当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Mycobacterium tuberculosis Rv0899 adopts a mixed alpha/beta-structure and does not form a transmembrane beta-barrel.

Mycobacterium tuberculosis Rv0899 adopts a mixed alpha/beta-structure and does not form a transmembrane beta-barrel.

Abstract：

:The membrane protein Rv0899 (OmpATb) from Mycobacterium tuberculosis, has been proposed to act as an outer membrane porin and to contribute to the bacterium's adaptation to the acidic environment of the phagosome during infection. The gene is restricted to pathogenic mycobacteria and, thus, is an attractive candidate for the development of anti-tuberculosis chemotherapy. The 326-residue protein contains three domains: an N-terminal domain (residues 1-72) that includes a sequence of 20 hydrophobic amino acids required for membrane translocation, a central B domain (residues 73-200) with homology to the conserved putative lipid-binding BON (bacterial OsmY and nodulation) superfamily, and a C domain (residues 201-326) with homology to the OmpA-C-like superfamily of periplasmic peptidoglycan-binding sequences, found in several types of bacterial membrane proteins, including in the C-terminus of the Escherichia coli outer membrane protein OmpA. We have characterized the structure and dynamics of the B and C domains and have determined the three-dimensional structure of the B domain. Rv0899 does not form a transmembrane beta-barrel. Residues 73-326 form a mixed alpha/beta-globular structure, encompassing two independently folded modules corresponding to the B and C domains connected by a flexible linker. The B domain folds with three parallel/antiparallel alpha-helices packed against six parallel/antiparallel beta-strands that form a flat beta-sheet. The core is hydrophobic, while the exterior is polar and predominantly acidic. The structure of a BON homology domain is revealed here for the first time. In light of this unexpected structure, it is hard to reconcile an outer membrane porin activity with the central domain of the protein. The structure of the B domain and the overall architecture of the protein suggest alternative modes of membrane association.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Teriete P,Yao Y,Kolodzik A,Yu J,Song H,Niederweis M,Marassi FM

doi

10.1021/bi100158s

subject

Has Abstract

pub_date

2010-04-06 00:00:00

pages

2768-77

issue

13

eissn

0006-2960

issn

1520-4995

journal_volume

49

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structural and Mutagenesis Studies Evince the Role of the Extended Protuberant Domain of Ribosomal Protein uL10 in Protein Translation.

    abstract：:The lateral stalk of ribosomes constitutes the GTPase-associated center and is responsible for recruiting translation factors to the ribosomes. The eukaryotic stalk contains a P-complex, in which one molecule of uL10 (formerly known as P0) protein binds two copies of P1/P2 heterodimers. Unlike bacterial uL10, eukaryot...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00528

    authors： Choi KA,Yang L,Lee KM,Yu CW,Banfield DK,Ito K,Uchiumi T,Wong KB

    更新日期：2019-09-10 00:00:00

  • Comparative Analysis of CPI-Motif Regulation of Biochemical Functions of Actin Capping Protein.

    abstract：:The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00092

    authors： McConnell P,Mekel M,Kozlov AG,Mooren OL,Lohman TM,Cooper JA

    更新日期：2020-03-24 00:00:00

  • A high-affinity fluorenone-based beta 2-adrenergic receptor antagonist with a photoactivatable pharmacophore.

    abstract：:To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001342k

    authors： Wu Z,Ruoho AE

    更新日期：2000-10-24 00:00:00

  • γ-Synuclein: seeding of α-synuclein aggregation and transmission between cells.

    abstract：:Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseases. The synuclein family comprises three small naturally unfolded proteins implicated in neurodegenerative diseases and some forms of cancer. α-Synuclein is a soluble protein that forms toxic inclusions associated with ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300478w

    authors： Surgucheva I,Sharov VS,Surguchov A

    更新日期：2012-06-12 00:00:00

  • Chaotropic agents and increased matrix volume enhance binding of mitochondrial cyclophilin to the inner mitochondrial membrane and sensitize the mitochondrial permeability transition to [Ca2+].

