Intramolecular disulfide loop formation in a peptide containing two cysteines.

abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...

journal_title：Biochemistry

authors： Haunerland NH,Jacobson BL,Wesenberg G,Rayment I,Holden HM

更新日期：1994-10-18 00:00:00

abstract：:Multidrug resistance protein (MRP) and P-glycoprotein (Pgp) are both members of the superfamily of ATP binding cassette plasma membrane drug transport proteins, which may be partly responsible for multidrug resistance of tumor cells. Although MRP has been identified as an organic anion transporter and Pgp as a transpo...

journal_title：Biochemistry

authors： Essodaigui M,Broxterman HJ,Garnier-Suillerot A

更新日期：1998-02-24 00:00:00

abstract：:The effects of poly(ethylene glycol) (PEG) on sonicated unilamellar vesicles made of phosphatidylcholine have been examined. Stopped-flow and equilibrium data are presented for vesicle aggregation, vesicle leakage, lipid mixing, and aqueous contents mixing. Vesicle aggregation is detected as a monoexponential increase...

journal_title：Biochemistry

authors： Viguera AR,Mencía M,Goñi FM

更新日期：1993-04-13 00:00:00

abstract：:Miniproteins reduce the complexity of the protein-folding problem allowing systematic studies of contributions to protein folding and stabilization. Here, we describe the rational redesign of a miniprotein, PPα, comprising a polyproline II helix, a loop, and an α helix. The redesign provides a de novo framework for in...

journal_title：Biochemistry

authors： Porter Goff KL,Nicol D,Williams C,Crump MP,Zieleniewski F,Samphire JL,Baker EG,Woolfson DN

更新日期：2019-07-16 00:00:00

abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...

journal_title：Biochemistry

authors： Makde RD,Mahajan SK,Kumar V

更新日期：2007-02-27 00:00:00

abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...

journal_title：Biochemistry

authors： Masuya T,Murai M,Morisaka H,Miyoshi H

更新日期：2014-12-16 00:00:00

abstract：:A new series of peptide inhibitors of human Factor VIIa (FVIIa) has been identified and affinity matured from naive and partially randomized peptide phage libraries selected against the immobilized tissue factor x Factor VIIa (TF x FVIIa) complex. These "A-series" peptides contain a single disulfide bond and a 13-resi...

journal_title：Biochemistry

authors： Dennis MS,Roberge M,Quan C,Lazarus RA

更新日期：2001-08-14 00:00:00

abstract：:Cytochrome P450BM3 is a bacterial enzyme with a heme cofactor that binds small diatomic ligands. Here we report the first study of carbon monoxide (CO) photodissociation and rebinding in ferrous P450BM3 on an ultrafast time scale. We monitored dissociation of carbon monoxide upon Soret band excitation using visible an...

journal_title：Biochemistry

authors： Rupenyan A,Commandeur J,Groot ML

更新日期：2009-07-07 00:00:00

abstract：:The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...

journal_title：Biochemistry

authors： Reiling KK,Endres NF,Dauber DS,Craik CS,Stroud RM

更新日期：2002-04-09 00:00:00

abstract：:The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanni...

journal_title：Biochemistry

authors： Perkins WJ,Wells JA,Yount RG

更新日期：1984-08-14 00:00:00

abstract：:A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...

journal_title：Biochemistry

authors： le Coutre J,Turk E,Kaback HR,Wright EM

更新日期：2002-06-25 00:00:00

abstract：:The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at differe...

journal_title：Biochemistry

authors： Thompson JF,Bachellerie JP,Hall K,Hearst JE

更新日期：1982-03-16 00:00:00

abstract：:Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

journal_title：Biochemistry

authors： Rudzki JE,Peters KS

更新日期：1984-08-14 00:00:00

abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

journal_title：Biochemistry

authors： Ribas de Pouplana L,Schimmel P

更新日期：1997-12-09 00:00:00

abstract：:Heme-derived linear tetrapyrroles (phytobilins) in phycobiliproteins and phytochromes perform critical light-harvesting and light-sensing roles in oxygenic photosynthetic organisms. A key enzyme in their biogenesis, phycocyanobilin:ferredoxin oxidoreductase (PcyA), catalyzes the overall four-electron reduction of bili...

journal_title：Biochemistry

authors： Kohler AC,Gae DD,Richley MA,Stoll S,Gunn A,Lim S,Martin SS,Doukov TI,Britt RD,Ames JB,Lagarias JC,Fisher AJ

