Complexity of Bovine Rhodopsin Activation Revealed at Low Temperature and Alkaline pH.


:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. The time-resolved absorption spectra in the 330-650 nm range were analyzed by global exponential and kinetic scheme fitting methods. The results indicate an activation mechanism that is more complex than suggested previously. It involves interconnected branched pathways with two metarhodopsin I480 and two metarhodopsin II intermediates. The intermediates involved in this more complex mechanism need to be considered in spectroscopic studies that vary sample temperature and pH in order to enhance the presence of specific rhodopsin intermediates.






Szundi I,Funatogawa C,Kliger DS




Has Abstract


2016-09-13 00:00:00












  • The subcellular distribution of adenylate and guanylate cyclases in murine lymphoid cells.

    abstract::Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...


    pub_type: 杂志文章


    authors: Watson J,Nilsen-Hamilton M,Hamilton RT

    更新日期:1976-04-06 00:00:00

  • Translesional synthesis on a DNA template containing a single stereoisomer of dG-(+)- or dG-(-)-anti-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene).

    abstract::Oligodeoxynucleotides modified site-specifically with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I....


    pub_type: 杂志文章


    authors: Shibutani S,Margulis LA,Geacintov NE,Grollman AP

    更新日期:1993-07-27 00:00:00

  • Probing the Vibrio harveyi luciferase beta subunit functionality and the intersubunit domain by site-directed mutagenesis.

    abstract::While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...


    pub_type: 杂志文章


    authors: Xin X,Xi L,Tu SC

    更新日期:1994-10-11 00:00:00

  • Mapping Functionally Important Residues in the Na+/Dicarboxylate Cotransporter, NaDC1.

    abstract::Transporters from the SLC13 family couple the transport of two to four Na+ ions with a di- or tricarboxylate, such as succinate or citrate. We have previously modeled mammalian members of the SLC13 family, including the Na+/dicarboxylate cotransporter NaDC1 (SLC13A2), based on a structure of the bacterial homologue Vc...


    pub_type: 杂志文章


    authors: Colas C,Schlessinger A,Pajor AM

    更新日期:2017-08-22 00:00:00

  • Partial characterization of a tropoelastin precursor isolated from chick aorta.

    abstract::Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e.,...


    pub_type: 杂志文章


    authors: Rucker RB,Heng-Khoo CS,Dubick M,Lefevre M,Cross CE

    更新日期:1979-09-04 00:00:00

  • Intrinsic Disorder to Order Transitions in the Scaffold Phosphoprotein P from the Respiratory Syncytial Virus RNA Polymerase Complex.

    abstract::Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...


    pub_type: 杂志文章


    authors: Noval MG,Esperante SA,Molina IG,Chemes LB,Prat-Gay Gd

    更新日期:2016-03-15 00:00:00

  • Analysis of pH-induced structural changes of the isolated extrinsic 33 kilodalton protein of photosystem II.

    abstract::Structural properties of the isolated extrinsic regulatory 33 kDa protein of the water-oxidizing complex were analyzed at different pH values. It was found that (a) titrations of the buffer capacity reveal a characteristic hysteresis effect that is unique for the 33 kDa subunit and is not observed for the other extrin...


    pub_type: 杂志文章


    authors: Shutova T,Irrgang KD,Shubin V,Klimov VV,Renger G

    更新日期:1997-05-27 00:00:00

  • Fluorescence-detected circular dichroism of ethidium in vivo and bound to deoxyribonucleic acid in vitro.

    abstract::Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However...


    pub_type: 杂志文章


    authors: Lamos ML,Turner DH

    更新日期:1985-06-04 00:00:00

  • Decrease in ADP-ribosylation of HeLa non-histone proteins from interphase to metaphase.

    abstract::Variations for non-histones in the ADP-ribosylating activities of interphase and metaphase cells were investigated. 32P-Labeled nicotinamide adenine dinucleotide ([32P]NAD), the specific precursor for the modification, was used to radioactively label proteins. Permeabilized interphase and mitotic cells, as well as iso...


    pub_type: 杂志文章


    authors: Adolph KW,Song MK

    更新日期:1985-01-15 00:00:00

  • Mapping the interfacial binding surface of human secretory group IIa phospholipase A2.

    abstract::Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membrane...


    pub_type: 杂志文章


    authors: Snitko Y,Koduri RS,Han SK,Othman R,Baker SF,Molini BJ,Wilton DC,Gelb MH,Cho W

    更新日期:1997-11-25 00:00:00

  • Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters.

    abstract::NADH:ubiquinone oxidoreductase (NDH-1 or complex I) from Escherichia coli was purified using a combination of anion exchange chromatography and centrifugation in sucrose density gradient. The dependence of enzyme activity on detergent and phospholipids was studied. Artificial hexaammineruthenium reductase activity was...


