N-Terminal Region of Vibrio parahemolyticus Thermostable Direct Hemolysin Regulates the Membrane-Damaging Action of the Toxin.

abstract：:Two compounds have been synthesized that feature a photosensitive o-nitrobenzyl moiety attached directly to the carbamate nitrogen of carbamoylcholine. The well-characterized acetylcholine analogue, carbamoylcholine, was released from these derivatives in response to laser light pulses at wavelengths between 300 and 3...

journal_title：Biochemistry

authors： Walker JW,McCray JA,Hess GP

更新日期：1986-04-08 00:00:00

abstract：:Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...

journal_title：Biochemistry

authors： Wu F,Flach CR,Seaton BA,Mealy TR,Mendelsohn R

更新日期：1999-01-12 00:00:00

abstract：:The E2 protein of papillomavirus is the key regulator of viral transcription and replication. Dimerization, which takes place via its conserved C-terminal DNA-binding domain (DBD), is critical for these functions. The presence and conservation of two histidines (H290 and H320) at or near the dimer interface suggests t...

journal_title：Biochemistry

authors： Bose K,Yoder NC,Kumar K,Baleja JD

更新日期：2007-02-06 00:00:00

abstract：:Introduction of a disulfide bond by site-directed mutagenesis was found to enhance the stability of subtilisin BPN' (EC 3.4.21.14) under a variety of conditions. The location of the new disulfide bond was selected with the aid of a computer program, which scored various sites according to the amount of distortion that...

journal_title：Biochemistry

authors： Pantoliano MW,Ladner RC,Bryan PN,Rollence ML,Wood JF,Poulos TL

更新日期：1987-04-21 00:00:00

abstract：:In the DNA recognition mode of C(2)H(2)-type zinc fingers, the finger-finger connection region, consisting of the histidine spacing (HX(3-5)H) and linker, would be important for determining the orientation of the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNA binding,...

journal_title：Biochemistry

authors： Shiraishi Y,Imanishi M,Sugiura Y

更新日期：2004-05-25 00:00:00

abstract：:Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...

journal_title：Biochemistry

authors： Hui HL,Kavanaugh JS,Doyle ML,Wierzba A,Rogers PH,Arnone A,Holt JM,Ackers GK,Noble RW

更新日期：1999-01-19 00:00:00

abstract：:c-Myc is a member of a family of sequence specific-DNA binding proteins that are thought to regulate the transcription of genes involved in normal cell growth, differentiation, and apoptosis. In order to understand how human c-myc functions as a transcription factor, we have studied the mechanism of action and structu...

journal_title：Biochemistry

authors： McEwan IJ,Dahlman-Wright K,Ford J,Wright AP

更新日期：1996-07-23 00:00:00

abstract：:Previous crystallographic studies in this laboratory demonstrated that immunoglobulin light chains with the same amino acid sequence can have at least two and probably three or more conformations, depending on whether the second member of an interacting pair is a light or heavy chain. If a heavy chain is not available...

journal_title：Biochemistry

authors： Firca JR,Ely KR,Kremser P,Westholm FA,Dorrington KJ,Edmundson AB

更新日期：1978-01-10 00:00:00

abstract：:Three modified hammerhead ribozyme/substrate complexes have been prepared in which individual uridine O2-carbonyls have been eliminated. The modified complexes were chemically synthesized with the substitution of a single 2-pyridone (2P) base analogue for residues U4, U7, and U16.1. Steady-state kinetic analyses indic...

journal_title：Biochemistry

authors： Bevers S,Ha SB,McLaughlin LW

更新日期：1999-06-15 00:00:00

abstract：:A series of sulfonate esters that are attached to a noncationic minor-groove-binding N-methylpyrrole dipeptide (Lex) related to netrospin have been synthesized. The compounds prepared differ in two respects: (1) the length [(CH2)2 vs (CH2)8] of the tether between the DNA affinity binding portion of the molecule and th...

journal_title：Biochemistry

authors： Zhang Y,Chen FX,Mehta P,Gold B

更新日期：1993-08-10 00:00:00

abstract：:Bacteriophage P22 is a double-stranded DNA containing phage. Its morphogenetic pathway requires the formation of a precursor procapsid that subsequently matures to the capsid. The stability of bacteriophage P22 coat protein in both monomeric and polymeric forms under hydrostatic pressure has been examined previously [...

journal_title：Biochemistry

authors： Foguel D,Teschke CM,Prevelige PE Jr,Silva JL

更新日期：1995-01-31 00:00:00

abstract：:Site-directed mutagenesis was used to construct mutant Trp repressors with each of the 38 possible single amino acid changes of the first 2 amino acid residues (Ile79 and Ala80) in the second "recognition" alpha-helix of the helix-turn-helix DNA-binding motif. Eight of these mutant repressors with Ile79 and Ala80 chan...

journal_title：Biochemistry

authors： Pfau J,Arvidson DN,Youderian P,Pearson LL,Sigman DS

更新日期：1994-09-20 00:00:00

abstract：:We describe a novel, potent peptide substrate mimetic inhibitor of protein kinase B (PKB/Akt). The compound selectively kills prostate cancer cells, in which PKB is highly activated, but not normal cells, or cancer cells in which PKB is not activated. The inhibitor induces apoptosis and inhibits the phosphorylation of...

journal_title：Biochemistry

authors： Litman P,Ohne O,Ben-Yaakov S,Shemesh-Darvish L,Yechezkel T,Salitra Y,Rubnov S,Cohen I,Senderowitz H,Kidron D,Livnah O,Levitzki A,Livnah N

