1H NMR observation of redox potential in liver.

abstract：:Attempts to modify the guanine specificity of ribonuclease T1 (RNase T1) by rationally designed amino acid substitutions failed so far. Therefore, we applied a semirational approach by randomizing the guanine binding site. A combinatorial library of approximately 1.6 million RNase T1 variants containing permutations o...

journal_title：Biochemistry

authors： Hubner B,Haensler M,Hahn U

更新日期：1999-01-26 00:00:00

abstract：:The steroidogenic acute regulatory protein (StAR) facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN-64 are deleted, the resul...

journal_title：Biochemistry

authors： Bose HS,Whittal RM,Huang MC,Baldwin MA,Miller WL

更新日期：2000-09-26 00:00:00

abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

journal_title：Biochemistry

authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

更新日期：1995-12-05 00:00:00

abstract：:The enzyme PvdQ (E.C. 3.5.1.97) from Pseudomonas aeruginosa is an N-terminal nucleophile hydrolase that catalyzes the removal of an N-myristyl substituent from a biosynthetic precursor of the iron-chelating siderophore pyoverdine. Inhibitors of pyoverdine biosynthesis are potential antibiotics since iron is essential ...

journal_title：Biochemistry

authors： Clevenger KD,Wu R,Liu D,Fast W

更新日期：2014-10-28 00:00:00

abstract：:To understand the relationship between the chemical structure of polar molecules and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related polar fluorescent probes was examined. The depth of these probes in lipid bilayers was determined by parallax analysis of fluorescence quen...

journal_title：Biochemistry

authors： Asuncion-Punzalan E,Kachel K,London E

更新日期：1998-03-31 00:00:00

abstract：:Using proton magnetic resonance, we have investigated imino and amino proton exchange in the Z form of the four oligomers d(Cbr8GCGCbr8G), d(CGm5CGCG), d(CG)6, and d(CG)12. In the latter two oligomers, all five exchangeable protons have been assigned. We find that proton acceptors such as NH3 or the basic form of Tris...

journal_title：Biochemistry

authors： Kochoyan M,Leroy JL,Guéron M

更新日期：1990-05-22 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...

journal_title：Biochemistry

authors： Bonetti D,Troilo F,Toto A,Brunori M,Longhi S,Gianni S

更新日期：2017-07-25 00:00:00

abstract：:The bacterial reaction center (RC) converts light into chemical energy through the reduction of an internal quinone molecule Q(B) to Q(B)H(2). In the native RC, proton transfer is coupled to electron transfer and is not rate-controlling. Consequently, proton transfer is not directly observable, and its rate was unknow...

journal_title：Biochemistry

authors： Paddock ML,Adelroth P,Feher G,Okamura MY,Beatty JT

更新日期：2002-12-17 00:00:00

abstract：:A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...

journal_title：Biochemistry

authors： Kowalczyk A,Carmical JR,Zou Y,Van Houten B,Lloyd RS,Harris CM,Harris TM

更新日期：2002-03-05 00:00:00

abstract：:Clostridium perfringens epsilon toxin belongs to the aerolysin-like family of pore-forming toxins and is one of the most potent bacterial toxins known. The epsilon toxin causes fatal enterotoxemia in sheep, goats, and possibly humans. Evidence indicates that the toxin binds to protein receptors including hepatitis A v...

journal_title：Biochemistry

authors： Ivie SE,McClain MS

更新日期：2012-09-25 00:00:00

abstract：:Oligomerization of numerous G protein-coupled receptors has been documented, including the prototypic family B secretin receptor. The clinical significance of oligomerization of this receptor became clear with the recent observation that a misspliced form present in pancreatic cancer could associate with the wild-type...

journal_title：Biochemistry

authors： Lisenbee CS,Miller LJ

更新日期：2006-07-11 00:00:00

abstract：:A general, combinatorial library method for the rapid identification of high-affinity peptide ligands of protein modular domains is reported. The validity of this method has been demonstrated by determining the sequence specificity of four Src homology 2 (SH2) domains derived from protein tyrosine phosphatase SHP-1 an...

journal_title：Biochemistry

authors： Sweeney MC,Wavreille AS,Park J,Butchar JP,Tridandapani S,Pei D

更新日期：2005-11-15 00:00:00

abstract：:The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...

journal_title：Biochemistry

authors： Lin SH,Lee JC

更新日期：2002-10-01 00:00:00

abstract：:Phosphinothricyl-alanyl-alanine (Pt tripeptide (Ptt), bialaphos) is a metabolite produced by Streptomyces viridochromogenes and Streptomyces hygroscopicus. It contains the unique phosphinoamino acid phosphinothricin (Pt), which after cleavage from Ptt is active as an inhibitor of glutamine synthetase. We have isolated...

journal_title：Biochemistry

authors： Grammel N,Schwartz D,Wohlleben W,Keller U

更新日期：1998-02-10 00:00:00

abstract：:Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...

journal_title：Biochemistry

authors： Vashishtha AK,Konigsberg WH

更新日期：2016-05-10 00:00:00

abstract：:The innate immune response to infection or injury involves an antigen-antibody triggered classical pathway (CP) of complement activation, in which soluble and cell surface plasma proteins cooperatively effect elimination of foreign organisms and damaged host cells. However, protracted or dysfunctional complement activ...

