Allosteric interactions of DNA and nucleotides with S. cerevisiae RSC.

abstract：:MenB, the 1,4-dihydroxy-2-naphthoyl-CoA synthase from the bacterial menaquinone biosynthesis pathway, catalyzes an intramolecular Claisen condensation (Dieckmann reaction) in which the electrophile is an unactivated carboxylic acid. Mechanistic studies on this crotonase family member have been hindered by partial acti...

journal_title：Biochemistry

authors： Li HJ,Li X,Liu N,Zhang H,Truglio JJ,Mishra S,Kisker C,Garcia-Diaz M,Tonge PJ

更新日期：2011-11-08 00:00:00

abstract：:Femtosecond spectroscopy was performed on CO-liganded (fully reduced and mixed-valence states) and O(2)-liganded quinol oxidase bd from Escherichia coli. Substantial polarization effects, unprecedented for optical studies of heme proteins, were observed in the CO photodissociation spectra, implying interactions betwee...

journal_title：Biochemistry

authors： Borisov VB,Liebl U,Rappaport F,Martin JL,Zhang J,Gennis RB,Konstantinov AA,Vos MH

更新日期：2002-02-05 00:00:00

abstract：:A kinetic folding intermediate of hen lysozyme is shown to form in a nucleation/growth type of mechanism. Under native solvent conditions, a nucleated state is formed slowly during refolding (tau = 14 +/- 1 ms at 0 M GdmCl) and is rapidly converted to the folding intermediate (tau = 300 +/- 150 micros at 0 M GdmCl). U...

journal_title：Biochemistry

authors： Kiefhaber T,Bachmann A,Wildegger G,Wagner C

更新日期：1997-04-29 00:00:00

abstract：:Heme-derived linear tetrapyrroles (phytobilins) in phycobiliproteins and phytochromes perform critical light-harvesting and light-sensing roles in oxygenic photosynthetic organisms. A key enzyme in their biogenesis, phycocyanobilin:ferredoxin oxidoreductase (PcyA), catalyzes the overall four-electron reduction of bili...

journal_title：Biochemistry

authors： Kohler AC,Gae DD,Richley MA,Stoll S,Gunn A,Lim S,Martin SS,Doukov TI,Britt RD,Ames JB,Lagarias JC,Fisher AJ

更新日期：2010-07-27 00:00:00

abstract：:The C2A domain of synaptotagmin 7 (Syt7) is a Ca(2+) and membrane binding module that docks and inserts into cellular membranes in response to elevated intracellular Ca(2+) concentrations. Like other C2 domains, Syt7 C2A binds Ca(2+) and membranes primarily through three loop regions; however, it docks at Ca(2+) conce...

journal_title：Biochemistry

authors： Chon NL,Osterberg JR,Henderson J,Khan HM,Reuter N,Knight JD,Lin H

更新日期：2015-09-22 00:00:00

abstract：:Cytochrome c-553 from Desulfovibrio vulgaris exhibits a highly exposed heme and an unusually low reduction potential with respect to other c-type cytochromes. Solvent heme exposure has been indicated as one of the most important factors in modulating the midpoint potential of the redox center. To test this hypothesis,...

journal_title：Biochemistry

authors： Fantuzzi A,Sadeghi S,Valetti F,Rossi GL,Gilardi G

更新日期：2002-07-09 00:00:00

abstract：:A combined experimental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase reaction is described. The experimental studies focus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed in these reactions is an intrinsic isotope effect resul...

journal_title：Biochemistry

authors： Francisco WA,Abu-Soud HM,DelMonte AJ,Singleton DA,Baldwin TO,Raushel FM

更新日期：1998-02-24 00:00:00

abstract：:Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...

journal_title：Biochemistry

authors： Wang H,Shu Q,Frieden C,Gross ML

更新日期：2017-06-13 00:00:00

abstract：:A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...

journal_title：Biochemistry

authors： Volwerk JJ,Mrsny RJ,Patapoff TW,Jost PC,Griffith OH

更新日期：1987-01-27 00:00:00

abstract：:Site-directed mutants were prepared of four consecutive and highly conserved residues (His-411, Asp-412, Thr-413, Tyr-414) of an extramembrane loop that connects putative transmembrane helices IX and X of subunit I of Rhodobacter sphaeroides cytochrome c oxidase. The modified enzymes were purified and analyzed by opti...

journal_title：Biochemistry

authors： Hosler JP,Shapleigh JP,Tecklenburg MJ,Thomas JW,Kim Y,Espe M,Fetter J,Babcock GT,Alben JO,Gennis RB

更新日期：1994-02-08 00:00:00

abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...

journal_title：Biochemistry

authors： Parr RD,Martin GG,Hostetler HA,Schroeder ME,Mir KD,Kier AB,Ball JM,Schroeder F

更新日期：2007-07-17 00:00:00

abstract：:The two flavodoxins (YkuN and YkuP) from Bacillus subtilis have been cloned, overexpressed in Escherichia coli and purified. DNA sequencing, mass spectrometry, and flavin-binding properties showed that both YkuN and YkuP were typical short-chain flavodoxins (158 and 151 amino acids, respectively) and that an error in ...

journal_title：Biochemistry

authors： Lawson RJ,von Wachenfeldt C,Haq I,Perkins J,Munro AW

更新日期：2004-10-05 00:00:00

abstract：:Full-length protein tyrosine phosphatase 1C (PTP1C), the catalytic domain of PTP1C (delta PTP1C), and the N-terminal SH2 domain truncated PTP1C (delta NPTP1C) were overexpressed in Escherichia coli and purified to near homogeneity. Various phosphorylated states of the synthetic phosphotyrosyl peptide TRDIYETDYYRK (IRP...