    abstract：:Binding of mitochondrial cyclophilin (CyP) to the inner mitochondrial membrane is induced by treatment of mitochondria with thiol reagents or oxidative stress and correlates with a sensitization to [Ca2+] of the cyclosporin A-sensitive mitochondrial permeability transition pore (MTP) [Connern, C. P., & Halestrap, A. P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9525177

    authors： Connern CP,Halestrap AP

    更新日期：1996-06-25 00:00:00

  • Role of the conserved quartets of residues located in the N- and C-terminal halves of the transposon Tn10-encoded metal-tetracycline/H+ antiporter of Escherichia coli.

    abstract：:In the putative secondary structure of the transposon Tn10-encoded metal-tetracycline/H+ antiporter [Yamaguchi, et al. (1992) J. Biol. Chem. 267, 7490-7498], Tyr50-XXX-Gln54 in transmembrane helix 2 and Gly80-XXX-Asp84 in helix 3 are thought to face each other in the N-terminal region. At the corresponding positions i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00072a027

    authors： Yamaguchi A,Akasaka T,Kimura T,Sakai T,Adachi Y,Sawai T

    更新日期：1993-06-01 00:00:00

  • Isotope trapping and kinetic isotope effect studies of rat liver alpha-(2-->6)-sialyltransferase.

    abstract：:A mechanistic study of rat liver alpha-(2-->6) sialyltransferase (ST) is presented that includes isotope trapping experiments and kinetic isotope effects on V/K for the ST-catalyzed reaction of isotopically labeled CMP-N-acetylneuraminate and N-acetyllactosamine. The isotope trapping experiments confirmed that the kin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971624t

    authors： Bruner M,Horenstein BA

    更新日期：1998-01-06 00:00:00

  • Cofactor Complexes of DesD, a Model Enzyme in the Virulence-related NIS Synthetase Family.

    abstract：:The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00899

    authors： Hoffmann KM,Goncuian ES,Karimi KL,Amendola CR,Mojab Y,Wood KM,Prussia GA,Nix J,Yamamoto M,Lathan K,Orion IW

    更新日期：2020-09-22 00:00:00

  • Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis.

    abstract：:NanGIR1 is a catalytic element inserted in the P6 loop of a group I intron (NanGIR2) in the small subunit rRNA precursor of the protist Naegleria andersoni [Einvik, C., Decatur, W. A., Embley, T. M., Vogt, V. M., and Johansen, S. (1997) RNA 3, 710-720]. It catalyzes site-specific hydrolysis at an internal processing s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9718595

    authors： Jabri E,Aigner S,Cech TR

    更新日期：1997-12-23 00:00:00

  • Effect of the carbohydrate moiety on the secondary structure of beta 2-glycoprotein. I. Implications for the biosynthesis and folding of glycoproteins.

    abstract：:By use of six highly purified exoglycosidases with well-defined specificity, the oligosaccharide units of human plasma beta 2-glycoprotein I (beta 2I) were modified by sequential enzymatic degradation. The released monosaccharides (NeuAc, Gal, GlcNAc, and Man) were quantified, and the carbohydrate compositions of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00478a020

    authors： Walsh MT,Watzlawick H,Putnam FW,Schmid K,Brossmer R

    更新日期：1990-07-03 00:00:00

  • Characterization of recombinant Saccharomyces cerevisiae manganese-containing superoxide dismutase and its H30A and K170R mutants expressed in Escherichia coli.

    abstract：:All known Mn-containing superoxide dismutases (MnSODs) have a highly conserved histidine (His-30 in Escherichia coli FeSOD) in the active-site channel, and nearly all have an active-site arginine (Arg-170) that has been proposed to play a combined structural and functional role [Chan et al., Arch. Biochem. Biophys. 27...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980167t

    authors： Borders CL Jr,Bjerrum MJ,Schirmer MA,Oliver SG

    更新日期：1998-08-11 00:00:00

  • Enhanced processivity of nuclear matrix bound DNA polymerase alpha from regenerating rat liver.

