Purification and characterization of a gamma-like DNA polymerase from Chlamydomonas reinhardtii.

abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

journal_title：Biochemistry

authors： Fede A,Labhardt A,Bannwarth W,Leupin W

更新日期：1991-12-03 00:00:00

abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...

journal_title：Biochemistry

authors： Cao H,Pietrak BL,Grubmeyer C

更新日期：2002-03-12 00:00:00

abstract：:Microfibrils having a diameter of 10-12 nm, found either in association with elastin or independently, are an important component of the extracellular matrix of many tissues. Because isolation of native proteins composing the microfibrils has proven difficult, information on structure/function relationships is limited...

journal_title：Biochemistry

authors： Bashir MM,Abrams WR,Rosenbloom J,Kucich U,Bacarra M,Han MD,Brown-Augsberger P,Mecham R,Rosenbloom J

更新日期：1994-01-18 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...

journal_title：Biochemistry

authors： Weadge JT,Clarke AJ

更新日期：2006-01-24 00:00:00

abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...

journal_title：Biochemistry

authors： Allocatelli CT,Cutruzzolà F,Brancaccio A,Brunori M,Qin J,La Mar GN

更新日期：1993-06-15 00:00:00

abstract：:Suberyldicholine-induced ion translocation in the millisecond time region in acetylcholine receptor rich membrane vesicles prepared from the electric organ of Electrophorus electricus was investigated in eel Ringer's solution, pH 7.0, 1 degree C. A quench-flow technique with a time resolution of 5 ms was used to measu...

journal_title：Biochemistry

authors： Pasquale EB,Takeyasu K,Udgaonkar JB,Cash DJ,Severski MC,Hess GP

更新日期：1983-12-06 00:00:00

abstract：:We have examined the conformational behavior under various unfolding conditions of a predominantly beta-sheet protein, cellular retinoic acid binding protein (CRABP). Urea unfolding-refolding of CRABP is a highly cooperative process that can be approximated by a two-state model. Acid denaturation is also cooperative a...

journal_title：Biochemistry

authors： Liu ZP,Rizo J,Gierasch LM

更新日期：1994-01-11 00:00:00

abstract：:Primary kinetic isotope effects on Vmax were observed in both reaction directions upon racemizing samples of [2-2H]glutamate with the cofactor-independent glutamate racemase from Lactobacillus. This supports a deprotonation/protonation mechanism for racemization in which the breaking of the carbon-hydrogen bond at C-2...

journal_title：Biochemistry

authors： Tanner ME,Gallo KA,Knowles JR

更新日期：1993-04-20 00:00:00

abstract：:Glycosylases specifically recognize and flip their target base out of the DNA helix into the enzyme's active site. Our simulations show that a partially flipped state, already present in free DNA carrying a T:G mispair, becomes the more probable state compared to the closed state after binding of thymine DNA glycosyla...

journal_title：Biochemistry

authors： Kanaan N,Imhof P

更新日期：2018-10-02 00:00:00

abstract：:Endogenous protein kinase inhibitors are essential for a wide range of physiological functions. These endogenous inhibitors may mimic peptide substrates as in the case of the heat-stable protein kinase inhibitor (PKI), or they may mimic nucleotide triphosphates. Natural product inhibitors, endogenous to the unique org...

journal_title：Biochemistry

authors： Narayana N,Diller TC,Koide K,Bunnage ME,Nicolaou KC,Brunton LL,Xuong NH,Ten Eyck LF,Taylor SS

更新日期：1999-02-23 00:00:00

abstract：:The (+)-trans-anti-benzo[a]pyrene adduct formed at the N2 amino group of guanine is the major adduct found after metabolic activation of the ubiquitous carcinogen benzo[a]pyrene. The carcinogenic and mutagenic properties of the (+)-trans-anti-BP adduct, as well as related adducts, have been extensively studied. A DNA ...

journal_title：Biochemistry

authors： Fountain MA,Krugh TR

更新日期：1995-03-14 00:00:00

abstract：:A structural understanding of the nature and scope of serpin inhibition mechanisms has been limited by the inability so far to crystallize any serpin-proteinase complex. We describe here the application of [(1)H-(15)N]-HSQC NMR on uniformly and residue-selectively (15)N-labeled serpin alpha(1)-proteinase inhibitor (Pi...

journal_title：Biochemistry

authors： Peterson FC,Gordon NC,Gettins PG

更新日期：2000-10-03 00:00:00

abstract：:The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...

journal_title：Biochemistry

authors： Khouri HE,Vernet T,Ménard R,Parlati F,Laflamme P,Tessier DC,Gour-Salin B,Thomas DY,Storer AC