    pub_type: 杂志文章


    authors: Sinegina L,Wikström M,Verkhovsky MI,Verkhovskaya ML

    更新日期:2005-06-14 00:00:00

  • Divergence in nonspecific hydrophobic packing interactions in the apo state, and its possible role in functional specialization of mitochondrial and microsomal cytochrome b5.

    abstract::The outer mitochondrial membrane isoform of mammalian cytochrome b(5) (OM b(5)) is distinguished from the microsomal isoform (Mc b(5)) by its considerably greater stability. In contrast, OM and Mc apocytochrome b(5) (apo-b(5)) exhibit similar thermodynamic stability. Contributing substantially to the greater stability...


    pub_type: 杂志文章


    authors: Cowley AB,Sun N,Rivera M,Benson DR

    更新日期:2005-11-08 00:00:00

  • Strongly altered receptor binding properties in PP and NPY chimeras are accompanied by changes in structure and membrane binding.

    abstract::Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...


    pub_type: 杂志文章


    authors: Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

    更新日期:2005-06-28 00:00:00

  • A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products.

    abstract::Octaprenyl pyrophosphate synthase (OPPs) catalyzes consecutive condensation reactions of farnesyl pyrophosphate (FPP) with five molecules of isopentenyl pyrophosphate (IPP) to generate C(40) octaprenyl pyrophosphate (OPP) which constitutes the side chain of menaquinone. We have previously reported the X-ray structure ...


    pub_type: 杂志文章


    authors: Guo RT,Kuo CJ,Ko TP,Chou CC,Liang PH,Wang AH

    更新日期:2004-06-22 00:00:00

  • Crystallographic studies of isosteric NAD analogues bound to alcohol dehydrogenase: specificity and substrate binding in two ternary complexes.

    abstract::CNAD (5-beta-D-ribofuranosylnicotinamide adenine dinucleotide) is an isosteric C-glycosidic analogue of NAD(H) containing a neutral pyridine ring. CPAD (5-beta-D-ribofuranosylpicolinamide adenine dinucleotide) is a closely related pyridine-containing analogue with the pyridine nitrogen on the opposite side of the ring...


    pub_type: 杂志文章


    authors: Li H,Hallows WH,Punzi JS,Pankiewicz KW,Watanabe KA,Goldstein BM

    更新日期:1994-10-04 00:00:00

  • Energy landscape of the Michaelis complex of lactate dehydrogenase: relationship to catalytic mechanism.

    abstract::Lactate dehydrogenase (LDH) catalyzes the interconversion between pyruvate and lactate with nicotinamide adenine dinucleotide (NAD) as a cofactor. Using isotope-edited difference Fourier transform infrared spectroscopy on the "live" reaction mixture (LDH·NADH·pyruvate ⇌ LDH·NAD(+)·lactate) for the wild-type protein an...


    pub_type: 杂志文章


    authors: Peng HL,Deng H,Dyer RB,Callender R

    更新日期:2014-03-25 00:00:00

  • An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes.

    abstract::The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...


    pub_type: 杂志文章


    authors: Venkaiah B,Viswanathan P,Habib S,Hasnain SE

    更新日期:2004-06-29 00:00:00

  • Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.

    abstract::In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...


    pub_type: 杂志文章


    authors: Zhang P,Martin PD,Purcarea C,Vaishnav A,Brunzelle JS,Fernando R,Guy-Evans HI,Evans DR,Edwards BF

    更新日期:2009-02-03 00:00:00

  • Type II Binders Targeting the "GLR-Out" Conformation of the Pseudokinase STRADα.

    abstract::Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...


    pub_type: 杂志文章


    authors: Smith RHB,Khan ZM,Ung PM,Scopton AP,Silber L,Mack SM,Real AM,Schlessinger A,Dar AC

    更新日期:2021-01-13 00:00:00

  • The structure of the bovine protein tyrosine phosphatase dimer reveals a potential self-regulation mechanism.

    abstract::The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all...


    pub_type: 杂志文章


    authors: Tabernero L,Evans BN,Tishmack PA,Van Etten RL,Stauffacher CV

    更新日期:1999-09-07 00:00:00

  • Multiple orientations in a physiological complex: the pyruvate-ferredoxin oxidoreductase-ferredoxin system.

    abstract::Ferredoxin I from Desulfovibrio africanus (Da FdI) is a small acidic [4Fe-4S] cluster protein that exchanges electrons with pyruvate-ferredoxin oxidoreductase (PFOR), a key enzyme in the energy metabolism of anaerobes. The thermodynamic properties and the electron transfer between PFOR and either native or mutated FdI...


    pub_type: 杂志文章


    authors: Pieulle L,Nouailler M,Morelli X,Cavazza C,Gallice P,Blanchet S,Bianco P,Guerlesquin F,Hatchikian EC