journal_title：Biochemistry

authors： Halili MA,Ruiz-Gómez G,Le GT,Abbenante G,Fairlie DP

更新日期：2009-09-08 00:00:00

abstract：:Membrane-bound phosphodiesterase 6 (PDE6) plays an important role in visual signal transduction by regulating cGMP levels in rod photoreceptor cells. Our understanding of PDE6 catalysis and structure suffers from inadequate characterization of the α and β subunit catalytic core, interactions of the core with two intri...

journal_title：Biochemistry

authors： Baker BY,Palczewski K

更新日期：2011-11-08 00:00:00

abstract：:G.U pairs occur frequently and have many important biological functions. The stability of symmetric tandem G.U motifs depends both on the adjacent Watson-Crick base pairs, e.g., 5'G > 5'C, and the sequence of the G.U pairs, i.e., 5'-UG-3' > 5'-GU-3', where an underline represents a nucleotide in a G.U pair [Wu, M., Mc...

journal_title：Biochemistry

authors： Chen X,McDowell JA,Kierzek R,Krugh TR,Turner DH

更新日期：2000-08-01 00:00:00

abstract：:Pineapple stem bromelain was photooxidized in the presence of Methylene Blue used as a sensitizer. The essential sulfhydryl group of the enzyme protein rapidly became inaccessible to react with 5,5'-dithiobis(nitrobenzoic acid), but the reactivity was readily regained to the original level upon treatment with dithioth...

journal_title：Biochemistry

authors： Murachi T,Tsudzuki T,Okumura K

更新日期：1975-01-28 00:00:00

abstract：:The ligand binding relationship between the acetylcholine transporter (AcChT) and the vesamicol receptor (VR) and the kinetics of active transport were studied in synaptic vesicles purified from the Torpedo electric organ using analogues of AcCh and vesamicol. Methoxyvesamicol, which should exhibit better equilibratio...

journal_title：Biochemistry

authors： Bahr BA,Clarkson ED,Rogers GA,Noremberg K,Parsons SM

更新日期：1992-06-30 00:00:00

abstract：:The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...

journal_title：Biochemistry

authors： Pilz I,Kratky O,Licht A,Sela M

更新日期：1975-03-25 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca2+-ATPase, has a pentacyclic indole tetramic acid scaffold that arises from one molecule of tryptophan, acetyl-CoA, malonyl-CoA, and dimethylallyl pyrophosphate by consecutive action of three enzymes, CpaS, CpaD, and CpaO. CpaS is a hybri...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-09-15 00:00:00

abstract：:Glutamate mutase is one of a group of adenosylcobalamin-dependent enzymes that catalyze unusual isomerizations that proceed through organic radical intermediates generated by homolytic fission of the coenzyme's unique cobalt-carbon bond. These enzymes are part of a larger family of enzymes that catalyze radical chemis...

journal_title：Biochemistry

authors： Cheng MC,Marsh EN

更新日期：2007-01-23 00:00:00

abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

journal_title：Biochemistry

authors： Richardson CJ,First EA

更新日期：2016-03-15 00:00:00

abstract：:DBA/2 mouse spleen cells were stimulated in vitro by (a) alloantigen (mitomycin-treated CBA/J splenocytes), (b) the T cell mitogen concanavalin A (Con A), and (c) the B cell mitogen lipopolysaccharide (LPS). The cultures were pulsed for 10 h with (14)C-labeled galactose and glucosamine. Radiolabeled glycosphingolipids...

journal_title：Biochemistry

authors： Gruner KR,van Eijk RV,Mühlradt PF

更新日期：1981-08-04 00:00:00

abstract：:Cytochrome c oxidase contains four redox-active metal centers: two heme irons, cytochromes a and a3, and two copper ions, CuA and CuB. Due to the paucity of spectroscopic signatures for both copper sites in cytochrome c oxidase, the ligands and structures for these sites have remained ambiguous. The specific depletion...

journal_title：Biochemistry

authors： Li PM,Gelles J,Chan SI,Sullivan RJ,Scott RA

更新日期：1987-04-21 00:00:00

abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...

journal_title：Biochemistry

authors： Chen T,Haigentz M Jr,Reisler E

更新日期：1992-03-24 00:00:00

abstract：:The major excreted protein (MEP) of transformed mouse fibroblasts has been purified, and monospecific antisera against it have been prepared. Synthesis and secretion of this protein have previously been shown to be stimulated by transformation or treatment with tumor-promoting phorbol esters, but its function is still...

journal_title：Biochemistry

authors： Gottesman MM,Cabral F

更新日期：1981-03-17 00:00:00

abstract：:Most nuclear receptors recognize the same consensus hexameric sequence, AGGTCA. An important question has been how the various members of this transcription factor family distinguish identity features in these closely related DNA sites. We determined structures from several crystal forms of the RevErb-DNA complex and ...

journal_title：Biochemistry

authors： Sierk ML,Zhao Q,Rastinejad F

更新日期：2001-10-30 00:00:00

abstract：:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...

journal_title：Biochemistry

authors： Thornton K,Gorenstein DG

更新日期：1994-03-29 00:00:00