RuvB protein-mediated ATP hydrolysis: functional asymmetry in the RuvB hexamer.

abstract：:The crystal structure of a nitrogenase Fe protein single site deletion variant reveals a distinctly new conformation of the Fe protein and indicates that, upon binding of MgATP, the Fe protein undergoes a dramatic conformational change that is largely manifested in the rigid-body reorientation of the homodimeric Fe pr...

journal_title：Biochemistry

authors： Sen S,Igarashi R,Smith A,Johnson MK,Seefeldt LC,Peters JW

更新日期：2004-02-24 00:00:00

abstract：:The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...

journal_title：Biochemistry

authors： Pancoska P,Keiderling TA

更新日期：1991-07-16 00:00:00

abstract：:The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase. In this work, we employed stopped-flow optical absorption spectroscopy to study the kinetic behavior of the Michaelis complex of C...

journal_title：Biochemistry

authors： Geng J,Weitz AC,Dornevil K,Hendrich MP,Liu A

更新日期：2020-08-04 00:00:00

abstract：:The dimeric nature of the HIV protease has been exploited to devise a novel mode of inhibiting the enzyme. The use of defective monomers or nonidentical subunits to exchange with wild-type homodimers produces catalytically defective heterodimers. Incubation of the HIV1 or HIV2 protease with a 4-fold molar excess of an...

journal_title：Biochemistry

authors： Babé LM,Pichuantes S,Craik CS

更新日期：1991-01-08 00:00:00

abstract：:Serine hydroxymethyltransferase and C1-tetrahydrofolate synthase catalyze four reactions which convert formate and glycine to serine. The one-carbon carrier in these reactions if tetrahydropteroylglutamate which is regenerated in the coupled reaction and thus can be used in catalytic concentrations with respect to ser...

journal_title：Biochemistry

authors： Strong WB,Schirch V

更新日期：1989-11-28 00:00:00

abstract：:Several 3-halo-3-(1-haloalkyl)-1(3H)-isobenzofuranones, 3-(1-haloalkylidene)-1(3H)-isobenzofuranones, and 3-bromomethyl-1H-2-benzopyran-1-ones containing masked halo ketone functional groups were synthesized and tested as inhibitors of several serine proteases including human leukocyte (HL) elastase and cathepsin G. W...

journal_title：Biochemistry

authors： Hemmi K,Harper JW,Powers JC

更新日期：1985-04-09 00:00:00

abstract：:Lipid species changes for SV40-transformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice were determined for glycerophospholipids, polyphosphatidylinositides (GPInsP n ) and diacylglycerol (DAG) using direct infusion mass spectrome...

journal_title：Biochemistry

authors： Milne SB,Ivanova PT,Armstrong MD,Myers DS,Lubarda J,Shulga YV,Topham MK,Brown HA,Epand RM

更新日期：2008-09-09 00:00:00

abstract：:The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...

journal_title：Biochemistry

authors： Totir MA,Padayatti PS,Helfand MS,Carey MP,Bonomo RA,Carey PR,van den Akker F

更新日期：2006-10-03 00:00:00

abstract：:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. Th...

journal_title：Biochemistry

authors： Szundi I,Funatogawa C,Kliger DS

更新日期：2016-09-13 00:00:00

abstract：:HlyC is an internal protein acyltransferase that activates hemolysin, a toxic protein produced by pathogenic Escherichia coli. Acyl-acyl carrier protein (ACP) is the essential acyl donor. Separately subcloned, expressed, and purified prohemolysin A (proHlyA), HlyC, and [1-14C]myristoyl-ACP have been used to study the ...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-03-16 00:00:00

abstract：:The key polyamine catabolizing enzyme spermidine-spermine N1-acetyltransferase (SSAT) is among the few genes known to be inducible by the natural polyamines. Certain polyamine analogs markedly exaggerate this response and thus provide useful tools for studying the underlying regulatory mechanisms. As shown here, the a...

journal_title：Biochemistry

authors： Fogel-Petrovic M,Vujcic S,Brown PJ,Haddox MK,Porter CW

更新日期：1996-11-12 00:00:00

abstract：:Calf brain tubulin was subjected to isoelectric focusing and tryptic peptide map analysis. Results from isoelectric focusing experiments showed a total number of 17 well-resolved protein peaks. The number of peaks and the mass distribution under each peak remained the same when the concentration of protein or ampholyt...

journal_title：Biochemistry

authors： George HJ,Misra L,Field DJ,Lee JC

更新日期：1981-04-28 00:00:00

abstract：:Ultraviolet irradiation of calmodulin in the presence of calcium results in either the intramolecular cross-linking of Tyr99 and Tyr138 [Malencik, D.A., & Anderson, S.R. (1986) Biochemistry 25, 709] or, when [Tyr8]substance P is bound, the generation of peptide-calmodulin adducts. The latter consist of two chromatogra...

journal_title：Biochemistry

authors： Malencik DA,Anderson SR

更新日期：1988-02-09 00:00:00

abstract：:The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...

journal_title：Biochemistry

authors： Fodor E,Mack JW,Maeng JS,Ju JH,Lee HS,Gruschus JM,Ferretti JA,Ginsburg A

更新日期：2005-09-20 00:00:00

abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

journal_title：Biochemistry

authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

更新日期：2007-03-13 00:00:00

abstract：:Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been previously reported that the soluble extracellular domains of the EPO receptor (sEPOR) are not dimerized in the presence of EPO and are able to form only 1:1 complex...

