Probing structural factors stabilizing antisense oligonucleotide duplexes: NMR studies of a DNA.DNA duplex containing a formacetal linkage.

abstract：:Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...

journal_title：Biochemistry

authors： Lennon BW,Williams CH Jr

更新日期：1996-04-16 00:00:00

abstract：:Ligand-dependent site-site (or subunit-subunit) interactions provide the basis for explaining cooperativity in chemical reactions. Even in the simplest possible nonaggregating system, interpretation of the interactions in terms of structural details requires an explicit assumption (or model) for the binding of the lig...

journal_title：Biochemistry

authors： Saroff HA

更新日期：1991-10-22 00:00:00

abstract：:The kinetics of charge recombination following photoexcitation by a laser pulse have been analyzed in the reaction center-light harvesting complex 1 (RC-LH1) purified from the photosynthetic bacterium Rhodobacter sphaeroides. In RC-LH1 core complexes isolated from photosynthetically grown cells P(+)Q(B)(-) recombines ...

journal_title：Biochemistry

authors： Francia F,Dezi M,Rebecchi A,Mallardi A,Palazzo G,Melandri BA,Venturoli G

更新日期：2004-11-09 00:00:00

abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...

journal_title：Biochemistry

authors： Chang CY,Stellwagen NC

更新日期：2011-10-25 00:00:00

abstract：:We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) correlating with major drug resistance, M46L, G48V, and V82S, and five o...

journal_title：Biochemistry

authors： Park JH,Sayer JM,Aniana A,Yu X,Weber IT,Harrison RW,Louis JM

更新日期：2016-04-26 00:00:00

abstract：:Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...

journal_title：Biochemistry

authors： Hammarström P,Sekijima Y,White JT,Wiseman RL,Lim A,Costello CE,Altland K,Garzuly F,Budka H,Kelly JW

更新日期：2003-06-10 00:00:00

abstract：:Kinetics of reduction of phototrophic bacterial flavocytochromes c by exogenous flavin semiquinones and fully reduced flavins generated by laser flash photolysis have been studied. The mechanisms of reduction of Chromatium and Chlorobium flavocytochromes c are more similar to one another than previously thought. Neith...

journal_title：Biochemistry

authors： Cusanovich MA,Meyer TE,Tollin G

更新日期：1985-03-12 00:00:00

abstract：:Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...

journal_title：Biochemistry

authors： Clarkson MW,Gilmore SA,Edgell MH,Lee AL

更新日期：2006-06-27 00:00:00

abstract：:The fluorescence properties of three variants of alpha-lactalbumin (alpha-LA) containing a single tryptophan residue were investigated under native, molten globule, and unfolded conditions. These proteins have levels of secondary structure and stability similar to those of the wild type. The fluorescence signal in the...

journal_title：Biochemistry

authors： Chakraborty S,Ittah V,Bai P,Luo L,Haas E,Peng Z

更新日期：2001-06-19 00:00:00

abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

journal_title：Biochemistry

authors： Ribas de Pouplana L,Schimmel P

更新日期：1997-12-09 00:00:00

abstract：:The membrane topology model of the Na(+)-citrate transporter CitS of Klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. A split version of CitS was constructed to study domain interactions and proximity relationships of...

journal_title：Biochemistry

authors： Dobrowolski A,Fusetti F,Lolkema JS

更新日期：2010-06-01 00:00:00

abstract：:Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...

journal_title：Biochemistry

authors： Babavali M,Esmann M,Fedosova NU,Marsh D

更新日期：2009-09-29 00:00:00

abstract：:Activation of washed human platelets initiated with alpha-thrombin, SFLLRN, or AYPGKF invariably results in the generation of PAR-1-(1-41) and PAR-4-(1-47). PAR-1-(1-41) and PAR-4-(1-47) are amino-terminal peptides generated when PAR-1 and -4 are cleaved in their first extracellular domains after R(41) and R(47), resp...

journal_title：Biochemistry

authors： Ofosu FA,Dewar L,Song Y,Cedrone AC,Hortelano G,Craven SJ

更新日期：2009-02-24 00:00:00

abstract：:One-carbon units are essential to a variety of anabolic processes which yield necessary cellular components including purines, pyrimidines, amino acids, and lipids. Serine hydroxymethyltransferase (SHMT) is the major provider of one-carbon units in the cell. The other product of this reaction is glycine. Both of these...

