors： Quay SC,Condie CC

更新日期：1983-02-01 00:00:00

abstract：:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial se...

journal_title：Biochemistry

authors： Oakley MG,Kim PS

更新日期：1997-03-04 00:00:00

abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...

journal_title：Biochemistry

authors： Mills M,Völker J,Klump HH

更新日期：1996-10-15 00:00:00

abstract：:Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...

journal_title：Biochemistry

authors： Sutoh K,Hiratsuka T

更新日期：1988-04-19 00:00:00

abstract：:Indolamine N-methyltransferase (INMT) has been purified to an apparent homogeneity from rabbit lung, and some of its catalytic and physicochemical properties have been examined. The enzyme is a monomeric protein with a molecular weight of 31,500 +/- 1000, a molecular Stokes radius of 21.5 A, and a diffusion coefficien...

journal_title：Biochemistry

authors： Irace G,Colonna G,Camardella M,Della Pietra G,Porta R

更新日期：1982-03-16 00:00:00

abstract：:A method is presented for the rapid determination of substrate selection in a manner that is not restricted to conditions of metabolic and isotopic steady state. Competition between several substrates can be assessed directly and continuously in a single experiment, allowing the effect of interventions to be studied. ...

journal_title：Biochemistry

authors： Malloy CR,Thompson JR,Jeffrey FM,Sherry AD

更新日期：1990-07-24 00:00:00

abstract：:The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...

journal_title：Biochemistry

authors： Van Hoegaerden M,Strosberg AD

更新日期：1978-10-03 00:00:00

abstract：:We have investigated the interaction of a number of synthetic 20-residue peptides, corresponding to the HA2 N-terminus of the influenza virus hemagglutinin (X31 strain), with phospholipid vesicles and monolayers. Besides the wild-type sequence, two peptides were studied with mutations corresponding to those previously...

journal_title：Biochemistry

authors： Rafalski M,Ortiz A,Rockwell A,van Ginkel LC,Lear JD,DeGrado WF,Wilschut J

更新日期：1991-10-22 00:00:00

abstract：:The antimicrobial peptide MSI-78 serves as a model system for studying interactions of bioactive peptides with membranes. Using a series of MSI-78 peptides that incorporate l-4,4,4-trifluoroethylglycine, a small and sensitive (19)F nuclear magnetic resonance probe, we investigated how the local structure and dynamics ...

journal_title：Biochemistry

authors： Suzuki Y,Buer BC,Al-Hashimi HM,Marsh EN

更新日期：2011-07-12 00:00:00

abstract：:Fourier transform infrared spectra of light-adapted bacteriorhodopsin exhibit a band at 1618 cm(-1) that shifts to 1625 cm(-1) upon formation of the L intermediate. It is assigned to the peptide C==O of Val49 from the fact that it shifts in [1-(13)C]valine-labeled bacteriorhodopsin and appears perturbed in the Val49--...

journal_title：Biochemistry

authors： Yamazaki Y,Tuzi S,Saitô H,Kandori H,Needleman R,Lanyi JK,Maeda A

更新日期：1996-04-02 00:00:00

abstract：:The active form of the animal fatty acid synthase (FAS) is a dimer of identical multifunctional polypeptides, each containing seven discrete functional domains, that cooperate to form two centers for palmitate synthesis. To assess the importance of domain cooperation across the subunit interface in the reaction mechan...

journal_title：Biochemistry

authors： Joshi AK,Witkowski A,Smith S

更新日期：1998-02-24 00:00:00