Intrastrand DNA cross-links as tools for studying DNA replication and repair: two-, three-, and four-carbon tethers between the N(2) positions of adjacent guanines.

abstract：:Folding models suggest that the highly conserved histidine 217 of the cytochrome b subunit from the cytochrome bc1 complex is close to the quinone reductase (Qi) site. This histidine (bH217) in the cytochrome b polypeptide of the photosynthetic bacterium Rhodobacter capsulatus has been replaced with three other residu...

journal_title：Biochemistry

authors： Gray KA,Dutton PL,Daldal F

更新日期：1994-01-25 00:00:00

abstract：:The natural product hypericin is a photosensitive polycyclic aromatic dione compound, which has been widely investigated because of its virucidal and antitumor properties. Although it has been suggested that singlet oxygen or a radical species might be responsible for its biological action, its mechanism of action rem...

journal_title：Biochemistry

authors： Weiner L,Roth E,Mazur Y,Silman I

更新日期：1999-08-31 00:00:00

abstract：:The spin-1/2-carrying NO molecule interacts with both the S1 and S2 states of the water oxidizing complex. The intermediates of the interaction can be resolved and trapped by NO treatment at subzero temperatures. At -30 degrees C and in the presence of approx. 500-700 microM NO, S1 loses the ability to yield by illumi...

journal_title：Biochemistry

authors： Goussias C,Ioannidis N,Petrouleas V

更新日期：1997-07-29 00:00:00

abstract：:PhnZ utilizes a mixed valence diiron(II/III) cofactor and O2 to oxidatively cleave the carbon-phosphorus bond of (R)-2-amino-1-hydroxyethylphosphonic acid to form glycine and orthophosphate. The active site residues Y24 and E27 are proposed to mediate induced-fit recognition of the substrate and access of O2 to one of...

journal_title：Biochemistry

authors： Gama SR,Lo BSY,Séguin J,Pallitsch K,Hammerschmidt F,Zechel DL

更新日期：2019-12-31 00:00:00

abstract：:The amino acid sequence of the sex steroid binding protein (SBP) from human plasma has been determined. The SBP subunit consists of a 373-residue polypeptide chain containing two disulfide bonds and three oligosaccharide chains. The sequence was solved primarily by analysis of peptides derived by cleavage at either ly...

journal_title：Biochemistry

authors： Walsh KA,Titani K,Takio K,Kumar S,Hayes R,Petra PH

更新日期：1986-11-18 00:00:00

abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：1999-03-09 00:00:00

abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...

journal_title：Biochemistry

authors： Caffrey M

更新日期：1987-10-06 00:00:00

abstract：:The interactions of the ligand binding domain (LBD) of androgen receptor (AR) and the AR T877A mutant, found in prostate cancer, with peptides from coactivator and corepressor proteins or random phage display peptides were investigated using in vitro time-resolved fluorescence resonance energy transfer (TR-FRET). Inte...

journal_title：Biochemistry

authors： Ozers MS,Marks BD,Gowda K,Kupcho KR,Ervin KM,De Rosier T,Qadir N,Eliason HC,Riddle SM,Shekhani MS

更新日期：2007-01-23 00:00:00

abstract：:Two photoreactive apamin derivatives were prepared with an aryl azide [[(azidonitrophenyl)amino]acetate (ANPAA)] group coupled at different positions on the neurotoxin molecule. These ligands were used to identify membrane components in the environment of the neuronal binding site that is associated with a Ca2+-activa...

journal_title：Biochemistry

authors： Seagar MJ,Labbé-Jullié C,Granier C,Goll A,Glossmann H,Van Rietschoten J,Couraud F

更新日期：1986-07-15 00:00:00

abstract：:Tandem calponin-homology (CH) domains are the most common actin-binding domains in proteins. However, structural principles underlying their function are poorly understood. These tandem domains exist in multiple conformations with varying degrees of inter-CH-domain interactions. Dystrophin and utrophin tandem CH domai...

journal_title：Biochemistry

authors： Bandi S,Singh SM,Mallela KM

更新日期：2015-09-08 00:00:00

abstract：:A chromatin acceptor protein for the avian oviduct progesterone receptor (PR), termed receptor binding factor 1 (RBF-1), has recently been shown to (1) be a component of the nuclear binding sites (acceptor sites) for PR and (2) generate high-affinity binding sites (termed the RBF-1 class of sites) on avian genomic DNA...

journal_title：Biochemistry

authors： Schuchard M,Subramaniam M,Ruesink T,Spelsberg TC

更新日期：1991-10-01 00:00:00

abstract：:Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...

journal_title：Biochemistry

authors： Mursinna RS,Lincecum TL Jr,Martinis SA

更新日期：2001-05-08 00:00:00

abstract：:The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...

