Abstract:
:Natriuretic peptide receptor A (NPRA) is a noncovalent homodimeric receptor, composed of an extracellular domain (ECD) with a ligand-binding site, a single transmembrane domain (TM), and an intracellular domain (ICD) exhibiting guanylyl cyclase activity. NPRA activation by atrial natriuretic peptide (ANP) leads to cGMP production, which plays important roles in cardiovascular homeostasis. Initial studies have shown that activation of NPRA involves a conformational change in the juxtamembrane domain (JM). However, crystallographic study of the soluble ECD of NPRA has failed to document JM structure, and the conformational change involved in transmembrane signal transduction is still unknown. To analyze this conformational change, we first sequentially substituted nine amino acids of the JM with a cysteine residue. By studying the mutant's capacity to form ANP-induced or constitutive covalent disulfide dimers, we evaluated the relative proximity of JM residues, before and after NPRA activation. These results obtained with the full-length receptor demonstrate a high proximity of specific JM residues and are in disagreement with crystallography data. We also tested the hypothesis that signal transduction involves a TM rotation mechanism leading to ICD activation. By introducing one to five alanine residues into the TM alpha-helix, we show that a TM rotation of 40 degrees leads to constitutive NPRA activation. We finally studied the role of the TM in NPRA dimerization. By using the ToxR system, we demonstrate that the last JM residues are required to stabilize the TM dimer. Using these experimental data, we generated a new molecular model illustrating the active conformation of NPRA, where the JM and TM are depicted.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Parat M,Blanchet J,De Léan Adoi
10.1021/bi901711wsubject
Has Abstractpub_date
2010-06-08 00:00:00pages
4601-10issue
22eissn
0006-2960issn
1520-4995journal_volume
49pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Prion diseases, also known as the transmissible spongiform encephalopathies (TSEs), are a group of fatal neurodegenerative disorders that affect humans and animals. These diseases are intimately associated with conformational conversion of the cellular prion protein, PrP(C), into an oligomeric beta-sheet-rich form, Pr...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi900108v
更新日期:2009-03-31 00:00:00
abstract::A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982328z
更新日期:1999-03-16 00:00:00
abstract::Limited digestion of polysomal RNA with pancreatic ribonuclease releases a structure consisting of poly(A) associated with other polyribonucleotide sequences. This complex can be purified by oligo(dT)-cellulose chromatography. Heating for formamide treatment causes the dissociation of fragments free of poly(A) from th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00686a024
更新日期:1975-07-29 00:00:00
abstract::Pineapple stem bromelain was photooxidized in the presence of Methylene Blue used as a sensitizer. The essential sulfhydryl group of the enzyme protein rapidly became inaccessible to react with 5,5'-dithiobis(nitrobenzoic acid), but the reactivity was readily regained to the original level upon treatment with dithioth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00673a009
更新日期:1975-01-28 00:00:00
abstract::The product of yjeK in Escherichia coli is a homologue of lysine 2,3-aminomutase (LAM) from Clostridium subterminale SB4, and both enzymes catalyze the isomerization of (S)- but not (R)-alpha-lysine by radical mechanisms. The turnover number for LAM from E. coli is 5.0 min(-1), 0.1% of the value for clostridial LAM. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061328t
更新日期:2006-10-24 00:00:00
abstract::The UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase, encoded by lpxA, catalyzes the first step of lipid A biosynthesis in Gram-negative bacteria, the (R)-3-hydroxyacyl-ACP-dependent acylation of the 3-OH group of UDP-GlcNAc. Recently, we demonstrated that the Arabidopsis thaliana orthologs of six enzymes of the b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3002242
更新日期:2012-05-29 00:00:00
abstract::The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011001m
更新日期:2001-09-25 00:00:00
abstract::Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00655a017
更新日期:1976-05-18 00:00:00
abstract::The egg jelly that encapsulates amphibian eggs is essential for fertilization, but its molecular composition and roles remain largely unknown. We identified a calcium-dependent lectin from the pentraxin superfamily in the egg jelly coat from the South American burrowing frog, Lepidobatrachus laevis. This lectin, jeltr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035314o
更新日期:2003-11-11 00:00:00
abstract::Room temperature phosphorescence techniques were used to study the structural and dynamic features of the tryptophan residues in bovine alpha-crystallin. Upon excitation at 290 nm, the characteristic signature of tryptophan phosphorescence was observed with an emission maximum at 442 +/- 2 nm. The phosphorescence inte...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a027
