Average density and size of microclusters of epidermal growth factor receptors on A431 cells.

abstract：:Alpha-synuclein (alphaS) is a lipid-binding synaptic protein of unknown function that is found in an aggregated amyloid fibril form in the intraneuronal Lewy body deposits that are a defining characteristic of Parkinson's disease (PD). Although intrinsically unstructured when free in solution, alphaS adopts a highly h...

journal_title：Biochemistry

authors： Bussell R Jr,Eliezer D

更新日期：2004-04-27 00:00:00

abstract：:Nitric oxide (NO) regulates cardiovascular hemostasis by binding to soluble guanylyl cyclase (sGC), leading to cGMP production, reduced cytosolic calcium concentration ([Ca(2+)](i)), and vasorelaxation. Thrombospondin-1 (TSP-1), a secreted matricellular protein, was recently discovered to inhibit NO signaling and sGC ...

journal_title：Biochemistry

authors： Ramanathan S,Mazzalupo S,Boitano S,Montfort WR

更新日期：2011-09-13 00:00:00

abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

journal_title：Biochemistry

authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

更新日期：2002-01-15 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

journal_title：Biochemistry

authors： Truong TH,Carroll KS

更新日期：2012-12-18 00:00:00

abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

journal_title：Biochemistry

authors： Olesen K,Andréasson LE

更新日期：2003-02-25 00:00:00

abstract：:The site-specific chemical modification of NADH-quinone oxidoreductase (complex I) by various functional probes such as fluorophores and microbeads, without affecting the enzyme activity, may allow single-molecule analyses of putative dynamic conformational changes in the enzyme. In an attempt to address this challeng...

journal_title：Biochemistry

authors： Masuya T,Murai M,Ifuku K,Morisaka H,Miyoshi H

更新日期：2014-04-15 00:00:00

abstract：:The zinc-endopeptidase light chain of botulinum A neurotoxin undergoes autocatalytic fragmentation that is accelerated by the presence of the metal cofactor, zinc [Ahmed, S. A. et al. (2001) J. Protein Chem. 20, 221-231]. We show in this paper that >95% fragmented light chain obtained in the absence of added zinc reta...

journal_title：Biochemistry

authors： Ahmed SA,McPhie P,Smith LA

更新日期：2003-11-04 00:00:00

abstract：:The sequences and three-dimensional structures of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family were compared to identify highly conserved surface residues. The functions of these solvent-accessible residues were assessed by determining the effects of their substit...

journal_title：Biochemistry

authors： Andreassi JL 2nd,Leyh TS

更新日期：2004-11-23 00:00:00

abstract：:Class A beta-lactamases hydrolyze penicillins and other beta-lactams via an acyl-enzyme catalytic mechanism. Ser70 is the active site nucleophile. By constructing the S70A mutant, which is unable to form the acyl-enzyme intermediate, it was possible to make stable ES complexes with various substrates. The stability of...

journal_title：Biochemistry

authors： Hokenson MJ,Cope GA,Lewis ER,Oberg KA,Fink AL

更新日期：2000-05-30 00:00:00

abstract：:Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...

journal_title：Biochemistry

authors： Gasselhuber B,Carpena X,Graf MM,Pirker KF,Nicolussi A,Sündermann A,Hofbauer S,Zamocky M,Furtmüller PG,Jakopitsch C,Oostenbrink C,Fita I,Obinger C

更新日期：2015-09-08 00:00:00

abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...

journal_title：Biochemistry

authors： Coughlin RT,Haug A,McGroarty EJ

更新日期：1983-04-12 00:00:00

abstract：:Product distributions for the reaction of glycolaldehyde labeled with carbon-13 at the carbonyl carbon ([1-(13)C]-GA) catalyzed by triosephosphate isomerase (TIM) in D(2)O at pD 7.0 in the presence of phosphite dianion and in its absence were determined by (1)H NMR spectroscopy. We observe three products for the relat...

journal_title：Biochemistry

authors： Go MK,Amyes TL,Richard JP

更新日期：2009-06-23 00:00:00

abstract：:Treatment of the beta 2 adrenergic receptor with the reducing agent dithiothreitol (DTT) is known to abolish ligand binding to the receptor. Interestingly, the loss of binding can be prevented by preoccupation of the receptor with ligand. It is unclear, however, whether the ligand blocks access of DTT to the receptor,...

journal_title：Biochemistry

authors： Lin S,Gether U,Kobilka BK

更新日期：1996-11-19 00:00:00

abstract：:The orientation of eukaryotic cytochromes P450, with respect to the membrane of the endoplasmic reticulum, has been investigated. There is now good evidence that the tertiary structure of these proteins is essentially the same as that of the soluble bacterial isoenzyme cytochrome P450CI, with the exception of an exten...

journal_title：Biochemistry

authors： Edwards RJ,Murray BP,Singleton AM,Boobis AR

更新日期：1991-01-08 00:00:00

abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...

journal_title：Biochemistry

authors： Rogl H,Schödel R,Lokstein H,Kühlbrandt W,Schubert A

更新日期：2002-02-19 00:00:00

abstract：:Experimental measurements of the kinetic mechanism involving isoleucyl-tRNA synthetase proofreading valyl-tRNAIle in Escherichia coli have been incorporated into the conventional Michaelis-Menten model for this system. The model was subjected to a detailed mathematical analysis in the steady state. The results of this...

journal_title：Biochemistry

authors： Okamoto M,Savageau MA

更新日期：1984-04-10 00:00:00

abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...

