Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.

abstract：:The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...

journal_title：Biochemistry

authors： Venkaiah B,Viswanathan P,Habib S,Hasnain SE

更新日期：2004-06-29 00:00:00

abstract：:A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...

journal_title：Biochemistry

authors： Sia SK,Kim PS

更新日期：2001-07-31 00:00:00

abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...

journal_title：Biochemistry

authors： Rogl H,Schödel R,Lokstein H,Kühlbrandt W,Schubert A

更新日期：2002-02-19 00:00:00

abstract：:Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...

journal_title：Biochemistry

authors： Grzyska PK,Ryle MJ,Monterosso GR,Liu J,Ballou DP,Hausinger RP

更新日期：2005-03-15 00:00:00

abstract：:The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...

journal_title：Biochemistry

authors： Stoddard BL,Dean A,Koshland DE Jr

更新日期：1993-09-14 00:00:00

abstract：:The potency of cholesterol to affect the acyl chain order and dynamics of cardiolipin membranes in the liquid-crystalline state was monitored by steady-state and time-resolved fluorescence anisotropy as well as excited-state lifetime measurements with cis- and trans-parinaric acids as probes. Up to a cholesterol mole ...

journal_title：Biochemistry

authors： Gallay J,Vincent M

更新日期：1986-05-06 00:00:00

abstract：:The mammalian HIRA/UBN1/CABIN1/ASF1a (HUCA) histone chaperone complex deposits the histone H3 variant H3.3 into chromatin and is linked to gene activation, repression, and chromatin assembly in diverse cell contexts. We recently reported that a short N-terminal fragment of UBN1 containing amino acids 1-175 is necessar...

journal_title：Biochemistry

authors： Tang Y,Puri A,Ricketts MD,Rai TS,Hoffmann J,Hoi E,Adams PD,Schultz DC,Marmorstein R

更新日期：2012-03-27 00:00:00

abstract：:High-resolution nitrite soaked oxidized and reduced crystal structures of two active site mutants, D98N and H255N, of nitrite reductase (NIR) from Alcaligenes faecalis S-6 were determined to better than 2.0 A resolution. In the oxidized D98N nitrite-soaked structures, nitrite is coordinated to the type II copper via i...

journal_title：Biochemistry

authors： Boulanger MJ,Murphy ME

更新日期：2001-08-07 00:00:00

abstract：:By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Sp...

journal_title：Biochemistry

authors： Nagaoka M,Sugiura Y

更新日期：1996-07-02 00:00:00

abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...

journal_title：Biochemistry

authors： Krucinska J,Falcone E,Erlandsen H,Hazeen A,Lombardo MN,Estrada A,Robinson VL,Anderson AC,Wright DL

更新日期：2019-03-05 00:00:00

abstract：:To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM-Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to N...

journal_title：Biochemistry

authors： Himes RD,Smolin N,Kukol A,Bossuyt J,Bers DM,Robia SL

更新日期：2016-11-08 00:00:00

abstract：:Putative physiological partners of the [2Fe-2S] ferredoxin from Clostridium pasteurianum have been searched by running crude soluble extracts of this bacterium through an affinity column to which the [2Fe-2S] ferredoxin had been covalently bound. Subsequent washing of the column with buffers of increasing ionic streng...

journal_title：Biochemistry

authors： Golinelli MP,Gagnon J,Meyer J

更新日期：1997-09-30 00:00:00

abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...

journal_title：Biochemistry

authors： Jin L,Yang J,Carey J

更新日期：1993-07-20 00:00:00

abstract：:The relative activities of 313 mutants of the gene V protein of bacteriophage f1, assayed in vivo, have been used to evaluate two approaches to predicting the effects of single amino acid substitutions on the function of a protein. First, we tested methods that only depend on the properties of the wild-type and substi...

journal_title：Biochemistry

authors： Zabin HB,Horvath MP,Terwilliger TC

更新日期：1991-06-25 00:00:00

abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...

journal_title：Biochemistry

authors： Ziehler WA,Day JJ,Fierke CA,Engelke DR

更新日期：2000-08-15 00:00:00

abstract：:Vibrational circular dichroism (VCD) and Fourier transform IR (FTIR) were measured for a series of short alanine-based peptides having the general formula Ac-(AAKAA)n-GY-NH2 (n = 1-4) from 5 to 50 degrees C in D2O and at room temperature in both TFE and H2O. In both of these latter solvents, the dominant structural fo...

journal_title：Biochemistry

authors： Yoder G,Pancoska P,Keiderling TA

更新日期：1997-12-09 00:00:00

abstract：:Insulin and insulin-like growth factor 1 (IGF-1) share high sequence homology, but their folding behaviors are significantly different: insulin folds into one unique thermodynamically controlled structure, while IGF-1 folds into two thermodynamically controlled disulfide isomers. However, the origin of their different...

journal_title：Biochemistry

authors： Wang S,Guo ZY,Shen L,Zhang YJ,Feng YM

更新日期：2003-08-19 00:00:00

abstract：:The ovalbumin (Ov) gene contains a number of regulatory elements that control its transcriptional activity and restrict expression to avian oviduct. One major regulatory region, the steroid-dependent regulatory element (SDRE), is required for induction by estrogen and corticosterone. Another region, the negative regul...

