Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial species.

abstract：:The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this reaction by the EcoRV methyltransferase. However, in vitro, the EcoRV n...

journal_title：Biochemistry

authors： Taylor JD,Goodall AJ,Vermote CL,Halford SE

更新日期：1990-12-04 00:00:00

abstract：:Substitution of cysteine for proline-39 in Escherichia coli dihydrofolate reductase by oligonucleotide-directed mutagenesis positions the new cysteine adjacent to already existing cysteine-85. When the mutant protein is expressed in the E. coli cytosol, the cysteine sulfur atoms are found, by X-ray crystallographic an...

journal_title：Biochemistry

authors： Villafranca JE,Howell EE,Oatley SJ,Xuong NH,Kraut J

更新日期：1987-04-21 00:00:00

abstract：:To understand the details of rate limitation of turnover of the photosynthetic reaction center, photooxidation of horse heart cytochrome c by reaction center from Rhodobacter spheroides in detergent dispersion has been examined by intense continuous illumination under a wide variety of conditions of cytochrome concent...

journal_title：Biochemistry

authors： Gerencsér L,Laczkó G,Maróti P

更新日期：1999-12-21 00:00:00

abstract：:Two site-directed mutant enzymes of the class A beta-lactamase from Staphylococcus aureus PC1 were produced with the goal of blocking the site that in the native enzyme is occupied by the proposed hydrolytic water molecule. The crystal structures of these two mutant enzymes, N170Q and N170M, have been determined and r...

journal_title：Biochemistry

authors： Zawadzke LE,Chen CC,Banerjee S,Li Z,Wäsch S,Kapadia G,Moult J,Herzberg O

更新日期：1996-12-24 00:00:00

abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...

journal_title：Biochemistry

authors： van Ingen H,Lasonder E,Jansen JF,Kaan AM,Spronk CA,Stunnenberg HG,Vuister GW

更新日期：2004-01-13 00:00:00

abstract：:The role of the tryptophan residues in the substrate-binding and catalytic mechanism of an enzymatically active C-terminal fragment of Pseudomonas aeruginosa exotoxin A was studied by individually or jointly replacing these residues with phenylalanine. Substitution of W-466 decreased the ADP-ribosyltransferase and NAD...

journal_title：Biochemistry

authors： Beattie BK,Prentice GA,Merrill AR

更新日期：1996-12-03 00:00:00

abstract：:Human platelet glycoproteins Ib alpha, Ib beta, V, and IX comprise an interrelated set of molecules (the Ib-V-IX system) that together form a surface adhesion receptor for the ligand, von Willebrand factor. To complete the primary structural characterization of the genes involved in this system, we have analyzed cosmi...

journal_title：Biochemistry

authors： Yagi M,Edelhoff S,Disteche CM,Roth GJ

更新日期：1995-12-12 00:00:00

abstract：:Our laboratory has been studying the transcriptional regulation of the nuclear gene (ATPA) that encodes the alpha-subunit of the mammalian mitochondrial F1F0 ATP synthase complex. We have previously determined that the regulatory factor, upstream stimulatory factor 2 (USF2), can stimulate transcription of the ATPA gen...

journal_title：Biochemistry

authors： Jordan EM,Worley T,Breen GA

更新日期：2003-03-11 00:00:00

abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...

journal_title：Biochemistry

authors： Sancho J,Serrano L,Fersht AR

更新日期：1992-03-03 00:00:00

abstract：:The Hsp70-class molecular chaperone HscA interacts specifically with a conserved (99)LPPVK(103) motif of the iron-sulfur cluster scaffold protein IscU. We used a cellulose-bound peptide array to perform single-site saturation substitution of peptide residues corresponding to Glu(98)-Ile(104) of IscU to determine posit...

journal_title：Biochemistry

authors： Tapley TL,Cupp-Vickery JR,Vickery LE

更新日期：2006-07-04 00:00:00

abstract：:We used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran Psi-coordinate of a mainly poly-Ala peptide, AP with an AAAAA(AAARA)(3)A sequence. 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-heli...

