Abstract：
:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an important target for the development of anticancer drugs. In addition to ligand-dependent activation and concomitant tyrosine phosphorylation, EGFR stimulation results in the localized generation of H(2)O(2) by NADPH-dependent oxidases. In turn, H(2)O(2) functions as a secondary messenger to regulate intracellular signaling cascades, largely through the modification of specific cysteine residues within redox-sensitive protein targets, including Cys797 in the EGFR active site. In this review, we highlight recent advances in our understanding of the mechanisms that underlie redox regulation of EGFR signaling and how these discoveries may form the basis for the development of new therapeutic strategies for targeting this and other H(2)O(2)-modulated pathways.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Truong TH,Carroll KSdoi
10.1021/bi301441esubject
Has Abstractpub_date
2012-12-18 00:00:00pages
9954-65issue
50eissn
0006-2960issn
1520-4995journal_volume
51pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract：:Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00607a006
更新日期：1978-07-11 00:00:00
abstract：:The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction. In this study, we used electrochemically induced FTIR difference spectroscopy to identify residues involved in redox-couple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0205348
更新日期：2003-05-27 00:00:00
abstract：:The HIV-1 virulence factor Nef interacts with the macrophage Src-family kinase Hck, resulting in constitutive kinase activation that contributes to viral replication and immune escape. Previous chemical library screens identified the diphenylfuranopyrimdine kinase inhibitor DFP-4AB, which selectively inhibits Nef-depe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00875
更新日期：2015-10-20 00:00:00
abstract：:Understanding the evolution of allostery in multidomain enzymes remains an important step in improving our ability to identify and exploit structure-function relationships in allosteric mechanisms. A recent protein similarity network for the DRE-TIM metallolyase superfamily indicated there are two evolutionarily disti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500702u
更新日期：2014-07-29 00:00:00
abstract：:A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00295a023
更新日期：1983-12-20 00:00:00
abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00519a024
更新日期：1981-08-04 00:00:00
abstract：:The production of proteins via recombinant DNA technology often requires the in vitro folding of inclusion bodies, which are protein aggregates. To create a more efficient redox buffer for the in vitro folding of disulfide containing proteins, aromatic thiols were investigated for their ability to increase the folding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034305c
更新日期：2003-10-14 00:00:00
abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991199z
更新日期：2000-02-15 00:00:00
abstract：:To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990600e
更新日期：1999-06-15 00:00:00
abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00285a008
更新日期：1983-08-02 00:00:00
abstract：:Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9724467
更新日期：1998-04-21 00:00:00
abstract：:Clostridium perfringens epsilon toxin belongs to the aerolysin-like family of pore-forming toxins and is one of the most potent bacterial toxins known. The epsilon toxin causes fatal enterotoxemia in sheep, goats, and possibly humans. Evidence indicates that the toxin binds to protein receptors including hepatitis A v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300690a
更新日期：2012-09-25 00:00:00
abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061944p
更新日期：2007-01-09 00:00:00
abstract：:DNA sequences with homology to the major (A + T)-rich mouse satellite component were localized in CsCl gradients by hybridization with a labeled satellite cRNA probe. Although, as expected, most of the hybridization was to DNA in the satellite-rich shoulder, substantial radioactive cRNA hybridized with DNA from denser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00518a025
更新日期：1981-07-21 00:00:00
abstract：:We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00437a002
更新日期：1989-05-30 00:00:00
abstract：:We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00261a031
更新日期：1982-08-31 00:00:00
abstract：:The enzyme RhlI catalyzes the formation of N-butyrylhomoserine lactone from S-adenosylmethionine and N-butyrylacyl carrier protein. N-Butyrylhomoserine lactone serves as a quorum-sensing signal molecule in Pseudomonas aeruginosa, and is implicated in the regulation of many processes involved in bacterial virulence and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048005m
更新日期：2005-03-01 00:00:00
abstract：:The orphan receptor short heterodimer partner (SHP) is a common partner for a great number of nuclear receptors, and it plays an important role in many diverse physiological events. In a previous study, we described SHP as a strong repressor of the androgen receptor (AR). Herein, we addressed the mechanism of action o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047308d
更新日期：2005-04-26 00:00:00
abstract：:A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00240a024
更新日期：1991-07-02 00:00:00
abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010525j
更新日期：2001-09-11 00:00:00
abstract：:It has been shown by genetic analysis that the external and internal invertases from Saccharomyces cerevisiae share a common structural gene [Taussig, R., & Carlson, M. (1983) Nucleic Acids Res. 11, 1943-1954]. However, the only amino acid composition of these two forms of invertase reported to date has revealed exten...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a014
更新日期：1985-10-22 00:00:00
abstract：:The purified PBS2 phage-coded inhibitor of uracil-DNA glycosylase (Ura-DNA glycosylase) from Bacillus subtilis has been tested for its ability to inhibit this enzyme isolated from other prokaryotic and from eukaryotic sources. In addition, the inhibitor has been assayed for its effect on DNA glycosylases specific for ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00524a027
更新日期：1981-10-13 00:00:00
abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00028a022
更新日期：1995-07-18 00:00:00
abstract：:Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a024
更新日期：1985-10-22 00:00:00
abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a023
更新日期：1995-05-30 00:00:00
abstract：:The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A reso...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049218e
更新日期：2004-08-31 00:00:00
abstract：:Adenosine cyclic 3',5'-phosphate receptor protein (CRP or CAP) is a regulatory protein involved in the transcription of several operons in Escherichia coli. cAMP-independent, nonspecific complexes of CRP and DNA were investigated by photochemical cross-linking of the protein to nonspecific DNA, whose thymines are subs...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00058a010
更新日期：1993-02-23 00:00:00
abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a069
更新日期：1986-11-18 00:00:00
abstract：:For the study of the protein--protein and protein--nucleic acid interactions in the assembly of virus particles, laser Raman spectra have been obtained in H2O and D2O solutions and as a function of temperature for the following Salmonella phage P22 components: mature phage particles, isolated mature phage DNA, mature ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00259a023
更新日期：1982-08-03 00:00:00
abstract：:We have measured T1 and T2 of protein and water protons in hemoglobin solutions using broad-line pulse techniques; selective excitation and detection methods enabled the intrinsic protein and water relaxation rates, as well as the spin-transfer rate between them, to be obtained at 5, 10, and 20 MHz. Water and protein ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00335a004
更新日期：1985-07-02 00:00:00
