Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication.

abstract：:An investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus (TtPFK) reveals an enzyme that is inhibited by PEP and activated by ADP by modifying the affinity exhibited for the substrate fructose 6-phosphate (Fru-6-P) in a manner...

journal_title：Biochemistry

authors： McGresham MS,Lovingshimer M,Reinhart GD

更新日期：2014-01-14 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:Guided by the X-ray structure analysis of a crystalline EcoRV-d(GGGATATCCC) complex (Winkler, in preparation), we have begun to identify functionally important amino acid residues of EcoRV. We show here that Asn70, Asp74, Ser183, Asn185, Thr186, and Asn188 are most likely involved in the binding and/or cleavage of the...

journal_title：Biochemistry

authors： Thielking V,Selent U,Köhler E,Wolfes H,Pieper U,Geiger R,Urbanke C,Winkler FK,Pingoud A

更新日期：1991-07-02 00:00:00

abstract：:Apolipophorin III (apoLp-III) from the greater wax moth Galleria mellonella is an exchangeable insect apolipoprotein that consists of five amphipathic alpha-helices, sharing high sequence identity with apoLp-III from the sphinx moth Manduca sexta whose structure is available. To define the minimal requirement for apoL...

journal_title：Biochemistry

authors： Dettloff M,Weers PM,Niere M,Kay CM,Ryan RO,Wiesner A

更新日期：2001-03-13 00:00:00

abstract：:A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized QCM in buffer so...

journal_title：Biochemistry

authors： Nihira T,Mizuno M,Tonozuka T,Sakano Y,Mori T,Okahata Y

更新日期：2005-07-12 00:00:00

abstract：:Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...

journal_title：Biochemistry

authors： Giangiacomo KM,Sugg EE,Garcia-Calvo M,Leonard RJ,McManus OB,Kaczorowski GJ,Garcia ML

更新日期：1993-03-09 00:00:00

abstract：:Shaker potassium (K+) channels normally lack intrasubunit and intersubunit disulfide bonds. However, disulfide bonds are formed between Shaker subunits in intact cells exposed to oxidizing conditions. Upon electrophoresis under nonreducing conditions, intersubunit disulfide bond formation was detected by the presence ...

journal_title：Biochemistry

authors： Schulteis CT,Nagaya N,Papazian DM

更新日期：1996-09-17 00:00:00

abstract：:Escherichia coli mannitol specific EII in membrane vesicles can be inhibited by the action of the oxidizable substrate-reduced phenazine methosulfate (PMS) in a manner similar to E. coli enzyme IIGlc [Robillard, G. T., & Konings, W. (1981) Biochemistry 20, 5025-5032]. The fact that reduced PMS and various oxidizing ag...

journal_title：Biochemistry

authors： Roossien FF,Robillard GT

更新日期：1984-01-17 00:00:00

abstract：:Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...

journal_title：Biochemistry

authors： Taylor EL,O'Brien PJ

更新日期：2015-01-27 00:00:00

abstract：:The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...

journal_title：Biochemistry

authors： Bogachev AV,Bertsova YV,Barquera B,Verkhovsky MI

更新日期：2001-06-19 00:00:00

abstract：:Ricin A-chain (RTA) catalyzes the hydrolytic depurination of a specific adenosine at position 4324 of 28S rRNA. Kinetic isotope effects on the hydrolysis of a small 10mer stem-tetraloop oligonucleotide substrate established the mechanism of the reaction as D(N)*A(N), involving an oxacarbenium ion intermediate in a hig...

journal_title：Biochemistry

authors： Roday S,Amukele T,Evans GB,Tyler PC,Furneaux RH,Schramm VL

更新日期：2004-05-04 00:00:00

abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

journal_title：Biochemistry

authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

更新日期：1995-12-05 00:00:00

abstract：:The structure of the subgel phase of dipalmitoylphosphatidylglycerol (DPPG) has been analyzed by X-ray diffraction techniques. Diffraction recorded from highly oriented DPPG specimens in the subgel phase extends to 2-A resolution. There are sharp lamellar reflections on the meridian, and other reflections lie on a ser...

journal_title：Biochemistry

authors： Blaurock AE,McIntosh TJ

更新日期：1986-01-28 00:00:00

abstract：:Recent studies on cytochrome oxidase have indicated that the putative "peroxy" intermediate in the catalytic cycle (P(R)) is a mixture of intermediates, including P and F [Sucheta, A., et al. (1998) Biochemistry 37, 17905-17914], and the bench-made P and F forms appear to have the same redox state (Fe(a3)(4+)=O(2-)), ...

journal_title：Biochemistry

authors： Van Eps N,Szundi I,Einarsdóttir O

更新日期：2003-05-06 00:00:00

abstract：:Blood coagulation is initiated by Ca(2+)-dependent binding of coagulation factor VIIa (FVIIa) to its cofactor, tissue factor (TF). The TF:FVIIa complex activates factors IX and X, ultimately leading to the formation of thrombin and the coagulation of blood. FVII consists of an N-terminal gamma-carboxyglutamic-acid-con...

