Abstract：
:We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration from 100 to 500 mM, via replacement of potassium with rubidium, or with an increase in the magnesium concentration from 5 to 20 mM. Electron microscopy showed that the polymers assembled under the conditions of fastest assembly were predominantly short, one-stranded protofilaments, whereas under conditions of slower dynamics, the protofilaments tended to associate into long, thin bundles. We suggest that exchange of subunits between protofilaments at steady state involves two separate mechanisms: (1) fragmentation or dissociation of subunits from protofilament ends following GTP hydrolysis and (2) reversible association and dissociation of subunits from protofilament ends independent of hydrolysis. Exchange of nucleotides on these recycling subunits could give the appearance of exchange directly into the polymer. Several of our observations suggest that exchange of nucleotide can take place on these recycling subunits, but not directly into the FtsZ polymer. Annealing of protofilaments was demonstrated for the L68W mutant in EDTA buffer but not in Mg buffer, where rapid cycling of subunits may obscure the effect of annealing. We also reinvestigated the nucleotide composition of FtsZ polymers at steady state. We found that the GDP:GTP ratio was 50:50 for concentrations of GTP >100 microM, significantly higher than the 20:80 ratio previously reported at 20 microM GTP.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chen Y,Erickson HPdoi
10.1021/bi8022653subject
Has Abstractpub_date
2009-07-21 00:00:00pages
6664-73issue
28eissn
0006-2960issn
1520-4995journal_volume
48pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The prion protein (PrP) is a cell-surface Cu2+ binding glycoprotein which can bind six copper ions. The role of Cu2+ in PrP function, misfolding, and prion disease has generated much interest; however, the field has been hampered by a lack of consensus with regard to the affinity of Cu2+ for PrPC. Here we build on our...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9011397
更新日期：2009-09-29 00:00:00
abstract：:The neutral glycosphingolipids of hairy cells from a patient with hairy cell leukemia were chemically analyzed by thin-layer and gas-liquid chromatography, mass spectrometry, combined gas chromatography-mass spectrometry, and glycosidase treatment. These cells were found to have compounds containing one to four sugars...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00525a032
更新日期：1981-10-27 00:00:00
abstract：:The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00341a013
更新日期：1985-09-24 00:00:00
abstract：:The location of the sites of methylation of the methyl-accepting chemotaxis proteins (MCP) of Escherichia coli has been investigated by trypsin digestion and mapping of the peptides. The two principal MCPs, MCP I and MCP II, were found to have very similar methyl-labeled peptide patterns. Each produced three major spe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00507a051
更新日期：1981-02-17 00:00:00
abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0477034
更新日期：2005-04-26 00:00:00
abstract：:The photochemical and subsequent thermal reactions of phoborhodopsin (pR490), which mediates the negative phototaxis (phobic reaction) of Halobacterium halobium, were investigated by low-temperature spectrophotometry. At room temperature, the absorption spectrum of pR490 displayed vibrational structure with a maximum ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00244a008
更新日期：1991-07-30 00:00:00
abstract：:There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951247p
更新日期：1996-03-26 00:00:00
abstract：:The carbon monoxide binding curve to human hemoglobin A0 has been measured to high precision in experimental conditions of 600 microM heme, 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.1 M NaCl, 10 mM inositol hexaphosphate, 1 mM disodium ethylenediaminetetraacetic acid, pH 6.94, and 25 degrees C. Co...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00394a031
更新日期：1987-10-06 00:00:00
abstract：:TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a037
更新日期：1993-04-06 00:00:00
abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7000246
更新日期：2007-06-19 00:00:00
abstract：:Shaker potassium (K+) channels normally lack intrasubunit and intersubunit disulfide bonds. However, disulfide bonds are formed between Shaker subunits in intact cells exposed to oxidizing conditions. Upon electrophoresis under nonreducing conditions, intersubunit disulfide bond formation was detected by the presence ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961083s
更新日期：1996-09-17 00:00:00
abstract：:Adenylosuccinate lyase, an enzyme catalyzing two reactions in purine biosynthesis (the cleavage of either adenylosuccinate or succinylaminoimidazole carboxamide ribotide), has been implicated in a human disease arising from point mutations in the gene encoding the enzyme. Asn(276) of Bacillus subtilis adenylosuccinate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020640+