journal_title：Biochemistry

authors： Noble RW,DeYoung A,Rousseau DL

更新日期：1989-06-13 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

journal_title：Biochemistry

authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

更新日期：1998-05-12 00:00:00

abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...

journal_title：Biochemistry

authors： Nordell P,Jansson ET,Lincoln P

更新日期：2009-02-24 00:00:00

abstract：:How various anti-amyloidogenic compounds inhibit the formation of Alzheimer's beta-amyloid fibrils (fAbeta) from amyloid beta-peptide (Abeta) and destabilize fAbeta remains poorly understood. Using spectrophotometry, spectrofluorometry, atomic force microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis...

journal_title：Biochemistry

authors： Hirohata M,Hasegawa K,Tsutsumi-Yasuhara S,Ohhashi Y,Ookoshi T,Ono K,Yamada M,Naiki H

更新日期：2007-02-20 00:00:00

abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

journal_title：Biochemistry

authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

更新日期：2002-06-04 00:00:00

abstract：:Although the Escherichia coli fatty acid synthesis (FAS) pathway is the best studied type II fatty acid synthesis system, a major experimental limitation has been the inability to feed intermediates into the pathway in vivo because exogenously supplied free fatty acids are not efficiently converted to the acyl-acyl ca...

journal_title：Biochemistry

authors： Jiang Y,Morgan-Kiss RM,Campbell JW,Chan CH,Cronan JE

更新日期：2010-02-02 00:00:00

abstract：:In an effort to extend automated Edman degradation to nanomole quantities of protein, the method of sequenator analysis described by Edman and Begg (Edman, P., and Begg, G. (1967), Eur. J. Biochem. 1, 80) was modified to permit long degradations in the absence of carrier proteins. By using an aqueous 0.1 M Quadrol pro...

journal_title：Biochemistry

authors： Brauer AW,Margolies MN,Haber E

更新日期：1975-07-01 00:00:00

abstract：:Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...

journal_title：Biochemistry

authors： Witt JJ,Roskoski R Jr

更新日期：1980-01-08 00:00:00

abstract：:Candida albicans drug resistance protein 1 (Cdr1p), an ATP-dependent drug efflux pump, contributes to multidrug resistance in Candida-infected immunocompromised patients. Previous cell-based assays suggested that Cdr1p also acts as a phospholipid translocator. To investigate this, we reconstituted purified Cdr1p into ...

journal_title：Biochemistry

authors： Shukla S,Rai V,Saini P,Banerjee D,Menon AK,Prasad R

更新日期：2007-10-30 00:00:00

abstract：:DNA gyrase is the target of a number of antibacterial agents, including the coumarins and the cyclothialidines. To extend our understanding of the mechanism of action of these compounds, we have examined the previously published crystal structures of the complexes between the 24 kDa fragment of GyrB and coumarin and c...

journal_title：Biochemistry

authors： Kampranis SC,Gormley NA,Tranter R,Orphanides G,Maxwell A

更新日期：1999-02-16 00:00:00

abstract：:Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...

journal_title：Biochemistry

authors： Ruan KH,So SP,Wu J,Li D,Huang A,Kung J

更新日期：2001-01-09 00:00:00

abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...

journal_title：Biochemistry

authors： Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

更新日期：1989-03-21 00:00:00

abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...

journal_title：Biochemistry

authors： Laberge M,Osvath S,Fidy J

更新日期：2001-08-07 00:00:00

abstract：:The fatty acid synthase (FAS) of animal tissue is a dimer of two identical subunits, each with a Mr of 260,000. The subunit is a single multifunctional protein having seven catalytic activities and a site for binding of the prosthetic group 4'-phosphopantetheine. The mRNA coding for the subunit has an estimated size o...

journal_title：Biochemistry

authors： Kasturi R,Chirala S,Pazirandeh M,Wakil SJ

更新日期：1988-10-04 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...

journal_title：Biochemistry

authors： Lu T,Tan H,Lee D,Chen G,Jia Z

更新日期：2009-08-25 00:00:00

abstract：:An oligodeoxynucleotide containing four thymines and covalently attached to an acridine derivative through its 3'-phosphate [(Tp)4(CH2)5Acr] was synthesized. Its conformation in solution was investigated by proton magnetic resonance. Both intramolecular interactions between the acridine dye and thymines and intermolec...

journal_title：Biochemistry

authors： Lancelot G,Asseline U,Thuong NT,Hélène C

更新日期：1985-05-07 00:00:00

abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...

journal_title：Biochemistry

authors： Hutchens TW,Porath J

更新日期：1987-11-03 00:00:00