Sequential phosphorylation of rhodopsin at multiple sites.

abstract：:The conformational conversion of prion protein (PrP) from an alpha-helix-rich normal cellular isoform (PrPC) to a beta-sheet-rich pathogenic isoform (PrPSc) is a key event in the development of prion diseases, and it takes place in caveolae, cavelike invaginations of the plasma membrane. A peptide homologous to residu...

journal_title：Biochemistry

authors： Miura T,Yoda M,Takaku N,Hirose T,Takeuchi H

更新日期：2007-10-16 00:00:00

abstract：:Thiazolinyl imine reductases catalyze the NADPH-dependent reduction of a thiazoline to a thiazolidine, a required step in the formation of the siderophores yersiniabactin (Yersinia spp.) and pyochelin (Pseudomonas aeruginosa). These stand-alone nonribosomal peptide tailoring domains are structural homologues of sugar ...

journal_title：Biochemistry

authors： Meneely KM,Ronnebaum TA,Riley AP,Prisinzano TE,Lamb AL

更新日期：2016-09-27 00:00:00

abstract：:Peptides have important biomedical applications, but poor correlation between in vitro and in vivo activities can limit their development for clinical use. The ability to generate peptides and monitor their expression with new mass spectrometric methods and biological activities in vivo would be an advantage for the d...

journal_title：Biochemistry

authors： Muthunayake NS,Islam R,Inutan ED,Colangelo W,Trimpin S,Cunningham PR,Chow CS

更新日期：2020-09-15 00:00:00

abstract：:Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...

journal_title：Biochemistry

authors： Kobayashi Y,Tsutsumi H,Abe T,Ikeda K,Tashiro Y,Unzai S,Kamikubo H,Kataoka M,Hiroaki H,Hamada D

更新日期：2014-08-12 00:00:00

abstract：:Little is known about the extent to which protein flexibility contributes to antigen-antibody recognition and cross-reactivity. Using short coil peptides (leucine zippers) as model antigens, we demonstrate that a monoclonal antibody can force a noncognate peptide into a conformation that is similar to the conformation...

journal_title：Biochemistry

authors： Leder L,Berger C,Bornhauser S,Wendt H,Ackermann F,Jelesarov I,Bosshard HR

更新日期：1995-12-19 00:00:00

abstract：:The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...

journal_title：Biochemistry

authors： Guimarães SL,Coitinho JB,Costa DM,Araújo SS,Whitman CP,Nagem RA

更新日期：2016-05-10 00:00:00

abstract：:The reactivity of the mitochondrial (pI = 5) isoenzyme of horse liver aldehyde dehydrogenase was determined by studying the effects of pH on steady-state velocity, burst magnitude, and molecular weight of the enzyme in the absence and presence of Mg2+ ions. The Mg2+ ion activation of the steady-state velocity at pH 7....

journal_title：Biochemistry

authors： Takahashi K,Weiner H,Filmer DL

更新日期：1981-10-13 00:00:00

abstract：:The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....

journal_title：Biochemistry

authors： Grasser M,Christensen JM,Peterhänsel C,Grasser KD

更新日期：2007-05-29 00:00:00

abstract：:Earlier thermodynamic studies of the intermolecular interactions between mature oxytocin and neurophysin, and of the effects of these interactions on neurophysin folding, raised questions about the intramolecular interactions of oxytocin with neurophysin within their common precursor. To address this issue, the disulf...

journal_title：Biochemistry

authors： Eubanks S,Lu M,Peyton D,Breslow E

更新日期：1999-10-12 00:00:00

abstract：:The structural factors responsible for the extraordinary rate enhancement ( approximately 10(17)) of the reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) have not been defined. Catalysis requires a conformational change that closes an active site loop and "clamps" the orotate base proximal to hyd...

journal_title：Biochemistry

authors： Wood BM,Amyes TL,Fedorov AA,Fedorov EV,Shabila A,Almo SC,Richard JP,Gerlt JA

更新日期：2010-05-04 00:00:00

abstract：:We propose a simple method to find an amino acid sequence that is foldable into a globular protein with a desired structure based on a knowledge-based 3D-1D compatibility function. An asymmetric alpha-helical single-domain structure of sperm whale myoglobin consisting of 153 amino acid residues was chosen for the desi...

journal_title：Biochemistry

authors： Isogai Y,Ota M,Fujisawa T,Izuno H,Mukai M,Nakamura H,Iizuka T,Nishikawa K

更新日期：1999-06-08 00:00:00

abstract：:Iron is vital for almost all organisms because of its ability to donate and accept electrons with relative ease. It serves as a cofactor for many proteins and enzymes necessary for oxygen and energy metabolism, as well as for several other essential processes. Mammalian cells utilize multiple mechanisms to acquire iro...

journal_title：Biochemistry

authors： Pantopoulos K,Porwal SK,Tartakoff A,Devireddy L

更新日期：2012-07-24 00:00:00

abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...

journal_title：Biochemistry

authors： Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

更新日期：1998-04-28 00:00:00

abstract：:S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...

