The SIV tilted peptide induces cylindrical reverse micelles in supported lipid bilayers.

abstract：:Steroidogenic acute regulatory protein (StAR) rapidly stimulates the movement of cholesterol into adrenal and gonadal mitochondria to mediate the acute steroidogenic response; StAR mutations cause potentially lethal congenital lipoid adrenal hyperplasia (lipoid CAH). Bacterially expressed wild-type StAR and four amino...

journal_title：Biochemistry

authors： Bose HS,Baldwin MA,Miller WL

更新日期：1998-07-07 00:00:00

abstract：:Experiments were performed to elucidate the mechanism of hydrocarbon formation in microsomal preparations from the house fly, Musca domestica. Antibody to both house fly cytochrome P450 reductase and a purified cytochrome P450 (CYP6A1) from the house fly inhibited (Z)-9-tricosene (Z9-23:Hy) formation from [15,16-3H]-(...

journal_title：Biochemistry

authors： Reed JR,Quilici DR,Blomquist GJ,Reitz RC

更新日期：1995-12-12 00:00:00

abstract：:The periplasmic nitrate reductase (NapAB), a member of the DMSO reductase superfamily, catalyzes the first step of the denitrification process in bacteria. In this heterodimer, a di-heme NapB subunit is associated to the catalytic NapA subunit that binds a [4Fe-4S] cluster and a bis(molybdopterin guanine dinucleotide)...

journal_title：Biochemistry

authors： Dementin S,Arnoux P,Frangioni B,Grosse S,Léger C,Burlat B,Guigliarelli B,Sabaty M,Pignol D

更新日期：2007-08-28 00:00:00

abstract：:The subunit structure of Hsp80, the most abundant heat-shock protein of Neurospora crassa, was examined by chemical cross-linking of the purified protein in vitro. Resolution of glutaraldehyde-treated Hsp80 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE suggests that the native state of this pro...

journal_title：Biochemistry

authors： Freitag DG,Ouimet PM,Girvitz TL,Kapoor M

更新日期：1997-08-19 00:00:00

abstract：:Room temperature phosphorescence techniques were used to study the structural and dynamic features of the tryptophan residues in bovine alpha-crystallin. Upon excitation at 290 nm, the characteristic signature of tryptophan phosphorescence was observed with an emission maximum at 442 +/- 2 nm. The phosphorescence inte...

journal_title：Biochemistry

authors： Berger JW,Vanderkooi JM

更新日期：1989-06-27 00:00:00

abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...

journal_title：Biochemistry

authors： Króliczewski J,Hombek-Urban K,Szczepaniak A

更新日期：2005-05-24 00:00:00

abstract：:In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...

journal_title：Biochemistry

authors： Anders J,Blüggel M,Meyer HE,Kühne R,ter Laak AM,Kojro E,Fahrenholz F

更新日期：1999-05-11 00:00:00

abstract：:The kinetic and thermodynamic features of reactions catalyzed by present-day enzymes appear to be the consequence of the evolution of these proteins toward maximal catalytic effectiveness. These features are identified and analyzed (in detail for one substrate-one product enzymes) by using ideas that link the energeti...

journal_title：Biochemistry

authors： Burbaum JJ,Raines RT,Albery WJ,Knowles JR

更新日期：1989-11-28 00:00:00

abstract：:Both agonist- and antagonist-bound glucocorticoid receptors (GRs) and progesterone receptors (PRs) regulate gene transcription with the assistance of corepressors (NCoR and SMRT) and coactivators (TIF2/GRIP1, SRC1, and AIB1). Receptor binding of these cofactors is competitive and is considered to involve interactions ...

journal_title：Biochemistry

authors： Wang D,Wang Q,Awasthi S,Simons SS Jr

更新日期：2007-07-10 00:00:00

abstract：:Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...

journal_title：Biochemistry

authors： Ohya M,Nishitani C,Sano H,Yamada C,Mitsuzawa H,Shimizu T,Saito T,Smith K,Crouch E,Kuroki Y

更新日期：2006-07-18 00:00:00

abstract：:NMR studies of the adenosine analog tubercidin have been carried out in the presence of Escherichia coli purine nucleoside phosphorylase (PNP) in order to characterize the conformation of the enzyme-complexed nucleoside. Although analysis of transferred NOE data at various enzyme/inhibitor ratios indicated a predomina...

journal_title：Biochemistry

authors： Perlman ME,Davis DG,Koszalka GW,Tuttle JV,London RE

更新日期：1994-06-21 00:00:00

abstract：:Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...

journal_title：Biochemistry

authors： Nolte WM,Tagore DM,Lane WS,Saghatelian A

更新日期：2009-12-22 00:00:00

abstract：:Neutrophil elastase (NE), a mediator of inflammation, binds with high affinity numerous anionic molecules including suramin, a polysulfated naphthylurea, which inhibits it with a Ki of 0.2 microM and a 4:1 suramin:NE stoichiometry and thus constitutes a potential therapeutic agent. In an attempt to locate the suramin ...

journal_title：Biochemistry

authors： Mély Y,Cadène M,Sylte I,Bieth JG

更新日期：1997-12-16 00:00:00

abstract：:The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...

journal_title：Biochemistry

authors： Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

更新日期：2009-12-29 00:00:00

abstract：:Peripheral benzodiazepine receptors (PBR), first described more than 20 years ago, have been attributed with many putative functions including ones in cellular proliferation and cellular respiration. Hence, it is quite conceivable that deregulation of this receptor could lead to pathology. We and others have reported ...

journal_title：Biochemistry

authors： Weisinger G,Kelly-Hershkovitz E,Veenman L,Spanier I,Leschiner S,Gavish M

