Characterization of lens alpha-crystallin tryptophan microenvironments by room temperature phosphorescence spectroscopy.

abstract：:The aspartate receptor is one of the ligand-specific, homodimeric chemoreceptors that detects extracellular attractants and triggers the chemotaxis pathway of Escherichia coli and Salmonella typhimurium. This receptor regulates the activity of the histidine kinase CheA, which forms a kinetically stable complex with th...

journal_title：Biochemistry

authors： Winston SE,Mehan R,Falke JJ

更新日期：2005-09-27 00:00:00

abstract：:The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...

journal_title：Biochemistry

authors： Campbell JC,Suelter CH

更新日期：1977-11-01 00:00:00

abstract：:Ca2+-free crystals of sarcoplasmic reticulum Ca2+-ATPase have, up until now, been obtained in the presence of inhibitors such as thapsigargin (TG), bound to the transmembrane region of this protein. Here, we examined the consequences of such binding for the protein. We found that, after TG binding, an active site liga...

journal_title：Biochemistry

authors： Montigny C,Picard M,Lenoir G,Gauron C,Toyoshima C,Champeil P

更新日期：2007-12-25 00:00:00

abstract：:The calcium binding protein alpha-lactalbumin folds via a molten globule intermediate. Calcium does not bind strongly to the unfolded protein or the molten globule, but does bind to the transition state between the molten globule and the native protein. Of interest are the structures formed in the transition state tha...

journal_title：Biochemistry

authors： Kuhlman B,Boice JA,Wu WJ,Fairman R,Raleigh DP

更新日期：1997-04-15 00:00:00

abstract：:Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

journal_title：Biochemistry

authors： Aitken SM,Kim DH,Kirsch JF

更新日期：2003-09-30 00:00:00

abstract：:Retinoid X receptors (RXRs) are transcription factors with important functions in embryonic development, metabolic processes, differentiation, and apoptosis. A particular feature of RXRs is their ability to act as obligatory heterodimerization partners of class II nuclear receptors. At the same time, these receptors a...

journal_title：Biochemistry

authors： Belorusova A,Osz J,Petoukhov MV,Peluso-Iltis C,Kieffer B,Svergun DI,Rochel N

更新日期：2016-03-29 00:00:00

abstract：:FeNO vibrational frequencies were investigated for a series of myoglobin mutants using isotope-edited resonance Raman spectra of (15/14)NO adducts, which reveal the FeNO and NO stretching modes. The latter give rise to doublet bands, as a result of Fermi resonances with coincident porphyrin vibrations; these doublets ...

journal_title：Biochemistry

authors： Coyle CM,Vogel KM,Rush TS 3rd,Kozlowski PM,Williams R,Spiro TG,Dou Y,Ikeda-Saito M,Olson JS,Zgierski MZ

更新日期：2003-05-06 00:00:00

abstract：:The complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) (EC 2.6.1.2) is presented. Two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and Achromobacter protease I, respectively. Isolated...

journal_title：Biochemistry

authors： Ishiguro M,Takio K,Suzuki M,Oyama R,Matsuzawa T,Titani K

更新日期：1991-10-29 00:00:00

abstract：:For some hormone receptors, the early events of signal transduction depend on their molecular arrangement and interactions at the cell surface. An understanding of the mechanism of signal transduction in general needs a careful analysis of the receptor distribution. Here, we present the first quantitative measurement ...

journal_title：Biochemistry

authors： St-Pierre PR,Petersen NO

更新日期：1992-03-10 00:00:00

abstract：:A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...

journal_title：Biochemistry

authors： Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

更新日期：1989-10-03 00:00:00

abstract：:Extended-spectrum β-lactamases (ESBLs) pose a threat to public health because of their ability to confer resistance to extended-spectrum cephalosporins such as cefotaxime. The CTX-M β-lactamases are the most widespread ESBL enzymes among antibiotic resistant bacteria. Many of the active site residues are conserved bet...

journal_title：Biochemistry

authors： Adamski CJ,Cardenas AM,Brown NG,Horton LB,Sankaran B,Prasad BV,Gilbert HF,Palzkill T

更新日期：2015-01-20 00:00:00

abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...

journal_title：Biochemistry

authors： Mishra VK,Palgunachari MN,Datta G,Phillips MC,Lund-Katz S,Adeyeye SO,Segrest JP,Anantharamaiah GM

更新日期：1998-07-14 00:00:00

abstract：:The interfacial interactions of cholesterol with sphingomyelins (SMs) containing various homogeneous acyl chains have been investigated by Langmuir film balance approaches. Low in-plane elasticity among the packed lipids was identified as an important physical feature of the cholesterol-sphingomyelin liquid-ordered ph...

journal_title：Biochemistry

authors： Li XM,Momsen MM,Smaby JM,Brockman HL,Brown RE

更新日期：2001-05-22 00:00:00

abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...

journal_title：Biochemistry

authors： Haapalainen AM,Meriläinen G,Pirilä PL,Kondo N,Fukao T,Wierenga RK

更新日期：2007-04-10 00:00:00

abstract：:The first structure of a glycerol kinase from a Gram-positive organism, Enterococcus casseliflavus, has been determined to 2.8 A resolution in the presence of glycerol and to 2.5 A resolution in the absence of substrate. The substrate-induced closure of 7 degrees is significantly smaller than that reported for hexokin...

