Crystal structures of the Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase-GMP and -IMP complexes: comparison of purine binding interactions with the XMP complex.

abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...

journal_title：Biochemistry

authors： Owen P,Kaback HR

更新日期：1979-04-17 00:00:00

abstract：:Indolicidin is an antimicrobial cationic peptide with broad-spectrum activity isolated from bovine neutrophils. An indolicidin analogue CP-11, ILKKWPWWPWRRK-NH(2), with improved activity against Gram-negative bacteria had increased positive charge and amphipathicity while maintaining the short length of the parent mol...

journal_title：Biochemistry

authors： Rozek A,Powers JP,Friedrich CL,Hancock RE

更新日期：2003-12-09 00:00:00

abstract：:Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue wit...

journal_title：Biochemistry

authors： Mchaourab HS,Lietzow MA,Hideg K,Hubbell WL

更新日期：1996-06-18 00:00:00

abstract：:An endogenous calcium-activated sulfhydryl protease in chick oviduct cytosol has been utilized to study the structure of the chick oviduct progesterone receptor subunits, progestophilins A (79 000 g/mol) and B (117 000 g/mol). The protease is not a normal component of the native progesterone receptor aggregate (6 and ...

journal_title：Biochemistry

authors： Vedeckis WV,Schrader WT,O'Malley BW

更新日期：1980-01-22 00:00:00

abstract：:Nucleic acid aptamers are rapidly gaining prominence as diagnostic tools, targeting reagents, and potential therapeutics. To extend the use of aptamers into the biochemical analysis of protein interactions on the surface of live cells, we converted an enzymatically generated RNA aptamer into a photo-cross-linkable aff...

journal_title：Biochemistry

authors： Park E,Baron R,Landgraf R

更新日期：2008-11-18 00:00:00

abstract：:Kinetics of flash-induced electron transfer from the soluble cytochrome c2 to the primary donor (P) of the reaction center purified from the purple bacterium Rhodobacter sphaeroides R-26 were investigated by time-resolved absorption spectroscopy. Re-reduction of P+ induced by a laser pulse was measured at 1283 nm both...

journal_title：Biochemistry

authors： Venturoli G,Mallardi A,Mathis P

更新日期：1993-12-07 00:00:00

abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...

journal_title：Biochemistry

authors： Kikkawa S,Tominaga K,Nakagawa M,Iwasa T,Tsuda M

更新日期：1996-12-10 00:00:00

abstract：:The site-directed mutagenesis of a number of proposed active site residues of 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase is reported. Several of these mutations resulted in complete loss of enzyme activity indicating that these residues are probably involved with catalysis, notably K22R, K411R, D384A, R27A, R...

journal_title：Biochemistry

authors： Shuttleworth WA,Pohl ME,Helms GL,Jakeman DL,Evans JN

更新日期：1999-01-05 00:00:00

abstract：:FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...

journal_title：Biochemistry

authors： Hou C,Tsodikov OV

更新日期：2015-12-22 00:00:00

abstract：:The purL gene of Escherichia coli encoding the enzyme formylglycinamidine ribonucleotide (FGAM) synthetase which catalyzes the conversion of formylglycinamide ribonucleotide (FGAR), glutamine, and MgATP to FGAM, glutamate, ADP, and Pi has been cloned and sequenced. The mature protein, as deduced by the structural gene...

journal_title：Biochemistry

authors： Schendel FJ,Mueller E,Stubbe J,Shiau A,Smith JM

更新日期：1989-03-21 00:00:00

abstract：:Extended-spectrum β-lactamases (ESBLs) pose a threat to public health because of their ability to confer resistance to extended-spectrum cephalosporins such as cefotaxime. The CTX-M β-lactamases are the most widespread ESBL enzymes among antibiotic resistant bacteria. Many of the active site residues are conserved bet...

journal_title：Biochemistry

authors： Adamski CJ,Cardenas AM,Brown NG,Horton LB,Sankaran B,Prasad BV,Gilbert HF,Palzkill T

更新日期：2015-01-20 00:00:00

abstract：:We show that eight different serine proteinases--bovine chymotrypsins A and B, porcine pancreatic elastase I, proteinase K, Streptomyces griseus proteinases A and B, and subtilisins BPN' and Carlsberg--interact with turkey ovomucoid third domain at the same Leu18-Glu19 peptide bond, the reactive site of the inhibitor....

journal_title：Biochemistry

authors： Ardelt W,Laskowski M Jr

更新日期：1985-09-24 00:00:00

abstract：:Circular dichroism and difference spectroscopy have been used to study dilute aqueous solutions of oxygenated, deoxygenated, and carbamoylated deoxygenated hemoglobins A and S (HbA and HbS, respectively). The spectra of HbA and HbS, in comparable state of oxygenation or carbamoylation, are identical, strongly implying...

journal_title：Biochemistry

authors： Simons ER,Hartzband P,Whitin J,Chapman C

更新日期：1976-09-07 00:00:00

abstract：:Numerous studies have concluded that the interaction of the amyloid β-peptide (Aβ) and cellular membranes contributes to the toxicity and cell death observed in the progression of Alzheimer's disease. Aggregated Aβ species disrupt membranes, leading to physical instability and ion leakage. Further, the presence of Aβ ...

journal_title：Biochemistry

authors： Lemkul JA,Bevan DR

更新日期：2013-07-23 00:00:00

abstract：:To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142),...