journal_title：Biochemistry

authors： Philo JS,Aoki KH,Arakawa T,Narhi LO,Wen J

更新日期：1996-02-06 00:00:00

abstract：:A methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) from Clostridium thermoaceticum catalyzes the transfer of the N5 methyl group from (6S)-methyltetrahydrofolate (CH3-H4folate) to the cobalt center of a corrinoid/iron-sulfur protein (C/Fe-SP). The methylcobamide product is the first in a s...

journal_title：Biochemistry

authors： Zhao S,Roberts DL,Ragsdale SW

更新日期：1995-11-21 00:00:00

abstract：:Self-assembly properties of HIV-1 integrase were investigated by time-resolved fluorescence anisotropy using tryptophanyl residues as a probe. From simulation analyses, we show that suitable photon counting leads to an accurate determination of long rotational correlation times in the range of 20-80 ns, permitting the...

journal_title：Biochemistry

authors： Deprez E,Tauc P,Leh H,Mouscadet JF,Auclair C,Brochon JC

更新日期：2000-08-08 00:00:00

abstract：:The effect of Ca2+ on the interaction of bovine cardiac myosin subfragment 1 (S-1) with actin regulated by cardiac troponin-tropomyosin was evaluated. The ratios of actin to troponin and to tropomyosin were adjusted to optimize the Ca2+-dependent regulation of the steady-state actin-activated magnesium adenosinetripho...

journal_title：Biochemistry

authors： Tobacman LS,Adelstein RS

更新日期：1986-02-25 00:00:00

abstract：:The enzyme hydrogenase, from the photosynthetic bacterium Chromatium, was purified to homogeneity after solubilization of the particulate enzyme with deoxycholate. The purification procedure included ammonium sulfate fractionation, treatment with manganous phosphate gel, heating at 63 degrees, DEAE-cellulose chromatog...

journal_title：Biochemistry

authors： Gitlitz PH,Krasna AI

更新日期：1975-06-17 00:00:00

abstract：:Rad51 protein, a eukaryotic homologue of RecA protein, forms a filamentous complex with DNA and catalyzes homologous recombination. We have analyzed the structure of Xenopus Rad51 protein (XRad51.1) in solution by small-angle neutron scattering (SANS). The measurements showed that XRad51.1 forms a helical filament ind...

journal_title：Biochemistry

authors： Ellouze C,Kim HK,Maeshima K,Tuite E,Morimatsu K,Horii T,Mortensen K,Nordén B,Takahashi M

更新日期：1997-11-04 00:00:00

abstract：:Human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2, respectively) exhibit extensive genetic variations. It was postulated that much of this genetic variability stems from the low fidelity of the reverse transcription step. Both HIV reverse transcriptases (RTs) were shown to be particularly error-prone during...

journal_title：Biochemistry

authors： Bakhanashvili M,Hizi A

更新日期：1992-10-06 00:00:00

abstract：:Hepatic lipase (HL) hydrolysis of phosphatidylcholine (PC) was studied in recombinant high-density lipoprotein particles (r-HDL). r-HDL were made from cholate mixed micelles that contained PC, apo AI, and, in some cases, unesterified cholesterol. r-HDL were characterized using chemical composition, nondenaturing gradi...

journal_title：Biochemistry

authors： Tansey JT,Thuren TY,Jerome WG,Hantgan RR,Grant K,Waite M

更新日期：1997-10-07 00:00:00

abstract：:Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoimidazole-4-carboxamide ribonucleotide (FAICAR) to IMP in the final step of de novo purine biosynthesis. Two major types of this enzyme have been discovered to date: PurH in Bacteria and Eukarya and PurO in Archaea. The structure of ...

journal_title：Biochemistry

authors： Kang YN,Tran A,White RH,Ealick SE

更新日期：2007-05-01 00:00:00

abstract：:The cDNA that encodes the proenzyme form of human fibroblast collagenase has been expressed in Escherichia coli. It has been shown by a number of criteria to be functionally identical with the enzyme isolated from human sources. Mutations of each of three cysteine residues found in procollagenase were constructed by s...

journal_title：Biochemistry

authors： Windsor LJ,Birkedal-Hansen H,Birkedal-Hansen B,Engler JA

更新日期：1991-01-22 00:00:00

abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...

journal_title：Biochemistry

authors： Pap EH,Hanicak A,van Hoek A,Wirtz KW,Visser AJ

更新日期：1995-07-18 00:00:00

abstract：:An NMR study has been made of porcine pancreatic phospholipase A2 (PLA) in three environments: free in solution, in a binary complex with dodecylphosphocholine micelles, and in a ternary complex with a micelle and the substrate-like inhibitor (R)-1-octyl-2-(N-dodecanoylamino)-2-deoxyglycero-3-phosph oglycol. 1H and 15...

journal_title：Biochemistry

authors： Peters AR,Dekker N,van den Berg L,Boelens R,Kaptein R,Slotboom AJ,de Haas GH

更新日期：1992-10-20 00:00:00

abstract：:The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...

journal_title：Biochemistry

authors： Yu SS,Ji CZ,Wu YP,Lee TL,Lai CH,Lin SC,Yang ZL,Wang VC,Chen KH,Chan SI

更新日期：2007-12-04 00:00:00

abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...

journal_title：Biochemistry

authors： Craig ML,Suh WC,Record MT Jr

更新日期：1995-12-05 00:00:00

abstract：:The beta 2-adrenergic receptor (beta 2AR) is a member of a large superfamily of seven transmembrane domain, G-protein-coupled receptors. Within the putative seventh transmembrane domain of the beta 2AR is a sequence of amino acids, NPLIY, which is conserved with minor variations in all members of the superfamily. Prev...

journal_title：Biochemistry

authors： Barak LS,Ménard L,Ferguson SS,Colapietro AM,Caron MG

更新日期：1995-11-28 00:00:00