Polypeptide clearing in model membranes: an analysis of the partition of gramicidin a' between cadmium ion induced gel and liquid-crystalline phases in vesicles of phosphatidic acid and phosphatidylcholine.

abstract：:Bacterial resistance to the aminoglycoside antibiotics is manifested primarily through the production of enzymes which covalently modify these drugs. The Enterococci and Staphylococci produce an ATP-dependent kinase, APH(3')-IIIa, which phosphorylates such antibiotics as kanamycin, amikacin, and neomycin, and this enz...

journal_title：Biochemistry

authors： McKay GA,Wright GD

更新日期：1996-07-02 00:00:00

abstract：:Human insulin-like growth factors I and II (hIGF-I, hIGF-II) are potent stimulators of cell and growth processes. They display high sequence similarity to both the A and B chains of insulin but contain an additional connecting C-domain, which reflects their secretion without specific packaging or precursor conversion....

journal_title：Biochemistry

authors： Brzozowski AM,Dodson EJ,Dodson GG,Murshudov GN,Verma C,Turkenburg JP,de Bree FM,Dauter Z

更新日期：2002-07-30 00:00:00

abstract：:Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...

journal_title：Biochemistry

authors： Xu Y,Lu Z

更新日期：2004-12-14 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:In the ATP-dependent carboxylation of biotin that is catalyzed by most biotin-dependent carboxylases, a fundamental mechanistic question is whether the ATP activates bicarbonate (via the formation of carboxyphosphate as an intermediate) or whether the ATP activates biotin (via the formation of O-phosphobiotin). We hav...

journal_title：Biochemistry

authors： Ogita T,Knowles JR

更新日期：1988-10-18 00:00:00

abstract：:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...

journal_title：Biochemistry

authors： Brenner DG,Knowles JR

更新日期：1984-11-20 00:00:00

abstract：:We have developed a homogeneous time-resolved fluorescence resonance energy transfer (FRET)-based assay that detects the formation of HIV-1 integrase (IN) dimers. The assay utilizes IN monomers that express two different epitope tags that are recognized by their respective antibodies, coupled to distinct fluorophores....

journal_title：Biochemistry

authors： Tsiang M,Jones GS,Hung M,Samuel D,Novikov N,Mukund S,Brendza KM,Niedziela-Majka A,Jin D,Liu X,Mitchell M,Sakowicz R,Geleziunas R

更新日期：2011-03-15 00:00:00

abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

journal_title：Biochemistry

authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

更新日期：2004-12-21 00:00:00

abstract：:The soluble domains of the b and b' subunits of the ATP synthase of the cyanobacterium Synechocystis PCC 6803 were expressed with His tags attached to their N-termini. Following purification, the polypeptides were characterized by chemical cross-linking, analytical ultracentrifugation, and circular dichroism spectrosc...

journal_title：Biochemistry

authors： Dunn SD,Kellner E,Lill H

更新日期：2001-01-09 00:00:00

abstract：:Structural and biochemical characterization of aspartate 152 at the distal heme side of catalase-peroxidase (KatG) from Synechocystis PCC 6803 reveals an important functional role for this residue. In the wild-type protein, the side chain carboxyl group of Asp152 is 7.8 A apart from the heme iron and is hydrogen-bonde...

journal_title：Biochemistry

authors： Jakopitsch C,Auer M,Regelsberger G,Jantschko W,Furtmüller PG,Rüker F,Obinger C

更新日期：2003-05-13 00:00:00

abstract：:Campylobacter jejuni contains a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier and subsequently transferred to the asparagine side chain of an acceptor protein. The enzymes in the pathway function at a membrane interface and have in ...

journal_title：Biochemistry

authors： Chen MM,Weerapana E,Ciepichal E,Stupak J,Reid CW,Swiezewska E,Imperiali B

更新日期：2007-12-18 00:00:00

abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...

journal_title：Biochemistry

authors： Techtmann SM,Ghirlando R,Kao S,Strebel K,Maynard EL

更新日期：2012-03-13 00:00:00

abstract：:The expression of rusticyanin in Escherichia coli and a number of mutants for Ser86 is reported. Mutations of Ser86 to Asn, Asp, Gln, and Leu were undertaken as this is an Asn residue in other structurally characterized cupredoxins, and it has been suggested that this may be partly responsible for the high redox poten...

journal_title：Biochemistry

authors： Hall JF,Kanbi LD,Harvey I,Murphy LM,Hasnain SS

更新日期：1998-08-18 00:00:00

abstract：:The role of the "C-terminal tail" segment of long neurotoxins has been investigated. The C-terminal four to five residues of alpha-bungarotoxin and Laticauda colubrina b have been cleaved off by carboxypeptidase P. The effect of such deletion on the toxin conformation has been monitored in proton nuclear magnetic reso...

journal_title：Biochemistry

authors： Endo T,Oya M,Tamiya N,Hayashi K

更新日期：1987-07-14 00:00:00

abstract：:Overall activation volumes for multistep reactions are not usually pressure independent. The present investigation gives a quantitative description of this effect under Theory. Simple relations are obtained which can easily be applied to experimental data and which allow more insight into the dynamics of enzyme reacti...

