Abstract：
:The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide groups of Lys 24 and Gly 83. These are conserved residues in each of the consensus sequences. Their uncharacteristic downfield proton shifts are attributed to strong hydrogen bonds to phosphate oxygens as for resonances in N-ras-p21 [Redfield, A. G., & Papastavros, M. Z. (1990) Biochemistry 29, 3509-3514]. The Lys 24 of the EF-Tu G-domain has nearly the same proton and nitrogen shifts as the corresponding Lys 16 in p21. These results suggest that this conserved lysine has a similar structural role in proteins in this class. The tentative Gly 83 resonance has no spectral analogue in p21. A mutant protein with His 84 changed to glycine was fully 15N-labeled and the proton resonance assigned to Gly 83 shifted downfield by 0.3 ppm, thereby supporting the assignment.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lowry DF,Cool RH,Redfield AG,Parmeggiani Adoi
10.1021/bi00109a010subject
Has Abstractpub_date
1991-11-12 00:00:00pages
10872-7issue
45eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:A compound tentatively identified as O2-methyl-5-carboxymethyluridine (cm5Um) was recently isolated in this laboratory from bulk yeast transfer RNA (Gray, M. W. (1975), Can, J. Biochem. 53, 735-746). Alkaline hydrolysis of yeast tRNA releases this nucleoside as part of an alkali-stable dinucleotide, cm5Um-Ap, from whi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00659a017
更新日期：1976-07-13 00:00:00
abstract：:Determining the sequence of bond cleavages, and consequently the nature of intermediates, in enzyme-catalyzed reactions is a major goal of mechanistic enzymology. When significant primary isotope effects on V/K are observed for two different bond cleavages, both bonds may be broken in the same transition state or they...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00238a013
更新日期：1991-06-18 00:00:00
abstract：:We investigated the effects of substituting two of the four tryptophans (the "inner pair" Trp(9) and Trp(11) or the "outer pair" Trp(13) and Trp(15)) in gramicidin A (gA) channels. The conformational preferences of the doubly substituted gA analogues were assessed using circular dichroism spectroscopy and size-exclusi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200136e
更新日期：2011-06-07 00:00:00
abstract：:In protein, conformational changes are often crucial for function but not easy to observe. Two functionally relevant conformational intermediate states of photosynthetic reaction center protein (RCs) are trapped and characterized at low temperature. RCs frozen in the dark do not allow electron transfer from the reduce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002326q
更新日期：2001-03-13 00:00:00
abstract：:Earlier thermodynamic studies of the intermolecular interactions between mature oxytocin and neurophysin, and of the effects of these interactions on neurophysin folding, raised questions about the intramolecular interactions of oxytocin with neurophysin within their common precursor. To address this issue, the disulf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9912950
更新日期：1999-10-12 00:00:00
abstract：:Bax protein plays a key role in mitochondrial membrane permeabilization and cytochrome c release upon apoptosis. Our recent data have indicated that the 20-residue C-terminal peptide of Bax (BaxC-KK; VTIFVAGVLTASLTIWKKMG), when expressed intracellularly, translocates to the mitochondria and exerts lethal effect on can...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301195f
更新日期：2012-11-20 00:00:00
abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991888a
更新日期：1999-12-21 00:00:00
abstract：:The incorporation of extensively fluorinated, or fluorous, analogues of hydrophobic amino acids into proteins potentially provides the opportunity to modulate the physicochemical properties of proteins in a predictable manner. On the basis of the properties of small fluorocarbon molecules, extensively fluorinated prot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901481k
更新日期：2009-11-17 00:00:00
abstract：:We consider the effects that different lipid surfaces have upon the denaturation and subsequent formation of amyloid fibrils of bovine insulin. The adsorption and unfolding kinetics of insulin being adsorbed onto the different lipid surfaces under denaturing conditions are studied using FTIR ATR spectroscopy and are c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020525z
更新日期：2002-12-31 00:00:00
abstract：:X-ray diffraction measurements show that in contrast to the purple membrane, the bacteriorhodopsin molecules are not organized in a hexagonal lattice in the deionized blue membrane. Addition of Ca2+ restores both the purple color and the normal (63 A) hexagonal protein lattice. In the blue state, the circular dichrois...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00449a031
更新日期：1989-11-14 00:00:00
abstract：:Hydrogen exchange of the individual amide protons of alanine-90 (F5), glutamine-91 (F6), serine-92 (F7), and histidine-93 (F8) residues in cyanometmyoglobin of sperm whale has been studied by 1H nuclear magnetic resonance spectroscopy at 360 MHz. The amide proton resonance of F5, F6, and F7 have been assigned by use o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00347a024
更新日期：1985-12-17 00:00:00
abstract：:Near-infrared FT-Raman spectroscopy can be used to measure the vibrations of the bacteriorhodopsin (bR) chromophore without the disadvantage of conventional visible resonance Raman spectroscopy, where the visible excitation drives the bR photoreactions. We utilized this technique to investigate the light-dark adaptati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00060a020
更新日期：1993-03-09 00:00:00
