当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Distinct phosphate backbone contacts revealed by some mutant peptides of zinc finger protein Spl: effect of protein-induced bending on DNA recognition.

Distinct phosphate backbone contacts revealed by some mutant peptides of zinc finger protein Spl: effect of protein-induced bending on DNA recognition.

Abstract：

:By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Spl(R565S) and Spl(K595S) mutants demonstrate that arginine at 565 position and lysine at 595 position interact with the phosphate between G(3) and G(4) and with the phosphate between G(9) and G(10) in GC-box DNA, respectively. On the basis of the experimental results for Spl(K535G), Sp1(537-623), and Sp1(530-623), lysine and glutamine at 535 and 536 positions have been clarified to be in contacts with phosphate between G(7) and G(8) and with phosphate outside GC-box, respectively. In particular, glutamine at the N-terminal side of zinc finger 1 is a key amino acid residue to induce DNA bending and also participates in total base specificity of Sp1. The present study strongly indicates that (1) each zinc finger is not independent for the DNA interaction with Sp1 and (2) DNA base recognition of the zinc finger protein is influenced by local conformational change of DNA induced by the protein binding.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Nagaoka M,Sugiura Y

doi

10.1021/bi952530r

subject

Has Abstract

pub_date

1996-07-02 00:00:00

pages

8761-8

issue

26

eissn

0006-2960

issn

1520-4995

pii

bi952530r

journal_volume

35

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Substrate specificity, metal binding properties, and spectroscopic characterization of the DapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae.

    abstract：:The catalytic and structural properties of divalent metal ion cofactor binding sites in the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae were investigated. Co(II)-substituted DapE enzyme was 25% more active than the Zn(II)-loaded form of the enzyme. Interestingly, Mn...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034845+

    authors： Bienvenue DL,Gilner DM,Davis RS,Bennett B,Holz RC

    更新日期：2003-09-16 00:00:00

  • The three-dimensional solution structure of the SH2 domain from p55blk kinase.

    abstract：:Signal transduction in B cells is mediated, in part, by the interaction of the cytoplasmic components of the antigen receptor complex and various members of the src family tyrosine kinases. Key to this process appears to be the interaction of the tyrosine kinase SH2 domains with the tyrosine-phosphorylated cytoplasmic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960157x

    authors： Metzler WJ,Leiting B,Pryor K,Mueller L,Farmer BT 2nd

    更新日期：1996-05-21 00:00:00

  • Fate of the (2Fe-2S)(2+) cluster of Escherichia coli biotin synthase during reaction: a Mössbauer characterization.

    abstract：:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detai...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034426c

    authors： Tse Sum Bui B,Benda R,Schünemann V,Florentin D,Trautwein AX,Marquet A

    更新日期：2003-07-29 00:00:00

  • Activation of GlcNAc-P-P-dolichol synthesis by mannosylphosphoryldolichol is stereospecific and requires a saturated alpha-isoprene unit.

    abstract：:Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00200a036

    authors： Kean EL,Rush JS,Waechter CJ

    更新日期：1994-08-30 00:00:00

  • Ethanol enhances agonist-induced fast desensitization in nicotinic acetylcholine receptors.

    abstract：:The reversible decline of the nicotinic acetylcholine receptor's response to acetylcholine during prolonged exposure to acetylcholine is known as desensitization. Here, we studied ethanol's modulation of fast agonist-induced desensitization of the nicotinic acetylcholine receptor in postsynaptic membrane vesicles from...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00197a009

    authors： Wu G,Miller KW

    更新日期：1994-08-09 00:00:00

  • Topography of the Dictyostelium discoideum plasma membrane: analysis of membrane asymmetry and intermolecular disulfide bonds.

    abstract：:Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00389a041

    authors： Shiozawa JA,Jelenska MM,Jacobson BS

    更新日期：1987-07-28 00:00:00

  • Amino-terminal domain of TIF2 is involved in competing for corepressor binding to glucocorticoid and progesterone receptors.

    abstract：:Both agonist- and antagonist-bound glucocorticoid receptors (GRs) and progesterone receptors (PRs) regulate gene transcription with the assistance of corepressors (NCoR and SMRT) and coactivators (TIF2/GRIP1, SRC1, and AIB1). Receptor binding of these cofactors is competitive and is considered to involve interactions ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7004575

    authors： Wang D,Wang Q,Awasthi S,Simons SS Jr

    更新日期：2007-07-10 00:00:00

  • Incorporation of an Unnatural Amino Acid as a Domain-Specific Fluorescence Probe in a Two-Domain Protein.

