Environmental modulation of M13 coat protein tryptophan fluorescence dynamics.

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum was purified and depleted of proteolipids by solubilization in Triton X-100 and by fractionation on a DE-52 column. The protein reconstituted by deoxycholate-cholate dialysis at low lipid to protein ratios (2-5 mg of lipid/mg of protein), with either dioleoylphosphatidylethano...

journal_title：Biochemistry

authors： Navarro J,Toivio-Kinnucan M,Racker E

更新日期：1984-01-03 00:00:00

abstract：:Previously [Luz et al. (1994) Biochemistry 33, 7239-7249], we determined that Cl(-)- and -HCO3-dependent pHi homeostasis was perturbed in multidrug resistant (MDR) cells created by transfecting LR73 Chinese hamster ovary fibroblasts with wild-type mu (murine) MDR 1 (Gros et al., 1991). Via single-cell photometry exper...

journal_title：Biochemistry

authors： Roepe PD,Weisburg JH,Luz JG,Hoffman MM,Wei LY

更新日期：1994-09-13 00:00:00

abstract：:According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...

journal_title：Biochemistry

authors： Kim HW,Shen TJ,Ho NT,Zou M,Tam MF,Ho C

更新日期：1996-05-28 00:00:00

abstract：:Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than ...

journal_title：Biochemistry

authors： Frost JW,Bender JL,Kadonaga JT,Knowles JR

更新日期：1984-09-11 00:00:00

abstract：:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200...

journal_title：Biochemistry

authors： Engel J

更新日期：1992-11-10 00:00:00

abstract：:Cytochrome oxidase proteoliposomes were prepared from bovine heart oxidase. Size distributions determined by quasi-elastic light scattering (QELS) showed that there was a small population of large vesicles (120-200-nm diameter) and a large population of small vesicles (50-100-nm diameter). Trapping cytochrome c inside...

journal_title：Biochemistry

authors： Cooper CE,Nicholls P

更新日期：1990-04-24 00:00:00

abstract：:The effects of cysteine modification and variations in pH on the equilibrium parameters for inducer and operator binding to the lactose repressor protein were examined. Operator binding affinity was minimally affected by increasing the pH from 7.5 to 9.2, whereas inducer binding was decreased for both the unliganded p...

journal_title：Biochemistry

authors： Daly TJ,Matthews KS

更新日期：1986-09-23 00:00:00

abstract：:Glycine oxidase gene from a strain of Bacillus subtilis was cloned and expressed in Escherichia coli. The purified enzyme was found, by mass spectrometry, to have a protein M(r) of 40763 (value of 40761.6 predicted from DNA sequence) and a FAD prosthetic group M(r) of 785.1 (theoretical value of 785.5). Glycine oxidas...

journal_title：Biochemistry

authors： Jamil F,Gardner QA,Bashir Q,Rashid N,Akhtar M

更新日期：2010-08-31 00:00:00

abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...

journal_title：Biochemistry

authors： Linske-O'Connell LI,Sherman F,McLendon G

更新日期：1995-05-30 00:00:00

abstract：:The 1.9 A X-ray crystal structure of human myeloperoxidase complexed with cyanide (R = 0.175, R(free) = 0.215) indicates that cyanide binds to the heme iron with a bent Fe-C-N angle of approximately 157 degrees, and binding is accompanied by movement of the iron atom by 0.2 A into the porphyrin plane. The bent orienta...

journal_title：Biochemistry

authors： Blair-Johnson M,Fiedler T,Fenna R

更新日期：2001-11-20 00:00:00

abstract：:The membrane protein Rv0899 (OmpATb) from Mycobacterium tuberculosis, has been proposed to act as an outer membrane porin and to contribute to the bacterium's adaptation to the acidic environment of the phagosome during infection. The gene is restricted to pathogenic mycobacteria and, thus, is an attractive candidate ...

journal_title：Biochemistry

authors： Teriete P,Yao Y,Kolodzik A,Yu J,Song H,Niederweis M,Marassi FM

更新日期：2010-04-06 00:00:00

abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

journal_title：Biochemistry

authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

更新日期：2012-01-17 00:00:00

abstract：:Human fibrinogen gamma-module comprising residues gamma148-411 was expressed in Escherichia coli and refolded in vitro. Differential scanning calorimetry revealed that in addition to the two previously identified independently folded thermolabile domains, one in each half of the module, the gamma-module also contains ...

journal_title：Biochemistry

authors： Medved L,Litvinovich S,Ugarova T,Matsuka Y,Ingham K

更新日期：1997-04-15 00:00:00

abstract：:The electric organ of the ray, Torpedo ocellata, can serve as a source for both the acetylcholine (ACh) receptor and its ionic channel. The two entities were identified by their specific binding of [3H]ACh and [3H]perhydrohistrionicotoxin ([3H]H12-HTX), respectively. Binding of [3H]H12-HTX was inhibited by certain dru...

journal_title：Biochemistry

authors： Eldefrawi ME,Eldefrawi AT,Mansour NA,Daly JW,Witkop B,Albuquerque EX

更新日期：1978-12-12 00:00:00

abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

journal_title：Biochemistry

authors： Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

更新日期：1997-11-18 00:00:00

abstract：:We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...