abstract：:The fusion of different protein domains via peptide linkers is a powerful, modular approach to obtain proteins with new functions. A detailed understanding of the conformational behavior of peptide linkers is important for applications such as fluorescence resonance energy transfer (FRET)-based sensor proteins and mul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061288t
更新日期：2006-11-07 00:00:00
abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) is activated by Cr2+ as the sole activator under anaerobic conditions. PEPCK was modified with Cr3+, starting with either Cr2+ or Cr3+. Cr3+ has the distinct advantage of being a paramagnetic cation that could serve as a paramagnetic probe. Activators Mn2+, Mg2+,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9807299
更新日期：1998-06-02 00:00:00
abstract：:The effect of Ca2+ binding on the circular dichroism (CD) and 270-MHz proton nuclear magnetic resonance (NMR) spectra of brain-specific S-100b calcium binding protein has been examined at two pH values, 8.5 and 7.5. At pH 8.5, S-100b protein binds two Ca2+ per monomer with Kd values of 6 x 10(-5) and 2 x 10(-4) M, whe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00276a033
更新日期：1983-03-29 00:00:00
abstract：:Purified human von Willebrand factor (vWF) was digested with Staphylococcus aureus V-8 protease, and specific domains interacting with platelets were isolated and characterized. Amino acid sequence analysis and sodium dodecyl sulfate gel electrophoresis demonstrated that the digestion proceeded primarily by a single c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00359a013
更新日期：1986-06-03 00:00:00
abstract：:Adenosine 5'-phosphosulfate (APS) kinase catalyzes the second reaction in the two-step conversion of inorganic sulfate to 3'-phosphoadenosine 5'-phosphosulfate (PAPS). This report presents the 2.0 A resolution crystal structure of ligand-free APS kinase from the filamentous fungus, Penicillium chrysogenum. The enzyme ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9924157
更新日期：2000-02-22 00:00:00
abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00272
更新日期：2015-07-21 00:00:00
abstract：:Thiol dioxygenases are mononuclear non-heme FeII-dependent metalloenzymes that initiate the oxidative catabolism of thiol-containing substrates to their respective sulfinates. Cysteine dioxygenase (CDO), the best characterized mammalian thiol dioxygenase, contains a three-histidine (3-His) coordination environment rat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00267
更新日期：2020-07-07 00:00:00
abstract：:Immunoglobulins fragments are composed of globular domains linked by extended polypeptide segments. The molecular flexibility inherent in this arrangement allows for significant potential differences between structures observed in the crystalline state and those attained in solution. Small-angle neutron scattering mea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00541a011
更新日期：1982-06-08 00:00:00
abstract：:Two-dimensional (2D) proton magnetic resonance techniques used in conjunction with laser photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy have been applied to studying the kringle 4 domain from human plasminogen at 360 MHz. Out of 11 potential CIDNP-sensitive aromatic side chains, only 5...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00372a020
更新日期：1986-12-02 00:00:00
abstract：:Malate dehydrogenase occurs in virtually all eucaryotic cells in mitochondrial and cytoplasmic forms, both of which are composed of two identical subunits. The reactivation of the mitochondrial isoenzyme has been the subject of previous studies [Jaenicke, R., Rudolph, R., & Heider, I. (1979) Biochemistry 18, 1217-1223...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00355a033
更新日期：1986-04-08 00:00:00
abstract：:Infrared spectroscopy has been used to monitor residual ordered structure in the denatured state of wild-type and two mutants of iso-1-cytochrome c. The technique used involves a careful digital subtraction procedure that removes spectral contributions from buffer, water vapor, and the denaturant guanidine hydrochlori...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00175a008
更新日期：1994-03-08 00:00:00
abstract：:To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990600e
更新日期：1999-06-15 00:00:00
abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a033
更新日期：1977-11-29 00:00:00
abstract：:Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra- and extracellular Cs+, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a003
更新日期：1988-05-17 00:00:00
abstract：:Manganese superoxide dismutase (MnSOD) cycles between the Mn(II) and Mn(III) states during the catalyzed disproportionation of O(2)(*-), a catalysis that is limited at micromolar levels of superoxide by a peroxide-inhibited complex with the metal. We have investigated the role in catalysis and inhibition of the conser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011047f
更新日期：2001-10-09 00:00:00
abstract：:Recent studies of DNA hairpin loops have shown considerable dependence of the stability on the sequence of the loop [Senior, M., Jones, R. A., & Breslauer, K. J. (1988a) Proc. Natl. Acad. Sci. U.S.A. 85, 6242-6246; Xodo, L. E., Manzini, G., Quadrifoglio, F., van der Marel, G., & van Boom, J. H. (1989) Biochimie 71, 79...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00181a026
更新日期：1994-04-19 00:00:00
abstract：:We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micromete...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200549x
更新日期：2011-05-31 00:00:00
abstract：:The reduced hydroxylase component (MMOH) of soluble methane monooxygenase (MMO) from Methylosinus trichosporium OB3b reacts with O2 and CH4 to produce CH3OH and H2O in a single-turnover reaction. Transient kinetic analysis of this reaction has revealed at least five and probably six intermediates during the turnover [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960596w
更新日期：1996-08-06 00:00:00