    abstract：:The biophysical analysis of multidomain proteins often is difficult because of overlapping signals from the individual domains. Previously, the fluorescent unnatural amino acid p-cyanophenylalanine has been used to study the folding of small single-domain proteins. Here we extend its use to a two-domain protein to sel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00898

    authors： Ries LK,Schmid FX,Schmidpeter PA

    更新日期：2016-12-13 00:00:00

  • Evidence for retro-translocation of pokeweed antiviral protein from endoplasmic reticulum into cytosol and separation of its activity on ribosomes from its activity on capped RNA.

    abstract：:Pokeweed antiviral protein (PAP) is a single-chain ribosome inactivating protein (RIP) that binds to ribosomes and depurinates the highly conserved alpha-sarcin/ricin loop (SRL) of the large subunit rRNA. Catalytic depurination of a specific adenine has been proposed to result in translation arrest and cytotoxicity. H...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048188c

    authors： Parikh BA,Baykal U,Di R,Tumer NE

    更新日期：2005-02-22 00:00:00

  • Thermomechanical and transition properties of dimyristoylphosphatidylcholine/cholesterol bilayers.

    abstract：:Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical properties--elastic moduli and level of tension at membrane rupture--w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00413a013

    authors： Needham D,McIntosh TJ,Evans E

    更新日期：1988-06-28 00:00:00

  • Role of lys100 in human dihydroorotate dehydrogenase: mutagenesis studies and chemical rescue by external amines.

    abstract：:Chemical modification, mutagenesis, chemical rescue, and isotope effect studies are used to identify and probe the roles of several conserved amino acid groups in catalysis by human dihydroorotate dehydrogenase. Time- and pH-dependent inactivation of human dihydroorotate dehydrogenase by trinitrobenzenesulfonate impli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000630d

    authors： Jiang W,Locke G,Harpel MR,Copeland RA,Marcinkeviciene J

    更新日期：2000-07-11 00:00:00

  • Compartmentation of glucose and fructose 1,6-bisphosphate metabolism in vascular smooth muscle.

    abstract：:We examined the metabolism of exogenously added 13C-labeled fructose 1,6-bisphosphate (either labeled at the first and sixth carbons or labeled at the first carbon only) and of [2-13C]glucose in well-oxygenated and well-superfused hog carotid artery segments. Exogenously added fructose 1,6-bisphosphate was utilized by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00004a027

    authors： Hardin CD,Roberts TM

    更新日期：1995-01-31 00:00:00

  • Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin to probe the sodium channel of excitable membranes.

    abstract：:Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin (TTX) have been synthesized. N-Methylanthraniloylglycine hydrazide, anthraniloyl hydrazide, and 2-azidoanthraniloylglycine hydrazide were coupled to the carbonyl at C6 of oxidized tetrodotoxin to form stable fluorescent hydrazones. The C6 ketone ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00517a025

    authors： Angelides KJ

    更新日期：1981-07-07 00:00:00

  • Quantification of quaternary structure stability in aggregation-prone proteins under physiological conditions: the transthyretin case.

    abstract：:The quaternary structure stability of proteins is typically studied under conditions that accelerate their aggregation/unfolding processes on convenient laboratory time scales. Such conditions include high temperature or pressure, chaotrope-mediated unfolding, or low or high pH. These approaches have the limitation of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500739q

    authors： Robinson LZ,Reixach N

    更新日期：2014-10-21 00:00:00

  • Subunit structure of human erythrocyte glycophorin A.

    abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00650a028

    authors： Furthmayr H,Marchesi VT

    更新日期：1976-03-09 00:00:00

  • Dissection of CDK4-binding and transactivation activities of p34(SEI-1) and comparison between functions of p34(SEI-1) and p16(INK4A).

    abstract：:Recent studies showed that p34(SEI-1), also known as TRIP-Br1 or SEI-1, plays a dual role in the regulation of cell-cycle progression. It exhibits the transactivation activity and regulates a number of genes required for G1/S transition, while it also binds and activates cyclin-dependent kinase 4 (CDK4) independent of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0504658

    authors： Li J,Muscarella P,Joo SH,Knobloch TJ,Melvin WS,Weghorst CM,Tsai MD

    更新日期：2005-10-11 00:00:00

  • A conserved region in alpha-macroglobulins participates in binding to the mammalian alpha-macroglobulin receptor.

    abstract：:Efforts to characterize the receptor recognition domain of alpha-macroglobulins have primarily focused on human alpha 2-macroglobulin (alpha 2M). In the present work, the structure and function of the alpha-macroglobulin receptor recognition site were investigated by amino acid sequence analysis, plasma clearance, and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00429a069

    authors： Enghild JJ,Thøgersen IB,Roche PA,Pizzo SV

    更新日期：1989-02-07 00:00:00

  • Insulin allosteric behavior: detection, identification, and quantification of allosteric states via 19F NMR.