    abstract：:Translocation of DNA during in vitro DNA synthesis on nuclear matrix bound replicational assemblies from regenerating rat liver was determined by measuring the processivity (average number of nucleotides added following one productive binding event of the polymerase to the DNA template) of nuclear matrix bound DNA pol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00392a020

    authors： Tubo RA,Martelli AM,Berezney R

    更新日期：1987-09-08 00:00:00

  • A transforming growth factor-alpha-Pseudomonas exotoxin hybrid protein undergoes pH-dependent conformational changes conducive to membrane interaction.

    abstract：:TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00064a037

    authors： Sanyal G,Marquis-Omer D,Gress JO,Middaugh CR

    更新日期：1993-04-06 00:00:00

  • Nearest neighbor analysis of the SecYEG complex. 2. Identification of a SecY-SecE cytosolic interface.

    abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034333v

    authors： Satoh Y,Mori H,Ito K

    更新日期：2003-06-24 00:00:00

  • Evidence for a recombination-independent pathway for the repair of DNA interstrand cross-links based on a site-specific study with nitrogen mustard.

    abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962778w

    authors： Berardini M,Mackay W,Loechler EL

    更新日期：1997-03-25 00:00:00

  • In vitro interaction between ceruloplasmin and human serum transferrin.

    abstract：:The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1014503

    authors： Ha-Duong NT,Eid C,Hémadi M,El Hage Chahine JM

    更新日期：2010-12-07 00:00:00

  • The amino- and carboxyl-terminal fragments of the Bacillus thuringensis Cyt1Aa toxin have differential roles in toxin oligomerization and pore formation.

    abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101239r

    authors： Rodriguez-Almazan C,Ruiz de Escudero I,Cantón PE,Muñoz-Garay C,Pérez C,Gill SS,Soberón M,Bravo A

    更新日期：2011-01-25 00:00:00

  • Dismutase activity of ADP-L-glycero-D-manno-heptose 6-epimerase: evidence for a direct oxidation/reduction mechanism.

    abstract：:The first positive evidence for the utilization of a direct C-6' ' oxidation/reduction mechanism by ADP-l-glycero-d-manno-heptose 6-epimerase is reported here. The epimerase (HldD or AGME, formerly RfaD) operates in the biosynthetic pathway of l-glycero-d-manno-heptose, which is a conserved sugar in the core region of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050106c

    authors： Morrison JP,Read JA,Coleman WG Jr,Tanner ME

    更新日期：2005-04-19 00:00:00

  • Structure-function relationship of islet-activating protein, pertussis toxin: biological activities of hybrid toxins reconstituted from native and methylated subunits.

    abstract：:Islet-activating protein (IAP), pertussis toxin, is a hexameric protein composed of an A protomer and a B oligomer, the residual pentamer having such a subunit assembly that two different dimers, dimer 1 and dimer 2, are connected with each other by means of the smallest C subunit. Incubation of IAP with formaldehyde ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00354a025

    authors： Nogimori K,Tamura M,Yajima M,Hashimura N,Ishii S,Ui M

    更新日期：1986-03-25 00:00:00

  • Melibiose permease of Escherichia coli: structural organization of cosubstrate binding sites as deduced from tryptophan fluorescence analyses.

    abstract：:Binding of the coupling ion (Na+ or Li+) and sugars to the purified melibiose permease of Escherichia coli, reconstituted in proteoliposomes, produces selective and cooperative changes of the transporter tryptophan fluorescence. To assess the individual contribution of N- or C-terminal domains of the permease to these...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961372g

    authors： Mus-Veteau I,Leblanc G

    更新日期：1996-09-17 00:00:00

  • Mutability of an HNH nuclease imidazole general base and exchange of a deprotonation mechanism.

    abstract：:Several unique protein folds that catalyze the hydrolysis of phosphodiester bonds have arisen independently in nature, including the PD(D/E)XK superfamily (typified by type II restriction endonucleases and many recombination and repair enzymes) and the HNH superfamily (found in an equally wide array of enzymes, includ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700418d

    authors： Eastberg JH,Eklund J,Monnat R Jr,Stoddard BL

    更新日期：2007-06-19 00:00:00

  • One-electron reduction of D-amino acid oxidase. Kinetics of conversion from the red semiquinone to the blue semiquinone.