journal_title：Biochemistry

authors： Xiong K,Asher SA

更新日期：2010-04-20 00:00:00

abstract：:The cytokine hormone interleukin-2 (IL-2) contains a highly adaptive region that binds small, druglike molecules. The binding properties of this adaptive region have been explored using a "tethering" method that relies on the formation of a disulfide bond between the protein and small-molecule ligands. Using tethering...

journal_title：Biochemistry

authors： Hyde J,Braisted AC,Randal M,Arkin MR

更新日期：2003-06-03 00:00:00

abstract：:The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. The assoc...

journal_title：Biochemistry

authors： Dell VA,Miller DL,Johnson AE

更新日期：1990-02-20 00:00:00

abstract：:The cellular site of formation, Galpha-coupling preference, and agonist regulation of mu-delta opioid receptor (OR) heterooligomers were studied. Bioluminescence resonance energy transfer (BRET) showed that mu-deltaOR heterooligomers, composed of preformed mu and delta homooligomers, interacted constitutively in the e...

journal_title：Biochemistry

authors： Hasbi A,Nguyen T,Fan T,Cheng R,Rashid A,Alijaniaram M,Rasenick MM,O'Dowd BF,George SR

更新日期：2007-11-13 00:00:00

abstract：:Natriuretic peptide receptor A (NPRA) is a noncovalent homodimeric receptor, composed of an extracellular domain (ECD) with a ligand-binding site, a single transmembrane domain (TM), and an intracellular domain (ICD) exhibiting guanylyl cyclase activity. NPRA activation by atrial natriuretic peptide (ANP) leads to cGM...

journal_title：Biochemistry

authors： Parat M,Blanchet J,De Léan A

更新日期：2010-06-08 00:00:00

abstract：:Mercury ions can inhibit Candida albicans phosphomannose isomerase (PMI) by two different processes at sub-micromolar concentrations. Kinetic studies show that mercury ions are in rapid equilibrium with the enzyme and cause a clear partial noncompetitive inhibition when mannose 6-phosphate is used as the substrate. Th...

journal_title：Biochemistry

authors： Wells TN,Payton MA,Proudfoot AE

更新日期：1994-06-21 00:00:00

abstract：:S100 beta is a calcium-binding protein, which regulates the activities of several enzymes and inhibits the phosphorylation of a variety of protein kinase C substrates in a calcium-dependent manner. The protein was recently found to activate a retinal membrane guanylate cyclase, and in this paper, we report that it inh...

journal_title：Biochemistry

authors： Pozdnyakov N,Margulis A,Sitaramayya A

更新日期：1998-07-28 00:00:00

abstract：:The stability and folding kinetics of wild-type and a mutant staphylococcal nuclease (SNase) at neutral pH are significantly perturbed by the presence of moderate to high concentrations of salts. Very substantial increases in stability toward thermal and urea denaturation were observed; for example, 0.4 M sodium sulfa...

journal_title：Biochemistry

authors： Nishimura C,Uversky VN,Fink AL

更新日期：2001-02-20 00:00:00

abstract：:A conformational transition is described for the polypeptide, gramicidin A, in which a dimer that forms a left-handed intertwined antiparallel helix is converted to a single-stranded amino terminus to amino terminus right-handed helix. The starting structure is determined here by solution NMR methods while reference i...

journal_title：Biochemistry

authors： Zhang Z,Pascal SM,Cross TA

更新日期：1992-09-22 00:00:00

abstract：:Fom3, the antepenultimate enzyme in the fosfomycin biosynthetic pathway in Streptomyces spp., is a class B cobalamin-dependent radical SAM methyltransferase that catalyzes methylation of (5'-cytidylyl)-2-hydroxyethylphosphonate (2-HEP-CMP) to form (5'-cytidylyl)-2-hydroxypropylphosphonate (2-HPP-CMP). Previously, the ...

journal_title：Biochemistry

authors： McLaughlin MI,van der Donk WA

更新日期：2018-08-21 00:00:00

abstract：:Thioacetamide, a hepatocarcinogen and an inhibitor of heme synthesis, blocks the phenobarbitone-mediated increase in the transcription of cytochrome P-450b+e messenger RNA in rat liver. This property is also shared by CoCl2 and 3-amino-1,2,4-triazole, two other inhibitors of heme synthesis. Thus, it appears feasible t...

