Abstract:
:The repeating units of mouse, rat, and alpha-monkey satellites have been cloned. All three show properties that are characteristic of curved DNA: (i) their migration in polyacrylamide gels is slower than predicted from their sequences, and (ii) they appear as curved molecules when visualized by electron microscopy. All three satellite repeats contain runs of d(A.T)n greater than or equal to 3 residues that are likely to be responsible for their curvature. From analysis of 20 different satellite DNA sequences, we conclude that, in satellite DNA, adenine residues show a high tendency to cluster in groups of three or more.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Martínez-Balbás A,Rodríguez-Campos A,García-Ramírez M,Sainz J,Carrera P,Aymamí J,Azorín Fdoi
10.1021/bi00461a019subject
Has Abstractpub_date
1990-03-06 00:00:00pages
2342-8issue
9eissn
0006-2960issn
1520-4995journal_volume
29pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The coat protein of bacteriophage Pf3 forms discrete and stable ion channels of uniform size in planar bilayers of asolectin. Its primary sequence suggests a channel formed by a bundle of transmembrane helices. Since the apparent transmembrane region only consists of strongly hydrophobic residues, it represents a new ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00167a037
更新日期:1994-01-11 00:00:00
abstract::The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00120a013
更新日期:1992-02-11 00:00:00
abstract::Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00844
更新日期:2019-11-19 00:00:00
abstract::Database searches indicated that the genome of Bacillus subtilis contains three different genes encoding RNase H homologues. The ypdQ gene encodes an RNase HI homologue with 132 amino acid residues, whereas the rnh and ysgB genes encode RNase HII homologues with 255 and 313 amino acid residues, respectively. RNases HI...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982207z
更新日期:1999-01-12 00:00:00
abstract::A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0102252
更新日期:2001-06-05 00:00:00
abstract::The ion binding parameters of the EF-hand Ca2+ binding motif are carefully tuned for different biological applications. The present study examines the contribution of the ninth position of the Ca2+-coordinating EF-loop to the tuning of Ca2+ affinity and selectivity, using the model EF-loop of the Escherichia coli gala...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952430l
更新日期:1996-05-28 00:00:00
abstract::The membrane-active, cationic, β-hairpin peptide, arenicin, isolated from marine polychaeta Arenicola marina exhibits a broad spectrum of antimicrobial activity. The peptide in aqueous solution adopts the significantly twisted β-hairpin conformation without pronounced amphipathicity. To assess the mechanism of arenici...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200746t
更新日期:2011-07-19 00:00:00
abstract::The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00657a032
更新日期:1976-06-15 00:00:00
abstract::Human telomeric DNA consists of tandem repeats of the DNA sequence d(GGGTTA). Oligodeoxynucleotide telomere models such as d[A(GGGTTA)(3)GGG] (Tel22) fold in a cation-dependent manner into quadruplex structures consisting of stacked G-quartets linked by d(TTA) loops. NMR has shown that in Na(+) solutions Tel22 forms a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901357r
更新日期:2010-01-12 00:00:00
abstract::Ubiquitin was modified with the anionic, arginine-specific reagent 4-(oxoacetyl)phenoxyacetic acid in order to study the relationship between structure and function of the molecule. Four different derivatives (A, B, C, and D) were purified from the reaction mixture by anion-exchange high-performance liquid chromatogra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00396a019
更新日期:1987-11-03 00:00:00
abstract::The influenza virus RNA-dependent RNA polymerase protein complex contains an associated RNA endonuclease activity, which cleaves host mRNA precursors in the cell nucleus at defined positions 9-15 nucleotides downstream of the cap structure. This reaction provides capped oligoribonucleotides, which function as primers ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9828932
更新日期:1999-04-27 00:00:00
abstract::Displacement of the fluorescent substrate analogue methylanthraniloyl ADP (mant-ADP) from kinesin by excess ATP results in a biphasic fluorescent transient. The pH and microtubule dependence of the rates and amplitudes indicates that the two phases are produced by release of bound mant-ADP, with an excess of the 3'-is...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972742j
更新日期:1998-04-14 00:00:00
abstract::Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035424v
更新日期:2004-01-20 00:00:00
abstract::Four-coordinate metalloporphyrins activate soluble guanylyl cyclase. Ni(II)PPIX and Cu(II)PPIX are high affinity activators, with activation constants of 24 and 17 nM, respectively. Both metalloporphyrins remain stably bound to the enzyme, enabling spectroscopic characterization of the Ni(II)- and Cu(II)-reconstituted...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010777k
更新日期:2002-08-06 00:00:00