journal_title：Biochemistry

authors： Kastanos EK,Woldman YY,Appling DR

更新日期：1997-12-02 00:00:00

abstract：:Phe-tRNAPhe species modified on the 3'-terminal ribose residue were investigated for their ability to participate in individual steps of the elongation cycle using eukaryotic ribosomes from reticulocytes. None of the Phe-tRNAs used, namely Phe-tRNAPhe-C-C-3'dA, Phe-tRNAPhe-C-C-3'-NH2A, and Phe-tRNAPhe-C-C-Aoxi-red, ca...

journal_title：Biochemistry

authors： Baksht E,de Groot N,Sprinzl M,Cramer F

更新日期：1976-08-10 00:00:00

abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...

journal_title：Biochemistry

authors： Jackson RC

更新日期：1976-12-14 00:00:00

abstract：:Tk-subtilisin from the hyperthermophiolic archaeon Thermococcus kodakaraensis matures from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide [Tanaka, S., Saito, K., Chon, H., Matsumura, H., Koga, Y., Takano, K., and Kanaya, S. (2007) J. Biol. Chem. 282, 8246-8255]. It requires Ca(2+) for folding a...

journal_title：Biochemistry

authors： Takeuchi Y,Tanaka S,Matsumura H,Koga Y,Takano K,Kanaya S

更新日期：2009-11-10 00:00:00

abstract：:Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...

journal_title：Biochemistry

authors： Sudheendra US,Bechinger B

更新日期：2005-09-13 00:00:00

abstract：:Vibrational circular dichroism (VCD) and Fourier transform IR (FTIR) were measured for a series of short alanine-based peptides having the general formula Ac-(AAKAA)n-GY-NH2 (n = 1-4) from 5 to 50 degrees C in D2O and at room temperature in both TFE and H2O. In both of these latter solvents, the dominant structural fo...

journal_title：Biochemistry

authors： Yoder G,Pancoska P,Keiderling TA

更新日期：1997-12-09 00:00:00

abstract：:Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...

journal_title：Biochemistry

authors： Renthal R,Lanyi JK

更新日期：1976-05-18 00:00:00

abstract：:The heat production for the reaction steps of ATP hydrolysis by heavy meromyosin has been reexamined by using a reaction-type calorimeter. So far, the values reported for the heat production have varied substantially among research groups. The present results obtained in 0.3 M KCl, 10 mM MgCl2, and 20 mM Tris-HCl at p...

journal_title：Biochemistry

authors： Yamada T,Shimizu H,Suga H

更新日期：1981-07-21 00:00:00

abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...

journal_title：Biochemistry

authors： Wu HY,Behe MJ

更新日期：1985-09-24 00:00:00

abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...

journal_title：Biochemistry

authors： AbdulSalam SF,Thowfeik FS,Merino EJ

更新日期：2016-09-27 00:00:00

abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...

journal_title：Biochemistry

authors： Seifert T,Bartholmes P,Jaenicke R

更新日期：1985-01-15 00:00:00

abstract：:The intercellular environment is known to be very different from the environment where most of the elementary biological processes are studied in the laboratory. As a result, there was a considerable effort on cell mimicking either by confinement or by introducing macromolecular crowding. In the present study, dextran...

journal_title：Biochemistry

authors： Das N,Sen P

更新日期：2018-10-23 00:00:00

abstract：:Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...

journal_title：Biochemistry

authors： Kakuyama H,Söderberg L,Horigome K,Winblad B,Dahlqvist C,Näslund J,Tjernberg LO

更新日期：2005-11-29 00:00:00

abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...

journal_title：Biochemistry

authors： Zornetzer GA,Fox BG,Markley JL

更新日期：2006-04-25 00:00:00

abstract：:The interaction between the apoprotein of 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans and the prosthetic group FAD has been investigated by 13C, 15N, and 31P NMR techniques. The FAD prosthetic group was selectively enriched in 13C and 15N isotopes by adding isotopically labeled riboflavin derivatives to the...

journal_title：Biochemistry

authors： Pust S,Vervoort J,Decker K,Bacher A,Müller F

更新日期：1989-01-24 00:00:00

abstract：:A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...

journal_title：Biochemistry

authors： Wang ZF,Yang J,Nie ZQ,Wu M

更新日期：1991-01-29 00:00:00

abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

journal_title：Biochemistry

authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

更新日期：1998-05-12 00:00:00