journal_title：Biochemistry

authors： Park HM,Haecker SE,Hagen SG,Sanders MM

更新日期：2000-07-25 00:00:00

abstract：:We have employed molecular dynamics (MD) simulation to investigate, with atomic details, the structural dynamics and energetics of three major ATPase states (ADP, APO, and ATP state) of a human kinesin-1 monomer in complex with a tubulin dimer. Starting from a recently solved crystal structure of ATP-like kinesin-tubu...

journal_title：Biochemistry

authors： Chakraborty S,Zheng W

更新日期：2015-01-27 00:00:00

abstract：:Oxidative damage by transition metals bound to proteins may be an important pathogenic mechanism. Ceruloplasmin (Cp) is a Cu-containing plasma protein thought to be involved in oxidative modification of lipoproteins. We have previously shown that Cp increased cell-mediated low-density lipoprotein (LDL) oxidation by a ...

journal_title：Biochemistry

authors： Mukhopadhyay CK,Fox PL

更新日期：1998-10-06 00:00:00

abstract：:Calculations of the electrostatic potentials were made around yeast elongator phenylalanine, aspartate tRNAs, and yeast initiator methionine tRNA in aqueous solution at physiological ionic strength. The calculations were carried out with a finite difference algorithm for solving the nonlinear Poisson-Boltzmann equatio...

journal_title：Biochemistry

authors： Sharp KA,Honig B,Harvey SC

更新日期：1990-01-16 00:00:00

abstract：:Ultraviolet (uv)-light-mediated formation of protein-DNA adducts in Chinese hamster cell chromatin was investigated in an attempt to compare chromatin alterations induced in vitro with those observed in vivo. Three independent methods of analysis indicated stable protein-DNA associations: (1) a membrane filter assay w...

journal_title：Biochemistry

authors： Strniste GF,Rall SC

更新日期：1976-04-20 00:00:00

abstract：:A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...

journal_title：Biochemistry

authors： Du D,Ju H,Zhang X,Chen J,Cai J,Chen H

更新日期：2005-08-30 00:00:00

abstract：:The membrane topology model of the Na(+)-citrate transporter CitS of Klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. A split version of CitS was constructed to study domain interactions and proximity relationships of...

journal_title：Biochemistry

authors： Dobrowolski A,Fusetti F,Lolkema JS

更新日期：2010-06-01 00:00:00

abstract：:DNA topoisomerase II is believed to be the enzyme that produces the protein-associated DNA strand breaks observed in mammalian cell nuclei treated with various intercalating agents. Two intercalators--4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA, amsacrine) and 2-methyl-9-hydroxyellipticinium (2-Me-9-OH-E+)-...

journal_title：Biochemistry

authors： Pommier Y,Minford JK,Schwartz RE,Zwelling LA,Kohn KW

更新日期：1985-11-05 00:00:00

abstract：:The glycolytic enzyme fructose-1,6-bisphosphate aldolase (FBPA) catalyzes the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Catalysis of Schiff base forming class I FBPA relies on a number of intermediates covalently bound to the catalytic lysine. Using ...

journal_title：Biochemistry

authors： Lorentzen E,Siebers B,Hensel R,Pohl E

更新日期：2005-03-22 00:00:00

abstract：:The occurrence of 8-oxo-7,8-dihydroguanine (OG) in the genome, as one of the major DNA oxidative damages, has been implicated in an array of biological processes, ranging from mutagenesis to transcriptional regulation. Genome-wide mapping of oxidative damages could shed light on the underlying cellular mechanism. In t...

journal_title：Biochemistry

authors： Fang Y,Zou P

更新日期：2020-01-14 00:00:00

abstract：:GTPase-activating proteins (GAPs) enhance the intrinsic GTPase activity of small G proteins, such as Ras and Rho, by contributing a catalytic arginine to the active site. An intramolecular arginine plays a similar role in heterotrimeric G proteins. Aluminum fluoride activates the GDP form of heterotrimeric G proteins,...

journal_title：Biochemistry

authors： Graham DL,Eccleston JF,Chung CW,Lowe PN

更新日期：1999-11-09 00:00:00

abstract：:Protein C inhibitor (PCI) is a versatile serine protease inhibitor with both pro- and anticoagulant and other properties. Interactions of certain ligands with PCI, including heparin, affect its specificity for proteases. In this study, heparin was found to enhance PCI inhibition of factor Xa up to 42-fold in the prese...

journal_title：Biochemistry

authors： Sun W,Eriksson AS,Schedin-Weiss S

更新日期：2009-02-10 00:00:00

abstract：:Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent on the presence of negatively charged lipids. By using a phospholipid with an acyl chain linked photoactivatable group,...

journal_title：Biochemistry

authors： van Voorst F,van der Does C,Brunner J,Driessen AJ,de Kruijff B

更新日期：1998-09-01 00:00:00