更新日期：2004-09-28 00:00:00

abstract：:The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known regarding the role of these interactions in the mechanism of MC4R activation...

journal_title：Biochemistry

authors： Fleck BA,Ling N,Chen C

更新日期：2007-09-18 00:00:00

abstract：:To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with...

journal_title：Biochemistry

authors： Lofstad M,Gudim I,Hammerstad M,Røhr ÅK,Hersleth HP

更新日期：2016-09-13 00:00:00

abstract：:NMR spectroscopy is particularly adept at site-specifically monitoring dynamic processes in proteins, such as protein folding, domain movements, ligand binding, and side-chain rotations. By coupling the favorable spectroscopic properties of highly dynamic side-chain methyl groups with transverse-relaxation-optimized s...

journal_title：Biochemistry

authors： Boswell ZK,Latham MP

更新日期：2019-01-22 00:00:00

abstract：:Oxygen and nitroaromatic compounds are known to enhance the sensitivity of cells to ionizing radiation. Employing calf thymus DNA and oligo(dA)12/poly(dT), we have examined the differences to DNA damage, in particular thymine glycols and the 3'-DNA termini at strand breaks, arising from irradiation under anoxic and ox...

journal_title：Biochemistry

authors： Buchko GW,Weinfeld M

更新日期：1993-03-09 00:00:00

abstract：:Single-molecule fluorescence resonance energy transfer (smFRET) methods were used to study the assembly pathway and DNA unwinding activity of the bacteriophage T4 helicase-primase (primosome) complex. The helicase substrates used were surface-immobilized model DNA replication forks "internally" labeled in the duplex r...

journal_title：Biochemistry

authors： Lee W,Jose D,Phelps C,Marcus AH,von Hippel PH

更新日期：2013-05-07 00:00:00

abstract：:Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...

journal_title：Biochemistry

authors： Schonhoft JD,Stivers JT

更新日期：2013-04-16 00:00:00

abstract：:During DNA replication, the presence of oligonucleotides with partial homology to the template strand was shown to induce a switch of the polymerase from the normal template to the oligonucleotide. The latter acted as a dead-end template and led to abortive replication. The only prerequisite was that the oligonucleoti...

journal_title：Biochemistry

authors： Guieysse AL,Praseuth D,Grigoriev M,Harel-Bellan A,Hélène C

更新日期：1995-07-18 00:00:00

abstract：:The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...

journal_title：Biochemistry

authors： Yakovlev S,Gorlatov S,Ingham K,Medved L

更新日期：2003-07-01 00:00:00

abstract：:NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a highly complicated, energy transducing, membrane-bound enzyme. It contains 46 different subunits and nine redox cofactors: a noncovalently bound flavin mononucleotide and eight iron-sulfur clusters. The mechanism of complex I is not known. ...

journal_title：Biochemistry

authors： Sharpley MS,Shannon RJ,Draghi F,Hirst J

更新日期：2006-01-10 00:00:00

abstract：:The molecular mechanism of O2 binding to hemoglobin (Hb) and myoglobin (Mb) is a long-standing issue in the field of bioinorganic and biophysical chemistry. The nature of Fe-O2 bond in oxy Hb and Mb had been extensively investigated by resonance Raman spectroscopy, which assigned the Fe-O2 stretching bands at ∼570 cm-...

journal_title：Biochemistry

authors： Ohta T,Shibata T,Kobayashi Y,Yoda Y,Ogura T,Neya S,Suzuki A,Seto M,Yamamoto Y

更新日期：2018-12-04 00:00:00

abstract：:The binding of Zn2+ to tubulin and the ability of this cation to promote the polymorphic assembly of the protein were examined. Equilibrium binding showed the existence of more than 60 potential Zn2+ binding sites on the dimer, including a number of high-affinity sites. The number of high-affinity sites, estimated by ...

journal_title：Biochemistry

authors： Eagle GR,Zombola RR,Himes RH

更新日期：1983-01-04 00:00:00

abstract：:Insulin decreases multifunctional protein kinase (MFPK) activity in rat adipose tissue [Ramakrishna, S., & Benjamin, W. B. (1988) J. Biol. Chem. 263, 12677-12681]. Insulin also decreases the phosphorylation of peptide B but increases the phosphorylation of peptide A of ATP-citrate lyase (ATP-CL). The mechanism for thi...

journal_title：Biochemistry

authors： Ramakrishna S,Murthy KS,Benjamin WB

更新日期：1989-01-24 00:00:00

abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...

journal_title：Biochemistry

authors： Zoldák G,Sedlák E,Wolfrum A,Musatov A,Fedunová D,Szkaradkiewicz K,Sprinzl M

更新日期：2008-04-29 00:00:00

abstract：:Antithrombin III (ATIII) is a plasma-borne serine protease inhibitor that apparently forms covalent complexes with thrombin. The interaction between ATIII and thrombin is enhanced several thousandfold by the glycosaminoglycan, heparin. We have previously proposed that the heparin binding site of ATIII resides within a...

journal_title：Biochemistry

authors： Smith JW,Dey N,Knauer DJ

更新日期：1990-09-25 00:00:00

abstract：:S-Glutathionylation is a redox-regulated modification that uncouples endothelial nitric oxide synthase (eNOS), switching its function from nitric oxide (NO) synthesis to (•)O2(-) generation, and serves to regulate vascular function. While in vitro or in vivo eNOS S-glutathionylation with modification of Cys689 and Cys...

journal_title：Biochemistry

authors： Chen CA,De Pascali F,Basye A,Hemann C,Zweier JL

更新日期：2013-09-24 00:00:00