Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion.

abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...

journal_title：Biochemistry

authors： Li YC,Zhang X,Melton R,Ganu V,Gonnella NC

更新日期：1998-10-06 00:00:00

abstract：:Platelet receptor recognition domains are located on the gamma and alpha chains of human fibrinogen. The former encompasses residues 400-411 [Kloczewiak, M., Timmons, S., Lukas, T. J., & Hawiger, J. (1984) Biochemistry 23, 1767], and the latter is present in two loci on the alpha chain (alpha 95-97 and alpha 572-574) ...

journal_title：Biochemistry

authors： Timmons S,Bednarek MA,Kloczewiak M,Hawiger J

更新日期：1989-04-04 00:00:00

abstract：:The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate gro...

journal_title：Biochemistry

authors： Huddleston JP,Raushel FM

更新日期：2019-09-17 00:00:00

abstract：:Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational ensemble of the tandemly repeating tetrapeptide unit of the circumsporozoite coat protein of the malaria parasite Plasmodium falciparum. Peptides based on the Asn-Ala-Asn-Pro and Asn-Pro-Asn-Ala...

journal_title：Biochemistry

authors： Dyson HJ,Satterthwait AC,Lerner RA,Wright PE

更新日期：1990-08-28 00:00:00

abstract：:Kinetic and structural data are presented on the interaction with Torpedo californica acetylcholinesterase (TcAChE) of (+)-huperzine A, a synthetic enantiomer of the anti-Alzheimer drug, (-)-huperzine A, and of its natural homologue (-)-huperzine B. (+)-Huperzine A and (-)-huperzine B bind to the enzyme with dissociat...

journal_title：Biochemistry

authors： Dvir H,Jiang HL,Wong DM,Harel M,Chetrit M,He XC,Jin GY,Yu GL,Tang XC,Silman I,Bai DL,Sussman JL

更新日期：2002-09-03 00:00:00

abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

journal_title：Biochemistry

authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

更新日期：1994-09-20 00:00:00

abstract：:A new strategy for the assignment of nonexchangeable proton resonances in oligonucleotide duplexes is presented and used to interpret the spectra of the oligonucleotide helix d(CpGpCpGpCpG) X d(CpGpCpGpCpG) in low salt (B form). This procedure is based on the use of sequential homodecoupling (1D) or COSY (2D) for the ...

journal_title：Biochemistry

authors： Frechet D,Cheng DM,Kan LS,Ts'o PO

更新日期：1983-10-25 00:00:00

abstract：:A detailed kinetic analysis of the catalytic trimer of aspartate transcarbamoylase containing the active site substitution H134A was performed to investigate the role of His 134 in the catalytic mechanism. Replacement of histidine by alanine resulted in decreases in the affinities for the two substrates, carbamoyl pho...

journal_title：Biochemistry

authors： Waldrop GL,Turnbull JL,Parmentier LE,O'Leary MH,Cleland WW,Schachman HK

更新日期：1992-07-21 00:00:00

abstract：:The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...

journal_title：Biochemistry

authors： Luck LA,Falke JJ

更新日期：1991-04-30 00:00:00

abstract：:Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...

journal_title：Biochemistry

authors： Venugopal H,Edwards PJ,Schwalbe M,Claridge JK,Libich DS,Stepper J,Loo T,Patchett ML,Norris GE,Pascal SM

更新日期：2011-04-12 00:00:00

abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

journal_title：Biochemistry

authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

更新日期：2020-02-04 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a type II DHFR produced by bacteria as a resistance mechanism to the increased clinical use of the antibacterial drug trimethoprim. Type II DHFRs are not homologous in either sequence or structure with chromosomal DHFRs. The type II enzymes contain four identical subunits which fo...

journal_title：Biochemistry

authors： Li D,Levy LA,Gabel SA,Lebetkin MS,DeRose EF,Wall MJ,Howell EE,London RE

更新日期：2001-04-10 00:00:00

abstract：:We have measured the concentration of mRNAs coding for immunoglobulins, k and lambda type light chains and gamma 1 type heavy chain, in mouse spleen cells activated by bacterial lipopolysaccharide or sheep red blood cells. These mRNAs were quantitated by hybridization to radioactive DNA complementary to highly purifie...

