Role of two acetylcholine receptor subunit domains in homomer formation and intersubunit recognition, as revealed by alpha 3 and alpha 7 subunit chimeras.


:Differential expression of subunit genes from the nicotinic acetylcholine receptor (AChR) superfamily yields distinct receptor subtypes. As each AChR subtype has a specific subunit composition and many subunit combinations appear not to be expressed, each subunit must contain some information leading to proper assembly. The neuronal AChR subunits alpha 3 and alpha 7 are expressed in bovine chromaffin cells, probably as constituents of two different AChR subtypes. These subunits have different assembly behavior when expressed in heterologous expression systems: alpha 7 subunits are able to produce homomeric AChRs, whereas alpha 3 subunits require other "structural" subunits for functional expression of AChRs. This feature allows the dissection of the requirements for subunit interactions during AChR formation. Analysis of alpha 7/alpha 3 chimeric constructs identified two regions essential to homomeric assembly and intersubunit recognition: an N-terminal extracellular region, controlling the initial association between subunits, and a second domain within a region comprising the first putative transmembrane segment, M1, and the cytoplasmic loop coupling it to the pore-forming segment, M2, involved in the subsequent interaction and stabilization of the oligomeric complex.






García-Guzmán M,Sala F,Sala S,Campos-Caro A,Criado M




Has Abstract


1994-12-20 00:00:00












  • Role of the K-channel in the pH-dependence of the reaction of cytochrome c oxidase with hydrogen peroxide.

    abstract::The reaction of cytochrome c oxidase (COX) from Rhodobacter sphaeroides with hydrogen peroxide has been studied at alkaline (pH 8.5) and acidic (pH 6.5) conditions with the aid of a stopped-flow apparatus. Absorption changes in the entire 350-800 nm spectral range were monitored and analyzed by a global fitting proced...


    pub_type: 杂志文章


    authors: Pecoraro C,Gennis RB,Vygodina TV,Konstantinov AA

    更新日期:2001-08-14 00:00:00

  • Hepatitis C virus NS3 and simian virus 40 T antigen helicases displace streptavidin from 5'-biotinylated oligonucleotides but not from 3'-biotinylated oligonucleotides: evidence for directional bias in translocation on single-stranded DNA.

    abstract::Helicases are enzymes that use energy from nucleoside triphosphate hydrolysis to unwind double-stranded (ds) DNA, a process vital to virtually every phase of DNA metabolism. Helicases have been classified as either 5'-to-3' or 3'-to-5' on the basis of their ability to unwind duplex DNA adjacent to either a 5' or 3' si...


    pub_type: 杂志文章


    authors: Morris PD,Byrd AK,Tackett AJ,Cameron CE,Tanega P,Ott R,Fanning E,Raney KD

    更新日期:2002-02-19 00:00:00

  • Relationship between the native-state hydrogen exchange and folding pathways of a four-helix bundle protein.

    abstract::The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding ...


    pub_type: 杂志文章


    authors: Chu R,Pei W,Takei J,Bai Y

    更新日期:2002-06-25 00:00:00

  • Interaction of cytochrome c with flavocytochrome b2.

    abstract::Flavocytochrome b2 from Saccharomyces cerevisiae couples L-lactate dehydrogenation to cytochrome c reduction. At 25 degrees C, 0.10 M ionic strength, and saturating L-lactate concentration, the turnover rate is 207 s-1 [per cytochrome c reduced; Miles, C. S., Rouviere, N., Lederer, F., Mathews, F. S., Reid, G. A., Bla...


    pub_type: 杂志文章


    authors: Daff S,Sharp RE,Short DM,Bell C,White P,Manson FD,Reid GA,Chapman SK

    更新日期:1996-05-21 00:00:00

  • Heme-CO religation in photolyzed hemoglobin: a time-resolved Raman study of the Fe-CO stretching mode.

    abstract::Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...


    pub_type: 杂志文章


    authors: Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

    更新日期:1993-02-09 00:00:00

  • Spectroscopic, calorimetric, and kinetic demonstration of conformational adaptation in peptide-antibody recognition.

    abstract::Little is known about the extent to which protein flexibility contributes to antigen-antibody recognition and cross-reactivity. Using short coil peptides (leucine zippers) as model antigens, we demonstrate that a monoclonal antibody can force a noncognate peptide into a conformation that is similar to the conformation...


    pub_type: 杂志文章


    authors: Leder L,Berger C,Bornhauser S,Wendt H,Ackermann F,Jelesarov I,Bosshard HR

    更新日期:1995-12-19 00:00:00

  • Covalent binding of thrombin to specific sites on corneal endothelial cells.

    abstract::Binding of 125I-labeled human alpha-thrombin to endothelial cells derived from bovine corneas was studied in tissue culture. Specific and saturable binding to the cell surface occurred at 37 degrees C but to a much smaller extent at 4 degrees C. Binding of [125I]thrombin to a specific site on these cells with formatio...


    pub_type: 杂志文章


    authors: Isaacs JD,Savion N,Gospodarowicz D,Fenton JW 2nd,Shuman MA

    更新日期:1981-01-20 00:00:00

  • Functional tyrosyl residues of carboxypeptidase A. The effect of protein structure on the reactivity of tyrosine-198.