journal_title：Biochemistry

authors： Butz P,Greulich KO,Ludwig H

更新日期：1988-03-08 00:00:00

abstract：:The active site of water oxidation in Photosystem II (PSII) is a Mn4CaO5 cluster that is located in a cavity between the D1 and CP43 protein subunits by which it is coordinated. The remainder of this cavity is filled with water molecules, which serve as a source of substrate and participate in poorly understood hydrog...

journal_title：Biochemistry

authors： Dilbeck PL,Bao H,Neveu CL,Burnap RL

更新日期：2013-10-01 00:00:00

abstract：:Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...

journal_title：Biochemistry

authors： Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

更新日期：1995-09-12 00:00:00

abstract：:A type II topoisomerase is essential for decatenating DNA replication products, and it accomplishes this task by passing one DNA duplex through a transient break in a second duplex. The B' domain of topoisomerase II contains three highly conserved motifs, EGDSA, PL(R/K)GK(I/L/M)LNVR, and IMTD(Q/A)DXD. We have investig...

journal_title：Biochemistry

authors： West KL,Meczes EL,Thorn R,Turnbull RM,Marshall R,Austin CA

更新日期：2000-02-15 00:00:00

abstract：:Several peptides contained within the C-terminal sequence of the B2 subunit of Escherichia coli ribonucleotide reductase (RNR) were investigated for their ability to inhibit the enzyme, presumably by interfering with association of the B1 and B2 subunits. AcYLVGQIDSE, corresponding by sequence homology to a nonapeptid...

journal_title：Biochemistry

authors： Bushweller JH,Bartlett PA

更新日期：1991-08-20 00:00:00

abstract：:The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...

journal_title：Biochemistry

authors： Perona JJ,Tsu CA,Craik CS,Fletterick RJ

更新日期：1997-05-06 00:00:00

abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...

journal_title：Biochemistry

authors： Smith PA,Jackson V,Chalkley R

更新日期：1984-03-27 00:00:00

abstract：:A new actinoleukin-like antitumor antibiotic, BBM-928A, has been shown to interact with isolated DNA molecules. BBM-928A contains two substituted quinolines linked by a cyclic decapeptide. Quenching effects of the covalently closed superhelical PM2 DNA on the BBM-928A fluorescence revealed a strong interaction with an...

journal_title：Biochemistry

authors： Huang CH,Mong S,Crooke ST

更新日期：1980-11-25 00:00:00

abstract：:Molecules that bind DNA via threading intercalation show high binding affinity as well as slow dissociation kinetics, properties ideal for the development of anticancer drugs. To this end, it is critical to identify the specific molecular characteristics of threading intercalators that result in optimal DNA interactio...

journal_title：Biochemistry

authors： Clark AG,Naufer MN,Westerlund F,Lincoln P,Rouzina I,Paramanathan T,Williams MC

更新日期：2018-02-06 00:00:00

abstract：:The effect of protonation events on the charge equilibrium between tyrosine-D and the water-oxidizing complex in photosystem II has been studied by time-resolved measurements of the EPR signal IIslow at room temperature. The flash-induced oxidation of YD by the water-oxidizing complex in the S2 state is a monophasic p...

journal_title：Biochemistry

authors： Vass I,Styring S

更新日期：1991-01-22 00:00:00

abstract：:The stereoselectivity of the adenosine cyclic 3',5'-phosphate (cAMP) binding sites on the regulatory subunit of the type II bovine cardiac muscle cAMP-dependent protein kinase was investigated by examining the interactions of (Rp)- and (Sp)-adenosine cyclic 3',5'-phosphorothioates (cAMPS) with these sites. While activ...

journal_title：Biochemistry

authors： O'Brian CA,Roczniak SO,Bramson HN,Baraniak J,Stec WJ,Kaiser ET

更新日期：1982-08-31 00:00:00

abstract：:To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142),...

journal_title：Biochemistry

authors： Gorshkova IN,Liu T,Kan HY,Chroni A,Zannis VI,Atkinson D

更新日期：2006-01-31 00:00:00

abstract：:Human placental lipocortin is a high-affinity substrate for rat brain protein kinase C in vitro with phosphorylation occurring on serine and threonine residues in a ratio of approximately 2 to 1. Comparison of the ability of various N-terminal-truncated derivatives of lipocortin to serve as phosphorylation substrates,...

journal_title：Biochemistry

authors： Schlaepfer DD,Haigler HT

更新日期：1988-06-14 00:00:00

abstract：:Phosphorylation by ATP of E.*Ca2 (sarcoplasmic reticulum vesicles (SRV) with bound 45Ca2+) during 5-10 ms leads to the occlusion of 2 *Ca2+/EPtot [quench by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) alone] in both "empty" (10 microM free Ca2+in) or "loaded" SRV (20-40 mM free Ca2+in)...

journal_title：Biochemistry

authors： Khananshvili D,Jencks WP

更新日期：1988-04-19 00:00:00

abstract：:The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...

journal_title：Biochemistry

authors： Kayden HJ,Hatam L,Beratis NG

更新日期：1976-02-10 00:00:00

abstract：:The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...

journal_title：Biochemistry

authors： Huang HW

更新日期：2000-07-25 00:00:00