更新日期：2003-02-25 00:00:00
abstract：:In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7000714
更新日期：2007-08-14 00:00:00
abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01154
更新日期：2019-02-12 00:00:00
abstract：:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900064t
更新日期：2009-06-02 00:00:00
abstract：:Site-directed mutagenesis studies have confirmed that the distal histidine in myoglobin stabilizes bound O2 by hydrogen bonding and have suggested that it is the polar character of the imidazole side chain rather than its size that limits the rate of ligand entry into the protein. We constructed an isosteric Val68 to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00239a025
更新日期：1991-06-25 00:00:00
abstract：:Designing subtype-selective agonists for neuronal nicotinic acetylcholine receptors is a challenging and significant goal aided by intricate knowledge of each subtype's binding patterns. We previously reported that in α6β2 receptors, acetylcholine makes a functional cation-π interaction with Trp149, but nicotine and T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00213
更新日期：2017-04-04 00:00:00
abstract：:Phosphorylation of phosphatidylinositol (PtdIns) by PtdIns 4-kinases is the first step in the synthesis of polyphosphoinositides, the lipid precursors of intracellular signaling molecules. We have recently identified a cytosolic PtdIns 4-kinase (cPI4K) in the bovine adrenal cortex that is distinguished from previously...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9517493
更新日期：1996-03-19 00:00:00
abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2016266
更新日期：2012-01-17 00:00:00
abstract：:The group-specific antigen was isolated from a type Ia group B streptococcal strain and is a complex polysaccharide composed of alpha-L-rhamnopyranosyl, alpha-D-galactopyranosyl, 2-acetamido-2-deoxy-beta-D-glucopyranosyl, D-glucitol, and phosphate residues. The complexity of the group B polysaccharide antigen is evide...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00376a020
更新日期：1987-01-27 00:00:00
abstract：:The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00641a013
更新日期：1977-11-01 00:00:00
abstract：:The structure of a 22 amino acid peptide, TPPI [Nedved, M. L., Gottlieb, P. A., & Moe, G. R. (1994) Nucleic Acids Res. 22, 5024-5030], that is similar to the proline repeat segment of the replication arrest protein, Tus, has been determined by 1H NMR in 50% trifluroethanol. The structure is a novel left-handed helix h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952419l
更新日期：1996-01-23 00:00:00
abstract：:The inflammatory mediator nitric oxide (NO*) promotes apoptotic cell death based on morphological evidence, accumulation of the tumor suppressor p53, caspase-3 activation, and DNA fragmentation in RAW 264.7 macrophages. Since nitrosothiols may actually be the predominant form of biologically active NO* in vivo, we use...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982292a
更新日期：1999-02-23 00:00:00
abstract：:The number of free cysteines in each polypeptide of acetylcholine receptor from the electric organ of Torpedo californica has been assessed by alkylating the native protein with N-ethylmaleimide and iodoacetamide during homogenization of the tissue and alkylating the polypeptides with N-ethylmaleimide as they were unf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00434a048
更新日期：1989-04-18 00:00:00
abstract：:A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00463a025
更新日期：1990-03-20 00:00:00
abstract：:The murine urokinase-type plasminogen activator (uPA) gene has been isolated from a BALB/c liver DNA cosmid library and its nucleotide sequence established. The gene is organized into 11 exons comprising 34.7% of the 6710 base pair (bp) region spanning the interval between the presumed transcription initiation and pol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00399a038
更新日期：1987-12-15 00:00:00
abstract：:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400053s
更新日期：2013-03-12 00:00:00
abstract：:A heparin-induced conformational change is required to convert antithrombin from a slow to a fast inhibitor of factor Xa. It has been proposed [van Boeckel et al. (1994) Nat. Struct. Biol. 1, 423-425] that the reactive center residue P14 is inserted into beta-sheet A in native antithrombin and is displaced from the be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9604643
更新日期：1996-07-02 00:00:00
abstract：:The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00098a023
更新日期：1991-08-27 00:00:00
abstract：:The pathological prion protein PrP(Sc) is the only known component of the infectious prion. In cells infected with prions, PrP(Sc) is formed posttranslationally by the refolding of the benign cell surface glycoprotein PrP(C) into an aberrant conformation. The two PrP isoforms possess very different properties, as PrP(...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025958g
更新日期：2002-10-22 00:00:00