journal_title：Biochemistry

authors： Honjo T,Shimizu A,Tsuda M,Natori S,Katatoka T,Dohmoto C,Mano Y

更新日期：1977-12-27 00:00:00

abstract：:The formation of tyrosine from phenylalanine catalyzed by rat liver phenylalanine hydroxylase is coupled to the generation of a 4a-hydroxy adduct from the requisite tetrahydropterin cofactor. As indicated by its circular dichroism (CD) spectrum, the optical activity of the adduct generated from racemic 6-methyltetrahy...

journal_title：Biochemistry

authors： Dix TA,Bollag GE,Domanico PL,Benkovic SJ

更新日期：1985-06-04 00:00:00

abstract：:In our previous work [Yang, Z. W., & Babitch, J. A. (1988) Biochemistry (preceding paper in this issue)] divalent cations were found to be more effective promoters of astroglial filament formation than were monovalent cations. To determine if one or more divalent cation binding sites were the basis for this difference...

journal_title：Biochemistry

authors： Yang ZW,Kong CF,Babitch JA

更新日期：1988-09-06 00:00:00

abstract：:Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) ...

journal_title：Biochemistry

authors： Payne G,Sancar A

更新日期：1990-08-21 00:00:00

abstract：:Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...

journal_title：Biochemistry

authors： Grzyska PK,Ryle MJ,Monterosso GR,Liu J,Ballou DP,Hausinger RP

更新日期：2005-03-15 00:00:00

abstract：:Recent studies on cytochrome oxidase have indicated that the putative "peroxy" intermediate in the catalytic cycle (P(R)) is a mixture of intermediates, including P and F [Sucheta, A., et al. (1998) Biochemistry 37, 17905-17914], and the bench-made P and F forms appear to have the same redox state (Fe(a3)(4+)=O(2-)), ...

journal_title：Biochemistry

authors： Van Eps N,Szundi I,Einarsdóttir O

更新日期：2003-05-06 00:00:00

abstract：:Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...

journal_title：Biochemistry

authors： Leu LS,Cook PF

更新日期：1994-03-08 00:00:00

abstract：:The causes of the salting-in of beta-lactoglobulin by glycine and NaCl, a solubility behavior contrary to expectations, were probed by a detailed study of the interactions between these solvent components and the protein. The preferential interactions of beta-lactoglobulin with solvent components in aqueous glycine an...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1987-08-11 00:00:00

abstract：:Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...

journal_title：Biochemistry

authors： Kakuyama H,Söderberg L,Horigome K,Winblad B,Dahlqvist C,Näslund J,Tjernberg LO

更新日期：2005-11-29 00:00:00

abstract：:Bovine milk lipoprotein lipase (LpL) catalyzes the hydrolysis of the water-soluble esters p-nitrophenyl acetate (PNPA) and p-nitrophenyl butyrate (PNPB). The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol h...

journal_title：Biochemistry

authors： Quinn DM,Shirai K,Jackson RL,Harmony JA

更新日期：1982-12-21 00:00:00

abstract：:A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...

journal_title：Biochemistry

authors： Griep MA,Adkins BJ,Hromas D,Johnson S,Miller J

更新日期：1997-01-21 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

journal_title：Biochemistry

authors： Laughrea M,Tam J

更新日期：1991-12-03 00:00:00

abstract：:DNA-protein cross-links (DPCs) in nucleosome core particles (NCPs), the fundamental building block of chromatin, arise during times of cellular oxidative stress. These lesions are expected to be detrimental to the cell due to interference with processes like chromatin remodeling, transcription, DNA replication, and ep...

journal_title：Biochemistry

authors： Bjorklund CC,Davis WB

更新日期：2007-09-25 00:00:00

abstract：:HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...

journal_title：Biochemistry

authors： Velten M,Villoutreix BO,Ladjimi MM

更新日期：2000-01-18 00:00:00

abstract：:Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...

journal_title：Biochemistry

authors： Wang L,Tharp S,Selzer T,Benkovic SJ,Kohen A

更新日期：2006-02-07 00:00:00

abstract：:To better elucidate RNA structure-function relationships and to improve the design of pharmaceutical agents that target specific RNA motifs, an understanding of RNA primary, secondary, and tertiary structure is necessary. The prediction of RNA secondary structure from sequence is an intermediate step in predicting RNA...

journal_title：Biochemistry

authors： Hausmann NZ,Znosko BM

更新日期：2012-07-03 00:00:00

abstract：:Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...

journal_title：Biochemistry

authors： Tranguch AJ,Kindelberger DW,Rohlman CE,Lee JY,Engelke DR

更新日期：1994-02-22 00:00:00