Structural polymorphism and dynamism in the DNA segment GATCTTCCCCCCGGAA: NMR investigations of hairpin, dumbbell, nicked duplex, parallel strands, and i-motif.

abstract：:The distribution of one-disulfide bonds in the two-disulfide intermediates in the oxidative refolding of bovine pancreatic ribonuclease A has been characterized. These two-disulfide intermediates were formed from the fully reduced denatured protein by oxidation with dithiothreitol, then blocked with AEMTS, purified by...

journal_title：Biochemistry

authors： Volles MJ,Xu X,Scheraga HA

更新日期：1999-06-01 00:00:00

abstract：:A rapid assay procedure was developed for cleavage of the N-terminal propeptides of procollagen. With the assay a neutral procollagen N-protease was purified about 300-fold from chick embryo tendon extract. The enzyme had an apparent molecular weight of 260 000 and a pH optimum of 7.4. Ca2+ was required for enzymic ac...

journal_title：Biochemistry

authors： Tuderman L,Kivirikko KI,Prockop DJ

更新日期：1978-07-25 00:00:00

abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

journal_title：Biochemistry

authors： Yamagami R,Huang R,Bevilacqua PC

更新日期：2019-09-24 00:00:00

abstract：:The incorporation of 7-dehydrocholesterol into synthetic phospholipid bilayers altered the distribution of products after photolysis. In liposomes, the relative amounts of 7-dehydrocholesterol and lumisterol were elevated, and tachysterol was reduced from the levels observed in hexane solution. Z to E isomerization of...

journal_title：Biochemistry

authors： Yamamoto JK,Borch RF

更新日期：1985-06-18 00:00:00

abstract：:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...

journal_title：Biochemistry

authors： Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JM

更新日期：1993-12-14 00:00:00

abstract：:S-Protein/vitronectin is a serum glycoprotein that inhibits the lytic activity of the membrane attack complex of complement, i.e., of the complex including the proteins C5b, C6, C7, C8, and C9n. We show that intact S-protein/vitronectin or its cyanogen bromide generated fragments also inhibit the hemolysis mediated by...

journal_title：Biochemistry

authors： Tschopp J,Masson D,Schäfer S,Peitsch M,Preissner KT

更新日期：1988-05-31 00:00:00

abstract：:We report a straightforward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity and inhibition of fucosyltransferase VIII, which catal...

journal_title：Biochemistry

authors： Soroko M,Kwan DH

更新日期：2020-06-09 00:00:00

abstract：:Much interest is currently focused on understanding the detailed contribution that particular amino acid residues make in protein structure and function. Although the use of site-directed mutagenesis has greatly contributed to this goal, the approach is limited to the standard repertoire of twenty amino acids. Fluorin...

journal_title：Biochemistry

authors： Duewel H,Daub E,Robinson V,Honek JF

更新日期：1997-03-18 00:00:00

abstract：:Two antisera used in the radioimmunoassay for human fibrinopeptide A (FPA) which appear to have different immunochemical specificities have been tested for cross-reactivity with fibrinogen and with three fragments of fibrinogen which contain the FPA sequence. These fragments were the three-chain, NH2-terminal disulfid...

journal_title：Biochemistry

authors： Canfield RE,Dean J,Nossel HL,Butler VP Jr,Wilner GD

更新日期：1976-03-23 00:00:00

abstract：:The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...

journal_title：Biochemistry

authors： Weber PL,Wemmer DE,Reid BR

更新日期：1985-08-13 00:00:00

abstract：:A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...

journal_title：Biochemistry

authors： Whiting AK,Boldt YR,Hendrich MP,Wackett LP,Que L Jr

更新日期：1996-01-09 00:00:00

abstract：:Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...

journal_title：Biochemistry

authors： Stoychev SH,Nathaniel C,Fanucchi S,Brock M,Li S,Asmus K,Woods VL Jr,Dirr HW

更新日期：2009-09-08 00:00:00

abstract：:The effect of protonation events on the charge equilibrium between tyrosine-D and the water-oxidizing complex in photosystem II has been studied by time-resolved measurements of the EPR signal IIslow at room temperature. The flash-induced oxidation of YD by the water-oxidizing complex in the S2 state is a monophasic p...

journal_title：Biochemistry

authors： Vass I,Styring S

更新日期：1991-01-22 00:00:00

abstract：:Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational ensemble of the tandemly repeating tetrapeptide unit of the circumsporozoite coat protein of the malaria parasite Plasmodium falciparum. Peptides based on the Asn-Ala-Asn-Pro and Asn-Pro-Asn-Ala...

journal_title：Biochemistry

authors： Dyson HJ,Satterthwait AC,Lerner RA,Wright PE

更新日期：1990-08-28 00:00:00

abstract：:We have examined the C cluster in type II CO dehydrogenase (CODH) from Carboxydothermus hydrogenformans using Ni K-edge X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure (EXAFS) spectroscopy. The enzyme was studied under three conditions: "as-isolated" and after treatment with CO or ...

