Abstract:
:Cadherins are single pass transmembrane glycoproteins which mediate calcium dependent cell-cell adhesion by homophilic interactions. To reveal the molecular details of calcium binding and homoassociation, we recombinantly expressed in Escherichia coli a domain pair consisting of the first two domains of E-cadherin (ECAD12) and the single domains 1, 2, and 5. ECAD12 encompasses the most N-terminal of the four putative calcium-binding pockets in the extracellular region of E-cadherin. Equilibrium dialysis experiments revealed that the single domains do not bind Ca2+, but ECAD12 was found to bind three calcium ions. ECAD12 dimerizes (Kd = 0.08 +/- 0.02 mM) in the presence of Ca2+ as we could demonstrate by analytical ultracentrifugation. Calcium binding to ECAD12 induces conformational changes which were monitored by electrophoretic mobility and by circular dichroism. By analyzing our equilibrium dialysis data with a single binding site model, we found an average Kd of 460 microM for the three bound Ca2+. Assuming a model for three binding sites, which slightly increased the quality of the fit, we obtained two identical Kds of 330 microM and a third much higher Kd of 2 mM. The entire extracellular region of E-cadherin, which was recombinantly expressed in mammalian cells, binds nine Ca2+ with a much lower average Kd of 30 microM. Therefore, we conclude that the four calcium binding pockets are not identical. Since binding to ECAD12 occurs at Ca2+ concentrations close to those in the extracellular space, we suggest that the N-terminal domain pair might be involved in calcium regulation of E-cadherin mediated cell-cell adhesion.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Koch AW,Pokutta S,Lustig A,Engel Jdoi
10.1021/bi9705624subject
Has Abstractpub_date
1997-06-24 00:00:00pages
7697-705issue
25eissn
0006-2960issn
1520-4995pii
bi9705624journal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00140a024
更新日期:1992-06-30 00:00:00
abstract::SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural differences...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00977
更新日期:2015-11-24 00:00:00
abstract::Nonribosomal peptide synthetases (NRPSs) are megasynthetases that require complex and specific interactions between multiple domains and proteins to functionally produce a metabolite. MbtH-like proteins (MLPs) are integral components of many NRPSs and interact directly with the adenylation domain of the megasynthetase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00552
更新日期:2018-07-17 00:00:00
abstract::Fom3, the antepenultimate enzyme in the fosfomycin biosynthetic pathway in Streptomyces spp., is a class B cobalamin-dependent radical SAM methyltransferase that catalyzes methylation of (5'-cytidylyl)-2-hydroxyethylphosphonate (2-HEP-CMP) to form (5'-cytidylyl)-2-hydroxypropylphosphonate (2-HPP-CMP). Previously, the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00616
更新日期:2018-08-21 00:00:00
abstract::Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400652c
更新日期:2013-07-16 00:00:00
abstract::Pyruvate dehydrogenase kinase 2 (PDK2) activity is enhanced by the dihydrolipoyl acetyltransferase core (E2 60mer) that binds PDK2 and a large number of its pyruvate dehydrogenase (E1) substrate. With E2-activated PDK2, K(+) at approximately 90 mM and Cl(-) at approximately 60 mM decreased the K(m) of PDK2 for ATP and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049488x
更新日期:2004-10-26 00:00:00
abstract::alpha 1-Adrenergic receptor probes, which can be radioiodinated to yield high specific activity radioligands, have been synthesized and characterized. 2-[4-(4-Amino-benzoyl)piperazin-1-yl]-4-amino-6,7-dimethoxyquin azoline (CP63,155), an arylamine analogue of the selective alpha 1-adrenergic antagonist prazosin, and i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00311a031
更新日期:1984-07-31 00:00:00
abstract::Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00114a016
更新日期:1991-12-17 00:00:00
abstract::Tyr71 is an invariant residue in all known sequences of tyrosine phenol-lyase (TPL). The substitution of Tyr71 in TPL by phenylalanine results in a mutant Y71F TPL with no detectable activity (greater than 3 x 10(5)-fold reduction) for beta-elimination of L-tyrosine. Y71F TPL can react with S-alkylcysteines, but these...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00038a023
更新日期:1995-09-26 00:00:00
abstract::The reversible inhibition of beta-lactamase I from Bacillus cereus by cloxacillin, methicillin, and nafcillin has been systematically investigated. For these substrates the enzymatic reaction involves partitioning of the substrate between turnover and inhibition. Typically, concentrations of several hundred millimolar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00388a011
更新日期:1987-07-14 00:00:00
abstract::A peptide consisting of the first 21 residues of human myelin basic protein (MBP) was synthesized. The N-terminal alanine of portions was blocked in separate experiments with alkyl carboxylates varying in size from 2 to 10 carbon atoms. The effects of these different alkyl carboxylates at the N-terminus on the seconda...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00180a008
更新日期:1994-04-12 00:00:00
