Abstract：
:Peripheral benzodiazepine receptors (PBR), first described more than 20 years ago, have been attributed with many putative functions including ones in cellular proliferation and cellular respiration. Hence, it is quite conceivable that deregulation of this receptor could lead to pathology. We and others have reported the existence of PBR overexpression in different human and nonhuman malignancies, but it has never been made clear whether this aberrant malignant PBR expression is a cause or consequence of the cancer. In the current study we induced PBR underexpression by downregulating one critical subunit of the PBR complex, the isoquinoline-binding protein (IBP), using the stable antisense knockout approach, in the MA-10 Leydig cell line. Resultant clones, showing PBR deregulation, also demonstrated increased tumorigenicity, using both in vitro (loss of contact inhibition and growth in soft agar) and in vivo (increased mortality on grafting back into isogenic mice) assays. We suggest that this type of deregulation could be a later event in natural tumor progression. Consequently, PBR deregulation should be more closely studied in human malignancy.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Weisinger G,Kelly-Hershkovitz E,Veenman L,Spanier I,Leschiner S,Gavish Mdoi
10.1021/bi030251vsubject
Has Abstractpub_date
2004-09-28 00:00:00pages
12315-21issue
38eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Variations for non-histones in the ADP-ribosylating activities of interphase and metaphase cells were investigated. 32P-Labeled nicotinamide adenine dinucleotide ([32P]NAD), the specific precursor for the modification, was used to radioactively label proteins. Permeabilized interphase and mitotic cells, as well as iso...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00323a017
更新日期：1985-01-15 00:00:00
abstract：:The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00693a014
更新日期：1975-11-04 00:00:00
abstract：:TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a037
更新日期：1993-04-06 00:00:00
abstract：:Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. The magnitude of the SPR spectral changes caused by light varies with pH in a manner par...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00250a022
更新日期：1994-11-22 00:00:00
abstract：:Thermal pertubation difference spectra of sperm whale myoglobin (Mb) and soybean leghemoglobin a (Lb a) in the near-ultraviolet reveal similarities in the tryptophan environment of the two proteins. Of the two tryptophans in each protein, one has its indolyl NH group fully exposed to aqueous solvent, while the other b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00620a009
更新日期：1977-01-11 00:00:00
abstract：:Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00277a020
更新日期：1983-04-12 00:00:00
abstract：:The respiratory chain of Vibrio cholerae contains three bd-type quinol oxygen reductases as well as one cbb(3) oxygen reductase. The cbb(3) oxygen reductase has been previously isolated and characterized; however, the natural mobile electron donor(s) that shuttles electrons between the bc(1) complex and the cbb(3) oxy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1004574
更新日期：2010-09-07 00:00:00
abstract：:Serine hydroxymethyltransferase and C1-tetrahydrofolate synthase catalyze four reactions which convert formate and glycine to serine. The one-carbon carrier in these reactions if tetrahydropteroylglutamate which is regenerated in the coupled reaction and thus can be used in catalytic concentrations with respect to ser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00450a028
更新日期：1989-11-28 00:00:00
abstract：:The zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. Their mechanism is poorly understood, and there are no clinically useful inhibitors. While most members of the group contain two tightly bound zinc ions in their active site...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047709t
更新日期：2005-03-29 00:00:00
abstract：:Transmembrane chemoreceptors, also known as methyl-accepting chemotaxis proteins (MCPs), translate extracellular signals into intracellular responses in the bacterial chemotaxis system. MCP ligand binding domains control the activity of the CheA kinase, situated approximately 200 A away, across the cytoplasmic membran...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801727m
更新日期：2009-03-10 00:00:00
abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a011
更新日期：1978-10-31 00:00:00
abstract：:ATP-dependent Mg2+ accumulation in isolated mitochondria occurs predominantly in the matrix and inner membrane compartments. In mitochondria contaminated with lysosomes, the time course and magnitude of ATP-dependent Mg2+ accumulation are influenced by various cytoplasmic substances, besides substrates of the citric a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00656a013
更新日期：1976-06-01 00:00:00
abstract：:A recirculating Langendorff heart preparation is used to characterize the endogenous antithrombin associated reversibly with murine vascular endothelium. Rat hearts are perfused clear of blood and then recirculated with a physiological salt solution. Addition of heparin educes antithrombin activity continuously into t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00169a024
更新日期：1994-01-25 00:00:00
