Ligand-induced differences in secondary structure of the Vibrio parahaemolyticus Na+/galactose cotransporter.

abstract：:The 70-kDa subunit of eukaryotic replication protein A (RPA) contains a conserved four cysteine-type zinc-finger motif that has been implicated in regulation of DNA replication and repair. Unlike other zinc-finger proteins, RPA zinc-finger motif is not a DNA-binding component, and deletion of the zinc-finger had very ...

journal_title：Biochemistry

authors： You JS,Wang M,Lee SH

更新日期：2000-10-24 00:00:00

abstract：:Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...

journal_title：Biochemistry

authors： Rajala A,Daly RJ,Tanito M,Allen DT,Holt LJ,Lobanova ES,Arshavsky VY,Rajala RV

更新日期：2009-06-23 00:00:00

abstract：:Factors which influence the accessibility, or exposure, of the carbohydrate head group of the glycolipid galactosylceramide (GalCer) at the membrane surface have been examined in lipid model membranes using the technique of galactose oxidase-tritiated sodium borohydride labeling. Both the ceramide composition of GalCe...

journal_title：Biochemistry

authors： Stewart RJ,Boggs JM

更新日期：1993-06-01 00:00:00

abstract：:Dynamic conformations of two distinct immunoglobulin (Ig) isotypes, murine IgE and human IgG1, were examined with fluorescence resonance energy transfer measurements. The IgE mutant epsilon/C gamma 3* and the IgG1 mutant gamma/C gamma 3* each bind [5-(dimethylamino)naphthalen-1-yl]sulfonyl (DNS) in two identical antig...

journal_title：Biochemistry

authors： Zheng Y,Shopes B,Holowka D,Baird B

更新日期：1992-08-25 00:00:00

abstract：:We report the first experimental characterization of the full thermodynamic profile for binding of urea to a native protein. We measured the volumetric parameters of lysozyme at pH 7.0 as a function of urea within a temperature range of 18-45 °C. At neutral pH, lysozyme retains its native conformation between 0 and 8 ...

journal_title：Biochemistry

authors： Son I,Chalikian TV

更新日期：2016-11-29 00:00:00

abstract：:The inactivation of excited rhodopsin in the presence of ATP, rhodopsin kinase, and/or arrestin has been studied from its effect on the two subsequent steps in the light-induced enzymatic cascade: metarhodopsin II catalyzed activation of G-protein and G-protein-dependent activation of cGMP phosphodiesterase. The inact...

journal_title：Biochemistry

authors： Bennett N,Sitaramayya A

更新日期：1988-03-08 00:00:00

abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...

journal_title：Biochemistry

authors： Sánchez del Pino MM,Fersht AR

更新日期：1997-05-06 00:00:00

abstract：:Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods. FTIR and visible CD were used for assessing changes of the secondary structure and coarse alterations of the [3Fe4S] and [4Fe4S] cluster moieties, respectively. Fine details of the ...

journal_title：Biochemistry

authors： Todorovic S,Leal SS,Salgueiro CA,Zebger I,Hildebrandt P,Murgida DH,Gomes CM

更新日期：2007-09-18 00:00:00

abstract：:The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...

journal_title：Biochemistry

authors： Weber PL,Wemmer DE,Reid BR

更新日期：1985-08-13 00:00:00

abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...

journal_title：Biochemistry

authors： Boral S,Maiti S,Basak AJ,Lee W,De S

更新日期：2020-12-22 00:00:00

abstract：:Heme oxygenase-1 (HO-1) is an enzyme that catalyzes the oxidative degradation of heme. Since free heme is toxic to cells, rapid degradation of heme is important for maintaining cellular health. There have been useful mechanistic studies of the HO reaction based on crystal structures; however, how HO-1 recognizes heme ...

journal_title：Biochemistry

authors： Harada E,Sugishima M,Harada J,Fukuyama K,Sugase K

更新日期：2015-01-20 00:00:00

abstract：:The effect of araC incorporation into the dodecamer duplex [d(CGCGAATT) (araC)d(GCG)]2 was examined by comparing its nuclear magnetic resonance (NMR)-determined solution structure with that of the control duplex d[(CGCGAATTCGCG)]2. 1H and 31P resonances in both duplexes were assigned using a combination of 2-D 1H NMR ...

journal_title：Biochemistry

authors： Schweitzer BI,Mikita T,Kellogg GW,Gardner KH,Beardsley GP

更新日期：1994-09-27 00:00:00

abstract：:F(1)-ATPase mutations in Escherichia coli that changed the strength of hydrogen bonds between the alpha and beta subunits in a location that links the catalytic site to the interface between the beta catch loop and the gamma subunit were examined. Loss of the ability to form the hydrogen bonds involving alphaS337, bet...

journal_title：Biochemistry

authors： Boltz KW,Frasch WD

更新日期：2006-09-19 00:00:00

abstract：:Parkinson's disease (PD) involves loss of dopaminergic neurons in the substantia nigra and is characterized by intracellular inclusions, Lewy bodies, consisting primarily of aggregated alpha-synuclein. Two substitution mutations (A53T and A30P) in alpha-synuclein gene have been identified in familial early-onset PD. T...

journal_title：Biochemistry

authors： Moussa CE,Wersinger C,Tomita Y,Sidhu A

更新日期：2004-05-11 00:00:00

abstract：:A ribonuclease was isolated from serum-free supernatants of the human colon adenocarcinoma cell line HT-29. It was purified by cation-exchange and C18 reversed-phase high-performance liquid chromatography. The protein is basic, has a molecular weight of approximately 16,000, and has an amino acid composition that is s...