更新日期：2010-07-27 00:00:00

abstract：:The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...

journal_title：Biochemistry

authors： Gao X,Brown FK,Jeffs P,Bischofberger N,Lin KY,Pipe AJ,Noble SA

更新日期：1992-07-14 00:00:00

abstract：:PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...

journal_title：Biochemistry

authors： RoseFigura JM,Puehringer S,Schwarzenbacher R,Toyama H,Klinman JP

更新日期：2011-03-08 00:00:00

abstract：:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system m...

journal_title：Biochemistry

authors： Silversmith RE,Nelsestuen GL

更新日期：1986-11-18 00:00:00

abstract：:Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...

journal_title：Biochemistry

authors： Ahmad TY,Beaudet AL,Sparrow JT,Morrisett JD

更新日期：1986-07-29 00:00:00

abstract：:Phospholipids and sterols play multiple roles in cells. In addition to establishing barriers between compartments, they also provide the matrix for assembly and function of a large variety of catalytic processes. Lipid composition is a highly regulated feature of biological membranes, yet its implications for membrane...

journal_title：Biochemistry

authors： Autzen HE,Koldsø H,Stansfeld PJ,Gourdon P,Sansom MSP,Nissen P

更新日期：2018-07-17 00:00:00

abstract：:The increase of the proton motive force mediated by bacteriorhodopsin is compared to the intracellular ATP concentration under steady-state illumination. The membrane potential was measured via the accumulation of the lipophilic ion [14C]triphenylmethylphosphonium and the pH gradient via the accumulation of the weak a...

journal_title：Biochemistry

authors： Michel H,Oesterhelt D

更新日期：1980-09-30 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:Reducing sugars such as glucose react nonenzymatically with protein amino groups to initiate a posttranslational modification process known as advanced glycosylation. Nucleotide bases also participate in advanced glycosylation reactions, producing DNA-linked advanced glycosylation endproducts (AGEs) that cause mutatio...

journal_title：Biochemistry

authors： Papoulis A,al-Abed Y,Bucala R

更新日期：1995-01-17 00:00:00

abstract：:Furanacryloyl-Phe-Gly-Gly has been shown to be a convenient substrate for angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1). A detailed kinetic analysis of the hydrolysis of this substrate indicates normal Michaelis-Menten behavior with kcat = 19000 min-1 and KM = 3.0 x 10(-4) M determined at pH...

journal_title：Biochemistry

authors： Bünning P,Holmquist B,Riordan JF

更新日期：1983-01-04 00:00:00

abstract：:Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequence...

journal_title：Biochemistry

authors： Kudlicki W,Chirgwin J,Kramer G,Hardesty B

更新日期：1995-11-07 00:00:00

abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...

journal_title：Biochemistry

authors： Fredenburg RA,Rospigliosi C,Meray RK,Kessler JC,Lashuel HA,Eliezer D,Lansbury PT Jr

更新日期：2007-06-19 00:00:00

abstract：:We describe the development and characterization of substance P labeled at Lys3 with fluorescein ([fluorescein Lys3]SP) as a fluorescent probe for the neurokinin 1 (NK1) receptor. [fluorescein Lys3]SP is an agonist at the human NK1 receptor, with an affinity for both the high-affinity and low-affinity binding states o...

journal_title：Biochemistry

authors： Tota MR,Daniel S,Sirotina A,Mazina KE,Fong TM,Longmore J,Strader CD

更新日期：1994-11-08 00:00:00

abstract：:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to ...

journal_title：Biochemistry

authors： Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HO

更新日期：2003-05-27 00:00:00

abstract：:Adeno-associated virus (AAV) nonstructural proteins Rep78 and Rep68 carry out all DNA transactions that regulate the AAV life cycle. They share two multifunctional domains: an N-terminal origin binding/nicking domain (OBD) from the HUH superfamily and a SF3 helicase domain. A short linker of ∼20 amino acids that is cr...

journal_title：Biochemistry

authors： Musayev FN,Zarate-Perez F,Bardelli M,Bishop C,Saniev EF,Linden RM,Henckaerts E,Escalante CR

更新日期：2015-09-29 00:00:00

abstract：:Escherichia coli dihydrofolate reductase contains five tryptophan residues that are spatially distributed throughout the protein and located in different secondary structural elements. When these tryptophans are replaced with [6-19F]tryptophan, distinct native and unfolded resonances can be resolved in the 1-D 19F NMR...

journal_title：Biochemistry

authors： Hoeltzli SD,Frieden C

更新日期：1998-01-06 00:00:00