Abstract：
:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. The time-resolved absorption spectra in the 330-650 nm range were analyzed by global exponential and kinetic scheme fitting methods. The results indicate an activation mechanism that is more complex than suggested previously. It involves interconnected branched pathways with two metarhodopsin I480 and two metarhodopsin II intermediates. The intermediates involved in this more complex mechanism need to be considered in spectroscopic studies that vary sample temperature and pH in order to enhance the presence of specific rhodopsin intermediates.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Szundi I,Funatogawa C,Kliger DSdoi
10.1021/acs.biochem.6b00687subject
Has Abstractpub_date
2016-09-13 00:00:00pages
5095-105issue
36eissn
0006-2960issn
1520-4995journal_volume
55pub_type
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