abstract::The kinetics of tert-butyl isocyanide binding to the heme protein horseradish peroxidase (HRP) at 22 degrees C was examined on all time scales, from minutes to picoseconds, in aqueous borate buffer at pH 9.08. Unlike myoglobin (Mb) or hemoglobin, HRP shows two bimolecular ligand binding processes. For comparison, bind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9518149
更新日期:1996-02-06 00:00:00
abstract::Azurin from Pseudomonas aeruginosa and two mutants where the methionine ligand has been mutated have been studied in order to directly investigate the functional and structural significance of this ligand in the blue copper proteins. Reduction potentials, X-ray absorption fine structure (XAFS), electron paramagnetic r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a013
更新日期:1993-03-02 00:00:00
abstract::With excitation at 406.7 nm, we have observed the resonance Raman enhancement of the bound v(N-O) stretch at approximately 1623 cm-1 in nitrosylhemoglobin A and nitrosylmyoglobin, indicating the existence of a charge-transfer transition underlying the strong Soret band. The v(Fe-NO) stretch at 551 cm-1 has also been d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00535a005
更新日期:1982-03-16 00:00:00
abstract::The purL gene of Escherichia coli encoding the enzyme formylglycinamidine ribonucleotide (FGAM) synthetase which catalyzes the conversion of formylglycinamide ribonucleotide (FGAR), glutamine, and MgATP to FGAM, glutamate, ADP, and Pi has been cloned and sequenced. The mature protein, as deduced by the structural gene...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00432a017
更新日期:1989-03-21 00:00:00
abstract::An investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus (TtPFK) reveals an enzyme that is inhibited by PEP and activated by ADP by modifying the affinity exhibited for the substrate fructose 6-phosphate (Fru-6-P) in a manner...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401402j
更新日期:2014-01-14 00:00:00
abstract::To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0014094
更新日期:2000-12-19 00:00:00
abstract::The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidity of the active site zinc ion. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00358a028
更新日期:1986-05-20 00:00:00
abstract::Transmembrane chemoreceptors, also known as methyl-accepting chemotaxis proteins (MCPs), translate extracellular signals into intracellular responses in the bacterial chemotaxis system. MCP ligand binding domains control the activity of the CheA kinase, situated approximately 200 A away, across the cytoplasmic membran...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801727m
更新日期:2009-03-10 00:00:00
abstract::The newly described adapter molecule p130 Crk-associated substrate (Cas) has been reported to contribute to cytoskeletal organization through assembly of actin filaments and to be pivotal in embryonic development and in oncogene-mediated transformation. We characterized the regulation of Cas tyrosine phosphorylation i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991849z
更新日期:2000-05-16 00:00:00
abstract::The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900958x
更新日期:2009-09-01 00:00:00
abstract::During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A reso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981152y
更新日期:1998-09-01 00:00:00
abstract::The annexin fold consists of four 70-residue domains with markedly homologous sequences and nearly identical structures. Each domain contains five helices designated A to E. Domain 2 of annexin I was obtained by chemical synthesis including ten specifically labeled residues and studied by 1H-15N NMR and circular dichr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960747v
更新日期:1996-08-13 00:00:00
abstract::Photochemical and subsequent thermal reactions of rhodopsin containing 9-cis-retinal [Rh(9)] or one of four analogues with 9-cis geometries formed from ring-modified retinals, alpha-retinal [alpha Rh(9)], acyclic retinal [AcRh(9)], acyclic alpha-retinal [Ac alpha Rh(9)], and 5-isopropyl-alpha-retinal [P alpha Rh(9)] w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00233a022
更新日期:1991-05-14 00:00:00
abstract::4-Azido-2-nitrophenyl pyrophosphate (azido-PPi) labeled with 32P in the alpha position was prepared and used to photolabel beef heart mitochondrial F1. Azido-PPi was hydrolyzed by yeast inorganic pyrophosphatase, but not by mitochondrial F1-ATPase. Incubation of F1 with [alpha-32P]azido-PPi in the dark under condition...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00452a021
更新日期:1989-12-26 00:00:00
abstract::We have studied the role of amphipathic alpha-helices in the ability of apoA-I to promote cholesterol efflux from human skin fibroblasts and activate lecithin:cholesterol acyltransferase (LCAT). Three apoA-I mutants were designed, each by deletion of a pair of predicted adjacent central alpha-helices [Delta(100-143), ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981205b
更新日期:1998-09-29 00:00:00
abstract::Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, pr...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.7b00529
更新日期:2017-10-03 00:00:00