Primary quinone (QA) binding site of bacterial photosynthetic reaction centers: mutations at residue M265 probed by FTIR spectroscopy.

abstract：:Adenosine cyclic 3',5'-phosphate receptor protein (CRP or CAP) is a regulatory protein involved in the transcription of several operons in Escherichia coli. cAMP-independent, nonspecific complexes of CRP and DNA were investigated by photochemical cross-linking of the protein to nonspecific DNA, whose thymines are subs...

journal_title：Biochemistry

authors： Katouzian-Safadi M,Blazy B,Cremet JY,Le Caer JP,Rossier J,Charlier M

更新日期：1993-02-23 00:00:00

abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...

journal_title：Biochemistry

authors： Fioravanti E,Durá MA,Lascoux D,Micossi E,Franzetti B,McSweeney S

更新日期：2008-11-04 00:00:00

abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...

journal_title：Biochemistry

authors： Shaw GS,Sykes BD

更新日期：1996-06-11 00:00:00

abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...

journal_title：Biochemistry

authors： Tiepold M,Kliche W,Pfannstiel J,Faulstich H

更新日期：2000-02-15 00:00:00

abstract：:The selectivity of binding of Escherichia coli RNA polymerase holoenzyme to a promoter-containing fragment of T7 DNA has been investigated over a range of solution conditions by using a double-label nitrocellulose filter binding assay. A 32P-labeled HaeIII restriction fragment of T7 D111 DNA containing the A1 and D pr...

journal_title：Biochemistry

authors： Strauss HS,Burgess RR,Record MT Jr

更新日期：1980-07-22 00:00:00

abstract：:In polyglutamine (polyQ) containing fragments of the Huntington's disease protein huntingtin (htt), the N-terminal 17 amino acid htt(NT) segment serves as the core of α-helical oligomers whose reversible assembly locally concentrates the polyQ segments, thereby facilitating polyQ amyloid nucleation. A variety of aggre...

journal_title：Biochemistry

authors： Jayaraman M,Mishra R,Kodali R,Thakur AK,Koharudin LM,Gronenborn AM,Wetzel R

更新日期：2012-04-03 00:00:00

abstract：:F0F1 ATP synthase harnesses a transmembrane electrochemical gradient for the production of ATP. When operated in reverse, this multiprotein complex catalyzes ATP hydrolysis. In bacteria, the ε subunit is involved in regulating this ATPase activity. Also, ε is essential for coupling ATP hydrolysis (or synthesis) to pro...

journal_title：Biochemistry

authors： Rodriguez AD,Dunn SD,Konermann L

更新日期：2014-06-24 00:00:00

abstract：:In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...

journal_title：Biochemistry

authors： Zhang P,Martin PD,Purcarea C,Vaishnav A,Brunzelle JS,Fernando R,Guy-Evans HI,Evans DR,Edwards BF

更新日期：2009-02-03 00:00:00

abstract：:A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...

journal_title：Biochemistry

authors： Chen F,Ritter JK,Wang MG,McBride OW,Lubet RA,Owens IS

更新日期：1993-10-12 00:00:00

abstract：:HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...

journal_title：Biochemistry

authors： Velten M,Villoutreix BO,Ladjimi MM

更新日期：2000-01-18 00:00:00

abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

journal_title：Biochemistry

authors： Chen JY,Waterman MR

更新日期：1992-03-03 00:00:00

abstract：:The effects of detergent [deoxycholate (DOC) and phospholipid [dimyristoylphosphatidylcholine (DMPC)] environments on the rotational dynamics of the single tryptophan residue 26 of bacteriophage M13 coat protein have been investigated by using time-resolved single photon counting measurements of the fluorescence inten...

journal_title：Biochemistry

authors： Johnson ID,Hudson BS

更新日期：1989-07-25 00:00:00

abstract：:Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the structural features that would improve the activity of a thermophilic enzyme at less than optimal t...

journal_title：Biochemistry

authors： Hayashi S,Akanuma S,Onuki W,Tokunaga C,Yamagishi A

更新日期：2011-10-11 00:00:00

abstract：:Recent models for water oxidation in photosystem II postulate that the tyrosine Y(Z) radical, Y(Z)(*), abstracts both an electron and a proton from the Mn cluster during one or more steps in the catalytic cycle. This coupling of proton- and electron-transfer events is postulated to provide the necessary driving force ...

journal_title：Biochemistry

authors： Debus RJ,Campbell KA,Pham DP,Hays AM,Britt RD

更新日期：2000-05-30 00:00:00

abstract：:Dihydrofolate reductase (DHFR) is often used as a model system to study the relation between protein dynamics and catalysis. We have studied a number of variants of the cold-adapted DHFR from Moritella profunda (MpDHFR), in which the catalytically important M20 and FG loops have been altered, and present a comparison ...