    abstract：:The insulin hexamer is an allosteric protein widely used in formulations for the treatment of diabetes. The hexamer exhibits positive and negative cooperativity and apparent half-site binding activity, reflecting the interconversion of three allosteric states, designated as T6, T3R3, and R6. The hexamer contains two s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050392s

    authors： Bonaccio M,Ghaderi N,Borchardt D,Dunn MF

    更新日期：2005-05-31 00:00:00

  • Endogenous antithrombin associated with microvascular endothelium. Quantitative analysis in perfused rat hearts.

    abstract：:A recirculating Langendorff heart preparation is used to characterize the endogenous antithrombin associated reversibly with murine vascular endothelium. Rat hearts are perfused clear of blood and then recirculated with a physiological salt solution. Addition of heparin educes antithrombin activity continuously into t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00169a024

    authors： Felsch JS,Owen WG

    更新日期：1994-01-25 00:00:00

  • Vibrationally enhanced hydrogen tunneling in the Escherichia coli thymidylate synthase catalyzed reaction.

    abstract：:The enzyme thymidylate synthase (TS) catalyzes a complex reaction that involves forming and breaking at least six covalent bonds. The physical nature of the hydride transfer step in this complex reaction cascade has been studied by means of isotope effects and their temperature dependence. Competitive kinetic isotope ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi036124g

    authors： Agrawal N,Hong B,Mihai C,Kohen A

    更新日期：2004-02-24 00:00:00

  • Identification of N(G)-methylarginine residues in human heterogeneous RNP protein A1: Phe/Gly-Gly-Gly-Arg-Gly-Gly-Gly/Phe is a preferred recognition motif.

    abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9625509

    authors： Kim S,Merrill BM,Rajpurohit R,Kumar A,Stone KL,Papov VV,Schneiders JM,Szer W,Wilson SH,Paik WK,Williams KR

    更新日期：1997-04-29 00:00:00

  • Structural studies of two mutants of amicyanin from Paracoccus denitrificans that stabilize the reduced state of the copper.

    abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049634z

    authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

    更新日期：2004-07-27 00:00:00

  • Fluorescence correlation spectroscopy detects galanin receptor diversity on insulinoma cells.

    abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010514q

    authors： Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

    更新日期：2001-09-11 00:00:00

  • Cholesterol distribution in renal epithelial cells LLC-PK1 as determined by cholesterol oxidase: evidence that glutaraldehyde fixation masks plasma membrane cholesterol pools.

    abstract：:Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00174a046

    authors： el Yandouzi EH,Zlatkine P,Moll G,Le Grimellec C

    更新日期：1994-03-01 00:00:00

  • Inactivation of factor VIII by factor IXa.

    abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00125a022

    authors： O'Brien DP,Johnson D,Byfield P,Tuddenham EG

    更新日期：1992-03-17 00:00:00

  • Product Release Mechanism Associated with Structural Changes in Monomeric l-Threonine 3-Dehydrogenase.

    abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00832

    authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

    更新日期：2017-10-31 00:00:00

  • Transient aggregation and stable dimerization induced by introducing an Alzheimer sequence into a water-soluble protein.

    abstract：:Transient contacts between denatured polypeptide chains are likely to play an important part in the initial stages of protein aggregation and fibrillation. To analyze the nature of such contacts, we have carried out a protein engineering study of the 102-residue protein U1A, which aggregates transiently in the wild-ty...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048509k

    authors： Otzen DE,Miron S,Akke M,Oliveberg M

    更新日期：2004-10-19 00:00:00

  • Purification, characterization, and investigation of the mechanism of aminoglycoside 3'-phosphotransferase type Ia.

    abstract：:Aminoglycoside 3'-phosphotransferases [APH(3')s] are the most common cause of bacterial high-level resistance to aminoglycoside antibiotics in clinical isolates. A one-step affinity chromatography was used to purify APH(3') type Ia. The kinetic parameters for turnover of seven aminoglycosides and the corresponding min...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00039a026

    authors： Siregar JJ,Miroshnikov K,Mobashery S

    更新日期：1995-10-03 00:00:00

  • Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial species.

    abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00223a035

    authors： Oller AR,Vanden Broek W,Conrad M,Topal MD

    更新日期：1991-03-05 00:00:00

  • Effect of the inhibitor-resistant M69V substitution on the structures and populations of trans-enamine beta-lactamase intermediates.

    abstract：:The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060990m

    authors： Totir MA,Padayatti PS,Helfand MS,Carey MP,Bonomo RA,Carey PR,van den Akker F

    更新日期：2006-10-03 00:00:00