Abstract:
:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further saturation is on the other hand kinetically hampered in comparison to the relaxed DNA. We also observe that threading kinetics correlates with the inhibition of luciferase expression from the plasmid construct. The results show how the target torsional strain can function as a control of DNA threading kinetics and gene expression efficiency.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nordell P,Jansson ET,Lincoln Pdoi
10.1021/bi802336psubject
Has Abstractpub_date
2009-02-24 00:00:00pages
1442-4issue
7eissn
0006-2960issn
1520-4995pii
10.1021/bi802336pjournal_volume
48pub_type
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