An N-terminal three-helix fragment of the exchangeable insect apolipoprotein apolipophorin III conserves the lipid binding properties of wild-type protein.

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:The present paper reports a study on the equilibria and kinetics of the acid-alkaline transition and the azide binding reaction by ferric Aplysia myoglobin. A single completely reversible spectrophotometric titration curve is found over the pH range from similar to 5 to similar to 9, with an apparent pK equals to 7.5 ...

journal_title：Biochemistry

authors： Giacometti GM,Da Ros A,Antonini E,Brunori M

更新日期：1975-04-22 00:00:00

abstract：:An alamethicin, secreted by the fungus Trichoderma viride and containing a glutamine at position 18 instead of the usual glutamic acid, has been uniformly labeled with 15N and purified by HPLC. The extent of 15N incorporation at individual backbone and side-chain sites was found to vary from 85% to 92%, as measured by...

journal_title：Biochemistry

authors： Yee AA,O'Neil JD

更新日期：1992-03-31 00:00:00

abstract：:The carboxypeptidase inhibitor from Russet Burbank potatoes (C. A. Ryan et al. (1974b), J. Biol. Chem 249, 5495) is a mixture of approximately equal amounts of two polypeptide chains containing 38 and 39 amino acid residues, respectively. The chains differ in their amino terminal sequence only, one beginning with smal...

journal_title：Biochemistry

authors： Hass GM,Nau H,Biemann K,Grahn DT,Ericsson LH,Neurath H

更新日期：1975-03-25 00:00:00

abstract：:The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...

journal_title：Biochemistry

authors： Gibbons WA,Beyer CF,Dadok J,Sprecher RF,Wyssbrod HR

更新日期：1975-01-28 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is a transmembrane protein involved in cell signaling processes, and dysregulation of its activity often drives tumor growth. EGFR is a clinically validated tumor marker and target for antibodies and tyrosine kinase inhibitors. We demonstrate that a fusion protein of the nat...

journal_title：Biochemistry

authors： Feiner RC,Pennè I,Müller B,Müller KM

更新日期：2019-02-26 00:00:00

abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...

journal_title：Biochemistry

authors： Ahmad F,Jacobson B,Chuang M,Brattin W,Wood HG

更新日期：1975-04-22 00:00:00

abstract：:Fluorescein isothiocyanate derivatization of the human lactotransferrin on Lys-264 inhibits the binding of the protein of human PHA-activated lymphocytes [Legrand, D., Mazurier, J., Maes, P., Rochard, E., Montreuil, J., & Spik, G. (1991) Biochem. J. 276, 733-738], indicating that part of the receptor-binding site is l...

journal_title：Biochemistry

authors： Legrand D,Mazurier J,Elass A,Rochard E,Vergoten G,Maes P,Montreuil J,Spik G

更新日期：1992-09-29 00:00:00

abstract：:The influenza M2 proton channel is a major drug target, but unfortunately, the acquisition of resistance mutations greatly reduces the functional life span of a drug in influenza treatment. New M2 inhibitors that inhibit mutant M2 channels otherwise resistant to the early adamantine-based drugs have been reported, but...

journal_title：Biochemistry

authors： Santner P,Martins JMDS,Kampmeyer C,Hartmann-Petersen R,Laursen JS,Stein A,Olsen CA,Arkin IT,Winther JR,Willemoës M,Lindorff-Larsen K

更新日期：2018-10-16 00:00:00

abstract：:A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...

journal_title：Biochemistry

authors： Seward HE,Basran J,Denton R,Pfuhl M,Muskett FW,Bagshaw CR

更新日期：2013-04-09 00:00:00

abstract：:Among the molecular strategies bacteria have set up to quickly match their transcriptional program to new environments, changes in sequence-mediated DNA curvature play a crucial role. Bacterial promoters, especially those of mesophilic bacteria, are in general preceded by a curved region. The marked thermosensitivity ...

journal_title：Biochemistry

authors： Prosseda G,Mazzola A,Di Martino ML,Tielker D,Micheli G,Colonna B

更新日期：2010-04-06 00:00:00

abstract：:Apo horse spleen ferritin (apo HoSF) was reconstituted to various core sizes (100-3500 Fe3+/HoSF) by depositing Fe(OH)3 within the hollow HoSF interior by air oxidation of Fe2+. Fe2+ and phosphate (Pi) were then added anaerobically at a 1:4 ratio, and both Fe2+ and Pi were incorporated into the HoSF cores. The resulti...

journal_title：Biochemistry

authors： Johnson JL,Cannon M,Watt RK,Frankel RB,Watt GD

更新日期：1999-05-18 00:00:00

abstract：:The redox properties of the [2Fe-2S] cluster in the 24 kDa subunit of bovine heart mitochondrial NADH:ubiquinone oxidoreductase (complex I) and three of its homologues have been defined using protein-film voltammetry. The clusters in all four examples display characteristic, pH-dependent redox transitions, which, unus...

journal_title：Biochemistry

authors： Zu Y,Di Bernardo S,Yagi T,Hirst J

更新日期：2002-08-06 00:00:00

abstract：:Carbonic anhydrase (CA) catalyzes the reversible hydration of carbon dioxide to hydrogen carbonate, and its role in maintaining pH balance has made it an attractive drug target. Steroidal sulfamate esters, inhibitors of the cancer drug target steroid sulfatase (STS), are sequestered in vivo by CA II in red blood cells...

journal_title：Biochemistry

authors： Cozier GE,Leese MP,Lloyd MD,Baker MD,Thiyagarajan N,Acharya KR,Potter BV

