Abstract：
:The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Thompson JF,Bachellerie JP,Hall K,Hearst JEdoi
10.1021/bi00535a040subject
Has Abstractpub_date
1982-03-16 00:00:00pages
1363-8issue
6eissn
0006-2960issn
1520-4995journal_volume
21pub_type
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