Two-stage maturation process for newly replicated chromatin.

abstract：:Many important human pathogens rely on one or more type three secretion systems (T3SSs) to inject bacterial effector proteins directly into the host cell cytoplasm. Secretion of protein through the needlelike type three secretion apparatus (T3SA) is essential for pathogen virulence and relies on a highly conserved ATP...

journal_title：Biochemistry

authors： Case HB,Mattock DS,Dickenson NE

更新日期：2018-12-18 00:00:00

abstract：:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage pr...

journal_title：Biochemistry

authors： Burger RM,Horwitz SB,Peisach J

更新日期：1985-07-02 00:00:00

abstract：:The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in c...

journal_title：Biochemistry

authors： Agarwal K,Baek KH,Jeon CJ,Miyamoto K,Ueno A,Yoon HS

更新日期：1991-08-06 00:00:00

abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...

journal_title：Biochemistry

authors： Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

更新日期：2007-06-19 00:00:00

abstract：:Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...

journal_title：Biochemistry

authors： Sugiki T,Furuita K,Fujiwara T,Kojima C

更新日期：2018-07-03 00:00:00

abstract：:Complementary DNA (cDNA) clones encoding bovine testicular 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) have been isolated from a bovine testicular lambda gt11 library using polyclonal antibodies against 20 alpha-HSD and DNA probe hybridization. Nucleotide sequencing of three independently isolated clones was ...

journal_title：Biochemistry

authors： Warren JC,Murdock GL,Ma Y,Goodman SR,Zimmer WE

更新日期：1993-02-16 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:Pluronic block copolymers (triblock copolymers of poly(ethylene oxide) and poly(propylene oxide)) exhibit a chemosensitizing effect on multidrug resistant cell lines. Changes in membrane permeability are hypothesized to be responsible because inhibition of drug transport mediated by both the multidrug-resistance-assoc...

journal_title：Biochemistry

authors： Krylova OO,Pohl P

更新日期：2004-03-30 00:00:00

abstract：:CC chemokine receptor 5 (CCR5) is the receptor for several inflammatory chemokines and is a coreceptor for HIV-1. Posttranslational sulfation of tyrosines in the N-terminal regions of chemokine receptors has been shown to be important in the binding affinity for chemokine ligands. In addition, sulfation of CCR5 is cru...

journal_title：Biochemistry

authors： Jen CH,Moore KL,Leary JA

更新日期：2009-06-16 00:00:00

abstract：:The pyridoxal 5'-phosphate (PLP)-dependent cystathionine beta-lyase (CBL) was previously found to be inhibited by the natural toxins rhizobitoxine and l-aminoethoxyvinylglycine (AVG). The present study characterizes the interaction of Escherichia coli CBL with AVG and methoxyvinylglycine (MVG) by a combination of kine...

journal_title：Biochemistry

authors： Clausen T,Huber R,Messerschmidt A,Pohlenz HD,Laber B

更新日期：1997-10-14 00:00:00

abstract：:The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...

journal_title：Biochemistry

authors： Juszczak LJ,Desamero RZ

更新日期：2009-03-31 00:00:00

abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

journal_title：Biochemistry

authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

更新日期：1999-10-12 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:Incubation of bovine brain derived acidic fibroblast growth factor (aFGF) with bovine or human thrombin, 0.5 NIH unit/mL, for 24 h at 37 degrees C results in cleavage of the mitogen, generating a 14-kilodalton fragment which has significantly reduced affinity for immobilized heparin as compared to aFGF, and is at leas...

journal_title：Biochemistry

authors： Lobb RR

更新日期：1988-04-05 00:00:00

abstract：:A hairpin structure contains two conformationally distinct domains: a double-helical stem with Watson-Crick base pairs and a single-stranded loop that connects the two arms of the stem. By extensive 1D and 2D 500-MHz 1H NMR studies in H2O and D2O, it has been demonstrated that the DNA oligomers d(CGCCGCAGC) and d(CGCC...

journal_title：Biochemistry

authors： Gupta G,Sarma MH,Sarma RH,Bald R,Engelke U,Oei SL,Gessner R,Erdmann VA

更新日期：1987-12-01 00:00:00

abstract：:The association state of the mannitol-specific enzyme II (EIIMtl) has been studied both in the purified form and embedded in the cytoplasmic membrane. Membrane fragments obtained from mannitol-grown Escherichia coli catalyze the phosphoenolpyruvate- (PEP) dependent phosphorylation of both glucose and mannitol; thus th...

journal_title：Biochemistry

authors： Roossien FF,Robillard GT

更新日期：1984-11-20 00:00:00

abstract：:Three crystal structures have been determined of active site specific substituted Cd(II) horse liver alcohol dehydrogenase and its complexes. Intensities were collected for the free, orthorhombic enzyme to 2.4-A resolution and for a triclinic binary complex with NADH to 2.7-A resolution. A ternary complex was crystall...

