Abstract：
:Several peptides contained within the C-terminal sequence of the B2 subunit of Escherichia coli ribonucleotide reductase (RNR) were investigated for their ability to inhibit the enzyme, presumably by interfering with association of the B1 and B2 subunits. AcYLVGQIDSE, corresponding by sequence homology to a nonapeptide that inhibits herpes simplex RNR [Gaudreau et al. (1987) J. Biol. Chem. 262, 12413] shows no inhibition of the E. coli enzyme (IC50 greater than 3 mM), whereas AcDDLSNFQL, the C-terminal octapeptide of the E. coli B2 subunit, is a noncompetitive inhibitor (Ki = 160 microM). Neither bradykinin (RPPGFSPFR) nor the pentapeptide AcSNFQL inhibits the E. coli enzyme. Transferred nuclear Overhauser enhancement spectroscopy was used to probe the conformation of AcDDLSNFQL when it is bound to the B1 subunit. These experiments suggest that the peptide adopts a turn in the region of Asn5 and Phe6 and that a hydrophobic cluster of the phenylalanine and leucine side chains is involved in the interaction surface.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bushweller JH,Bartlett PAdoi
10.1021/bi00247a008subject
Has Abstractpub_date
1991-08-20 00:00:00pages
8144-51issue
33eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00652a031
更新日期：1976-04-06 00:00:00
abstract：:Geobacillus stearothermophilus T-6 encodes for a beta-xylosidase (XynB2) from family 52 of glycoside hydrolases that was previously shown to hydrolyze its substrate with net retention of the anomeric configuration. XynB2 significantly prefers substrates with xylose as the glycone moiety and exhibits a typical bell-sha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034505o
更新日期：2003-09-09 00:00:00
abstract：:Mouse photoreceptor function and survival critically depend on Ca(2+)-regulated retinal membrane guanylyl cyclase (RetGC), comprised of two isozymes, RetGC1 and RetGC2. We characterized the content, catalytic constants, and regulation of native RetGC1 and RetGC2 isozymes using mice lacking guanylyl cyclase activating ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200491b
更新日期：2011-06-28 00:00:00
abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9520141
更新日期：1996-01-30 00:00:00
abstract：:1-Deoxy-D-fructose was synthesized in 27% yield from D-glucosamine in a three-step procedure involving Raney nickel desulfurization and oxidative deamination with 3,5-di-tert-butyl- 1,2-benzoquinone applied to appropriate intermediates. 1-Deoxyfructose and its reduction products, 1-deoxyglucitol and 1-deoxymannitol, w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00665a027
更新日期：1976-10-05 00:00:00
abstract：:Hydroxyproline-rich glycoproteins (HRGPs) are the major proteinaceous components of higher plant walls and the predominant components of the cell wall of the green alga Chlamydomonas reinhardtii. The GP1 protein, an HRGP of the C. reinhardtii wall, is shown to adopt a polyproline II helical configuration and to carry ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0023605
更新日期：2001-03-06 00:00:00
abstract：:The reversible inhibition of beta-lactamase I from Bacillus cereus by cloxacillin, methicillin, and nafcillin has been systematically investigated. For these substrates the enzymatic reaction involves partitioning of the substrate between turnover and inhibition. Typically, concentrations of several hundred millimolar...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00388a011
更新日期：1987-07-14 00:00:00
abstract：:Cyanide inhibits the CO oxidation activity of carbon monoxide dehydrogenase from Clostridium thermoaceticum by binding tightly to the form of the C-cluster yielding the gav = 1.82 signal (the C1.82 form). CN- dissociates and the enzyme reactivates upon addition of CO, CO2 plus dithionite, or CS2 plus dithionite. Dithi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00195a011
更新日期：1994-07-26 00:00:00
abstract：:To generate an effective immune response, class II major histocompatibility complex molecules (MHCII) must present a diverse array of peptide ligands for recognition by T lymphocytes. Peptide/MHCII complexes are stabilized by hydrophobic anchoring of peptide side chains to pockets in the MHCII protein and the formatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048675s
更新日期：2005-04-19 00:00:00
abstract：:The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase. In this work, we employed stopped-flow optical absorption spectroscopy to study the kinetic behavior of the Michaelis complex of C...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00179
更新日期：2020-08-04 00:00:00
abstract：:We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00225a026
更新日期：1991-03-19 00:00:00
abstract：:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00555a043
更新日期：1980-07-08 00:00:00
abstract：:Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00551a005
更新日期：1980-05-13 00:00:00