journal_title：Biochemistry

authors： Townley R,Shen SH,Banville D,Ramachandran C

更新日期：1993-12-14 00:00:00

abstract：:The mechanism of inactivation of human leukocyte elastase (HLE) by the chloromethyl ketone MeOSuc-Ala-Ala-Pro-Val-CH2Cl was investigated. The dependence of the first-order rate constant for inactivation on concentration of chloromethyl ketone is hyperbolic and suggests formation of a reversible "Michaelis complex" pri...

journal_title：Biochemistry

authors： Stein RL,Trainor DA

更新日期：1986-09-23 00:00:00

abstract：:The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...

journal_title：Biochemistry

authors： Ha-Duong NT,Eid C,Hémadi M,El Hage Chahine JM

更新日期：2010-12-07 00:00:00

abstract：:1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...

journal_title：Biochemistry

authors： Chung Y,Jue T

更新日期：1992-11-17 00:00:00

abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...

journal_title：Biochemistry

authors： Slijper M,Boelens R,Davis AL,Konings RN,van der Marel GA,van Boom JH,Kaptein R

更新日期：1997-01-07 00:00:00

abstract：:All-trans-retinol is the precursor for all-trans-retinoic acid, the activating ligand for nuclear transcription factors retinoic acid receptors. In the cytosol of various cells, most retinol exists in a bound form, complexed with cellular retinol binding protein type I (holo-CRBP). Whether retinoic acid is produced fr...

journal_title：Biochemistry

authors： Lapshina EA,Belyaeva OV,Chumakova OV,Kedishvili NY

更新日期：2003-01-28 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

journal_title：Biochemistry

authors： Ronin C,Papandreou MJ,Canonne C,Weintraub BD

更新日期：1987-09-08 00:00:00

abstract：:Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...

journal_title：Biochemistry

authors： Sewell AK,Jensen LT,Erickson JC,Palmiter RD,Winge DR

更新日期：1995-04-11 00:00:00

abstract：:We have used [(1)H-(15)N]-HSQC NMR to investigate the structural changes that occur in both serpin and proteinase in forming the kinetically trapped covalent protein-protein complex that is the basis for serpin inhibition of serine proteinases. By alternately using (15)N-alanine specifically-labeled alpha(1)-proteinas...

journal_title：Biochemistry

authors： Peterson FC,Gettins PG

更新日期：2001-05-29 00:00:00

abstract：:The ileal apical and liver basolateral bile acid transporters catalyze the Na+-dependent uptake of these amphipathic molecules in the intestine and liver. They contain nine predicted helical hydrophobic sequences (H1-H9) between the exoplasmic N-glycosylated N terminus and the cytoplasmic C terminus. Previous in vitro...

journal_title：Biochemistry

authors： Mareninova O,Shin JM,Vagin O,Turdikulova S,Hallen S,Sachs G

更新日期：2005-10-25 00:00:00

abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...

journal_title：Biochemistry

authors： Osborne RL,Zhu H,Iavarone AT,Hess CR,Klinman JP

更新日期：2012-09-25 00:00:00

abstract：:Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participated in the early phase of blood coagulation. From two genomic libraries, we succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and r...

journal_title：Biochemistry

authors： Beaubien G,Rosinski-Chupin I,Mattei MG,Mbikay M,Chrétien M,Seidah NG

更新日期：1991-02-12 00:00:00

abstract：:G.U pairs occur frequently and have many important biological functions. The stability of symmetric tandem G.U motifs depends both on the adjacent Watson-Crick base pairs, e.g., 5'G > 5'C, and the sequence of the G.U pairs, i.e., 5'-UG-3' > 5'-GU-3', where an underline represents a nucleotide in a G.U pair [Wu, M., Mc...

journal_title：Biochemistry

authors： Chen X,McDowell JA,Kierzek R,Krugh TR,Turner DH

更新日期：2000-08-01 00:00:00

abstract：:Translesion DNA synthesis represents the ability of a DNA polymerase to misinsert a nucleotide opposite a DNA lesion. Previous kinetic studies of the bacteriophage T4 DNA polymerase using a series of non-natural nucleotides suggest that pi-electron density of the incoming nucleotide substantially contributes to the ef...

journal_title：Biochemistry

authors： Zhang X,Donnelly A,Lee I,Berdis AJ

更新日期：2006-11-07 00:00:00

abstract：:Human type beta 2 transforming growth factor (hTGF-beta 2) was purified from tamoxifen-supplemented, serum-free medium conditioned by the human prostatic adenocarcinoma cell line PC-3. The purification of hTGF-beta 2 was monitored in a growth inhibition assay and was achieved by batch purification on methylsilyl-contr...

journal_title：Biochemistry

authors： Ikeda T,Lioubin MN,Marquardt H

更新日期：1987-05-05 00:00:00

abstract：:K(+) channels are revered for their universal action of suppressing electrical activity in nerve and muscle, as well as regulating salt and water transport in epithelial tissues involved in metabolism and digestion. These multisubunit membrane-embedded proteins carry out their physiological chore, selectively allowing...

journal_title：Biochemistry

authors： Ahern CA,Kobertz WR

更新日期：2009-01-27 00:00:00

abstract：:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...

journal_title：Biochemistry

authors： Holt AK,Senear DF

更新日期：2010-01-26 00:00:00