    abstract::Coupling of bovine carboxypeptidase A with diazotized 5-amino-1H-tetrazole increases esterase activity, decreases peptidase activity slightly, and modifies one tyrosyl residue. Subsequent nitration of the azoenzyme has no further effect on esterase activity, decreases peptidase activity markedly, and modifies a second...


    pub_type: 杂志文章


    authors: Cueni L,Riordan JF

    更新日期:1978-05-16 00:00:00

  • Product dependence of deuterium isotope effects in enzyme-catalyzed reactions.

    abstract::Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...


    pub_type: 杂志文章


    authors: Cook PF,Yoon MY,Hara S,McClure GD Jr

    更新日期:1993-02-23 00:00:00

  • Conformational changes in the cytoplasmic domain of the Escherichia coli aspartate receptor upon adaptive methylation.

    abstract::By using targeted disulfide cross-linking, we have characterized structural changes that the Escherichia coli aspartate receptor undergoes upon modification of the four specific residues that are reversibly methylated during sensory adaptation. Cysteine residues were introduced at specific positions either in the cyto...


    pub_type: 杂志文章


    authors: Le Moual H,Quang T,Koshland DE Jr

    更新日期:1998-10-20 00:00:00

  • Differential release of cellular and scrapie prion proteins from cellular membranes by phosphatidylinositol-specific phospholipase C.

    abstract::The abnormal isoform of the scrapie prion protein PrPSc is both a host-derived protein and a component of the infectious agent causing scrapie. PrPSc and the normal cellular isoform PrPC have different physical properties that apparently arise from a posttranslational event. Both PrP isoforms are covalently modified a...


    pub_type: 杂志文章


    authors: Stahl N,Borchelt DR,Prusiner SB

    更新日期:1990-06-05 00:00:00

  • NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins.

    abstract::Our earlier NMR study showed that a two-disulfide variant of hen lysozyme containing intra-alpha-domain disulfide bridges, C6-C127 and C30-C115, is partially folded, with the alpha domain tightly folded to the nativelike conformation and the beta domain flexible or unfolded. With a view that the formation of a third d...


    pub_type: 杂志文章


    authors: Yokota A,Hirai K,Miyauchi H,Iimura S,Noda Y,Inoue K,Akasaka K,Tachibana H,Segawa S

    更新日期:2004-06-01 00:00:00

  • Glutathione Directly Intercepts DNA Radicals To Inhibit Oxidative DNA-Protein Cross-Linking Induced by the One-Electron Oxidation of Guanine.

    abstract::Oxidative DNA damage can lead to cancer, and as enzymatic DNA repair systems become compromised during the aging process, the role of exogenous antioxidants becomes more critical. Here, we examined whether such non-enzymatic DNA repair can be effected by the common cellular antioxidant glutathione, investigating both ...


    pub_type: 杂志文章


    authors: Safaeipour M,Jauregui J,Castillo S,Bekarian M,Esparza D,Sanchez M,Stemp EDA

    更新日期:2019-11-19 00:00:00

  • Crystal and molecular structure of a new Z-DNA crystal form: d[CGT(2-NH2-A)CG] and its platinated derivative.

    abstract::The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essenti...


    pub_type: 杂志文章


    authors: Parkinson GN,Arvanitis GM,Lessinger L,Ginell SL,Jones R,Gaffney B,Berman HM

    更新日期:1995-11-28 00:00:00

  • Exchange of histidine spacing between Sp1 and GLI zinc fingers: distinct effect of histidine spacing-linker region on DNA binding.

    abstract::In the DNA recognition mode of C(2)H(2)-type zinc fingers, the finger-finger connection region, consisting of the histidine spacing (HX(3-5)H) and linker, would be important for determining the orientation of the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNA binding,...


    pub_type: 杂志文章


    authors: Shiraishi Y,Imanishi M,Sugiura Y

    更新日期:2004-05-25 00:00:00

  • Stereochemical Consequences of Vinylpyruvate Hydratase-Catalyzed Reactions.

    abstract::A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...


    pub_type: 杂志文章


    authors: Johnson WH Jr,Stack TM,Taylor SM,Burks EA,Whitman CP

    更新日期:2016-07-26 00:00:00

  • Cholesterol modulates interaction between an amphipathic class A peptide, Ac-18A-NH2, and phosphatidylcholine bilayers.

    abstract::Cholesterol (Chol) in phosphatidylcholine large unilamellar vesicles (PC LUV) modulated interaction of the bilayers with a class A amphipathic peptide, Ac-18A-NH2: Chol increased the peptide binding capacity and reduced the affinity together with the peptide-induced leakage of calcein from LUV. Similar effects of Chol...


    pub_type: 杂志文章


    authors: Egashira M,Gorbenko G,Tanaka M,Saito H,Molotkovsky J,Nakano M,Handa T

    更新日期:2002-03-26 00:00:00

  • Interaction of alamethicin with lecithin bilayers: a 31P and 2H NMR study.

