Supercoil-accelerated DNA threading intercalation.

Abstract:

:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further saturation is on the other hand kinetically hampered in comparison to the relaxed DNA. We also observe that threading kinetics correlates with the inhibition of luciferase expression from the plasmid construct. The results show how the target torsional strain can function as a control of DNA threading kinetics and gene expression efficiency.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Nordell P,Jansson ET,Lincoln P

doi

10.1021/bi802336p

subject

Has Abstract

pub_date

2009-02-24 00:00:00

pages

1442-4

issue

7

eissn

0006-2960

issn

1520-4995

pii

10.1021/bi802336p

journal_volume

48

pub_type

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