更新日期：2010-04-27 00:00:00

abstract：:The influenza virus RNA-dependent RNA polymerase protein complex contains an associated RNA endonuclease activity, which cleaves host mRNA precursors in the cell nucleus at defined positions 9-15 nucleotides downstream of the cap structure. This reaction provides capped oligoribonucleotides, which function as primers ...

journal_title：Biochemistry

authors： Doan L,Handa B,Roberts NA,Klumpp K

更新日期：1999-04-27 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Like phytochromes, CBCRs photointerconvert between two photostates that accompany photoisomerization of their bilin chromophores. While phytochromes typically exhibit red/far-red photocycles, CBCR photocycles are much mor...

journal_title：Biochemistry

authors： Gottlieb SM,Kim PW,Corley SC,Madsen D,Hanke SJ,Chang CW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

更新日期：2014-02-18 00:00:00

abstract：:The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reac...

journal_title：Biochemistry

authors： Baas BJ,Poddar H,Geertsema EM,Rozeboom HJ,de Vries MP,Permentier HP,Thunnissen AM,Poelarends GJ

更新日期：2015-02-10 00:00:00

abstract：:The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR st...

journal_title：Biochemistry

authors： London RE,Gabel SA

更新日期：1988-10-04 00:00:00

abstract：:DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...

journal_title：Biochemistry

authors： Jacobs KA,Schlessinger D

更新日期：1977-03-08 00:00:00

abstract：:The endogenous phosphoserine residue in porcine pepsinogen has been titrated with use of phosphorus-31 nuclear magnetic resonance (31P NMR). It has an observed pKa2 of 6.7 and a narrow line width (congruent to 10 Hz). The phosphate can be readily removed by an acid phosphatase from potato; however, it is resistant to ...

journal_title：Biochemistry

authors： Williams SP,Bridger WA,James MN

更新日期：1986-10-21 00:00:00

abstract：:The inhibitory effects of guanosine 5'-(gamma-fluorotriphosphate) [GTP(gamma F)] on both the polymerization and the colchicine-dependent GTPase activity of calf brain tubulin have been studied. The results demonstrate that this analogue of GTP, with a fluorine atom on the gamma-phosphate, is a reversible competitive d...

journal_title：Biochemistry

authors： Monasterio O,Timasheff SN

更新日期：1987-09-22 00:00:00

abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...

journal_title：Biochemistry

authors： Mills M,Völker J,Klump HH

更新日期：1996-10-15 00:00:00

abstract：:Liver alcohol dehydrogenase (LADH) carboxymethylated at Cys-46 (CMLADH) forms two different ternary complexes with 4-trans-(N,N-dimethylamino)cinnamaldehyde (DACA). The complex with reduced nicotinamide adenine dinucleotide (NADH) is characterized by a 38-nm red shift of the long-wavelength pi, pi* transition to 436 n...

journal_title：Biochemistry

authors： Dahl KH,Dunn MF

更新日期：1984-12-18 00:00:00

abstract：:The dissociation of Semliki Forest virus induced by increasing concentrations of the anionic detergent sodium dodecyl sulfate was studied using density gradient centrifugation. Detectable binding to the virus started well below the critical micellar concentration of the detergent and increased thereafter with increase...

journal_title：Biochemistry

authors： Becker R,Helenius A,Simons K

更新日期：1975-05-06 00:00:00

abstract：:During a recent investigation of the persistence of substrate ambiguity in contemporary enzymes, we identified three distinct ambiguous sugar kinases embedded within the modern Escherichia coli genome [Miller, B. G., and Raines, R. T. (2004) Biochemistry 43, 6387-6392]. These catalysts are the YajF, YcfX, and NanK pol...

journal_title：Biochemistry

authors： Miller BG,Raines RT

更新日期：2005-08-16 00:00:00

abstract：:Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...

journal_title：Biochemistry

authors： Spooner PJ,Watts A

更新日期：1991-04-23 00:00:00

abstract：:The peptide hormone bradykinin exerts important biological functions by binding to and activating bradykinin B2 receptors. B2 receptors belong to the seven transmembrane domain (7TM) receptor family. Cloning of the cDNA sequences for the rat, human, and mouse bradykinin B2 receptor revealed several in-frame AUG triple...

journal_title：Biochemistry

authors： AbdAlla S,Godovac-Zimmermann J,Braun A,Roscher AA,Müller-Esterl W,Quitterer U

更新日期：1996-06-11 00:00:00

abstract：:A soluble extract prepared from cells of an Escherichia coli strain carrying the antibiotic resistance plasmid R6K is capable of carrying out the complete process of R6K DNA replication. DNA synthesis in vitro is dependent on the four deoxyribo- and ribonucleotide triphosphates and is sensitive to rifampin and strepto...

journal_title：Biochemistry

authors： Inuzuka M,Helinski DR

更新日期：1978-06-27 00:00:00

abstract：:The mechanisms for substrate recognition by two cytoplasmic protein tyrosine phosphatases, PTP-5 and rrbPTP-1, were investigated. Phosphorylation sites on tyrosine-phosphorylated casein, a model PTP substrate, were characterized. Two peptides based on casein phosphorylation sites and one peptide based on the tyrosine ...

journal_title：Biochemistry

authors： Hippen KL,Jakes S,Richards J,Jena BP,Beck BL,Tabatabai LB,Ingebritsen TS

更新日期：1993-11-23 00:00:00

abstract：:The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...

journal_title：Biochemistry

authors： Doukov T,Li H,Soltis M,Poulos TL

更新日期：2009-11-03 00:00:00