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Glycosylated polyproline II rods with kinks as a structural motif in plant hydroxyproline-rich glycoproteins.

Abstract：

:Hydroxyproline-rich glycoproteins (HRGPs) are the major proteinaceous components of higher plant walls and the predominant components of the cell wall of the green alga Chlamydomonas reinhardtii. The GP1 protein, an HRGP of the C. reinhardtii wall, is shown to adopt a polyproline II helical configuration and to carry a complex array of arabinogalactoside residues, many branched, which are necessary to stabilize the helical conformation. The deduced GP1 amino acid sequence displays two Ser-Pro-rich domains, one with a repeating (SP)(x)() motif and the other with a repeating (PPSPX)(x)() motif. A second cloned gene a2 also carries the PPSPX repeat, defining a novel gene family in this lineage. The SP-repeat domains of GP1 form a 100-nm shaft with a flexible kink 28 nm from the head. The gp1 gene encodes a PPPPPRPPFPANTPM sequence at the calculated kink position, generating the proposal that this insert interrupts the PPII helix, with the resultant kink exposing amino acids necessary for GP1 to bind to partner molecules. It is proposed that similar kinks in the higher plant HRGPs called extensins may play a comparable role in wall assembly.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ferris PJ,Woessner JP,Waffenschmidt S,Kilz S,Drees J,Goodenough UW

doi

10.1021/bi0023605

subject

Has Abstract

pub_date

2001-03-06 00:00:00

pages

2978-87

issue

9

eissn

0006-2960

issn

1520-4995

pii

bi0023605

journal_volume

40

pub_type

杂志文章

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