abstract：:We characterized endocytosis of iron-saturated (holo) and iron-depleted (apo) 125I-labeled bovine lactoferrin (Lf) by isolated rat hepatocytes. Hepatocytes ingested both Lf forms--determined by EGTA/dextran sulfate removal of surface-bound Lf--at maximal endocytic rates of 1.85 and 1.52 fmol cell-1 min-1 for 125I-apo-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00212a046
更新日期：1993-12-14 00:00:00
abstract：:We have developed a new genomic sequencing method for detecting, with resolution at the nucleotide level, the interstrand DNA cross-links induced by 4,5',8-trimethylpsoralen along single-copy genes in mammalian cells. The cross-links (diadducts) initially formed are converted into monoadducts by alkali reversal prior ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002539f
更新日期：2001-04-03 00:00:00
abstract：:Mycobacterium tuberculosis and many other members of the Actinomycetes family produce mycothiol, i.e., 1-d-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-d-glucopyranoside (MSH or AcCys-GlcN-Ins), to act against oxidative and antibiotic stress. The biosynthesis of MSH is essential for cell growth and has bee...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7011492
更新日期：2007-10-09 00:00:00
abstract：:Human lysine demethylase KDM5A is a chromatin-modifying enzyme associated with transcriptional regulation, because of its ability to catalyze removal of methyl groups from methylated lysine 4 of histone H3 (H3K4me3). Amplification of KDM5A is observed in many cancers, including breast cancer, prostate cancer, hepatoce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b01036
更新日期：2020-02-11 00:00:00
abstract：:Alpha-synuclein plays a key role in the pathogenesis of many neurodegenerative diseases. To date, its cellular role has yet to be determined, although it has been proposed to be connected to calcium and G protein-mediated dopamine signaling. Alpha-synuclein is known to bind strongly to model membrane surfaces where it...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0487140
更新日期：2005-01-18 00:00:00
abstract：:The iminodihydroquinoline WIN 17317-3 was previously shown to inhibit selectively the voltage-gated potassium channels, K(v)1.3 and K(v)1.4 [Hill, R. J., et al. (1995) Mol. Pharmacol. 48, 98-104; Nguyen, A., et al. (1996) Mol. Pharmacol. 50, 1672-1679]. Since these channels are found in brain, radiolabeled WIN 17317-3...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990336p
更新日期：1999-08-24 00:00:00
abstract：:Terpene synthases are potentially useful biocatalysts for the synthesis of valuable compounds, such as anticancer drugs and antibiotics. The design of altered activities requires better knowledge of their mechanisms, for example, an understanding of the complex conformational changes that are part of their catalytic c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400898k
更新日期：2013-11-12 00:00:00
abstract：:Calpain-catalyzed proteolysis of II-spectrin is a regulated event associated with neuronal long-term potentiation, platelet and leukocyte activation, and other processes. Calpain proteolysis is also linked to apoptotic and nonapoptotic cell death following excessive glutamate exposure, hypoxia, HIV-gp120/160 exposure,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061504y
更新日期：2007-01-16 00:00:00
abstract：:Beta-1,4-galactosyltransferase (beta4Gal-T1) in the presence of manganese ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either free or linked to an oligosaccharide. Crystallographic studies on bovine beta4Gal-T1 have shown that the primary metal binding site is located in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049007+
更新日期：2004-10-05 00:00:00
abstract：:During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A reso...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981152y
更新日期：1998-09-01 00:00:00
abstract：:Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00133a009
更新日期：1992-05-12 00:00:00
abstract：:When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00268a025
更新日期：1982-12-07 00:00:00
abstract：:We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration from 100 to 500 mM, via replacement of potassium...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8022653
更新日期：2009-07-21 00:00:00
abstract：:Using our hemoglobin expression system in Escherichia coli, we have constructed three recombinant hemoglobins (rHbs) with amino acid substitutions located in the alpha(1)beta(1) and alpha(1)beta(2) subunit interfaces and in the distal heme pocket of the alpha-chain: rHb(alphaV96W, betaN108K), rHb(alphaL29F, alphaV96W,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991271t
更新日期：1999-10-05 00:00:00
abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0114372
更新日期：2001-11-27 00:00:00
abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a002
更新日期：1976-10-19 00:00:00
abstract：:The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00026a022
更新日期：1995-07-04 00:00:00