更新日期：2007-04-24 00:00:00

abstract：:The intracellular level of beta-hydroxydecanoyl thio ester dehydrase, the product of the fabA gene of Escherichia coli, was increased by isolation of a putative promotor mutant (termed fabAup) or by molecular cloning of the wild-type fabA gene into plasmid pBR322. The fabAup and plasmid-carrying strains overproduced d...

journal_title：Biochemistry

authors： Clark DP,DeMendoza D,Polacco ML,Cronan JE Jr

更新日期：1983-12-06 00:00:00

abstract：:Guanidinium chloride- (GdmCl-) induced unfolding of beta-lactamase has been investigated by a combination of size-exclusion chromatography (SEC-FPLC) and usual optical methods. It has been shown that at low temperatures this protein unfolds through two equilibrium intermediates. The first of these intermediates is the...

journal_title：Biochemistry

authors： Uversky VN,Ptitsyn OB

更新日期：1994-03-15 00:00:00

abstract：:The toxin RelE is a ribosome-dependent endoribonuclease implicated in diverse cellular processes, including persistence. During amino acid starvation, RelE inhibits translation by cleaving ribosomal A-site mRNA. Although RelE is structurally similar to other microbial endoribonucleases, the active-site amino acid comp...

journal_title：Biochemistry

authors： Griffin MA,Davis JH,Strobel SA

更新日期：2013-12-03 00:00:00

abstract：:Glycosylation is a common modification that can endow proteins with altered physical and biological properties. Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88. We have produced the three individual glycofor...

journal_title：Biochemistry

authors： Kilgore HR,Latham AP,Ressler VT,Zhang B,Raines RT

更新日期：2020-09-01 00:00:00

abstract：:The structural domains of human apolipoprotein(a) [apo(a)] and its interaction with apolipoprotein B-100 (apo B-100) in the lipoprotein(a) [Lp(a)] particle were investigated by limited proteolysis with thermolysin and cathepsin D. We characterized the proteolytic products by sodium dodecyl sulfate-polyacrylamide gradi...

journal_title：Biochemistry

authors： Huby T,Doucet C,Dieplinger H,Chapman J,Thillet J

更新日期：1994-03-22 00:00:00

abstract：:Much interest is currently focused on understanding the detailed contribution that particular amino acid residues make in protein structure and function. Although the use of site-directed mutagenesis has greatly contributed to this goal, the approach is limited to the standard repertoire of twenty amino acids. Fluorin...

journal_title：Biochemistry

authors： Duewel H,Daub E,Robinson V,Honek JF

更新日期：1997-03-18 00:00:00

abstract：:Syk, a nonreceptor protein-tyrosine kinase, is activated by both oxidative and osmotic stress and plays different roles in the transduction of stress signals. In this study, the regulation of oxidative and osmotic stress induced Syk activation was investigated utilizing Syk-negative DT40 cells, expressing various Syk ...

journal_title：Biochemistry

authors： Qin S,Kurosaki T,Yamamura H

更新日期：1998-04-21 00:00:00

abstract：:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...

journal_title：Biochemistry

authors： Howe L,Itoh T,Katagiri C,Ausio J

更新日期：1998-02-03 00:00:00

abstract：:Aminoacyl-tRNA synthetases preserve the fidelity of decoding genetic information by accurately joining amino acids to their cognate transfer RNAs. Here, tRNA discrimination at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding, analytical ultracentrifugation, and iodine fo...

journal_title：Biochemistry

authors： Bovee ML,Yan W,Sproat BS,Francklyn CS

更新日期：1999-10-12 00:00:00

abstract：:Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...

journal_title：Biochemistry

authors： Schinagl A,Kerschbaumer RJ,Sabarth N,Douillard P,Scholz P,Voelkel D,Hollerweger JC,Goettig P,Brandstetter H,Scheiflinger F,Thiele M

更新日期：2018-03-06 00:00:00

abstract：:Photolyzed rhodopsin is phosphorylated at multiple serine and threonine residues during the quenching of phototransduction. Sites of phosphorylation by rhodopsin kinase have been localized to the C-terminal region of rhodopsin, but no information was available on the kinetics and identity of phosphorylated residues. T...

journal_title：Biochemistry

authors： Ohguro H,Palczewski K,Ericsson LH,Walsh KA,Johnson RS

更新日期：1993-06-01 00:00:00

abstract：:Structure and dynamism in a DNA segment d-GATCTTCCCCCCGGAA have been investigated by nuclear magnetic resonance (NMR) spectroscopy. At neutral pH, the molecule exists largely as a dumbbell, formed by the association of two hairpins with sticky ends. A small percentage of hairpin is also detectable. The stem of the dum...

journal_title：Biochemistry

authors： Singh S,Patel PK,Hosur RV

更新日期：1997-10-28 00:00:00

abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...

journal_title：Biochemistry

authors： Stivers JT,Jagadeesh GJ,Nawrot B,Stec WJ,Shuman S

更新日期：2000-05-09 00:00:00

abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...

journal_title：Biochemistry

authors： Cocco MJ,Kao YH,Phillips AT,Lecomte JT

更新日期：1992-07-21 00:00:00

abstract：:The receptor for atrial natriuretic peptide (ANP) is a type-I transmembrane protein containing an extracellular ligand-binding domain, a single transmembrane sequence, an intracellular kinase-homologous domain, and a guanylate cyclase (GCase) domain. Binding of ANP to the extracellular domain causes activation of the ...

journal_title：Biochemistry

authors： Misono KS,Sivasubramanian N,Berkner K,Zhang X

更新日期：1999-01-12 00:00:00

abstract：:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...

journal_title：Biochemistry

authors： Fewtrell C,Kempner E,Poy G,Metzger H

更新日期：1981-11-10 00:00:00

abstract：:Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...

journal_title：Biochemistry

authors： Graves PE,Kaminsky LS,Halpert J

更新日期：1987-06-30 00:00:00