Abstract：
:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a drug-specific manner. Motions of bases on nanosecond and picosecond time scales are differentially affected by the drug binding. Our results underscore the importance of understanding the detailed dynamic picture of molecular recognition by resolving dynamics in the distinct picosecond time regime and facilitate development of antimicrobial drugs targeting dynamic RNAs.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lu J,Zhao L,Xia A,Xia T,Qi Xdoi
10.1021/bi400053ssubject
Has Abstractpub_date
2013-03-12 00:00:00pages
1651-3issue
10eissn
0006-2960issn
1520-4995journal_volume
52pub_type
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