更新日期:1989-06-27 00:00:00
abstract::Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00174a046
更新日期:1994-03-01 00:00:00
abstract::Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00175a040
更新日期:1994-03-08 00:00:00
abstract::Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00579a015
更新日期:1979-06-12 00:00:00
abstract::The binding of epidermal growth factor (EGF) to epidermal growth factor receptor (EGF receptor) induces dimerization of the receptor and activation of its protein tyrosine kinase. Each of these three steps was followed as a function of the concentrations of EGF and of EGF receptor. Binding of EGF was followed by sedim...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9602268
更新日期:1996-05-07 00:00:00
abstract::Serine hydroxymethyltransferase (SHMT) catalyzes the reversible conversion of l-serine and tetrahydrofolate into glycine and 5,10-methylenetetrahydrofolate. This enzyme, which plays a pivotal role in one-carbon metabolism, is involved in cancer metabolic reprogramming and is a recognized target of chemotherapy interve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01074
更新日期:2018-12-26 00:00:00
abstract::Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00685a027
更新日期:1975-07-15 00:00:00
abstract::The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00043a018
更新日期:1995-10-31 00:00:00
abstract::We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0107187
更新日期:2001-07-31 00:00:00
abstract::Fibronectin is composed of multiple homologous repeats and contains many functional domains. Two major heparin-binding domains have previously been identified: the Hep I site near the amino terminus and the Hep II site near the carboxyl terminus. The Hep II site has been considered the high-affinity heparin-binding si...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991624o
更新日期:2000-03-28 00:00:00
abstract::A dark state tertiary structure in the cytoplasmic domain of rhodopsin is presumed to be the key to the restriction of binding of transducin and rhodopsin kinase to rhodopsin. Upon light-activation, this tertiary structure undergoes a conformational change to form a new structure, which is recognized by the above prot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010746p
更新日期:2001-10-23 00:00:00
abstract::35S-labeled derivatives of the insecticides nodulisporic acid and ivermectin were synthesized and demonstrated to bind with high affinity to a population of receptors in Drosophila head membranes that were previously shown to be associated with a glutamate-gated chloride channel. Nodulisporic acid binding was modeled ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015920o
更新日期:2002-05-21 00:00:00
abstract::A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0001675
更新日期:2000-05-23 00:00:00
abstract::Peptide deformylase (EC 3.5.1.31) catalyzes the removal of a formyl group from the N-termini of nascent ribosome-synthesized polypeptides, an obligatory step during protein maturation in eubacteria. Since its discovery in crude Escherichia coli extracts 3 decades ago, the deformylase has resisted all attempts of purif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971155v
更新日期:1997-11-11 00:00:00
abstract::The thermotropic behavior of aqueous dispersions of palmitoylsphingomyelin-cholesterol and lignoceryl-sphingomyelin-cholesterol mixtures has been examined by high-sensitivity differential scanning calorimetry. When less than 25 mol % cholesterol is mixed with either sphingomyelin, the calorimetric endotherm is compose...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00577a042
更新日期:1979-05-15 00:00:00
abstract::The sucrose permease (CscB) and lactose permease (LacY) of Escherichia coli belong to the oligosaccharide/H(+) symporter subfamily of the major facilitator superfamily, and both catalyze sugar/H(+) symport across the cytoplasmic membrane. Thus far, there is no common substrate for the two permeases; CscB transports su...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201592y
更新日期:2011-12-27 00:00:00
abstract::The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00142a009
更新日期:1992-07-14 00:00:00
abstract::Receptor tyrosine kinase (RTK) activation is associated with modulation of heptahelical receptor-stimulated adenylyl cyclase responses. The mechanisms underlying the RTK-mediated enhancement of adenylyl cyclase function remain unclear. In the present studies, we show that the tyrosine kinase-dependent enhancement of a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0015818
更新日期:2001-02-13 00:00:00
abstract::Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00584a028
更新日期:1979-08-21 00:00:00
abstract::We have measured the hydration of CO2 catalyzed by bovine red cell carbonic anhydrase in mixtures of H2O and D2O at pH(D) which is in the plateau region of the pH--activity profile. The steady-state parameters for hydration Vmax and Km both show an exponential dependence on the atom fraction of deuterium in solvent wa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00563a008
更新日期:1980-10-28 00:00:00
abstract::Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00435a014
更新日期:1989-05-02 00:00:00