更新日期：1991-09-17 00:00:00

abstract：:Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dim...

journal_title：Biochemistry

authors： Pocanschi CL,Dahmane T,Gohon Y,Rappaport F,Apell HJ,Kleinschmidt JH,Popot JL

更新日期：2006-11-28 00:00:00

abstract：:The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate gro...

journal_title：Biochemistry

authors： Huddleston JP,Raushel FM

更新日期：2019-09-17 00:00:00

abstract：:Kinetic parameters for triosephosphate isomerase catalysis of the elimination reaction of an equilibrium mixture of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (DGAP) to form methylglyoxal and phosphate ion are reported for the enzyme from rabbit muscle. Pseudo-first-order rate constants for the...

journal_title：Biochemistry

authors： Richard JP

更新日期：1991-05-07 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:The effect of substituting Pro-L209 with Tyr, Phe, Glu, and Thr in photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides was investigated by monitoring the light-induced FTIR absorption changes associated with the photoreduction of the secondary quinone Q(B). Pro-L209 is close to a chain of ordered water ...

journal_title：Biochemistry

authors： Nabedryk E,Breton J,Sebban P,Baciou L

更新日期：2003-05-20 00:00:00

abstract：:Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...

journal_title：Biochemistry

authors： El Kirat K,Dufrêne YF,Lins L,Brasseur R

更新日期：2006-08-01 00:00:00

abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...

journal_title：Biochemistry

authors： Sigfridsson K,Young S,Hansson O

更新日期：1996-01-30 00:00:00

abstract：:The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydisper...

journal_title：Biochemistry

authors： Linhardt RJ,Merchant ZM,Rice KG,Kim YS,Fitzgerald GL,Grant AC,Langer R

更新日期：1985-12-17 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...

journal_title：Biochemistry

authors： Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

更新日期：2001-09-11 00:00:00

abstract：:Mixtures of 1,2-dipalmitoylglycerol (1,2-DPG), 1,2-dioleylglycerol (1,2-DOG), 1,2-dicapryloylglycerol (1,2-DCG), 1,3-dioleylglycerol (1,3-DOG), and 1,3-dicapryloylglycerol (1,3-DCG) with dipalmitoylphosphatidylcholine (DPPC) have been studied by means of differential scanning calorimetry (DSC) and fluorescence depolar...

journal_title：Biochemistry

authors： Ortiz A,Villalaín J,Gómez-Fernández JC

更新日期：1988-12-13 00:00:00

abstract：:The stoichiometry of the nitrogenase ATP-dependent H2 evolution and ecetylene reduction reactions using S2O4(2-) as an electron source was studied by various techniques. For each mole of S2O4(2-) oxidized to 2SO3(2-) by the enzyme-catalyzed reactions at 25 degrees and pH 8, 1 mol of H2 (1 mol of ethylene for acetylene...

journal_title：Biochemistry

authors： Watt GD,Bulen WA,Burns A,Hadfield KL

更新日期：1975-09-23 00:00:00

abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...

journal_title：Biochemistry

authors： Dunford HB,Hewson WD

更新日期：1977-06-28 00:00:00

abstract：:Nuclear magnetic resonance (NMR) (15)N relaxation methods have been used to characterize the backbone dynamics of the N-terminal core domain of the HIV-1 capsid protein (CA(151)). The domain, which has an unusually flat, triangular shape, tumbles in solution at 28 degrees C with an effective rotational correlation tim...

journal_title：Biochemistry

authors： Campos-Olivas R,Summers MF

更新日期：1999-08-10 00:00:00

abstract：:Lactate dehydrogenase (LDH) catalyzes the interconversion between pyruvate and lactate with nicotinamide adenine dinucleotide (NAD) as a cofactor. Using isotope-edited difference Fourier transform infrared spectroscopy on the "live" reaction mixture (LDH·NADH·pyruvate ⇌ LDH·NAD(+)·lactate) for the wild-type protein an...

journal_title：Biochemistry

authors： Peng HL,Deng H,Dyer RB,Callender R

更新日期：2014-03-25 00:00:00

abstract：:Inhibition of gamma-aminobutyric acid aminotransferase (GABA-AT) increases the concentration of GABA, an inhibitory neurotransmitter in human brain, which could have therapeutic applications for a variety of neurological diseases, including epilepsy. On the basis of studies of several previously synthesized conformati...

journal_title：Biochemistry

authors： Wang Z,Yuan H,Nikolic D,Van Breemen RB,Silverman RB

更新日期：2006-12-05 00:00:00

abstract：:Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...

journal_title：Biochemistry

authors： Li J,Xiong Y,Bigelow DJ,Squier TC

更新日期：2004-01-20 00:00:00