abstract::Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048201t
更新日期:2005-03-08 00:00:00
abstract::The interaction of profilin and non-muscle beta,gamma-actin prepared from bovine spleen has been investigated under physiologic ionic conditions. Profilin binding to actin decreases the affinity of actin for MgADP and MgATP by about 65- and 13-fold, respectively. Kinetic measurements indicate that profilin binding to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001520+
更新日期:2000-10-31 00:00:00
abstract::Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049017a
更新日期:2004-12-28 00:00:00
abstract::The N-terminal 17% of apolipoprotein B (apoB-17) is secreted lipid-poor while apoB-41 particles are secreted with a triacylglycerol (TAG)-rich core. Thus, the sequence between apoB-17 and apoB-41 is necessary for the assembly of TAG-rich lipoproteins. To delineate this region, C127 cells were permanently transfected t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000791h
更新日期:2000-08-15 00:00:00
abstract::The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00177a016
更新日期:1994-03-22 00:00:00
abstract::Vc H-NOX (or VCA0720) is an H-NOX (heme-nitric oxide and oxygen binding) protein from facultative aerobic bacterium Vibrio cholerae. It shares significant sequence homology with soluble guanylyl cyclase (sGC), a NO sensor protein commonly found in animals. Similar to sGC, Vc H-NOX binds strongly to NO and CO with affi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401408x
更新日期:2013-12-31 00:00:00
abstract::In reconstituted vesicles above the lipid phase transition temperature, bacteriorhodopsin (BR) undergoes rotational diffusion about an axis perpendicular to the plane of the bilayer [Cherry, R. J., Muller, U., & Schneider, G. (1977) FEBS Lett. 80, 465]. This diffusion narrows the 13C NMR powder line shape of the BR pe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00338a029
更新日期:1985-08-13 00:00:00
abstract::AppA, a transcriptional antirepressor, regulates the steady expression of photosynthesis genes in Rhodobacter sphaeroides in response to high-intensity blue light and to redox signals. Its blue-light sensing is mediated by an N-terminal BLUF domain, a member of a novel flavin fold. The photocycle of this domain (AppA(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051367p
更新日期:2006-01-10 00:00:00
abstract::The backbone dynamics of Escherichia coli ribonuclease HI (RNase HI) in the picosecond to nanosecond time scale were characterized by a combination of measurements of 15N-NMR relaxation (T1, T2, and NOE), analyzed by a model-free approach, and molecular dynamics (MD) simulation in water. The MD simulations in water we...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00020a003
更新日期:1995-05-23 00:00:00
abstract::Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resist...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971276v
更新日期:1997-10-21 00:00:00
abstract::Catalase (hydroperoxidase) HPII of Escherichia coli is the largest catalase so far characterized, existing as a homotetramer of 84 kDa subunits. Each subunit has a core structure that closely resembles small subunit catalases, supplemented with an extended N-terminal sequence and compact flavodoxin-like C-terminal dom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034208j
更新日期:2003-05-20 00:00:00
abstract::During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700416h
更新日期:2007-07-10 00:00:00
abstract::Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061722r
更新日期:2007-03-20 00:00:00
abstract::Conceptually one may envisage that substrate binding sites on the ABC transporter P-gp cycle between high- and low-affinity conformations in response to signals arising from nucleotide hydrolysis to effect active transport. A radioligand binding assay was used to characterize the interaction of [3H]vinblastine with P-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011211z
更新日期:2001-12-25 00:00:00
abstract::A receptor binding region of mouse interferon gamma (IFN gamma) has previously been localized to the N-terminal 39 amino acids of the molecule by use of synthetic peptides and monoclonal antibodies. In this report, a detailed analysis of the synthetic peptide corresponding to this region, IFN gamma (1-39), is presente...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00237a022
更新日期:1991-06-11 00:00:00
abstract::hTom20 is an outer mitochondrial membrane receptor involved in protein translocation. The cytosolic domain (aa30-145) and selected truncated versions of this domain were overexpressed and purified to study the structure-function relationship of this protein. Our studies reveal that the secondary structure of the cytos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980746y
更新日期:1998-09-22 00:00:00
abstract::Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. The heme binding region has been localized to residues 1-385 of the beta1 subunit [beta1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972686m
更新日期:1998-03-31 00:00:00
abstract::The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990507q
更新日期:1999-11-02 00:00:00
abstract::In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961360v
更新日期:1996-12-10 00:00:00