Abstract：
:In reconstituted vesicles above the lipid phase transition temperature, bacteriorhodopsin (BR) undergoes rotational diffusion about an axis perpendicular to the plane of the bilayer [Cherry, R. J., Muller, U., & Schneider, G. (1977) FEBS Lett. 80, 465]. This diffusion narrows the 13C NMR powder line shape of the BR peptide carbonyls. In contrast, BR in native purple membrane is relatively immobile and exhibits a rigid-lattice powder line shape. By use of the principal values of the rigid-lattice chemical shift tensor and the motionally narrowed line shape from the reconstituted system, the range of Euler angles of the leucine peptide groups relative to the diffusion axis has been calculated. The experimentally observed line shape is inconsistent with those expected for structures which consist entirely of either alpha helix or beta sheet perpendicular to the membrane or beta sheet tilted at angles up to about 60 degrees from the membrane normal. However, for two more complex structural models, the predicted line shapes agree well with the experimental one. These are, first, a structure consisting entirely of alpha1 helices tilted at 20 degrees from the membrane normal and, second, a combination of 60% alpha II helix perpendicular to the membrane plane and 40% antiparallel beta sheet tilted at 10-20 degrees from the membrane normal. The results also indicate that the peptide backbone of bacteriorhodopsin in native purple membrane is extremely rigid even at 40 degrees. The experiments presented here demonstrate a new approach, using solid-state nuclear magnetic resonance (NMR) methods, for structural studies of transmembrane proteins in fluid membrane environments, either natural or reconstituted.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lewis BA,Harbison GS,Herzfeld J,Griffin RGdoi
10.1021/bi00338a029subject
Has Abstractpub_date
1985-08-13 00:00:00pages
4671-9issue
17eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The three-dimensional structure of toxic shock syndrome toxin 1 (TSST-1) from Staphylococcus aureus has been determined and refined to an R value of 0.226 for data between 8- and 2.5-A resolution. Overall, the structure of TSST-1 is similar to that of another superantigen, staphylococcal enterotoxin B (SEB). The key d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00213a001
更新日期：1993-12-21 00:00:00
abstract：:To study the substrate specificity and mechanism of thioltransferase (TTase) catalysis, we have used 14C- and 35S-radiolabeled mixed disulfides of cysteine and glutathione (GSH) with various cysteine-containing proteins. These protein mixed disulfide substrates were incubated with glutathione, glutathione disulfide (G...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a021
更新日期：1993-04-06 00:00:00
abstract：:In synthetic biology, metabolic engineering, and gene therapy, there is a strong demand for orthogonal or externally controlled regulation of gene expression. Here, RNA-based regulatory devices represent a promising emerging alternative to proteins, allowing a fast and direct control of gene expression, as no synthesi...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.6b01218
更新日期：2017-03-07 00:00:00
abstract：:Aspartate-beta-semialdehyde dehydrogenase (ASADH) lies at the first branch point in the biosynthetic pathway through which bacteria, fungi, and the higher plants synthesize amino acids, including lysine and methionine and the cell wall component diaminopimelate from aspartate. Blocks in this biosynthetic pathway, whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015713o
更新日期：2001-12-04 00:00:00
abstract：:The presumed role of an overactive protein arginine deiminase 4 (PAD4) in the pathophysiology of rheumatoid arthritis (RA) suggests that PAD4 inhibitors could be used to treat an underlying cause of RA, potentially offering a mechanism to stop further disease progression. Thus, the development of such inhibitors is of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700095s
更新日期：2007-06-05 00:00:00
abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00123a006
更新日期：1992-03-03 00:00:00
abstract：:In the primary quinone (Q(A)) binding site of Rb. sphaeroides reaction centers (RCs), isoleucine M265 is in extensive van der Waals contact with the ubiquinone headgroup. Substitution of threonine or serine for this residue (mutants M265IT and M265IS), but not valine (mutant M265IV), lowers the redox midpoint potentia...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026958j
更新日期：2003-04-15 00:00:00
abstract：:Metallo-beta-lactamases are a newly characterized family of zinc enzymes present in several pathogenic strains that represent an emerging clinical threat. Enzymes from different organisms exhibit an outstanding functional diversity, particularly in the metal ion requirements for activity. We have investigated the effe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010933n
更新日期：2002-02-12 00:00:00
abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00109a020
更新日期：1991-11-12 00:00:00
abstract：:Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701886t
更新日期：2008-01-22 00:00:00