journal_title：Biochemistry

authors： Satyabhama S,Seelan RS,Padmanaban G

更新日期：1986-08-12 00:00:00

abstract：:The flavoenzyme tryptophan 2-monooxygenase catalyzes the oxidation of tryptophan to indoleacetamide, carbon dioxide, and water. The enzyme is a homologue of l-amino acid oxidase. In the structure of l-amino acid oxidase complexed with aminobenzoate, Tyr372 hydrogen bonds with the carboxylate of the inhibitor in the ac...

journal_title：Biochemistry

authors： Sobrado P,Fitzpatrick PF

更新日期：2003-12-02 00:00:00

abstract：:We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...

journal_title：Biochemistry

authors： Bjornson KP,Amaratunga M,Moore KJ,Lohman TM

更新日期：1994-11-29 00:00:00

abstract：:Spectroscopic and kinetic methods have been used to explore the roles of divalent metal ions in the enolase-catalyzed dehydration of 2-phosphoglycerate (2-PGA). Enolase requires 2 equiv of metal ion per active site for maximal activity. Previous crystallographic studies [Larsen, T. M., Wedekind, J. E., Rayment, I., an...

journal_title：Biochemistry

authors： Poyner RR,Cleland WW,Reed GH

更新日期：2001-07-10 00:00:00

abstract：:(E)-4-(4-Chlorophenyl)-2-oxo-3-butenoic acid (CPB) was found to irreversibly inactivate brewers' yeast pyruvate decarboxylase (PDC, EC 4.1.1.1) in a biphasic, sigmoidal manner, as is found for the kinetic behavior of substrate. An expression was derived for two-site irreversible inhibition of allosteric enzymes, and t...

journal_title：Biochemistry

authors： Kuo DJ,Jordan F

更新日期：1983-08-02 00:00:00

abstract：:The protein tyrosine kinase p72syk readily phosphorylates hematopoietic linkage cell-specific protein p50/HS1 with high stoichiometry (up to 4 mol of Pi/mol of protein) and favorable kinetic constants (Km 77 nM, kcat 0.37 s-1), at sites that display the motif that is specifically recognized by the HS2 domains of Src t...

journal_title：Biochemistry

authors： Ruzzene M,Brunati AM,Marin O,Donella-Deana A,Pinna LA

更新日期：1996-04-23 00:00:00

abstract：:Endonuclease VIII (Nei) is one of three enzymes in Escherichia coli that are involved in base-excision repair of oxidative damage to DNA. We investigated the substrate specificity and excision kinetics of this DNA glycosylase for bases in DNA that have been damaged by free radicals. Two different DNA substrates were p...

journal_title：Biochemistry

authors： Dizdaroglu M,Burgess SM,Jaruga P,Hazra TK,Rodriguez H,Lloyd RS

更新日期：2001-10-09 00:00:00

abstract：:Fluorescence resonance energy transfer (FRET) has been used to investigate the conformation of the single stranded region for a series of fluorescent DNA template-primers bound to the Klenow fragment (KF) of Escherichia coli DNA polymerase I. Fluorescent derivatives of template-primer DNA, modified with tetramethylrho...

journal_title：Biochemistry

authors： Furey WS,Joyce CM,Osborne MA,Klenerman D,Peliska JA,Balasubramanian S

更新日期：1998-03-03 00:00:00

abstract：:Stopped-flow measurements have been employed to study the kinetics of the conformational changes in TetR (B) induced by tetracycline binding with and without Mg(2+) ions. Result of stopped-flow fluorometry measurements at pH 8.0 indicate conformational changes in the helix-turn-helix motif in the N-terminal domain and...

journal_title：Biochemistry

authors： Kedracka-Krok S,Gorecki A,Bonarek P,Wasylewski Z

更新日期：2005-01-25 00:00:00

abstract：:Indolicidin is an antimicrobial cationic peptide with broad-spectrum activity isolated from bovine neutrophils. An indolicidin analogue CP-11, ILKKWPWWPWRRK-NH(2), with improved activity against Gram-negative bacteria had increased positive charge and amphipathicity while maintaining the short length of the parent mol...

journal_title：Biochemistry

authors： Rozek A,Powers JP,Friedrich CL,Hancock RE

更新日期：2003-12-09 00:00:00