Catalytic mechanism of enterococcal kanamycin kinase (APH(3')-IIIa): viscosity, thio, and solvent isotope effects support a Theorell-Chance mechanism.

abstract：:We have carried out a comparative study of the effect of cholesterol on the thermotropic phase behavior of the distearoyl and dielaidoyl molecular species of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine using high-sensitivity differential scanning calorimetry. For both molecular species of pho...

journal_title：Biochemistry

authors： McMullen TP,McElhaney RN

更新日期：1997-04-22 00:00:00

abstract：:Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...

journal_title：Biochemistry

authors： Glaser PE,Gross RW

更新日期：1995-09-26 00:00:00

abstract：:Isoform 1 of the mammalian Na(+)/H(+) exchanger (NHE1) is a ubiquitously expressed plasma membrane pH regulatory protein. It removes one intracellular H(+) in exchange for one extracellular Na(+). The 500 N-terminal amino acids comprise the catalytic membrane domain and fold into 12 transmembrane (TM) segments. To gai...

journal_title：Biochemistry

authors： Alves C,Lee BL,Sykes BD,Fliegel L

更新日期：2014-06-10 00:00:00

abstract：:We find that specific oxidation for the Met-192 residue in delta-chymotrypsin to methionine sulfoxide results in a twofold increase in Km(app) and unchanged kcat in the hydrolysis of N-acetyl mono(amino acid) amide substrates. However, the catalyzed hydrolyses of N-acetyl dipeptide amide substrates by (methionine sulf...

journal_title：Biochemistry

authors： Treadway WJ Jr,Schultz RM

更新日期：1976-09-21 00:00:00

abstract：:Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...

journal_title：Biochemistry

authors： Geng CD,Vedeckis WV

更新日期：2010-10-19 00:00:00

abstract：:Haemadin is a 57-amino acid thrombin inhibitor from the land-living leech Haemadipsa sylvestris, whose structure has recently been determined in complex with human alpha-thrombin. Here we communicate the effect of ionic strength on the kinetics of the inhibition of human alpha-thrombin by haemadin, by using thrombin m...

journal_title：Biochemistry

authors： Richardson JL,Fuentes-Prior P,Sadler JE,Huber R,Bode W

更新日期：2002-02-26 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:The class I human liver alcohol dehydrogenases (ADHs) catalyze the interconversion of the intermediary alcohols and aldehydes of dopamine metabolism in vitro, whereas those of the class II and class III do not. The individual, homogeneous class I isozymes oxidize (3,4-dihydroxyphenyl)ethanol and (4-hydroxy-3-methoxyph...

journal_title：Biochemistry

authors： Mårdh G,Vallee BL

更新日期：1986-11-18 00:00:00

abstract：:The transferrins (TFs) are a family of proteins that are widely distributed in vertebrates, where they serve a major role in iron binding and transport. Most TFs are composed of two homologous lobes, the N- and C-lobes, each able to bind a single iron atom. Human serum transferrin (hTF) binds iron in the blood and del...

journal_title：Biochemistry

authors： Halbrooks PJ,He QY,Briggs SK,Everse SJ,Smith VC,MacGillivray RT,Mason AB

更新日期：2003-04-08 00:00:00

abstract：:The orphan receptor short heterodimer partner (SHP) is a common partner for a great number of nuclear receptors, and it plays an important role in many diverse physiological events. In a previous study, we described SHP as a strong repressor of the androgen receptor (AR). Herein, we addressed the mechanism of action o...

journal_title：Biochemistry

authors： Gobinet J,Carascossa S,Cavaillès V,Vignon F,Nicolas JC,Jalaguier S

更新日期：2005-04-26 00:00:00

abstract：:Currently, it is thought that inhalational anesthetics cause anesthesia by binding to ligand-gated ion channels. This is being investigated using four-alpha-helix bundles, small water-soluble analogues of the transmembrane domains of the "natural" receptor proteins. The study presented here specifically investigates h...

journal_title：Biochemistry

authors： Manderson GA,Michalsky SJ,Johansson JS

更新日期：2003-09-30 00:00:00

abstract：:We have previously designed and synthesized small-molecule inhibitors that reduce Vibrio cholerae virulence in vitro by targeting the transcription factor ToxT. Here we report the synthesis and biological activity of derivatives of our previous bicyclic, fatty acid-like inhibitors. All of the synthesized derivatives s...

journal_title：Biochemistry

authors： Woodbrey AK,Onyango EO,Kovacikova G,Kull FJ,Gribble GW

更新日期：2018-09-25 00:00:00

abstract：:During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast AD...

journal_title：Biochemistry

authors： Plapp BV,Savarimuthu BR,Ferraro DJ,Rubach JK,Brown EN,Ramaswamy S

更新日期：2017-07-18 00:00:00

abstract：:Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...

journal_title：Biochemistry

authors： Booth V,Waring AJ,Walther FJ,Keough KM

更新日期：2004-12-07 00:00:00

abstract：:Five previously unknown hemorrhagic proteins, designated hemorrhagic toxins a,b,c,d, and e, were isolated from the venom of the western diamondback rattlesnake (Crotalus atrox). Molecular weights of hemorrhagic toxins a-e were determined to be 68 000, 24 000, 24 000, 24 000, and 25 700, respectively, by sodium dodecyl...

journal_title：Biochemistry

authors： Bjarnason JB,Tu AT

更新日期：1978-08-08 00:00:00

abstract：:Studies of ligand binding to acetylcholinesterase (AChE) have demonstrated two sites of interaction. An acyl-enzyme intermediate is formed at the acylation site, and catalytic activity can be inhibited by ligand binding to a peripheral site. The three-dimensional structures of AChE-ligand complexes reveal a narrow and...

