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The histone binding protein nucleoplasmin does not facilitate binding of transcription factor IIIA to nucleosomal Xenopus laevis 5S rRNA genes.

Abstract：

:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previously reported nucleosome remodeling ability of nucleoplasmin, the binding of TFIIIA to nucleosomal DNA cannot be facilitated by this protein. Furthermore, it was demonstrated that nucleoplasmin cannot overcome nucleosome mediated repression of transcription of reconstituted 5S rRNA genes. In contrast to earlier work, this study used a homologous system composed of the 5S rRNA gene, nucleoplasmin, and TFIIIA from Xenopus laevis.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Howe L,Itoh T,Katagiri C,Ausio J

doi

10.1021/bi972723+

subject

Has Abstract

pub_date

1998-02-03 00:00:00

pages

1174-7

issue

5

eissn

0006-2960

issn

1520-4995

pii

bi972723+

journal_volume

37

pub_type

杂志文章

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