journal_title：Biochemistry

authors： Jennings ML,Nicknish JS

更新日期：1984-12-18 00:00:00

abstract：:The solution conformation of the ribonuclease barnase has been determined by using 1H nuclear magnetic resonance (NMR) spectroscopy. The 20 structures were calculated by using 853 interproton distance restraints obtained from analyses of two-dimensional nuclear Overhauser spectra, 72 phi and 53 chi 1 torsion angle res...

journal_title：Biochemistry

authors： Bycroft M,Ludvigsen S,Fersht AR,Poulsen FM

更新日期：1991-09-03 00:00:00

abstract：:Disruption of the cell membrane by the β-amyloid (Aβ) peptides has been considered as a main mechanism of Alzheimer's disease. The peptide-to-lipid molar ratio (P:L) varies over a broad range biologically. We report here that two of the previously observed Aβ evolution pathways, fibrillation and induction of vesicle f...

journal_title：Biochemistry

authors： Akinlolu RD,Nam M,Qiang W

更新日期：2015-06-09 00:00:00

abstract：:The kinetics of the slow folding and unfolding reactions of barstar, a bacterial ribonuclease inhibitor protein, have been studied at 23(+/-1) degrees C, pH 8, by the use of tryptophan fluorescence, far-UV circular dichroism (CD), near-UV CD, and transient mixing (1)H nuclear magnetic resonance (NMR) spectroscopic mea...

journal_title：Biochemistry

authors： Bhuyan AK,Udgaonkar JB

更新日期：1999-07-13 00:00:00

abstract：:The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR st...

journal_title：Biochemistry

authors： London RE,Gabel SA

更新日期：1988-10-04 00:00:00

abstract：:Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutio...

journal_title：Biochemistry

authors： Reece KL,Moss RL

更新日期：2008-05-06 00:00:00

abstract：:Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...

journal_title：Biochemistry

authors： Miyazaki M,Nakano M,Fukuda M,Handa T

更新日期：2009-08-18 00:00:00

abstract：:By use of six highly purified exoglycosidases with well-defined specificity, the oligosaccharide units of human plasma beta 2-glycoprotein I (beta 2I) were modified by sequential enzymatic degradation. The released monosaccharides (NeuAc, Gal, GlcNAc, and Man) were quantified, and the carbohydrate compositions of the ...

journal_title：Biochemistry

authors： Walsh MT,Watzlawick H,Putnam FW,Schmid K,Brossmer R

更新日期：1990-07-03 00:00:00

abstract：:Photosynthetic water oxidation takes place at the Mn4CaO5 cluster in photosystem II. Around the Mn4CaO5 cluster, a hydrogen bond network is formed by several water molecules, including four water ligands. To clarify the role of this water network in the mechanism of water oxidation, we investigated the effects of the ...

journal_title：Biochemistry

authors： Nakamura S,Ota K,Shibuya Y,Noguchi T

更新日期：2016-01-26 00:00:00

abstract：:Tripeptidyl peptidase II is a high molecular weight serine exopeptidase, which has been purified from rat liver and human erythrocytes. Four clones, representing 4453 bp, or 90% of the mRNA of the human enzyme, have been isolated from two different cDNA libraries. One clone, designated A2, was obtained after screening...

journal_title：Biochemistry

authors： Tomkinson B,Jonsson AK

更新日期：1991-01-08 00:00:00

abstract：:Alpha-synuclein (alpha-syn) is the major component of intracellular inclusions in several neurodegenerative diseases, and the conversion of soluble alpha-syn into filamentous aggregates may contribute to disease pathogenesis. Since mechanisms leading to the formation of alpha-syn inclusions are unclear, in vitro model...

journal_title：Biochemistry

authors： Murray IV,Giasson BI,Quinn SM,Koppaka V,Axelsen PH,Ischiropoulos H,Trojanowski JQ,Lee VM

更新日期：2003-07-22 00:00:00

abstract：:Multimodular enzymes, including polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and mixed PKS/NRPS systems, contain functional domains with similar functions. Domain swapping and module fusion are potential powerful strategies for creating hybrid enzymes to synthesize modified natural products. ...

journal_title：Biochemistry

authors： Suo Z

更新日期：2005-03-29 00:00:00

abstract：:A topoisomer gel retardation assay has been used to examine the topological requirements for the formation of open promoter complexes on DNA minicircles carrying sigma 54-dependent promoters. In the absence of intercalators, individual topoisomers carrying both the nifL and nifF promoters could be resolved as discrete...

journal_title：Biochemistry

authors： Qureshi M,Eydmann T,Austin S,Dixon R

更新日期：1997-10-07 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:Spectrophotometric titration of pyridoxamine-pyruvate transaminase (EC 2.6.1.30) with pyridoxal at pH 7.15 gives four equivalent binding sites per tetramer. The pH dependence of the equilibrium constant for the association of 5'-deoxypyridoxal with the active site lysine residue was determined spectrophotometrically. ...

journal_title：Biochemistry

authors： Gilmer PJ,McIntire WS,Kirsch JF

更新日期：1977-11-29 00:00:00