journal_title：Biochemistry

authors： Muranyi A,Finn BE,Gippert GP,Forsén S,Stenflo J,Drakenberg T

更新日期：1998-07-28 00:00:00

abstract：:Previous experiments have shown that a Synechocystis sp. PCC 6803 mutant (delta psbO) lacking the extrinsic manganese-stabilizing protein (MSP) exhibits impaired, but significant levels of H2O-splitting activity [Burnap, R., & Sherman, L.A. (1991) Biochemistry 30, 440-446]. [14C]DCMU-binding experiments now show that ...

journal_title：Biochemistry

authors： Burnap RL,Shen JR,Jursinic PA,Inoue Y,Sherman LA

更新日期：1992-08-18 00:00:00

abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

journal_title：Biochemistry

authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

更新日期：2002-06-04 00:00:00

abstract：:DNA-binding geometry and dynamics of a number of anthracyclines, including adriamycin and 4-demethoxydaunorubicin, interacting with DNA have been studied by means of linear dichroism and fluorescence techniques. The anthracycline chromophore is found to be approximately parallel to the plane of the DNA bases and to ha...

journal_title：Biochemistry

authors： Eriksson M,Nordén B,Eriksson S

更新日期：1988-10-18 00:00:00

abstract：:The role of histidine in catalysis by prostaglandin H synthase has been investigated using chemical modification with diethyl pyrocarbonate (DEPC), an agent that has been found to rather selectively derivatize histidine residues in proteins under mild conditions. Incubation of the synthase apoprotein with DEPC at pH 7...

journal_title：Biochemistry

authors： Zhang X,Tsai AL,Kulmacz RJ

更新日期：1992-03-10 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

journal_title：Biochemistry

authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

更新日期：2010-01-12 00:00:00

abstract：:Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...

journal_title：Biochemistry

authors： Dwyer JD,Bloomfield VA

更新日期：1982-06-22 00:00:00

abstract：:The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...

journal_title：Biochemistry

authors： Fodor E,Mack JW,Maeng JS,Ju JH,Lee HS,Gruschus JM,Ferretti JA,Ginsburg A

更新日期：2005-09-20 00:00:00

abstract：:The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...

journal_title：Biochemistry

authors： Ashkenazi A,Merklinger E,Shai Y

更新日期：2012-09-04 00:00:00

abstract：:Living organisms have evolved a vast array of catalytic functions that make them ideally suited for the production of medicinally and industrially relevant small-molecule targets. Indeed, native metabolic pathways in microbial hosts have long been exploited and optimized for the scalable production of both fine and co...

journal_title：Biochemistry

authors： Weeks AM,Chang MC

更新日期：2011-06-21 00:00:00

abstract：:The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...

journal_title：Biochemistry

authors： Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

更新日期：1992-12-08 00:00:00

abstract：:NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...

journal_title：Biochemistry

authors： Sari N,Alexander P,Bryan PN,Orban J

更新日期：2000-02-08 00:00:00

abstract：:We have studied the structure of the CuB site in the binuclear heme-copper center of the fully oxidized form of the quinol-oxidizing cytochrome aa3-600 from Bacillus subtilis by EXAFS and ENDOR spectroscopy. This enzyme is member of the large superfamily of heme-copper respiratory oxidases, which catalyze the reductio...

journal_title：Biochemistry

authors： Fann YC,Ahmed I,Blackburn NJ,Boswell JS,Verkhovskaya ML,Hoffman BM,Wikström M

更新日期：1995-08-15 00:00:00

abstract：:Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...

journal_title：Biochemistry

authors： Pan KM,Damodaran S,Greaser ML

更新日期：1994-07-12 00:00:00

abstract：:Structures of several voltage-gated sodium (NaV) channels from bacteria have been determined recently, but the same feat might not be achieved for the mammalian counterparts in the near future. Thus, at present, computational studies of the mammalian NaV channels have to be performed using homology models based on the...

journal_title：Biochemistry

authors： Patel D,Mahdavi S,Kuyucak S

更新日期：2016-03-29 00:00:00

abstract：:A novel selective calcium channel antagonist peptide, SNX-325, has been isolated from the venom of the spider Segestria florentina. The peptide was isolated using as bioassays the displacement of radioiodinated omega-conopeptide SNX-230 (MVIIC) from rat brain synaptosomal membranes, as well as the inhibition of the ba...

journal_title：Biochemistry

authors： Newcomb R,Palma A,Fox J,Gaur S,Lau K,Chung D,Cong R,Bell JR,Horne B,Nadasdi L

更新日期：1995-07-04 00:00:00