abstract：:Fibronectin is composed of multiple homologous repeats and contains many functional domains. Two major heparin-binding domains have previously been identified: the Hep I site near the amino terminus and the Hep II site near the carboxyl terminus. The Hep II site has been considered the high-affinity heparin-binding si...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991624o
更新日期：2000-03-28 00:00:00
abstract：:Yeast mitochondrial NAD+-specific isocitrate dehydrogenase (IDH) has previously been shown to bind specifically to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions have been found to allosterically inhibit activity of the enzyme. This inhibition is relieved by AMP, an allo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0200662
更新日期：2002-06-04 00:00:00
abstract：:The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9922547
更新日期：2000-02-15 00:00:00
abstract：:Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0026324
更新日期：2001-02-27 00:00:00
abstract：:Phosphoinositide 3-kinase gamma (PI3Kgamma) is activated by Gbetagamma release after stimulation of Galpha i -coupled receptors, involving a recruitment of the enzyme to the plasma membrane via interaction of the regulatory subunit p101 or p87 with Gbetagamma. The receptor-mediated release of Gbetagamma was, however, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800596b
更新日期：2008-10-28 00:00:00
abstract：:The kinetics of the phospholipase A2-catalyzed hydrolysis of bilayer vesicles and mixed micelles of several oxyglycero and thioglycero analogues of phospholipids have been studied. The results with vesicles show that, depending on the source of the enzyme, the rates of hydrolysis of the oxy-containing long-chain phosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00149a014
更新日期：1992-09-01 00:00:00
abstract：:The gel to liquid-crystalline phase transition temperatures of dispersions of mixed-acid sn-1,2-lecithins which contain one unsaturated and one saturated fatty acid have been studied by differential scanning calorimetry. The temperature for 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (containing no reversed isome...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00515a051
更新日期：1981-06-09 00:00:00
abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00549a025
更新日期：1980-04-15 00:00:00
abstract：:The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201603f
更新日期：2012-01-10 00:00:00
abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a038
更新日期：1985-04-09 00:00:00
abstract：:The recognition sequence of the Myb protein has been recently described to be pyAACKGHH (where py = T/C, K = G/T, and H = A/C/T), modifying the earlier identification as pyAACKG [Ording, E., et al. (1994) Eur. J. Biochem. 222, 113-120]. We had earlier determined the solution structure of the minimal cognate sequence T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9806753
更新日期：1998-07-14 00:00:00
abstract：:The synthesis of two potential arginine-directed purine-based analogues, 6-chloro-9-(3,4-dioxopentyl)purine (6) and 9-(3,4-dioxopentyl)hypoxanthine (7), is reported. Compound 7 was extensively tested as a potential affinity label of purine nucleoside phosphorylase (EC 2.4.2.1) from human erythrocytes. Evidence that 7 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00268a010
更新日期：1982-12-07 00:00:00
abstract：:Chemical modification, mutagenesis, chemical rescue, and isotope effect studies are used to identify and probe the roles of several conserved amino acid groups in catalysis by human dihydroorotate dehydrogenase. Time- and pH-dependent inactivation of human dihydroorotate dehydrogenase by trinitrobenzenesulfonate impli...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000630d
更新日期：2000-07-11 00:00:00
abstract：:Three different cleavable photoactivable heterobifunctional reagents have been synthesized and characterized by proton NMR, 13C NMR, mass spectroscopy, infrared spectroscopy, and thin-layer chromatography. They are the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]-propionic acid, methyl 3-[(4-azidophenyl)dit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00527a003
更新日期：1981-11-24 00:00:00
abstract：:Pluronic block copolymers (triblock copolymers of poly(ethylene oxide) and poly(propylene oxide)) exhibit a chemosensitizing effect on multidrug resistant cell lines. Changes in membrane permeability are hypothesized to be responsible because inhibition of drug transport mediated by both the multidrug-resistance-assoc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035768l
更新日期：2004-03-30 00:00:00
abstract：:Di-leucine motifs have been implicated in the internalization or degradation of many membrane proteins. The epidermal growth factor receptor (EGFR) contains two di-leucine residues at 658 (TLRRLLQER) and 679 (NQALLRIL). To determine the role of these di-leucine motifs in regulating EGF receptor expression, activity, o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961630+
更新日期：1996-11-19 00:00:00
abstract：:Human placental lipocortin is a high-affinity substrate for rat brain protein kinase C in vitro with phosphorylation occurring on serine and threonine residues in a ratio of approximately 2 to 1. Comparison of the ability of various N-terminal-truncated derivatives of lipocortin to serve as phosphorylation substrates,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00412a008
更新日期：1988-06-14 00:00:00