journal_title：Biochemistry

authors： Mallender WD,Szegletes T,Rosenberry TL

更新日期：2000-07-04 00:00:00

abstract：:Pyruvate decarboxylase (PDC) uses thiamine diphosphate as an essential cofactor to catalyze the formation of acetaldehyde on the pathway of ethanol synthesis. Here we report the crystallographic image of a prereaction intermediate of a bacterial pyruvate decarboxylase prepared by cocrystallizing the enzyme with pyruva...

journal_title：Biochemistry

authors： Pei XY,Erixon KM,Luisi BF,Leeper FJ

更新日期：2010-03-02 00:00:00

abstract：:The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...

journal_title：Biochemistry

authors： Haddad JG,Hu YZ,Kowalski MA,Laramore C,Ray K,Robzyk P,Cooke NE

更新日期：1992-08-11 00:00:00

abstract：:We have used DNase I footprinting to examine the formation of antiparallel DNA triple helices on DNA fragments containing the homopurine target sites (GGA)2GGX(GGA)2GG.(CCT)2CCZ(CCT)2CC (where X.Z is each base pair in turn), with the GA- and GT-rich oligonucleotides, (GGA)2GGN(GGA)2GG and (GGT)2GGN(GGT)2GG (N = each b...

journal_title：Biochemistry

authors： Chandler SP,Fox KR

更新日期：1996-11-26 00:00:00

abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...

journal_title：Biochemistry

authors： Chan WW,Mosbach K

更新日期：1976-09-21 00:00:00

abstract：:The expression of the transforming gene product of Rous sarcoma virus (pp60v-src) in Saccharomyces cerevisiae has recently been reported (Kornbluth et al., 1987; Brugge et al., 1987). To carry out biochemical and structural studies of this enzyme, a facile purification was developed. The purification was accomplished ...

journal_title：Biochemistry

authors： Radziejewski C,Miller WT,Mobashery S,Goldberg AR,Kaiser ET

更新日期：1989-11-14 00:00:00

abstract：:Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) ...

journal_title：Biochemistry

authors： Payne G,Sancar A

更新日期：1990-08-21 00:00:00

abstract：:The solution structure of the complementary d(C1-A2-T3-G4-X5-G6-T7-A8-C9).d(G10-T11-A12-C13-A14-C15-A16-T17-G18) DNA duplex (designated X.A 9-mer), which contains a 1,N2-propanodeoxyguanosine exocyclic adduct X5 opposite deoxyadenosine A14 at the center, is pH dependent [Kouchakdjian, M., Eisenberg, M., Live, D., Mari...

journal_title：Biochemistry

authors： Huang P,Patel DJ,Eisenberg M

更新日期：1993-04-20 00:00:00

abstract：:Lon is a homo-oligomeric ATP-dependent serine protease that functions in the degradation of damaged and certain regulatory proteins. This enzyme has emerged as a novel target in the development of antibiotics because of its importance in conferring bacterial virulence. In this study, we explored the mechanism by which...

journal_title：Biochemistry

authors： Frase H,Lee I

更新日期：2007-06-05 00:00:00

abstract：:The dynamics of individual DNA molecules undergoing orthogonal field alternating gel electrophoresis (OFAGE) have been studied by use of T2 DNA molecules labeled with a dye and visualized with a fluorescence microscope. The mechanism of reorientation used by a molecule to align itself in the direction of the new ortho...

journal_title：Biochemistry

authors： Gurrieri S,Rizzarelli E,Beach D,Bustamante C

更新日期：1990-04-03 00:00:00

abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...

journal_title：Biochemistry

authors： Shaw GS,Sykes BD

更新日期：1996-06-11 00:00:00

abstract：:Adriamycin plays a prominent role in the treatment of leukemia and solid tumors in man. The mode of interaction of adriamycin with its nuclear target, responsible for its therapeutic effect, is known [Berman, H. M., & Young, P.R. (1981) Annu. Rev. Biophys. Bioeng. 10, 87-114]. The planar anthracycline moiety of adriam...

journal_title：Biochemistry

authors： Goormaghtigh E,Brasseur R,Huart P,Ruysschaert JM

更新日期：1987-03-24 00:00:00

abstract：:Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(...

journal_title：Biochemistry

authors： Larsson AM,Bergfors T,Dultz E,Irwin DC,Roos A,Driguez H,Wilson DB,Jones TA

更新日期：2005-10-04 00:00:00

abstract：:We report two small molecule azobenzenesulfonamide probes, CAP1 and CAP2, capable of photomodulating the activity of carbonic anhydrase (CA) on demand. In the trans form, CAP azobenzene probes adopt a linear shape, making them suitable for occupying the CA active site and interacting with Zn2+, thereby inhibiting enzy...

journal_title：Biochemistry

authors： Aggarwal K,Banik M,Medellin B,Que EL

更新日期：2019-01-08 00:00:00

abstract：:Fluorescence resonance energy transfer (FRET) has been used to investigate the conformation of the single stranded region for a series of fluorescent DNA template-primers bound to the Klenow fragment (KF) of Escherichia coli DNA polymerase I. Fluorescent derivatives of template-primer DNA, modified with tetramethylrho...

journal_title：Biochemistry

authors： Furey WS,Joyce CM,Osborne MA,Klenerman D,Peliska JA,Balasubramanian S

更新日期：1998-03-03 00:00:00