journal_title：Biochemistry

authors： Shapiro R,Fett JW,Strydom DJ,Vallee BL

更新日期：1986-11-18 00:00:00

abstract：:Glycinamide ribonucleotide transformylase, the first of the two formyl group transferases of de novo purine biosynthesis requiring 10-formyltetrahydrofolate, has been purified 1500-fold, nearly to homogeneity, from the murine lymphoma cell line L5178Y. Purification of the enzyme was facilitated by the use of a gelatin...

journal_title：Biochemistry

authors： Caperelli CA

更新日期：1985-03-12 00:00:00

abstract：:The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates with partial reduction of S-S bridges in the extracellular portion of the beta...

journal_title：Biochemistry

authors： Lutsenko S,Kaplan JH

更新日期：1993-07-06 00:00:00

abstract：:The kinetics of the slow folding and unfolding reactions of barstar, a bacterial ribonuclease inhibitor protein, have been studied at 23(+/-1) degrees C, pH 8, by the use of tryptophan fluorescence, far-UV circular dichroism (CD), near-UV CD, and transient mixing (1)H nuclear magnetic resonance (NMR) spectroscopic mea...

journal_title：Biochemistry

authors： Bhuyan AK,Udgaonkar JB

更新日期：1999-07-13 00:00:00

abstract：:In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferase...

journal_title：Biochemistry

authors： Yeh HW,Wu T,Chen M,Ai HW

更新日期：2019-03-26 00:00:00

abstract：:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...

journal_title：Biochemistry

authors： Chi LM,Lam SL

更新日期：2009-12-08 00:00:00

abstract：:DNA topoisomerase I (Top1) is an essential nuclear enzyme and a validated target for anticancer agent screening. In a previous study, we found that indolizinoquinoline-5,12-dione derivatives show significant biological activity against several human cancer cell lines. To understand their mechanism of inhibition of can...

journal_title：Biochemistry

authors： Wu N,Wu XW,Agama K,Pommier Y,Du J,Li D,Gu LQ,Huang ZS,An LK

更新日期：2010-11-30 00:00:00

abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...

journal_title：Biochemistry

authors： Jones DH,Barber KR,Grant CW

更新日期：1996-04-16 00:00:00

abstract：:A 15-residue fragment within the major continuous domain of gp120 from HIV-1 that can bind independently to the CD4 receptor has been shown to have the property of behaving as a solvent polarity-dependent conformational switch. The switch behavior (cooperative transition from beta-sheet to helical conformation as a fu...

journal_title：Biochemistry

authors： Graf von Stosch A,Kinzel V,Reed J

更新日期：1996-01-16 00:00:00

abstract：:ncd is a kinesin-related motor protein from Drosophila that moves in the opposite direction along microtubules to kinesin. To learn more about the ncd mechanism, ncd motor domain (R335-K700) was expressed in Escherichia coli and its enzymatic characteristics were studied. The ncd motor domain was purified from the cel...

journal_title：Biochemistry

authors： Shimizu T,Sablin E,Vale RD,Fletterick R,Pechatnikova E,Taylor EW

更新日期：1995-10-10 00:00:00

abstract：:Cytochrome bo from Escherichia coli is a ubiquinol oxidase which is a member of the superfamily of heme-copper respiratory oxidases. This superfamily, which includes the eukaryotic cytochrome c oxidases, has in common a bimetallic center consisting of a high-spin heme component and a copper atom (CuB) which is the sit...

journal_title：Biochemistry

authors： Thomas JW,Lemieux LJ,Alben JO,Gennis RB

更新日期：1993-10-19 00:00:00

abstract：:The interaction between the apoprotein of 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans and the prosthetic group FAD has been investigated by 13C, 15N, and 31P NMR techniques. The FAD prosthetic group was selectively enriched in 13C and 15N isotopes by adding isotopically labeled riboflavin derivatives to the...

journal_title：Biochemistry

authors： Pust S,Vervoort J,Decker K,Bacher A,Müller F

更新日期：1989-01-24 00:00:00

abstract：:The cytidine repressor (CytR) is a member of the LacR family of bacterial repressors with distinct functional features. The Escherichia coli CytR regulon comprises nine operons whose palindromic operators vary in both sequence and, most significantly, spacing between the recognition half-sites. This suggests a strong ...

journal_title：Biochemistry

authors： Moody CL,Tretyachenko-Ladokhina V,Laue TM,Senear DF,Cocco MJ

更新日期：2011-08-09 00:00:00

abstract：:Locusta migratoria apolipophorin III (apoLp-III) is a helix bundle exchangeable apolipoprotein that reversibly binds to lipoprotein surfaces. Structural reorganization of its five amphipathic alpha-helices enables the transition from the lipid-free to lipid-bound state. ApoLp-III-induced transformation of dimyristoylp...

journal_title：Biochemistry

authors： Weers PM,Kay CM,Ryan RO

更新日期：2001-06-26 00:00:00

abstract：:Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...

journal_title：Biochemistry

authors： Fedosov SN,Fedosova NU,Berglund L,Moestrup SK,Nexø E,Petersen TE

更新日期：2005-03-08 00:00:00

abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

journal_title：Biochemistry

authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

更新日期：1989-05-30 00:00:00