    abstract：:The reduction of D-amino acid oxidase (DAAO) by hydrated electrons (eaq-) has been studied in the absence and presence of benzoate by pulse radiolysis. The eaq-did not reduce the flavin moiety in DAAO and reacted with the amino acid residues in the protein. In the presence of benzoate, eaq- first reacted with benzoate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00278a028

    authors： Kobayashi K,Hirota K,Ohara H,Hayashi K,Miura R,Yamano T

    更新日期：1983-04-26 00:00:00

  • Posttranslational modifications of the Ia-associated invariant protein p41 after gene transfer.

    abstract：:Biochemical analysis of a rat fibroblast cell clone, transfected with the murine Ia associated invariant chain gene, demonstrates the expression of a family of proteins. This indicates that all members of the invariant protein family are derived from the same gene. The proteins p41, Ii, p27, p25, and p10, synthesized ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00411a028

    authors： Koch N

    更新日期：1988-05-31 00:00:00

  • Intramolecular disulfide loop formation in a peptide containing two cysteines.

    abstract：:The cyanogen bromide fragment comprising residues 115-181 of Kunitz soybean trypsin inhibitor is a soluble random-coil peptide at pH 7 containing two cysteines separated by eight other amino acids in the primary sequence. Four of the six rate constants have been determined for the three disulfide exchange reactions be...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00377a005

    authors： Snyder GH

    更新日期：1987-02-10 00:00:00

  • Studies of the cryptic allylic pyrophosphate isomerase activity of trichodiene synthase using the anomalous substrate 6,7-dihydrofarnesyl pyrophosphate.

    abstract：:Two enantiomeric analogues of farnesyl pyrophosphate (1) were tested as inhibitors and anomalous substrates of trichodiene synthase, which catalyzes the cyclization of trans,trans-farnesyl pyrophosphate (1) to the sesquiterpene hydrocarbon trichodiene (2). The reaction has been shown to involve preliminary isomerizati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00475a011

    authors： Cane DE,Pawlak JL,Horak RM

    更新日期：1990-06-12 00:00:00

  • Expression and sequence analyses of serum amyloid A in the Syrian hamster.

    abstract：:Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have stu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00437a040

    authors： Webb CF,Tucker PW,Dowton SB

    更新日期：1989-05-30 00:00:00

  • Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration.

    abstract：:Nitric oxide (NO) regulates cardiovascular hemostasis by binding to soluble guanylyl cyclase (sGC), leading to cGMP production, reduced cytosolic calcium concentration ([Ca(2+)](i)), and vasorelaxation. Thrombospondin-1 (TSP-1), a secreted matricellular protein, was recently discovered to inhibit NO signaling and sGC ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201060c

    authors： Ramanathan S,Mazzalupo S,Boitano S,Montfort WR

    更新日期：2011-09-13 00:00:00

  • Differential coupling of smooth and skeletal muscle pyruvate kinase to creatine kinase.

    abstract：:The interaction of pyruvate kinase from skeletal (SKPK) and smooth (SMPK) muscle with MM-creatine kinase (MMCK) and BB-creatine kinase (BBCK) was assessed using temporal absorbance changes, variations in absorbance at different wavelengths, concentration dependence, association in an electric field, and PK kinetic act...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991076e

    authors： Sears PR,Dillon PF

    更新日期：1999-11-09 00:00:00

  • Erythrocyte band 3 protein: evidence for multiple membrane-crossing segments in the 17 000-dalton chymotryptic fragment.

    abstract：:We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00321a024

    authors： Jennings ML,Nicknish JS

    更新日期：1984-12-18 00:00:00

  • Proton Transfer and Tautomerism in 2-Aminopurine-Thymine and Pyrrolocytosine-Guanine Base Pairs.

    abstract：:Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00521

    authors： Bull GD,Thompson KC

    更新日期：2018-07-31 00:00:00