journal_title：Biochemistry

authors： Gu W,Seravalli J,Ragsdale SW,Cramer SP

更新日期：2004-07-20 00:00:00

abstract：:Globotriaosylceramide [(Gal alpha 1-4Gal beta 1-4Glc-ceramide (Gb3)] was separated from human kidney, and the fatty acid composition was determined. Semisynthetic Gb3 molecular species of corresponding fatty acid chain length were prepared and compared for verotoxin (VT) binding affinity by TLC overlay, and a quantita...

journal_title：Biochemistry

authors： Pellizzari A,Pang H,Lingwood CA

更新日期：1992-02-11 00:00:00

abstract：:NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active asse...

journal_title：Biochemistry

authors： Barbier P,Peyrot V,Leynadier D,Andreu JM

更新日期：1998-01-13 00:00:00

abstract：:By using targeted disulfide cross-linking, we have characterized structural changes that the Escherichia coli aspartate receptor undergoes upon modification of the four specific residues that are reversibly methylated during sensory adaptation. Cysteine residues were introduced at specific positions either in the cyto...

journal_title：Biochemistry

authors： Le Moual H,Quang T,Koshland DE Jr

更新日期：1998-10-20 00:00:00

abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...

journal_title：Biochemistry

authors： Kong BY,Clarke RJ

更新日期：2004-03-02 00:00:00

abstract：:The effect of substituting Pro-L209 with Tyr, Phe, Glu, and Thr in photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides was investigated by monitoring the light-induced FTIR absorption changes associated with the photoreduction of the secondary quinone Q(B). Pro-L209 is close to a chain of ordered water ...

journal_title：Biochemistry

authors： Nabedryk E,Breton J,Sebban P,Baciou L

更新日期：2003-05-20 00:00:00

abstract：:In eukaryotes, choline acetyltransferase (ChAT) catalyzes the reversible formation of the neurotransmitter acetylcholine from choline and acetyl-CoA. ChAT belongs to a family of CoA-dependent enzymes that also includes the carnitine acyltransferases CrAT, CrOT, and CPTs. In contrast to CrOT and CPTs that are very acti...

journal_title：Biochemistry

authors： Green KD,Porter VR,Zhang Y,Garneau-Tsodikova S

更新日期：2010-07-27 00:00:00

abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

journal_title：Biochemistry

authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

更新日期：2007-03-13 00:00:00

abstract：:DNA gyrase is the target of a number of antibacterial agents, including the coumarins and the cyclothialidines. To extend our understanding of the mechanism of action of these compounds, we have examined the previously published crystal structures of the complexes between the 24 kDa fragment of GyrB and coumarin and c...

journal_title：Biochemistry

authors： Kampranis SC,Gormley NA,Tranter R,Orphanides G,Maxwell A

更新日期：1999-02-16 00:00:00

abstract：:The type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri , providing a conduit through which host-altering effectors are injected directly into a host cell to promote uptake. The type III secretion apparatus (T3SA) is composed of a basal body, external needle, and regulatory tip comp...

journal_title：Biochemistry

authors： Dickenson NE,Arizmendi O,Patil MK,Toth RT 4th,Middaugh CR,Picking WD,Picking WL

更新日期：2013-12-10 00:00:00

abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...

journal_title：Biochemistry

authors： Polacow I,Cabasso L,Li HJ

更新日期：1976-10-19 00:00:00

abstract：:Preferential interaction of trans-membrane alpha-helices whose hydrophobic length matches the hydrophobic thickness of the lipid bilayer could be a mechanism of retention in the Golgi apparatus. We have used fluorescence methods to study the interaction of peptides Ac-K2-G-Lm-W-Ln-K2-A-amide (Pm+n) with bilayers of ph...

journal_title：Biochemistry

authors： Webb RJ,East JM,Sharma RP,Lee AG

更新日期：1998-01-13 00:00:00

abstract：:Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...

journal_title：Biochemistry

authors： Yanchunas J Jr,Dabrowski MJ,Schurke P,Atkins WM

更新日期：1994-12-20 00:00:00

abstract：:The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and beta-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain. Two mutants were crea...

journal_title：Biochemistry

authors： Scott SP,Jarjous S

更新日期：2005-06-21 00:00:00

abstract：:The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or tr...

journal_title：Biochemistry

authors： Rudnick G,Nelson PJ

更新日期：1978-11-28 00:00:00

abstract：:The acid-base properties of ionophore A23187 in methanol-water solutions (0--95% w/w) and bound to unilamellar vesicles of dimyristoylphosphatidylcholine were examined by ultraviolet and fluorescence spectroscopy, and the spectral properties for the acidic and basic forms were defined under these conditions. Standard ...

journal_title：Biochemistry

authors： Kauffman RF,Taylor RW,Pfeiffer DR

更新日期：1982-05-11 00:00:00