abstract：:RNA competent in directing protein synthesis is sequestered in unfertilized sea urchin eggs as translationally quiescent, nonpolyribosomal, messenger ribonucleoprotein complexes (mRNPs). Following fertilization, these mRNPs are derepressed and actively translated, presumably due to changes in the mRNA-associated prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00553a029
更新日期：1980-06-10 00:00:00
abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00028a022
更新日期：1995-07-18 00:00:00
abstract：:Both agonist- and antagonist-bound glucocorticoid receptors (GRs) and progesterone receptors (PRs) regulate gene transcription with the assistance of corepressors (NCoR and SMRT) and coactivators (TIF2/GRIP1, SRC1, and AIB1). Receptor binding of these cofactors is competitive and is considered to involve interactions ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7004575
更新日期：2007-07-10 00:00:00
abstract：:The binding of a ligand to cell surface receptors initiates a cascade of intracellular signals that generate responses to the external stimuli. Thus, this event plays a pivotal role in the mechanism of transmembrane signaling. Activin is a member of a cytokine family that is involved in diverse biological processes. T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981939o
更新日期：1998-11-24 00:00:00
abstract：:Coupling of bovine carboxypeptidase A with diazotized 5-amino-1H-tetrazole increases esterase activity, decreases peptidase activity slightly, and modifies one tyrosyl residue. Subsequent nitration of the azoenzyme has no further effect on esterase activity, decreases peptidase activity markedly, and modifies a second...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00603a005
更新日期：1978-05-16 00:00:00
abstract：:Bacteriophage P22 is a double-stranded DNA containing phage. Its morphogenetic pathway requires the formation of a precursor procapsid that subsequently matures to the capsid. The stability of bacteriophage P22 coat protein in both monomeric and polymeric forms under hydrostatic pressure has been examined previously [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00004a003
更新日期：1995-01-31 00:00:00
abstract：:Indolotryptoline natural products represent a small family of structurally unique chromopyrrolic acid-derived antiproliferative agents. Like many prospective anticancer agents before them, the exploration of their potential clinical utility has been hindered by the limited information known about their mechanism of ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501078j
更新日期：2014-11-18 00:00:00
abstract：:A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00189a008
更新日期：1994-06-14 00:00:00
abstract：:We reported previously that cdc2 kinase decreased the microtubule-stabilizing ability of a major HeLa cell microtubule-associated protein, MAP4, by phosphorylation in vitro [Ookata, K., et al. (1995) J. Cell Biol. 128, 849-862]. An important question raised by this study is whether MAP4 is indeed phosphorylated by cdc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971251w
更新日期：1997-12-16 00:00:00
abstract：:Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900785x
更新日期：2009-08-18 00:00:00
abstract：:The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00411a004
更新日期：1988-05-31 00:00:00
abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a041
更新日期：1985-04-09 00:00:00
abstract：:The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00623a015
更新日期：1977-02-22 00:00:00
abstract：:Studies are presented of the equilibrium binding of [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable binding of [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM and Kd2 = 7.7 +/- 4.6 microM, with equal numbers of binding sites. Both components are com...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00591a032
更新日期：1979-11-27 00:00:00
abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300456f
更新日期：2012-09-25 00:00:00
abstract：:As part of a larger effort to engineer the stability and hemin-binding properties of microsomal (Mc) cytochromes b(5) into rat liver outer mitochondrial membrane (OM) cytochrome (cyt) b(5), several mutants of rat OM cyt b(5) were prepared to study the effect of gradual and complete elimination of two extended hydropho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026005l
更新日期：2002-10-01 00:00:00
abstract：:The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00092a003
更新日期：1993-10-19 00:00:00
abstract：:Comparative primary structural analyses have begun to elucidate polymorphic residues and segments of the class I antigens of the major histocompatibility complex, at least some of which presumably contribute to determinants important in immune recognition events. HLA-A2 structural variants have been described which ar...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00267a042
更新日期：1982-11-23 00:00:00
abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9528006
更新日期：1996-06-11 00:00:00
abstract：:Mycobacterium tuberculosis and many other members of the Actinomycetes family produce mycothiol, i.e., 1-d-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-d-glucopyranoside (MSH or AcCys-GlcN-Ins), to act against oxidative and antibiotic stress. The biosynthesis of MSH is essential for cell growth and has bee...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7011492
更新日期：2007-10-09 00:00:00