journal_title：Biochemistry

authors： Yeh JI,Charrier V,Paulo J,Hou L,Darbon E,Claiborne A,Hol WG,Deutscher J

更新日期：2004-01-20 00:00:00

abstract：:35S-labeled derivatives of the insecticides nodulisporic acid and ivermectin were synthesized and demonstrated to bind with high affinity to a population of receptors in Drosophila head membranes that were previously shown to be associated with a glutamate-gated chloride channel. Nodulisporic acid binding was modeled ...

journal_title：Biochemistry

authors： Ludmerer SW,Warren VA,Williams BS,Zheng Y,Hunt DC,Ayer MB,Wallace MA,Chaudhary AG,Egan MA,Meinke PT,Dean DC,Garcia ML,Cully DF,Smith MM

更新日期：2002-05-21 00:00:00

abstract：:Conformations of (R)-alpha-(N6-adenyl)styrene oxide adducts at positions X6 in d(CGGACXAGAAG).d(CTTCTTGTCCG) and X7 in d(CGGACAXGAAG).d(CTTCTTGTCCG), incorporating codons 60, 61 (underlined), and 62 of the human n-ras protooncogene, were refined from 1H NMR data. These were the R(61,2) and R(61,3) adducts. Chemical sh...

journal_title：Biochemistry

authors： Feng B,Zhou L,Passarelli M,Harris CM,Harris TM,Stone MP

更新日期：1995-10-31 00:00:00

abstract：:This paper contributes to the characterization of partial steps of electron and proton transfer in mitochondrial cytochrome c oxidase with respect to their membrane arrangement and involvement in energy-linked protonmotive activity. It is shown that delta psi controls electron flow from cytochrome c to heme a is consi...

journal_title：Biochemistry

authors： Capitanio N,De Nitto E,Villani G,Capitanio G,Papa S

更新日期：1990-03-27 00:00:00

abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

journal_title：Biochemistry

authors： Kuhlmann J,Macara I,Wittinghofer A

更新日期：1997-10-07 00:00:00

abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

journal_title：Biochemistry

authors： Claffey WJ,Holzbach RT

更新日期：1981-01-20 00:00:00

abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

journal_title：Biochemistry

authors： Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

更新日期：1985-04-09 00:00:00

abstract：:The packing of lipids into different aggregates, such as spheres, rods, or bilayers, is dependent on the hydrophobic volume, the hydrocarbon-water interfacial area, and the hydrocarbon chain length of the participating molecules, according to the self-assembly theory [Israelachvili, J. N., Marcelja, S., & Horn, R. G. ...

journal_title：Biochemistry

authors： Wieslander A,Rilfors L,Lindblom G

更新日期：1986-11-18 00:00:00

abstract：:Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...

journal_title：Biochemistry

authors： Stroop SD,Kuestner RE,Serwold TF,Chen L,Moore EE

更新日期：1995-01-24 00:00:00

abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...

journal_title：Biochemistry

authors： He Y,Luo Z,Klekotka PA,Burnett VL,Halpert JR

更新日期：1994-04-12 00:00:00

abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

journal_title：Biochemistry

authors： Cech CL,McClure WR

更新日期：1980-05-27 00:00:00

abstract：:The interaction of Triton X-100 and other nonionic detergents with a delipidated preparation of the Ca2+ ATPase from sarcoplasmic reticulum has been studied. Binding of radiolabeled Triton X-100 was determined by column chromatography at 6 degrees C, and two classes of binding sites were observed. Below the critical m...

journal_title：Biochemistry

authors： Dean WL,Suárez CP

更新日期：1981-03-31 00:00:00

abstract：:The majority of pre-steady-state kinetic investigations with HIV-1 reverse transcriptase (HIV-1 RT) have reported substoichiometric bursts (30-50%) of product formation in the initial reaction cycle. By using quantitative amino acid analysis, we have revised the extinction coefficient of the HIV-1 RT heterodimer and s...

journal_title：Biochemistry

authors： Furge LL,Guengerich FP

更新日期：1999-04-13 00:00:00

abstract：:Although plasma membrane (PM) cholesterol-rich and -poor domains have been isolated by subcellular fractionation, the real-time arrangement of cholesterol in such domains in living cells is still unclear. Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cel...

journal_title：Biochemistry

authors： Zhang W,McIntosh AL,Xu H,Wu D,Gruninger T,Atshaves B,Liu JC,Schroeder F

更新日期：2005-03-01 00:00:00

abstract：:HPr is a protein of the bacterial phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In Gram-positive bacteria, HPr can be phosphorylated on Ser(46) by HPr(Ser) kinase/phosphorylase (HPrK/P) and on His(15) by enzyme I (EI) of the PTS. In vitro studies have shown that phosphorylation on one residue gr...

journal_title：Biochemistry

authors： Casabon I,Couture M,Vaillancourt K,Vadeboncoeur C

更新日期：2006-05-30 00:00:00

abstract：:Complete resonance assignments of nonexchangeable base protons and sugar protons in d-GGTACGCGTACC at 500 MHz have been obtained by two-dimensional correlated spectroscopy (COSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The characteristic phase-sensitive multiplet patterns of the ntrasugar cross peaks ...

journal_title：Biochemistry

authors： Chary KV,Hosur RV,Govil G,Chen CQ,Miles HT

更新日期：1988-05-17 00:00:00