journal_title：Biochemistry

authors： Morita I,Smith WL,DeWitt DL,Schindler M

更新日期：1995-05-30 00:00:00

abstract：:Ca2+-induced fusion of phospholipid vesicles (phosphatidylcholine/phosphatidic acid, 9:1 mol/mol) prepared by ethanolic injection was followed by five different procedures: resonance energy transfer, light scattering, electron microscopy, intermixing of aqueous content, and gel filtration through Sepharose 4-B. The fi...

journal_title：Biochemistry

authors： Morero RD,Viñals AL,Bloj B,Farías RN

更新日期：1985-04-09 00:00:00

abstract：:In globular proteins, there are intermolecular hydrogen bonds between protein and water molecules, and between water molecules, which are bound with the proteins, in addition to intramolecular hydrogen bonds. To estimate the contribution of these hydrogen bonds to the conformational stability of a protein, the thermod...

journal_title：Biochemistry

authors： Takano K,Yamagata Y,Funahashi J,Hioki Y,Kuramitsu S,Yutani K

更新日期：1999-09-28 00:00:00

abstract：:The deposition of amyloid-β (Aβ) plaques and tau-based neurofibrillary tangles is a neuropathological feature of Alzheimer's disease (AD). While studies have shown that the Aβ and tau interaction results in elevated AD pathology, the molecular linkage and mechanism of interaction of Aβ and tau are unclear. A recent st...

journal_title：Biochemistry

authors： Tripathi T,Khan H

更新日期：2020-02-04 00:00:00

abstract：:Known crystal structures of class II aminoacyl-tRNA synthetases complexed to their cognate tRNAs reveal that critical acceptor stem contacts are made by the variable loop connecting the beta-strands of motif 2 located within the catalytic core of class II synthetases. To identify potential acceptor stem contacts made ...

journal_title：Biochemistry

authors： Burke B,Yang F,Chen F,Stehlin C,Chan B,Musier-Forsyth K

更新日期：2000-12-19 00:00:00

abstract：:To explore the interactions of triacylglycerol and phospholipid hydrolysis in lipoprotein conversions and remodeling, we compared the activities of lipoprotein and hepatic lipases on human VLDL, IDL, LDL, and HDL2. Triacylglycerol and phospholipid hydrolysis by each enzyme were measured concomitantly in each lipoprote...

journal_title：Biochemistry

authors： Deckelbaum RJ,Ramakrishnan R,Eisenberg S,Olivecrona T,Bengtsson-Olivecrona G

更新日期：1992-09-15 00:00:00

abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

journal_title：Biochemistry

authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

更新日期：1998-02-24 00:00:00

abstract：:Endonuclease V from bacteriophage T4 is a well characterized enzyme that initiates the repair of ultraviolet light induced pyrimidine dimers. Scission of the phosphodiester backbone between the pyrimidines within a dimer, or 3' to an abasic (AP) site, occurs by a beta-elimination mechanism. In addition, high concentra...

journal_title：Biochemistry

authors： Latham KA,Lloyd RS

更新日期：1995-07-11 00:00:00

abstract：:This study highlights the effect of incorporation of locked nucleic acid (LNA) on improving the functional efficacy of DNAzymes against microRNAs (antagomirzymes). DNAzymes were designed against two different sites of miR-27a, which were encompassed both within the precursor and mature form of miRNA. The cleavage and ...

journal_title：Biochemistry

authors： Kaur H,Scaria V,Maiti S

更新日期：2010-11-09 00:00:00

abstract：:Structural and biochemical characterization of aspartate 152 at the distal heme side of catalase-peroxidase (KatG) from Synechocystis PCC 6803 reveals an important functional role for this residue. In the wild-type protein, the side chain carboxyl group of Asp152 is 7.8 A apart from the heme iron and is hydrogen-bonde...

journal_title：Biochemistry

authors： Jakopitsch C,Auer M,Regelsberger G,Jantschko W,Furtmüller PG,Rüker F,Obinger C

更新日期：2003-05-13 00:00:00

abstract：:A wide variety of biological processes including differentiation, regeneration, and cancer progression are regulated by shedding of membrane-anchored proteins. One of the major sheddases is A Disintegrin And Metalloprotease-17 (ADAM17) whose extracellular region consists of a pro-, a catalytic, a disintegrin-, and a m...

journal_title：Biochemistry

authors： Düsterhöft S,Michalek M,Kordowski F,Oldefest M,Sommer A,Röseler J,Reiss K,Grötzinger J,Lorenzen I

更新日期：2015-09-29 00:00:00

abstract：:Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-prone and lesion-bypass Y-family. In this paper, for the first time, the fidelity of a Y-family polymerase, Dpo4, was determined using pre-steady-state kinetic analysis of the incorporation of a single nucle...

journal_title：Biochemistry

authors： Fiala KA,Suo Z

更新日期：2004-02-24 00:00:00

abstract：:TCDD (dioxin) induces a rapid inflammatory response from 3T3-L1 adipocytes as judged by prominent induction of the mRNA expression of prostaglandin-endperoxide synthase 2 (Cox-2) along with other inflammation markers within 1 h. This action of TCDD is clearly antagonized by cell pretreatment with AACOCF3 (an inhibitor...

journal_title：Biochemistry

authors： Li W,Matsumura F

更新日期：2008-12-30 00:00:00

abstract：:A new bifunctional spin-label (BSL) has been synthesized that can be immobilized on the surface of proteins, allowing measurement of rotational motion of proteins by saturation-transfer electron paramagnetic resonance (STEPR). The spin-label contains a photoactivatable azido moiety, a cleavable disulfide, and a nitrox...

journal_title：Biochemistry

authors： Wilcox MD,Parce JW,Thomas MJ,Lyles DS

更新日期：1990-06-19 00:00:00