journal_title：Biochemistry

authors： Gorshkova IN,Liu T,Kan HY,Chroni A,Zannis VI,Atkinson D

更新日期：2006-01-31 00:00:00

abstract：:Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...

journal_title：Biochemistry

authors： Sugiki T,Furuita K,Fujiwara T,Kojima C

更新日期：2018-07-03 00:00:00

abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...

journal_title：Biochemistry

authors： Willis LJ,Loehr TM

更新日期：1985-05-21 00:00:00

abstract：:Chemical derivatization studies have been directed at the sulfhydryl group of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme. Reaction with N-ethylmaleimide leads to progressive and parallel loss of both enzymic activity and coenzyme binding. Both functions are lost when 1 equiv of sulfhydryl is deriva...

journal_title：Biochemistry

authors： Latruffe N,Brenner SC,Fleischer S

更新日期：1980-11-11 00:00:00

abstract：:The contributions to thermostability of interactions within the beta-sheet region of rubredoxins (Rds) were investigated by examining proteins in which beta-strand sequences of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were interchanged....

journal_title：Biochemistry

authors： Eidsness MK,Richie KA,Burden AE,Kurtz DM Jr,Scott RA

更新日期：1997-08-26 00:00:00

abstract：:1,4-Dihydroxy-2-naphthoyl-coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes an intramolecular Claisen condensation involving two oxyanion intermediates in the biosynthetic pathway of menaquinone, an essential respiration electron transporter in many microorganisms. Here we report the finding that the DHNA-CoA product...

journal_title：Biochemistry

authors： Chen M,Jiang M,Sun Y,Guo ZF,Guo Z

更新日期：2011-07-05 00:00:00

abstract：:An aminoglycoside 3-acetyltransferase [AAC(3)], possibly a new isoenzymic species of the 3-N-acetyltransferase group, was purified to apparent homogeneity from a crude extract of Pseudomonas aeruginosa, a gentamicin-resistant clinical isolate. The method of purification was consecutive column chromatography--(i) gel f...

journal_title：Biochemistry

authors： Coombe RG,George AM

更新日期：1982-03-02 00:00:00

abstract：:IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...

journal_title：Biochemistry

authors： Pelton JG,Torchia DA,Meadow ND,Wong CY,Roseman S

更新日期：1991-10-15 00:00:00

abstract：:Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. The magnitude of the SPR spectral changes caused by light varies with pH in a manner par...

journal_title：Biochemistry

authors： Salamon Z,Wang Y,Brown MF,Macleod HA,Tollin G

更新日期：1994-11-22 00:00:00

abstract：:The active site of histidine decarboxylase (HDC) from Lactobacillus 30a contains a pyruvoyl cofactor sitting at the interface of two molecules in a trimer. Although exhibiting hyperbolic kinetics at pH 4.8, near its optimum, HDC is cooperative at pH 7.6, indicating that the units of the trimer communicate. A Hill plot...

journal_title：Biochemistry

authors： Pishko EJ,Robertus JD

更新日期：1993-05-11 00:00:00

abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...

journal_title：Biochemistry

authors： Meinild AK,Loo DD,Hirayama BA,Gallardo E,Wright EM

更新日期：2001-10-02 00:00:00

abstract：:F62 LOS of Neisseria gonorrhoeae consists of two major LOS components; the higher and smaller molecular weight (MW) components were recognized by MAbs 1-1-M and 3F11 respectively. Base-line separation of the two major oligosaccharide (OS) components from F62 LOS was achieved by Bio-Gel P-4 chromatography after dephosp...

journal_title：Biochemistry

authors： Yamasaki R,Bacon BE,Nasholds W,Schneider H,Griffiss JM

更新日期：1991-10-29 00:00:00

abstract：:The effect of ionic strength on enzyme activity and stability varies considerably between enzymes. Ionic strength is known to affect the catalytic activity of some alkaline phosphatases (APs), such as Escherichia coli AP, but how ions affect APs is debated. Here, we studied the effect of various ions on a cold-adapted...

journal_title：Biochemistry

authors： Hjörleifsson JG,Ásgeirsson B

更新日期：2017-09-26 00:00:00

abstract：:The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...

journal_title：Biochemistry

authors： Yakovlev S,Gorlatov S,Ingham K,Medved L

更新日期：2003-07-01 00:00:00

abstract：:The reduction of D-amino acid oxidase (DAAO) by hydrated electrons (eaq-) has been studied in the absence and presence of benzoate by pulse radiolysis. The eaq-did not reduce the flavin moiety in DAAO and reacted with the amino acid residues in the protein. In the presence of benzoate, eaq- first reacted with benzoate...

journal_title：Biochemistry

authors： Kobayashi K,Hirota K,Ohara H,Hayashi K,Miura R,Yamano T

更新日期：1983-04-26 00:00:00

abstract：:The substrate profiles for three uncharacterized enzymes (YcjM, YcjT, and YcjU) that are expressed from a cluster of 12 genes ( ycjM-W and ompG) of unknown function in Escherichia coli K-12 were determined. Through a comprehensive bioinformatic and steady-state kinetic analysis, the catalytic function of YcjT was dete...

journal_title：Biochemistry

authors： Mukherjee K,Narindoshvili T,Raushel FM

更新日期：2018-05-15 00:00:00