journal_title：Biochemistry

authors： Behiry EM,Evans RM,Guo J,Loveridge EJ,Allemann RK

更新日期：2014-07-29 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:Chorismate mutase catalyzes the first committed step toward the biosynthesis of the aromatic amino acids, phenylalanine and tyrosine. While this biosynthetic pathway exists exclusively in the cell cytoplasm, the Mycobacterium tuberculosis enzyme has been shown to be secreted into the extracellular medium. The secretor...

journal_title：Biochemistry

authors： Qamra R,Prakash P,Aruna B,Hasnain SE,Mande SC

更新日期：2006-06-13 00:00:00

abstract：:The Pf1 gene 5 protein forms a large helical nucleoprotein complex (Mr = 3.1 x 10(7)) with single-stranded viral DNA, from which a 32 amino acid sequence rich in alanine, proline, and glutamine residues can be removed from the C-terminus by limited proteolysis. Sharp resonances in the 1H NMR spectrum of the Pf1 nucleo...

journal_title：Biochemistry

authors： Davis KG,Plyte SE,Robertson SR,Cooper A,Kneale GG

更新日期：1995-01-10 00:00:00

abstract：:Much interest is currently focused on understanding the detailed contribution that particular amino acid residues make in protein structure and function. Although the use of site-directed mutagenesis has greatly contributed to this goal, the approach is limited to the standard repertoire of twenty amino acids. Fluorin...

journal_title：Biochemistry

authors： Duewel H,Daub E,Robinson V,Honek JF

更新日期：1997-03-18 00:00:00

abstract：:Cytochrome c-553 from Desulfovibrio vulgaris exhibits a highly exposed heme and an unusually low reduction potential with respect to other c-type cytochromes. Solvent heme exposure has been indicated as one of the most important factors in modulating the midpoint potential of the redox center. To test this hypothesis,...

journal_title：Biochemistry

authors： Fantuzzi A,Sadeghi S,Valetti F,Rossi GL,Gilardi G

更新日期：2002-07-09 00:00:00

abstract：:Complex I of respiratory chains transfers electrons from NADH to ubiquinone, coupled to the translocation of protons across the membrane. Two alternative coupling mechanisms are being discussed, redox-driven or conformation-driven. Using "zero-length" cross-linking reagent and isolated hydrophilic domains of complex I...

journal_title：Biochemistry

authors： Berrisford JM,Thompson CJ,Sazanov LA

更新日期：2008-09-30 00:00:00

abstract：:This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...

journal_title：Biochemistry

authors： Murkin AS,Chou WK,Wakarchuk WW,Tanner ME

更新日期：2004-11-09 00:00:00

abstract：:During the past decade, there has been much debate about the enormous catalytic rate enhancement observed in enzymatic reactions involving carbanion intermediates. Our recent theoretical study has demonstrated the importance of the short strong hydrogen bond (SSHB) in the enzymatic reactions. Nevertheless, other recen...

journal_title：Biochemistry

authors： Kim KS,Kim D,Lee JY,Tarakeshwar P,Oh KS

更新日期：2002-04-23 00:00:00

abstract：:Heme oxygenase-1 (HO-1) is an enzyme that catalyzes the oxidative degradation of heme. Since free heme is toxic to cells, rapid degradation of heme is important for maintaining cellular health. There have been useful mechanistic studies of the HO reaction based on crystal structures; however, how HO-1 recognizes heme ...

journal_title：Biochemistry

authors： Harada E,Sugishima M,Harada J,Fukuyama K,Sugase K

更新日期：2015-01-20 00:00:00

abstract：:We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...

journal_title：Biochemistry

authors： Rodriguez H,Valentine MR,Holmquist GP,Akman SA,Termini J

更新日期：1999-12-14 00:00:00

abstract：:The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...

journal_title：Biochemistry

authors： Berdis AJ,Benkovic SJ

更新日期：1997-03-11 00:00:00

abstract：:A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...

journal_title：Biochemistry

authors： Levy MA,Brandt M,Greway AT

更新日期：1990-03-20 00:00:00

abstract：:The classical complement pathway, which plays a vital role in preventing infection, is initiated by the action of the serine proteases C1r and C1s. We have examined the hydrolysis of substrates representing cleavage sequences in the physiological substrates for C1s, C2 and C4. These studies showed that the P(1)'-P(4)'...

journal_title：Biochemistry

authors： O'Brien G,Quinsey NS,Whisstock JC,Pike RN

更新日期：2003-12-23 00:00:00

abstract：:We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of to...

journal_title：Biochemistry

authors： Li J,Burgess BK,Corbin JL

更新日期：1982-08-31 00:00:00

abstract：:Transient phosphorylation at an aspartate residue on the Spo0F protein is a central step in the phosphorelay signal transduction pathway controlling sporulation in Bacilli. The response regulator Spo0F-P is stable to hydrolysis (t1/2 > 24 h at 23 degrees C in the absence of Mg2+), allowing the use of nondenaturing PAG...

journal_title：Biochemistry

authors： Zapf JW,Hoch JA,Whiteley JM

更新日期：1996-03-05 00:00:00