journal_title：Biochemistry

authors： Schneider G,Cedergren-Zeppezauer E,Knight S,Eklund H,Zeppezauer M

更新日期：1985-12-03 00:00:00

abstract：:It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...

journal_title：Biochemistry

authors： Baldisseri DM,Torchia DA,Poole LB,Gerlt JA

更新日期：1991-04-16 00:00:00

abstract：:CDPKs are a family of calcium (Ca2+)-dependent protein kinases which are defined by a carboxyl-terminal calmodulin-like domain. Mutational analysis indicates that the junction domain, which joins the kinase and calmodulin-like domains, contains an autoinhibitor. CDPK isoform AK1 from Arabidopsis was expressed in Esche...

journal_title：Biochemistry

authors： Harper JF,Huang JF,Lloyd SJ

更新日期：1994-06-14 00:00:00

abstract：:RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...

journal_title：Biochemistry

authors： Malik SS,Rich E,Viswanathan R,Cairns BR,Fischer CJ

更新日期：2011-09-20 00:00:00

abstract：:Membrane-bound phosphodiesterase 6 (PDE6) plays an important role in visual signal transduction by regulating cGMP levels in rod photoreceptor cells. Our understanding of PDE6 catalysis and structure suffers from inadequate characterization of the α and β subunit catalytic core, interactions of the core with two intri...

journal_title：Biochemistry

authors： Baker BY,Palczewski K

更新日期：2011-11-08 00:00:00

abstract：:The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide groups of Lys 24 and Gly 83. These are conserved residues in each of the consensus sequences....

journal_title：Biochemistry

authors： Lowry DF,Cool RH,Redfield AG,Parmeggiani A

更新日期：1991-11-12 00:00:00

abstract：:A series of truncated forms of His(6)-tagged gp91phox were expressed, solubilized, and purified in the presence of 30 microM FAD. The truncated gp91phox with the longest sequence in the C-terminal region (221-570) (gp91C) showed the highest activity (turnover rate, 0.92) for NADPH diaphorase in the presence of either ...

journal_title：Biochemistry

authors： Nisimoto Y,Ogawa H,Miyano K,Tamura M

更新日期：2004-07-27 00:00:00

abstract：:The antimicrobial peptide MSI-78 serves as a model system for studying interactions of bioactive peptides with membranes. Using a series of MSI-78 peptides that incorporate l-4,4,4-trifluoroethylglycine, a small and sensitive (19)F nuclear magnetic resonance probe, we investigated how the local structure and dynamics ...

journal_title：Biochemistry

authors： Suzuki Y,Buer BC,Al-Hashimi HM,Marsh EN

更新日期：2011-07-12 00:00:00

abstract：:Mitochondrial thiolase I from pig heart has been found to have at least two and possibly three reactive sulfhydryl residues at or near the active site [Izbicka-Dimitrijević, E., & Gilbert, H. F. (1982) Biochemistry 21, 6112-6118; Izbicka-Dimitrijević, E., & Gilbert, H. F. (1984) Biochemistry 23, 4318-4324]. In the nat...

journal_title：Biochemistry

authors： Izbicka E,Gilbert HF

更新日期：1984-12-18 00:00:00

abstract：:The hairpin catalytic motif in tobacco ringspot virus satellite RNA consists of two helix-loop-helix elements on two adjacent arms of a four-way helical junction. The bases essential for catalytic activity are located in the loops that are brought into proximity by a conformational change as a prerequisite for catalys...

journal_title：Biochemistry

authors： Klostermeier D,Millar DP

更新日期：2001-09-18 00:00:00

abstract：:Colibactins are genotoxic secondary metabolites whose biosynthesis is encoded in the clb gene cluster harbored by certain strains of gut commensal Escherichia coli. Using synthetic colibactin analogues, we previously provided evidence that colibactins alkylate DNA by addition of a nucleotide to an electrophilic cyclop...

journal_title：Biochemistry

authors： Xue M,Shine E,Wang W,Crawford JM,Herzon SB

更新日期：2018-11-13 00:00:00

abstract：:The fluorescence of the base analogue 2-aminopurine (2AP) was used to probe bacteriophage T4 DNA polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2AP fluorescence in DNA is quenched by 2AP interactions with neighboring bases, but ...

journal_title：Biochemistry

authors： Hariharan C,Reha-Krantz LJ

更新日期：2005-12-06 00:00:00

abstract：:The initial hydrolysis of CaATP by chloroplast coupling factor 1 was studied with the quenched-flow method. The time course of hydrolysis can be described as a first-order conversion of the enzyme to an active form followed by steady-state formation of product. The rat constant for the first-order process is independe...

journal_title：Biochemistry

authors： Leckband D,Hammes GG

更新日期：1987-04-21 00:00:00

abstract：:Mild acid hydrolysis of Hafnia alvei strain 2 lipopolysaccharide released no O-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with N-acetylneuraminic acid as the reducing terminus. In addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fra...

journal_title：Biochemistry

authors： Gamian A,Romanowska E,Dabrowski U,Dabrowski J

更新日期：1991-05-21 00:00:00