    abstract::The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...


    pub_type: 杂志文章


    authors: Banerjee U,Zidovetzki R,Birge RR,Chan SI

    更新日期:1985-12-17 00:00:00

  • Rate and extent of poly(ethylene glycol)-induced large vesicle fusion monitored by bilayer and internal contents mixing.

    abstract::Poly(ethylene glycol) (PEG) of average molecular weight 8000 was used to mediate the fusion of large unilamellar vesicles composed of dipalmitoylphosphatidylcholine. Fusion was monitored by fluorescence assays of lipid mixing and aqueous contents mixing. The extent of lipid mixing, as monitored by DPHpPC fluorescence ...


    pub_type: 杂志文章


    authors: Parente RA,Lentz BR

    更新日期:1986-10-21 00:00:00

  • Rudimentary phosvitin domain in a minor chicken vitellogenin gene.

    abstract::We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...


    pub_type: 杂志文章


    authors: Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

    更新日期:1989-03-21 00:00:00

  • A Fold-Independent Interface Residue Is Crucial for Complex Formation and Allosteric Signaling in Class I Glutamine Amidotransferases.

    abstract::The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...


    pub_type: 杂志文章


    authors: Semmelmann F,Hupfeld E,Heizinger L,Merkl R,Sterner R

    更新日期:2019-06-04 00:00:00

  • A common catalytic mechanism for proteins of the HutI family.

    abstract::Imidazolonepropionase (HutI) (imidazolone-5-propanote hydrolase, EC is a member of the amidohydrolase superfamily and catalyzes the conversion of imidazolone-5-propanoate to N-formimino-L-glutamate in the histidine degradation pathway. We have determined the three-dimensional crystal structures of HutI from A...


    pub_type: 杂志文章


    authors: Tyagi R,Eswaramoorthy S,Burley SK,Raushel FM,Swaminathan S

    更新日期:2008-05-20 00:00:00

  • Elimination of the hydrolytic water molecule in a class A beta-lactamase mutant: crystal structure and kinetics.

    abstract::Two site-directed mutant enzymes of the class A beta-lactamase from Staphylococcus aureus PC1 were produced with the goal of blocking the site that in the native enzyme is occupied by the proposed hydrolytic water molecule. The crystal structures of these two mutant enzymes, N170Q and N170M, have been determined and r...


    pub_type: 杂志文章


    authors: Zawadzke LE,Chen CC,Banerjee S,Li Z,Wäsch S,Kapadia G,Moult J,Herzberg O

    更新日期:1996-12-24 00:00:00

  • Isothermal titration calorimetric studies on the binding of deoxytrimannoside derivatives with artocarpin: implications for a deep-seated combining site in lectins.

    abstract::The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...


    pub_type: 杂志文章


    authors: Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

    更新日期:2000-09-05 00:00:00

  • Characterization of the 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase from Xanthobacter strain Py2: product inhibition, pH dependence of kinetic parameters, site-directed mutagenesis, rapid equilibrium inhibition, and chemical modification.

    abstract::Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...


    pub_type: 杂志文章


    authors: Clark DD,Ensign SA

    更新日期:2002-02-26 00:00:00

  • Structural and functional analysis of NADPH-cytochrome P-450 reductase from human liver: complete sequence of human enzyme and NADPH-binding sites.

    abstract::The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...


    pub_type: 杂志文章


    authors: Haniu M,McManus ME,Birkett DJ,Lee TD,Shively JE

    更新日期:1989-10-17 00:00:00

  • Probing the communication between the regulatory and catalytic domains of a protein tyrosine kinase, Csk.

    abstract::Protein tyrosine kinases (PTKs) are important regulators of mammalian cell function and their own activities are tightly regulated. Underlying their tight regulation, all PTKs contain multiple regulatory domains in addition to a catalytic domain. C-terminal Src kinase (Csk) contains a catalytic domain and a regulatory...


    pub_type: 杂志文章


    authors: Lin X,Ayrapetov MK,Lee S,Parang K,Sun G

    更新日期:2005-02-08 00:00:00

  • Thermodynamic characterization of deoxyribooligonucleotide duplexes containing bulges.

    abstract::Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...


    pub_type: 杂志文章


    authors: LeBlanc DA,Morden KM

    更新日期:1991-04-23 00:00:00

  • Characterization of prostate-specific antigen proteolytic activity on its major physiological substrate, the sperm motility inhibitor precursor/semenogelin I.

    abstract::The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...


    pub_type: 杂志文章


    authors: Robert M,Gibbs BF,Jacobson E,Gagnon C

    更新日期:1997-04-01 00:00:00

  • Conformational properties of B-Z junctions in DNA.

    abstract::The structural consequences of specific base sequences in DNA can exert a strong influence on the function of DNA. It has previously been reported that the presence of multiple B-Z conformational junctions in constructed DNA oligomers results in unusually enhanced electrophoretic gel mobilities of these oligomers [Win...


    pub_type: 杂志文章


    authors: Lu M,Guo Q,Kallenbach NR,Sheardy RD

    更新日期:1992-05-19 00:00:00