Proton and phosphorus nuclear magnetic resonance studies of an oligothymidylate covalently linked to an acridine derivative and of its binding to complementary sequences.

abstract：:Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...

journal_title：Biochemistry

authors： Clark DD,Ensign SA

更新日期：2002-02-26 00:00:00

abstract：:The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subun...

journal_title：Biochemistry

authors： Mazzoni MR,Hamm HE

更新日期：1989-12-12 00:00:00

abstract：:The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...

journal_title：Biochemistry

authors： Jones MK,Catte A,Li L,Segrest JP

更新日期：2009-12-01 00:00:00

abstract：:The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large ...

journal_title：Biochemistry

authors： Abonyo BO,Gou D,Wang P,Narasaraju T,Wang Z,Liu L

更新日期：2004-03-30 00:00:00

abstract：:Three electrochromic difference spectra induced by the deposition of (1) a negative charge on the primary quinone acceptor, Q(A), (2) a positive charge on (or near) Tyr161 of the D1 subunit (Y(Z)), and (3) a positive charge on the manganese cluster were determined at room temperature in photosystem II (PSII) core part...

journal_title：Biochemistry

authors： Mulkidjanian AY,Cherepanov DA,Haumann M,Junge W

更新日期：1996-03-05 00:00:00

abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

journal_title：Biochemistry

authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

更新日期：2008-04-22 00:00:00

abstract：:Factor X (FX) is one of the major players in the blood coagulation cascade. Upon activation to FXa, it converts prothrombin to thrombin, which in turn converts fibrinogen into fibrin (blood clots). FXa deficiency causes hemostasis defects, such as intracranial bleeding, hemathrosis, and gastrointestinal blood loss. He...

journal_title：Biochemistry

authors： Abdel-Azeim S,Oliva R,Chermak E,De Cristofaro R,Cavallo L

更新日期：2014-11-11 00:00:00

abstract：:This work describes the biochemical characterization of the catalytic domain of Ira2p, a Saccharomyces cerevisiae GTPase-activating protein (GAP) regulating the RAS gene products. A fragment of 383 residues (amino acids 1644-2026) was produced in Escherichia coli as glutathione S-transferase fusion protein (GST-Ira2p-...

journal_title：Biochemistry

authors： Parrini MC,Jacquet E,Bernardi A,Jacquet M,Parmeggiani A

更新日期：1995-10-24 00:00:00

abstract：:Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. Th...

journal_title：Biochemistry

authors： Song J,Hagen GM,Roess DA,Pecht I,Barisas BG

更新日期：2002-01-22 00:00:00

abstract：:We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis...

journal_title：Biochemistry

authors： Nathanson NM,Hall ZW

更新日期：1979-07-24 00:00:00

abstract：:A chromatin acceptor protein for the avian oviduct progesterone receptor (PR), termed receptor binding factor 1 (RBF-1), has recently been shown to (1) be a component of the nuclear binding sites (acceptor sites) for PR and (2) generate high-affinity binding sites (termed the RBF-1 class of sites) on avian genomic DNA...

journal_title：Biochemistry

authors： Schuchard M,Subramaniam M,Ruesink T,Spelsberg TC

更新日期：1991-10-01 00:00:00

abstract：:The mitochondrial folate transporter (MFT) was previously identified in human and hamster cells. Sequence homology of this protein with the inner mitochondrial membrane transporters suggested a domain structure in which the N- and C-termini of the protein are located on the mitochondrial intermembrane-facing surface, ...

journal_title：Biochemistry

authors： Perchiniak E,Lawrence SA,Kasten S,Woodard BA,Taylor SM,Moran RG

更新日期：2007-02-13 00:00:00

abstract：:Nitric oxide synthases (NOSs) are proposed to generate NO and citrulline from L-arginine in two steps: initial N-hydroxylation to generate Nomega-hydroxyarginine (NOHA) followed by a three-electron oxidation of the hydroxylated nitrogen to form products. Both steps consume NADPH and may involve heme iron-based activat...

journal_title：Biochemistry

authors： Abu-Soud HM,Presta A,Mayer B,Stuehr DJ

更新日期：1997-09-09 00:00:00

abstract：:Nucleotide-induced conformational changes of the catalytic beta subunits play a crucial role in the rotary mechanism of F(1)-ATPase. To gain insights into the energetic bases that govern the recognition of nucleotides by the isolated beta subunit from thermophilic Bacillus PS3 (Tbeta), the binding of this monomer to M...

journal_title：Biochemistry

authors： Pulido NO,Salcedo G,Pérez-Hernández G,José-Núñez C,Velázquez-Campoy A,García-Hernández E

更新日期：2010-06-29 00:00:00

abstract：:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...