journal_title：Biochemistry

authors： Bale S,Brooks W,Hanes JW,Mahesan AM,Guida WC,Ealick SE

更新日期：2009-07-14 00:00:00

abstract：:The three lipoyl (E2plip) domains of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase (PDH) complex of Escherichia coli house the lipoyl-lysine side chain essential for active-site coupling and substrate channelling within the complex. The structure of the unlipoylated form of the innermost ...

journal_title：Biochemistry

authors： Jones DD,Stott KM,Howard MJ,Perham RN

更新日期：2000-07-25 00:00:00

abstract：:The synthesis of 5-(2-oxalylethyl)-NADH, a reduced nicotinamide adenine dinucleotide (NADH) derivate with pyruvate covalently attached to the 5 position of the dihydronicotinamide ring over an additional methylene group has been described previously (Trommer, W.E., Blume, H., and Kapmeyer, H. (1976) Justus Liebigs Ann...

journal_title：Biochemistry

authors： Kapmeyer H,Pfleiderer G,Trommer WE

更新日期：1976-11-16 00:00:00

abstract：:Sufiredoxins (Srx) repair the inactivated forms of typical two-Cys peroxiredoxins (Prx) implicated in hydrogen peroxide-mediated cell signaling. The reduction of the cysteine sulfinic acid moiety within the active site of the Prx by Srx involves novel sulfur chemistry and the use of ATP and Mg(2+). The 1.65 A crystal ...

journal_title：Biochemistry

authors： Jönsson TJ,Murray MS,Johnson LC,Poole LB,Lowther WT

更新日期：2005-06-21 00:00:00

abstract：:The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...

journal_title：Biochemistry

authors： Lee H,Angus RH,Storer AC,Varughese KI,Carey PR

更新日期：1988-01-12 00:00:00

abstract：:Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...

journal_title：Biochemistry

authors： Wiktorowicz JE,Stafford S,Rea H,Urvil P,Soman K,Kurosky A,Perez-Polo JR,Savidge TC

更新日期：2011-06-28 00:00:00

abstract：:The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...

journal_title：Biochemistry

authors： Ritter M,Anderka O,Ludwig B,Mäntele W,Hellwig P

更新日期：2003-10-28 00:00:00

abstract：:Cadherins are single pass transmembrane glycoproteins which mediate calcium dependent cell-cell adhesion by homophilic interactions. To reveal the molecular details of calcium binding and homoassociation, we recombinantly expressed in Escherichia coli a domain pair consisting of the first two domains of E-cadherin (EC...

journal_title：Biochemistry

authors： Koch AW,Pokutta S,Lustig A,Engel J

更新日期：1997-06-24 00:00:00

abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...

journal_title：Biochemistry

authors： Dai Y,Pochapsky TC,Abeles RH

更新日期：2001-05-29 00:00:00

abstract：:Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

journal_title：Biochemistry

authors： Aitken SM,Kim DH,Kirsch JF

更新日期：2003-09-30 00:00:00

abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

journal_title：Biochemistry

authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

更新日期：1994-09-20 00:00:00

abstract：:The effect of mutating Gly 478 to Ala in rat cytochrome P450 2B1 on the metabolism of N-benzyl-1-aminobenzotriazole was investigated. The 7-ethoxy-4-(trifluoromethyl)coumarin O-deethylation activity of the wild-type enzyme was completely inactivated by incubating with 1 microM BBT. The G478A mutant, however, was not i...

journal_title：Biochemistry

authors： Kent UM,Hanna IH,Szklarz GD,Vaz AD,Halpert JR,Bend JR,Hollenberg PF

更新日期：1997-09-30 00:00:00

abstract：:The thermodynamic parameters characterizing the interaction between rabbit fast skeletal muscle troponin and tropomyosin have been determined at 25 degrees C for three solution conditions: buffer containing (A) 1 mM CaCl2, simulating a "turned-on" state, (B) 3 mM MgCl2, simulating a "turned-off" state, and (C) 2 mM et...

journal_title：Biochemistry

authors： Ingraham RH,Swenson CA

更新日期：1985-09-10 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:To gain insight into the folding of large proteins, we constructed a bovine beta-lactoglobulin (beta-lg) dimeric mutant, A34C/C121A beta-lg. In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer's inte...

journal_title：Biochemistry

authors： Yagi M,Kameda A,Sakurai K,Nishimura C,Goto Y

更新日期：2008-06-03 00:00:00

abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：1999-03-09 00:00:00

abstract：:Structural changes during the photochemical reactions of unhydrated air-dried films of bovine rhodopsin in rod outer segments were examined by visible and Fourier transform infrared (FTIR) spectroscopy at 200, 240, and 280 K. These films exhibited conversion from a lumirhodopsin state to a metarhodopsin I state with a...

journal_title：Biochemistry

authors： Nishimura S,Sasaki J,Kandori H,Lugtenburg J,Maeda A

更新日期：1995-12-26 00:00:00