Abstract:
:Limited digestion of polysomal RNA with pancreatic ribonuclease releases a structure consisting of poly(A) associated with other polyribonucleotide sequences. This complex can be purified by oligo(dT)-cellulose chromatography. Heating for formamide treatment causes the dissociation of fragments free of poly(A) from the poly(A)-containing components. The two types of fragments tend to reassociate under annealing conditions, and this association is prevented by poly(U). Control experiments indicate that this structure is not an artifact generated during the manipulations. The same structure can be obtained by limited RNase digestion of polyribosomes, followed by deproteinization. The results suggest that the mRNA in polyribosomes may have a defined configuration caused by the interaction of the poly(A) sequence with another segment of the RNA.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Jeffery WR,Brawerman Gdoi
10.1021/bi00686a024subject
Has Abstractpub_date
1975-07-29 00:00:00pages
3445-51issue
15eissn
0006-2960issn
1520-4995journal_volume
14pub_type
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