Abstract：
:The coat protein of bacteriophage Pf3 forms discrete and stable ion channels of uniform size in planar bilayers of asolectin. Its primary sequence suggests a channel formed by a bundle of transmembrane helices. Since the apparent transmembrane region only consists of strongly hydrophobic residues, it represents a new class of channel-forming proteins. The channel activity is strongly voltage-dependent. The single-channel conductance of 60 pS (at 100 mV) in 0.2 M NaCl is slightly voltage-dependent, indicating conformational changes of the pore upon variation of the transmembrane electric field. The channel is unselective which suggests that the pore is of aqueous character. For the observed conductance, a channel diameter of 3.6 A is consistent with a tetrameric alpha-helix bundle, as calculated from a barrel-stave model. A pronounced dependence of the gating kinetics with increasing voltage arises from two opposing effects: an increase in the number of open channel structures, and a simultaneous, more than 3-fold decrease in the channel lifetime. Thus, a maximum activity is reached around 100 mV, a range which corresponds well with physiological membrane potentials. The channels activate only upon application of a positive voltage on the side of the membrane to which the protein had been added. The slow relaxation of the mean current upon application of sudden voltage jumps indicates a strong activation barrier in the channel gating process, which may result from the membrane translocation of the charged residues of the peptide ends. A channel-mediated import mechanism is suggested for the bacterial infection by phage DNA.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pawlak M,Kuhn A,Vogel Hdoi
10.1021/bi00167a037subject
Has Abstractpub_date
1994-01-11 00:00:00pages
283-90issue
1eissn
0006-2960issn
1520-4995journal_volume
33pub_type
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