    更新日期:2004-12-14 00:00:00

  • Amyloid nucleation triggered by agitation of beta2-microglobulin under acidic and neutral pH conditions.

    abstract::Amyloid nucleation through agitation was studied with beta2-microglobulin, which is responsible for dialysis-related amyloidosis, in the presence of salt under acid and neutral pH conditions. First, the aggregation of beta2-microglobulin in NaCl solutions was achieved by mildly agitating for 24 h at 37 degrees C prote...


    pub_type: 杂志文章


    authors: Sasahara K,Yagi H,Sakai M,Naiki H,Goto Y

    更新日期:2008-02-26 00:00:00

  • DNA minor groove adducts formed by a platinum-acridine conjugate inhibit association of tata-binding protein with its cognate sequence.

    abstract::PT-ACRAMTU ([PtCl(en)(ACRAMTU-S)](NO(3))(2), en = ethane-1,2-diamine, ACRAMTU = 1-[2-(acridin-9-ylamino)ethyl]-1,3-dimethylthiourea) is a cytotoxic platinum-acridine conjugate previously shown to form adducts with the N3 endocyclic nitrogen of adenine in the DNA minor groove. This unusual observation and our prior det...


    pub_type: 杂志文章


    authors: Budiman ME,Bierbach U,Alexander RW

    更新日期:2005-08-23 00:00:00

  • Lysine Side-Chain Dynamics in the Binding Site of Homeodomain/DNA Complexes As Observed by NMR Relaxation Experiments and Molecular Dynamics Simulations.

    abstract::An important but poorly characterized contribution to the thermodynamics of protein-DNA interactions is the loss of entropy that occurs from restricting the conformational freedom of amino acid side chains. The effect of restricting the flexibility of several side chains at a protein-DNA interface may be comparable in...


    pub_type: 杂志文章


    authors: Baird-Titus JM,Thapa M,Doerdelmann T,Combs KA,Rance M

    更新日期:2018-05-15 00:00:00

  • NMR structures of the C-terminal segment of surfactant protein B in detergent micelles and hexafluoro-2-propanol.

    abstract::Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...


    pub_type: 杂志文章


    authors: Booth V,Waring AJ,Walther FJ,Keough KM

    更新日期:2004-12-07 00:00:00

  • 1H NMR studies of lambda cro repressor. 2. Sequential resonance assignments of the 1H NMR spectrum.

    abstract::The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...


    pub_type: 杂志文章


    authors: Weber PL,Wemmer DE,Reid BR

    更新日期:1985-08-13 00:00:00

  • Circular dichroism and electron microscopy of a core Y61F mutant of the F1 gene 5 single-stranded DNA-binding protein and theoretical analysis of CD spectra of four Tyr --> Phe substitutions.

    abstract::A core Y61F mutant of the gene 5 single-stranded DNA-binding protein (g5p) of f1 bacterial virus aggregated when expressed from a plasmid, but, after refolding in vitro, it behaved much like wild-type and may be a stability or folding mutant. Circular dichroism (CD) titrations showed the same cooperative polynucleotid...


    pub_type: 杂志文章


    authors: Thompson TM,Mark BL,Gray CW,Terwilliger TC,Sreerama N,Woody RW,Gray DM

    更新日期:1998-05-19 00:00:00

  • Enhancement of Agkistrodon piscivorus piscivorus venom phospholipase A2 activity toward phosphatidylcholine vesicles by lysolecithin and palmitic acid: studies with fluorescent probes of membrane structure.

    abstract::The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical propertie...


    pub_type: 杂志文章


    authors: Sheffield MJ,Baker BL,Li D,Owen NL,Baker ML,Bell JD

    更新日期:1995-06-20 00:00:00

  • Synthesis and characterization of fluorescently labeled bovine brain G protein subunits.

    abstract::G proteins play an important role in transmitting hormonal signals, and fluorescence techniques would be useful to study their cellular distribution and mechanisms. To prepare active fluorescent G protein Go/Gi or beta gamma subunits were reacted with fluorescein isothiocyanate (FITC) to label the alpha (F-alpha) and ...


    pub_type: 杂志文章


    authors: Kwon G,Remmers AE,Datta S,Neubig RR

    更新日期:1993-03-09 00:00:00

  • Mechanism of the reaction catalyzed by isoaspartyl dipeptidase from Escherichia coli.

    abstract::Isoaspartyl dipeptidase (IAD) is a member of the amidohydrolase superfamily and catalyzes the hydrolytic cleavage of beta-aspartyl dipeptides. Structural studies of the wild-type enzyme have demonstrated that the active site consists of a binuclear metal center positioned at the C-terminal end of a (beta/alpha)(8)-bar...


    pub_type: 杂志文章


    authors: Martí-Arbona R,Fresquet V,Thoden JB,Davis ML,Holden HM,Raushel FM

    更新日期:2005-05-17 00:00:00