journal_title：Biochemistry

authors： Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

更新日期：2009-06-16 00:00:00

abstract：:The molecular mechanism for the displacement of HMGA1 proteins from DNA is integral to disrupting their cellular function, which is linked to many metastatic cancers. Chemical shift and NOESY NMR experiments provide structural evidence for the displacement of an AT hook peptide (DNA binding motif of HMGA1 proteins) by...

journal_title：Biochemistry

authors： Smith AE,Buchmueller KL

更新日期：2011-09-27 00:00:00

abstract：:Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through...

journal_title：Biochemistry

authors： Boggs JM,Rangaraj G,Gao W,Heng YM

更新日期：2006-01-17 00:00:00

abstract：:The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...

journal_title：Biochemistry

authors： Ullner M,Selander M,Persson E,Stenflo J,Drakenberg T,Teleman O

更新日期：1992-07-07 00:00:00

abstract：:Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...

journal_title：Biochemistry

authors： Song H,Bao S,Ramanadham S,Turk J

更新日期：2006-05-23 00:00:00

abstract：:The glucose derivative, 2,2,6,6-tetramethylpiperidine-1-oxylglucose (TEMPO-glucose) was synthesized and examined for its ability to substitute for glucose as a substrate for the intestinal brush border membrane Na+/glucose cotransporter. TEMPO-glucose inhibited Na(+)-dependent phlorizin binding with an apparent KI of ...

journal_title：Biochemistry

authors： Peerce BE

更新日期：1991-04-30 00:00:00

abstract：:Phosphorylation of the sarcoplasmic reticulum calcium ATPase, E, is first order with kb = 70 +/- 7 s-1 after free enzyme was mixed with saturating ATP and 50 microM Ca2+; this is one-third the rate constant of 220 s-1 for phosphorylation of enzyme preincubated with calcium, cE.Ca2, after being mixed with ATP under the...

journal_title：Biochemistry

authors： Stahl N,Jencks WP

更新日期：1987-12-01 00:00:00

abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...

journal_title：Biochemistry

authors： Longstaff C,Gaffney PJ

更新日期：1991-01-29 00:00:00

abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...

journal_title：Biochemistry

authors： Orban T,Huang CC,Homan KT,Jastrzebska B,Tesmer JJ,Palczewski K

更新日期：2012-04-24 00:00:00

abstract：:To investigate the effect of a reduced level of selenocysteine (Sec) tRNA[Ser]Sec in selenoprotein biosynthesis, two mouse embryonic stem (ES) cell lines heterozygous for the corresponding gene were generated by homologous recombination of the host genome with targeting vectors encoding a deleted or a disrupted tRNA[S...

journal_title：Biochemistry

authors： Chittum HS,Baek HJ,Diamond AM,Fernandez-Salguero P,Gonzalez F,Ohama T,Hatfield DL,Kuehn M,Lee BJ

更新日期：1997-07-15 00:00:00

abstract：:Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...

journal_title：Biochemistry

authors： Karran P,Higgins NP,Strauss B

更新日期：1977-10-04 00:00:00

abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

journal_title：Biochemistry

authors： Flock U,Watmough NJ,Adelroth P

更新日期：2005-08-09 00:00:00

abstract：:Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purificati...

journal_title：Biochemistry

authors： Brodner OG,Wieland T

更新日期：1976-08-10 00:00:00

abstract：:Cells that depend on oxygen for survival constantly produce reactive oxygen species that attack DNA to produce a variety of lesions, including single-strand breaks with 3'-blocking groups such as 3'-phosphate and 3'-phosphoglycolate. These 3'-blocking ends prevent the activity of DNA polymerase and are generally remov...

journal_title：Biochemistry

authors： Jilani A,Ramotar D

更新日期：2002-06-18 00:00:00

abstract：:Asymmetrically modified hemoglobins, [alpha(des-Arg)beta]A[alpha beta]cXL, [alpha(des-Arg-Tyr)beta]A[alpha beta]cXL, [alpha(des-Arg)beta(NES)]A[alpha beta]cXL, and [alpha(des-Arg)beta]A[alpha beta(NES)]cXL, have been prepared from chemically modified human normal adult hemoglobin (Hb A) and mutant hemoglobin C (beta 6...

journal_title：Biochemistry

authors： Miura S,Ho C

更新日期：1984-05-22 00:00:00

abstract：:We have characterized the importance of size, sulfation, and anticoagulant activity of heparin in release of basic fibroblast growth factor (bFGF) from the subendothelial extracellular matrix (ECM) and the luminal surface of the vascular endothelium. For this purpose, 125I-bFGF was first incubated with ECM and conflue...

journal_title：Biochemistry

authors： Ishai-Michaeli R,Svahn CM,Weber M,Chajek-Shaul T,Korner G,Ekre HP,Vlodavsky I

更新日期：1992-02-25 00:00:00